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All Journal Biotika: Jurnal Ilmiah Biologi Jurnal Penelitian Tanaman Industri Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi Journal of Research and Technology Jurnal Mikologi Indonesia Journal of Science Innovare FITOFARMAKA: Jurnal Ilmiah Farmasi EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Jurnal Penelitian Tanaman Industri (Littri) Jurnal Pengabdian Masyarakat Inovatif Jurnal Pengabdian Masyarakat Inovatif
Tri Saptari Haryani
Pakuan University
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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KEANEKARAGAMAN ANGGREK DI CAGAR ALAM DAN TAMAN WISATA ALAM TELAGA WARNA, PUNCAK, BOGOR Siti Suryani Tahier; Tri Saptari Haryani; Sri Wiedarti
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 18, No 2 (2018): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v18i2.1656

Abstract

The existence of orchids is often threatened with extinction that can be caused by natural damage. In addition, the continuous harvesting of orchids from nature without considering its sustainability can threaten the extinction of orchids in a region. There is a need for knowledge about the diversity of orchids so that it can be the basis of conservation in a region specifically nature reserve and Nature Park Bogor. Research using transect methods and data collection conducted using field observation techniques. The observation area consists of three locations altitude: 1400, 1500 and 1600 m above sea level . There are 27 species of orchids consisting of 19 genera. From the calculation of important value index (INP), the dominating type is found at altitude 1400 and 1500 m above sea level is Ceratostylis crassifolia, While at height and 1600 m DPL is Liparis compressa. Index diversity of type in the research site with a height of 1400 m DPL is moderate and at an altitude of 1500 and 1600 m DPL is high. The spread index is known that the pattern of spreading orchid species in three research locations has a clumped group spread pattern.
POTENSI EKSTRAK CODIUM, HALIMEDA, DICTYOTA, CHONDRUS, GLACILLARIA SEBAGAI SUMBER PIGMEN DAN ANTIOKSIDAN ALAMI Tri Saptari Haryani; Uswatun Hasanah; Oom Komala; Intan Lestari; Dina Agustina
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 22, No 1 (2022): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v22i1.4739

Abstract

Macroalgae is one of the marine biological resources that has an important role in terms of economy, as a source of food and medicine that is beneficial to health. This study aims to determine the source of natural pigments and antioxidants extracts of algae Codium, Halimeda, Dictyota, Chondrus, and Glacillaria using acetone, Folin Ciocalteu, and 1,1-Diphenyl-2-Picrylhydrazil (DPPH) reagents. The method includes sampling, measuring water content by gravimetry, making extracts using maceration method, qualitative phytochemical testing, pigment source test for chlorophyll a, chlorophyll b, total chlorophyll, total phenol, and antioxidant activity. Phytochemical test results obtained that all samples contained flavonoid compounds, alkaloids, while Halimeda and Gracillaria extracts did not have terpenoids. The results of the test for the pigment source were the highest value obtained in Chondrus extract, which was 10.0038 mg/g. And from the results of antioxidant activity testing, all sample extracts were obtained including the group with moderate strength (IC50 value) ranging from 100-500).
AKTIVITAS EKSTRAK ETANOL 96% KULIT BIJI MELINJO (Gnetum gnemon) SEBAGAI ANTIBAKTERI Salmonella enteritidis Ade Kusmiati; Tri Saptari Haryani; Triastinurmiatiningsih .
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 19, No 1 (2019): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v19i1.1659

Abstract

Salmonella enteritidis contamination in chicken meat causes chicken meat to rapidly decay. The use of chemical preservatives is very harmful, therefore the use of natural materials is recommended because it is safer to consume for humans. One of them is using Melinjo seed skin which has antibacterial power. The purpose of the study is to find out the activity of Melinjo seed bark extract (Gnetum gnemon) in inhibiting the growth of Salmonella enteritidis that can be used as a natural preservative in chicken meat. The first stage of the study was by extracting Melinjo seed bark using ethanol 96% with maceration method for 6 days. Activity test of Melnjo seed bark extract against Salmonella enteritidis using method of disc paper with a variation of the treatment of 25%, 50%, 75% and tetracycline positive control. The observed parameter is the measurement of the inhibited region Diameter (DDH) and the phytochemical test against Melinjo seed bark extract. The research results for the testing of the area Diameter of the hhibited region obtained the most effective concentration of 75% in the diameter of the area of the barrier of 10.3 mm and categorized with antimicrobial power levels of moderate sensitivity as well as can be used as natural preservatives in chicken meat. Phytochemical test results indicate the presence of compound flavonoids, tannins, saponins and triterpenoid.
UJI PROKSIMAT DAN EFEKTIVITAS TAPE HANJELI SEBAGAI ANTIBAKTERI STAPHYLOCOCCUS AUREUS PENYEBAB PENYAKIT KULIT Haryani, Tri Saptari; Sudrajat, Cecep; Fauziah, Salsabela Sipa
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 23, No 2 (2023): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v23i2.9536

