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All Journal Biotika: Jurnal Ilmiah Biologi Jurnal Penelitian Tanaman Industri Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi Journal of Research and Technology Jurnal Mikologi Indonesia Journal of Science Innovare FITOFARMAKA: Jurnal Ilmiah Farmasi EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Jurnal Penelitian Tanaman Industri (Littri) Jurnal Pengabdian Masyarakat Inovatif Jurnal Pengabdian Masyarakat Inovatif

Tri Saptari Haryani

Pakuan University

Author-ID : 586954

Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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KEEFEKTIFAN FORMULA MINYAK CENGKEH DAN SERAI WANGI TERHADAP Fusarium oxysporum f.sp. vanillae PENYEBAB BUSUK BATANG VANILI MESAK TOMBE; DARMAWAN PANGERAN; TRI SAPTARI HARYANI
Jurnal Penelitian Tanaman Industri Vol 18, No 4 (2012): Desember 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n4.2012.143-150

ABSTRAKBusuk batang vanili (BBV) yang disebabkan oleh Fusariumoxysporum f.sp. vanillae (Fov) merupakan patogen penting pada vanili danmenyebabkan kerugian besar setiap tahunnya di Indonesia. Jumlahkerugian diperkirakan sebesar 3.000 ton atau sekitar US$ 16 juta.Penelitian dilaksanakan di laboratorium dan rumah kaca Kelti Hama danPenyakit, Balai Penelitian Tanaman Rempah dan Obat, Bogor pada bulanFebruari 2010 sampai Agustus 2010. Tujuan penelitian adalah untukmengetahui pengaruh formula dengan bahan aktif minyak cengkeh danserai wangi terhadap penyakit BBV dan dampaknya terhadap kehidupanmikroorganisme tanah. Dalam pelaksanaannya, kegiatan penelitian dilaku-kan dalam 3 tahap yaitu : (1) uji formula in vitro, (2) uji formula in vivo,dan (3) uji dampak formula terhadap mikroorganisme tanah. Percobaantahap 1, 2, dan 3 masing-masing terdiri atas 10, 6, dan 6 perlakuan,dan.masing-masing disusun menggunakan rancangan acak lengkap dengan3 ulangan. Materi yang dikaji dalam penelitian ini adalah (1) formuladengan bahan aktif minyak cengkeh (formula standar), (2) formula denganbahan aktif minyak cengkeh dan serai wangi (formula baru), dan (3)fungisida sintetis berbahan aktif mankozeb sebagai pembanding. Hasil ujiin vitro menunjukkan bahwa fungisida nabati CS (minyak cengkeh + seraiwangi) pada ke 3 tingkatan konsentrasi (terutama konsentrasi 400 ppm)memperlihatkan efektivitas yang tinggi dalam menghambat pertumbuhanmiselium dan produksi spora patogen Fov.F117 dibandingkan perlakuanlain dan kontrol. Hasil uji in vivo menunjukkan hal yang sama bahwafungisida nabati CS memperlihatkan efektivitas yang tinggi dalammenekan intensitas serangan patogen BBV, terutama pada dosis aplikasi 5ml/l. Dampak perlakuan terhadap kehidupan mikroorganisme tanah (fungidan bakteri) memperlihatkan bahwa penggunaan fungisida sintetismankozeb menghambat kehidupan mikroorganisme tanah 90-100% jikadibandingkan dengan kontrol. Populasi mikroorganisme tanah pada semuaperlakuan fungisida nabati lebih tinggi jika dibandingkan dengan fungisidamankozeb. Populasi mikroorganisme pada aplikasi fungisida nabati padadosis 5 ml/l tidak berbeda nyata dengan kontrol. Penelitian inimenunjukkan bahwa penggunaan fungisida nabati minyak cengkehterutama jika dikombinasikan dengan minyak serai wangi mempunyaiprospek untuk digunakan dalam pengendalian penyakit BBV denganinterval aplikasi 3-4 minggu sekali.Kata kunci : Vanilla planifolia, Fusarium oxysporum f.sp, vanillae, busukbatang vanili, minyak cengkeh, minyak serai wangiABSTRACTVanilla stem rot (VSR) disease caused by Fusarium oxysporum f.sp.vanillae (Fov) is an important disease on vanilla and causes severe lossannually in Indonesia. The total annual loss in production due to the VSRwas estimated to be 3,000 ton or about US$ 16 million. This research wascarried out in the laboratory and green house of Pest and Disease ofResearch Institute for Spices and Medicinal Crops, Bogor from February2010 until August 2010. The objective of this research was to study theeffect of formula using active compounds of clove and citronella oils onthe VSR disease and its impact on the survival of soil microorganisms.The research activities were conducted in three stages, namely (1) in vitroformula test; (2) in vivo formula test, and (3) impact test of formula on thesoil microorganisms. Materials studied in this research were (1) formulawith active compound of clove oil (standard), (2) formula with activecompound of clove and citronella oils (new formula), and (3) syntheticfungicide with mancozeb active ingredient as comparison. Every phase ofthis 3 stage experiment was arranged using completely randomized designwith three replicates. First, second, and third phases of the experimentconsisted of 10, 6, and 6 treatments, respectively. In vitro test resultscarried out in the laboratory indicated that botanical formula CS (clove andcitronella oils of the three concentration levels, especially on the 400 ppm)showed high effectiveness on inhibiting mycelium growth and sporeproduction of Fov.F117 pathogen compared to other treatments andcontrol. In vivo test in the green house indicated the same result thatbotanical fungicide CS (clove dan citronella oils) showed higheffectiveness in suppressing VSR disease infection intensity, especially on5 ml/l dosage. Application of mancozeb synthetic fungicide inhibited 90 –100% soil microorganism livelihood compared to control. However, thesoil microorganism population on all botanical fungicides was higher thanon mancozeb fungicide. The microorganism population on the botanicalfungicide of 5 ml/l dosage was not significantly different from the control(natural soil with no treatment). This research indicates that aplication ofbotanical fungicide (combination between clove and citronella oils) with3 - 4 weeks interval is prospectous to control VSR disease.Key words : Vanilla planifolia, Fusarium oxysporum, f.sp. vanillae,vanilla stem rot, clove oil, citronella oil

