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All Journal Biodidaktika : Jurnal Biologi dan Pembelajarannya Scholaria: Jurnal Pendidikan dan Kebudayaan BIOEDUKASI SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan CARADDE: Jurnal Pengabdian Kepada Masyarakat Jurnal Biotek Jurnal Inovasi Pendidikan dan Sains GUYUB: Journal of Community Engagement Sciening : Science Learning Journal Biopedagogia Indonesian Biodiversity Journal Indonesian Biodiversity Journal KREATIF Jurnal Pengabdian Masyarakat Sains dan Teknologi Bioma : Jurnal Ilmiah Biologi
Tika Putri Agustina
Universitas Negeri Manado
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OPTIMIZING FIXATION DURATION FOR ENHANCED CLARITY IN Pediculus humanus-capitis WHOLE MOUNT PREPARATIONS USING 10% KOH AT 70°C agustina, Tika putri; Rukmana, Musma; Hasmiati; Watung, Fernando Andre
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2024): April 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i1.1041

Abstract

This study evaluates the optimal fixation duration using 10% potassium hydroxide (KOH) at 70°C for whole mount preparations of Pediculus humanus capitis (head lice). Accurate head lice identification is crucial for effective public health management. Specimens were fixed for 20, 25, 30, 35, and 40 minutes at 70°C, then processed through rinsing, acetic acid treatment, dehydration, and clearing before mounting for microscopic examination. Results showed that 25 minutes of fixation provided the clearest and most detailed preparations. This duration effectively achieved tissue dehydration and clarification without significant damage. Fixation for 20 minutes was insufficient, resulting in opaque specimens due to incomplete KOH diffusion. Conversely, 40 minutes of fixation caused over-fixation, leading to tissue damage and poorer color quality. The study underscores the importance of finding the right fixation duration. While shorter times are inadequate, longer durations can degrade specimen quality. A 25-minute fixation at 70°C is optimal for balancing clarity and structural preservation, improving head lice identification. This method enhances the quality of whole mount preparations and provides practical guidance for accurate and reliable ectoparasite examination in clinical and research settings.
Co-Authors Aisyiah Restutiningsih Putri Utami Ermita Ermita Femmy Roosje Kawuwung Fernando Andre Watung Fitrianingrum, Aufa Maulida Hasmiati Hasmiati Hasmiati Hasmiati Hasmiati Kurniahtunnisa Kurniahtunnisa Lestari, Kurnia Manaari, Chaleb Paul Margaret Pandaleke Maria Yasinta Manuel Mellyatul Aini Melyatul Aini Musma Rukmana Musma Rukmana Musma Rukmana Mustapa Mustapa