Abstract

Indonesia is rich in various kinds of traditional fermented foods that can be developed into functional foods, one of which is tape hanjeli. Tape hanjeli is a traditional food typical of the Sukabumi region, West Java, made from hanjeli seeds (Coix lacyma jobi-L) which are fermented using tape yeast. The research aims to test the proximate content and effectiveness of hanjeli tape as an antibacterial for Staphylococcus aureus which causes skin infections. Research methods include making hanjeli tape, testing the proximate content and testing the effectiveness of hanjeli tape as an antibacterial for Staphylococcus aureus (Kirby-bauer). Data analysis was carried out qualitatively and quantitatively. From the results of the proximate content test, the ash content was 0.18%, protein 8.37%, fat 7.85%, carbohydrates 22.45%. The effectiveness test results obtained inhibition zones at concentrations of 60% (1.02 mm), 70% (3.56 mm), 80% (3.06 mm) 90% (5.68 mm). The conclusion of the research stated that hanjeli tape can act as a functional food and is effective as an antibacterial for Staphylococcus aureus which causes skin disease at a concentration of 90% of 5.68 mm and is included in the moderate inhibition zone category.
UJI ANTAGONIS Trichoderma sp. TERHADAP FUNGI PATOGEN Pestalotiopsis sp. PENYEBAB PENYAKIT HAWAR DAUN PADA TANAMAN Eucalyptus Khaerani, Nuraliefah; Haryani, Tri Saptari; Lelana, Neo Endra
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 24, No 1 (2024): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v24i1.9149

Abstract

Leaf blight disease has become one of the significant ailments in Eucalyptus spp. forest plants. This disease can lead to plant growth disruption and even plant mortality. This research aims to evaluate the antagonistic abilities of several Trichoderma isolates against Pestalotiopsis fungi associated with Eucalyptus leaf blight disease. The research was conducted using the dual culture method, wherein both Trichoderma isolates and the pathogen were cultivated on the same petri dish. The obtained data were analyzed using ANOVA at a 5% significance level, followed by the Duncan test in case of significant differences. The results of BLAST analysis of pathogens causing leaf blight have the highest similarity with Pestalotiopsis uvicola, which is equal to 99.82%. The Results test antagonist show percentage inhibition Trichoderma to pathogen Pestalotiopsis biggest showed by isolate TL122 ie of 65.333%, followed with isolates TL411 and TL631 ie consecutive by 54.49% and 49.33%. The T.L.122 isolate was declared to be the most effective as a potential antagonist fungus against the Pestalotiopsis pathogen which causes leaf blight in Eucalyptus plants.
PENGENDALIAN PENYAKIT KARAT PURU PADA BIBIT SENGON (Falcataria moluccana) DENGAN PRIMING BENIH DAN FUNGISIDA NABATI DAUN MINDI (Melia azedarach) Azzahro, Fiona; Haryani, Tri Saptari; Bramasto, Yulianti
Journal of Research and Technology Vol. 6 No. 1 (2020): JRT Volume 6 No 1 Jun 2020
Publisher : 2477 - 6165

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.608 KB) | DOI: 10.55732/jrt.v6i1.133

Abstract

Production of sengon continuously improved to meet the needs of the timber industry, but increased production sengon still face the problem of that disease gall rust fungus Uromycladiumtepperianum Sacc. that is causing inhibited growth of sengon, so it needs to be eco-friendly controlled by spraying fungicide from mindi leaves which contains active ingredient azadirachtin and margocyn as antimicroorganisms. The research was conducted by using Factorial Completely Randomized Design with 2 factors and 3 replications. Priming factors (A) consisted of A0 (control), A1 (immersion), and A2 (humidification). Fungicide factors (B) consisted of B0 (control), B1 (100 grams of Mindi leaves / 2L water), B2 (150 grams of mindi leaves/2L water), B3 (200 grams of mindi leaves/2L of water), and B4 (250 grams of mindi leaves/2L water). Fungicide was given every 7 days after inoculation with 7 times of applications. The results of the research proved that the treatment A2B3 (priming with humidification and fungicides 200 grams of mindi leaves/2L of water) was an effective treatment in reducing the intensity of gall rust disease on sengon with 8,36% of intensity.
KARAKTERISASI ISOLAT BAKTERI ASAM LAKTAT ASAL TAPE HANJELI Haryani, Tri Saptari; Ismanto, Ismanto; Kurniawati, Ulan
BIOTIKA Jurnal Ilmiah Biologi Vol 21, No 2 (2023): BIOTIKA DESEMBER 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v21i2.45660