Isolation And Identification Of Pathogen Mushroom Types On Umbi Talas (Colocasia Esculenta (L.) Schoot) Post Harvest Fitria Dewi Sulistiyono; Tri Saptari Haryani
Journal of Science Innovare Vol 1, No 01 (2018): Journal of Science Innovare
Publisher : Universitas Pakuan

The taro tuber (Colocasia esculenta) is an important source of carbohydrates for an important energy producer whose portion has a fairly bright and profitable development prospect. Taro is not only used as a food source, it can be used for industrial purposes, for example raw materials of cosmetics and plastic. Taro is very easy to recognize and varied with other preparations because it has a distinctive flavor. Increased production is the only major consideration in taro cultivation. Efforts to increase production are influenced by limiting factors that are often experienced in the community. Such barrier is a pathogen attack both on the ground and post harvest. The purpose of this study is to isolate and identify the types of pathogenic fungi found in taro tubers. The method used is microscopic and macroscopic identifications. The results of identification with the macroscopic and microscopic observations obtained by the genus Aspergillus, Candida, Sclerotium, Fusarium, Mucor, and Rhizopus. Keywords: Taro, Identification, Pathogenic Fungi

KADAR FENOLIK DAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL RUMPUT LAUT COKLAT (Padina australis) Tri Saptari; Triastinurmiatiningsih Triastinurmiatiningsih; Bina Lohita Sari; Indah Nur Sayyidah
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 9, No 1 (2019): FITOFARMAKA | Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Rumput laut coklat Padina australis merupakan salah satu sumber daya alam laut yang keberadaannya sangat melimpah di perairan pantai Bayah, Banten Indonesia. Kandungan senyawa fenolik dan turunannya (flavonoid) berhubungan dengan aktivitasnya sebagai antibakteri dan antioksidan. Fenolik dianggap sebagai molekul dengan potensi tertinggi untuk menetralkan radikal bebas. Penelitian ini bertujuan untuk menentukan kadar fenolik dan aktivitas antioksidan ekstrak etanol P. australis menggunakan pereaksi Follin-ciocalteu dan 1,1-diphenyl-2-picryl hydrazyl (DPPH) ekstrak etanol P. australis. Hasil penelitian menunjukkan kadar fenolik sebesar 42,62 mg SAG/g serbuk dan pada konsentrasi 5000 ppm menghambat sebesar 66, 01 % radikal bebas DPPH.

EFEKTIVITAS EKSTRAK Padina australis SEBAGAI ANTIBAKTERI Escherichia coli PENYEBAB DIARE Tri Saptari Haryani; Bina Lohita Sari; Triastinurmiatiningsih Triastinurmiatiningsih
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 4, No 2 (2014): FITOFARMAKA
Publisher : Universitas Pakuan

Organisme laut merupakan sumber senyawa obat yang berpotensi besar, tetapi sampaisaat ini masih belum dimanfaatkan secara maksimal. Penelitian efektivitas ekstrak Padina

POTENSI EKSTRAK CODIUM, HALIMEDA, DICTYOTA, CHONDRUS, GLACILLARIA SEBAGAI SUMBER PIGMEN DAN ANTIOKSIDAN ALAMI Tri Saptari Haryani; Uswatun Hasanah; Oom Komala; Intan Lestari; Dina Agustina
EKOLOGIA Vol 22, No 1 (2022): EKOLOGIA : JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v22i1.4739

Macroalgae is one of the marine biological resources that has an important role in terms of economy, as a source of food and medicine that is beneficial to health. This study aims to determine the source of natural pigments and antioxidants extracts of algae Codium, Halimeda, Dictyota, Chondrus, and Glacillaria using acetone, Folin Ciocalteu, and 1,1-Diphenyl-2-Picrylhydrazil (DPPH) reagents. The method includes sampling, measuring water content by gravimetry, making extracts using maceration method, qualitative phytochemical testing, pigment source test for chlorophyll a, chlorophyll b, total chlorophyll, total phenol, and antioxidant activity. Phytochemical test results obtained that all samples contained flavonoid compounds, alkaloids, while Halimeda and Gracillaria extracts did not have terpenoids. The results of the test for the pigment source were the highest value obtained in Chondrus extract, which was 10.0038 mg/g. And from the results of antioxidant activity testing, all sample extracts were obtained including the group with moderate strength (IC50 value) ranging from 100-500).

AKTIVITAS EKSTRAK ETANOL 96% KULIT BIJI MELINJO (Gnetum gnemon) SEBAGAI ANTIBAKTERI Salmonella enteritidis Ade Kusmiati; Tri Saptari Haryani; Triastinurmiatiningsih .
EKOLOGIA Vol 19, No 1 (2019): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Salmonella enteritidis contamination in chicken meat causes chicken meat to rapidly decay. The use of chemical preservatives is very harmful, therefore the use of natural materials is recommended because it is safer to consume for humans. One of them is using Melinjo seed skin which has antibacterial power. The purpose of the study is to find out the activity of Melinjo seed bark extract (Gnetum gnemon) in inhibiting the growth of Salmonella enteritidis that can be used as a natural preservative in chicken meat. The first stage of the study was by extracting Melinjo seed bark using ethanol 96% with maceration method for 6 days. Activity test of Melnjo seed bark extract against Salmonella enteritidis using method of disc paper with a variation of the treatment of 25%, 50%, 75% and tetracycline positive control. The observed parameter is the measurement of the inhibited region Diameter (DDH) and the phytochemical test against Melinjo seed bark extract. The research results for the testing of the area Diameter of the hhibited region obtained the most effective concentration of 75% in the diameter of the area of the barrier of 10.3 mm and categorized with antimicrobial power levels of moderate sensitivity as well as can be used as natural preservatives in chicken meat. Phytochemical test results indicate the presence of compound flavonoids, tannins, saponins and triterpenoid.

KEANEKARAGAMAN ANGGREK DI CAGAR ALAM DAN TAMAN WISATA ALAM TELAGA WARNA, PUNCAK, BOGOR Siti Suryani Tahier; Tri Saptari Haryani; Sri Wiedarti
EKOLOGIA Vol 18, No 2 (2018): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v18i2.1656

The existence of orchids is often threatened with extinction that can be caused by natural damage. In addition, the continuous harvesting of orchids from nature without considering its sustainability can threaten the extinction of orchids in a region. There is a need for knowledge about the diversity of orchids so that it can be the basis of conservation in a region specifically nature reserve and Nature Park Bogor. Research using transect methods and data collection conducted using field observation techniques. The observation area consists of three locations altitude: 1400, 1500 and 1600 m above sea level . There are 27 species of orchids consisting of 19 genera. From the calculation of important value index (INP), the dominating type is found at altitude 1400 and 1500 m above sea level is Ceratostylis crassifolia, While at height and 1600 m DPL is Liparis compressa. Index diversity of type in the research site with a height of 1400 m DPL is moderate and at an altitude of 1500 and 1600 m DPL is high. The spread index is known that the pattern of spreading orchid species in three research locations has a clumped group spread pattern.