Abstract

Tape hanjeli merupakan makanan tradisional khas daerah Sukabumi, Jawa Barat, terbuat dari biji hanjeli (Coix lacryma jobi-L.) yang difermentasi menggunakan ragi. Penelitian bertujuan menguji karakterisasi probiotik isolat bakteri asam laktat asal tape hanjeli. Metode penelitian meliputi pembuatan tape hanjeli, isolasi dan identifikasi isolat bakteri asam laktat, serta karakterisasi Bakteri Asam laktat secara makroskopik dan mikroskopik. Isolasi bakteri asam laktat dilakukan dengan metode pengenceran pada media de Man Rogosa Sharpe Agar (MRS Agar). Diambil tiga pengenceran terakhir 10-5 , 10-6 dan 10-7 . Identifikasi dan karakterisasi bakteri asam laktat secara makroskopik meliputi ukuran, bentuk, warna, elevasi, margin dan opasitas koloni yang tumbuh. Identifikasi secara mikroskopik meliputi Uji Pewarnaan Gram, Uji Katalase, Uji Oksidase. Hasil penelitian diperoleh enam isolat bakteri asam laktat berbentuk batang; hasil uji pewarnaan Gram diperoleh Gram positif, pH pertumbuhan bakteri asam laktat berkisar antara 4.0 – 5.0; hasil uji katalase negatif dan uji oksidase negative. Kesimpulan menunjukkan bahwa bakteri asam laktat dari tape hanjeli diduga berasal dari genus Lactobacillus.
Potential of Hanjeli (Coix lacryma-jobi) as an antifungal Microsporum Gypseum Causes Skin Infections: Extract, Boiled Water, Kombucha, and Bath Soap Haryani, Tri Saptari; Nurfajriah, Mutiara Siti; Sudrajat, Cecep; Rezaldi, Firman; Yudianto, Tri; Ginaris, Rifkarosita Putri; Rahmad, Sukardi Sugeng
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.11744

Abstract

Job's tears (Coix lacryma-Jobi L.) are known to contain chemical compounds of the alkaloid, flavonoid and tannin groups that have the potential as antifungals. Microsporum gypseum is one of the dermatophyte fungi that causes dermatitis, a disease that infects skin of both animals and humans. The study aims to test the activity and determine optimal concentration of Job's tears seed extract, Job's tears seed boiled water, Job's tears leaf kombucha, and Job's tears seed extract bath soap formulation as antifungals against Microsporum gypseum which causes skin disease infections. The research method used agar diffusion with the well technique (Agar Well Diffusion), the research parameters included phytochemical tests of the extract, measuring diameter inhibition area. The results phytochemical test obtained alkaloid compounds, and results of activity test of the Job's tears seed extract formulation, Job's tears seed boiled water, Job's tears leaf kombucha, and Job's tears seed extract bath soap obtained optimal diameter of the inhibition area overall at an extract concentration of 40%. Conclusion,  activity of ethanol extract of hanjeli seeds, boiled water of hanjeli seeds, hanjeli leaf kombucha, and hanjeli seed extract bath soap at a concentration of 40% have antifungal activity against Microsporum gypseum which causes skin diseases.
Morphological and Molecular Characterization of Pestalotiopsis sp. Associated with Leaf Spot Disease in Eucalyptus pellita Endrawati, Silvia; Haryani, Tri Saptari; Lelana, Neo Endra
Journal of Science Innovare Vol 8, No 1 (2025): Journal of Science Innovare, Volume 08 Number 02 2025
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v8i1.12814

Abstract

Leaf spot disease is one of the most common foliar infections affecting Eucalyptus pellita, a fast-growing tree species widely cultivated for pulp and paper industries in Indonesia. This study aimed to isolate and identify the fungal pathogen associated with the disease through morphological and molecular characterization. Symptomatic leaves were collected from E. pellita stands in KHDTK Parungpanjang, and fungal isolation was performed on Potato Dextrose Agar (PDA). Morphological observations revealed that the isolated fungus exhibited white colonies with purple pigmentation at the center and conidia containing four septa—typical characteristics of Pestalotiopsis species. Molecular identification was conducted using Internal Transcribed Spacer (ITS) region sequencing of rDNA, followed by BLAST analysis against the NCBI GenBank database. Sequence alignment and phylogenetic tree reconstruction confirmed that the isolate (PT.E002) shared 100% similarity with Pestalotiopsis mangiferae, P. clavispora, P. maculans, and P. microspora. The phylogenetic analysis indicated a close relationship between the PT.E002 isolate and P. maculans. These results validate the presence of Pestalotiopsis sp. as the main pathogen associated with leaf spot disease in Eucalyptus pellita. The integration of morphological and molecular analyses enhances the accuracy of pathogen identification, contributing valuable insights for forest disease management and sustainable Eucalyptus cultivation programs.
Training In Manufacturing Multipurpose Eco-Enzyme Liquids for Farmers Group Djarot, Prasetyorini; Triastinurmiatiningsih; Haryani, Tri Saptari; Prihatini, Wahyu; Moerfiah; Ismanto; Rahayu, Sata Yoshida Srie; Sudrajat, Cecep
Jurnal Pengabdian Masyarakat Inovatif Vol. 2 No. 1 (2024): JPMI (Jurnal Pengabdian Masyarakat Inovatif)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jpmi.v2i1.113

Abstract

Eco-enzyme is an active ingredient from organic waste that can be used for various daily purposes such as fertilizer, herbicide, cleaning fluid, and even medicine, this is because it contains Lactobacillus, Asotobacter xylinum, yeast fungi, and lactic acid bacteria, enzymes (such as protease, amylase, lipase), minerals, and secondary metabolites (such as polyphenols, alkaloids, antioxidants). By utilizing ecoenzymes optimally, it will support the use of household organic waste with an environmentally friendly concept. And this is also an effort to reduce disruption to the environment due to landfills, which can be done by utilizing household organic waste to make eco-enzymes. The aim of this service is to provide training in making eco-enzymes as a multi-purpose liquid which will have an impact on waste utilization. Organic which is oriented towards maintaining a clean environment. The first method used was socialization and continued with presentation of material about what eco-enzymes, why we need to develop them, how to make eco-enzymes and how to use them. The material was delivered through a presentation using a projector and the production was carried out by demonstrating how to make eco-enzymes. This activity was carried out by delivering presentation material about eco-enzymes. The training was carried out at the homes of Tanah Baru Village residents with the permission of the Village Head. The training participants were farmers of the Tanah Baru Subdistrict Farmers Group, totalling 20 people and several village officials.  The training was held on June 13 2022 which included counselling and demonstrations on making eco-enzyme solutions from fruit peels and vegetable waste. The training participants seemed very enthusiastic by asking questions and having lots of discussions about eco-enzymes and their uses, participants also actively participated in manufacturing activities.
Co-Authors Ade Kusmiati Ardiani, Weni Arti Wahyu Utami Azzahro, Fiona Bina Lohita Sari DARMAWAN PANGERAN Dede Giwang Maelani Dina Agustina Djarot, Prasetyorini Eman eman Endrawati, Silvia Fauziah, Salsabela Sipa Fiona Azzahro Firman Rezaldi Fitria Dewi Sulistiyono Fitria Eka Resti Wijayanti Ghina Athyah Wahid Hariningsih, Yetti Indah Nur Sayyidah Intan Lestari Ismanto Ismanto Ismanto Jihan Nabilla Khaerani, Nuraliefah Kurniawati, Ulan MESAK TOMBE Moerfiah Neo Endra Lelana, Neo Endra Nurfajriah, Mutiara Siti Oom Komala Rahmad, Sukardi Sugeng Reni Purbanova Rezaldi, Firman Rifkarosita Putri Ginaris SATA YOSHIDA SRIE RAHAYU Sinta Wisma Sari Siti Suryani Tahier Sri Wiedarti Sudrajat, Cecep Sudrajat, Cecep Sukardi Sugeng Rahmad Syafrudin, Yudi Tampubolon, Jureka Tombe, Olivia Mersylia Triastinurmiatiningsih Triastinurmiatiningsih, Triastinurmiatiningsih Usman Setiawan Uswatun Hasanah Wahyu Prihatini Yudianto, Tri Yulianti Bramasto Yulianti Bramasto Zulkhoir Zulkhoir