Isolasi dan Identifikasi Cendawan Patogen pada Umbi Talas (Colocasia esculenta (L.) Schott) Pasca Penyimpanan Fitria Dewi Sulistiyono; Tri Saptari Haryani
Jurnal Mikologi Indonesia Vol 4, No 2 (2020): Desember 2020
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46638/jmi.v4i2.91

Organisme Pengganggu Tumbuhan (OPT) merupakan salah satu faktor yang menentukan keberhasilan agribisnis. Pengelolaan OPT pascapanen dan pasca penyimpanan yang tepat dapat mempertahankan kualitas produk yang baik. Infeksi OPT yang sering muncul pada umbi talas adalah cendawan. Adanya jeda waktu dari pemanenan hingga ke tangan konsumen dimungkinkan adanya cendawan yang berasosiasi dengan busuk penyimpanan pada umbi talas tersebut. Tujuan dari penelitian ini adalah mengisolasi dan mengidentifikasi cendawan patogen yang terdapat pada umbi talas setelah penyimpanan. Metode yang digunakan adalah identifikasi secara makroskopis dan mikroskopis. Hasil identifikasi secara morfologi menunjukkan cendawan yang berasosiasi dengan busuk penyimpanan pada umbi talas adalah genus Mucor, Fusarium, dan Alternaria.

EFEKTIVITAS ANTIFUNGI MINYAK ATSIRI KENANGA (Cananga odorata) TERHADAP Aspergillus flavus Triastinurmiatiningsih .; Tri Saptari Haryani; Ghina Athyah Wahid
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 12, No 1 (2022): FITOFARMAKA: Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jf.v12i1.3476

Aspergillus flavus merupakan kapang penghasil utama aflatoksin yang banyak mengkontaminasi komoditi kacang-kacangan, biji-bijian, dan serealia. Bahan alami yang dapat digunakan untuk mengatasi permasalahan tersebut dengan penggunaan minyak atsiri kenanga (Cananga odorata). Tujuan penelitian ini adalah untuk mengetahui senyawa fitokimia dan menguji aktivitas antifungi dari minyak atsiri kenanga (C. odorata) terhadap A. flavus. Penentuan Konsentrasi Hambat Minimum (KHM) menggunakan metode dilusi padat dengan konsentrasi 10%, 20%, 30%, dan 40%, dan penentuan Diameter Daerah Hambat (DDH) dengan metode difusi agar sumuran menggunakan lima macam perlakuan yaitu tiga konsentrasi 40%, 50%, dan 60%, kontrol positif (Ketokonazol 50 mg/L), dan kontrol negatif (Tween 80) sebanyak 50 µl. Data hasil tes DDH kemudian dianalisis menggunakan ANOVA dengan tingkat kepercayaan 95% dan α = 0,05 dilanjutkan uji Duncan. Senyawa kimia yang terkandung dalam minyak atsiri kenanga adalah flavonoid, alkaloid, saponin dan triterpenoid. Hasil konsentrasi hambat minimum didapat pada konsentrasi 40%, sedangkan pada uji DDH, konsentrasi 60% merupakan konsentrasi yang paling optimum menghambat pertumbuhan kapang A. flavus dengan rerata daerah hambat 12,34 mm

Isolation And Identification Of Pathogen Mushroom Types On Umbi Talas (Colocasia Esculenta (L.) Schoot) Post Harvest Fitria Dewi Sulistiyono; Tri Saptari Haryani
Journal of Science Innovare Vol 1, No 1 (2018): Journal of Science Innovare, Volume 01 Number 01 2018
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v1i01.685

Co-Authors Ade Kusmiati Ardiani, Weni Arti Wahyu Utami Azzahro, Fiona Bina Lohita Sari DARMAWAN PANGERAN Dede Giwang Maelani Dina Agustina Djarot, Prasetyorini Eman eman Endrawati, Silvia Fauziah, Salsabela Sipa Fiona Azzahro Firman Rezaldi Fitria Dewi Sulistiyono Fitria Eka Resti Wijayanti Ghina Athyah Wahid Hariningsih, Yetti Indah Nur Sayyidah Intan Lestari Ismanto Ismanto Ismanto Jihan Nabilla Khaerani, Nuraliefah Kurniawati, Ulan MESAK TOMBE Moerfiah Neo Endra Lelana, Neo Endra Nurfajriah, Mutiara Siti Oom Komala Rahmad, Sukardi Sugeng Reni Purbanova Rezaldi, Firman Rifkarosita Putri Ginaris SATA YOSHIDA SRIE RAHAYU Sinta Wisma Sari Siti Suryani Tahier Sri Wiedarti Sudrajat, Cecep Sudrajat, Cecep Sukardi Sugeng Rahmad Syafrudin, Yudi Tampubolon, Jureka Tombe, Olivia Mersylia Triastinurmiatiningsih Triastinurmiatiningsih, Triastinurmiatiningsih Usman Setiawan Uswatun Hasanah Wahyu Prihatini Yudianto, Tri Yulianti Bramasto Yulianti Bramasto Zulkhoir Zulkhoir

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