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All Journal Jurnal Teknologi Dan Industri Pangan Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Jurnal Biotek Medisiana Indonesia Majalah Kedokteran Journal of Medicine and Health Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Pharmaceutical Sciences and Research (PSR) Cakradonya Dental Journal Journal of Clinical Microbiology and Infectious Diseases Journal of Innovative and Creativity Journal of Medicine and Health Cermin Dunia Kedokteran Biodiversitas Journal of Biological Diversity
Mardiastuti Mardiastuti
Department Of Clinical Microbiology, Faculty Of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Challenges for Legionella pneumophila detection in Indonesia Rivia Gina Rahmawaty; Mochamad Helmi Aziz; Chairinda Dachwan; Erie Yuwita Sari; Ibnu Agus Ariyanto; Mardiastuti Wahid
Journal of Clinical Microbiology and Infectious Diseases Vol. 2 No. 2 (2022): Available Online: December 2022
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v2i2.35

Abstract

Legionella pneumophila (L. pneumophila) is widely known to cause respiratory illness outbreaks and remains underdiagnosed, including in Indonesia. Several diagnostic methods are available, yet none have been implemented as a routine diagnostic panel in most clinical microbiology laboratories in Indonesia. The urine antigen test is the cheapest and easy to perform. However, it only detects serogroup 1 of L. pneumophila, creating a blind spot for non-serogroup 1. Culture is the gold standard, but its sensitivity and turnaround time makes culture less feasible in a clinical setting. The direct fluorescent antibody is rapid, nonetheless, expertise and experience are needed to increase the sensitivity. Molecular methods, while very sensitive, cannot rule out contamination since the bacteria are mostly found in water. In order to validate which best method to be performed in Indonesia, a nationwide surveillance and validation study should be performed.
Knowledge and practice of COVID-19 pandemic prevention in a community in Jakarta Wahid, Mardiastuti H; Kiranasari, Ariyani; Ningsih, Ika; Sari, Meutia Kumala; Aziz, Mochamad Helmi
Journal of Clinical Microbiology and Infectious Diseases Vol. 4 No. 1 (2024): Available online: June 2024
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v4i1.55

Abstract

Introduction: COVID-19 is an emerging infectious disease that causes a pandemic with high morbidity and mortality due to its wide transmission. This situation should be prevented not only by the government but also by the community. Efforts have taken place to prevent COVID-19 transmission including the obligation to wear masks, wash hands, keep a distance, stay at home, avoid crowds, and administer the vaccination. This study aims to examine the communities’ knowledge, perception, and practice in preventing the spread of the COVID-19 pandemic. Methods: This is a descriptive cross-sectional study in a suburban area in Jakarta. Ninety-seven respondents participated in this study. Data was collected by filling questionnaire via G-form and analyzed descriptively.   Results: A total of ninety-seven respondents participated in this study with aged 18-73 years. In terms of knowledge about COVID-19, the majority of the respondents know the etiology, symptoms, transmission, and the way to prevent the disease. Only less than half of them understand that corpses are capable of transmitting COVID-19. However, this research found a lack of implementation of practice in preventing COVID-19 transmission because only 62.9% of respondents always bring hand sanitizer/ABHS. Only a small percentage change clothes after leaving the house, do exercise, and take vitamins. Conclusion: Although the community has sufficient knowledge and a good perception of COVID-19 prevention, rigorous effort should be provided to enhance the quality of practice of the community, to prevent the spread of COVID-19.
HUBUNGAN ANTARA SELF-EFFICACY TERHADAP PRESTASI AKADEMIK PADA MAHASISWA KEDOKTERAN GIGI (TINJAUAN PUSTAKA) Nurul Husna; Luthfi Saiful Arif; Mardiastuti Wahid
Cakradonya Dental Journal Vol 14, No 2 (2022): Agustus 2022
Publisher : FKG Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v14i2.29954

Abstract

Self-efficacy merupakan salah satu bagian dari pilar utama dalam social cognitive theory (SCT) yang dikembangkan oleh Albert Bandura. Berbagai studi melaporkan bahwa self-efficacy berkaitan dengan prestasi akademik mahasiswa. Tinjauan pustaka ini membahas konsep self-efficacy mulai dari definisi, kategori, aspek, peran, faktor yang mempengaruhi, hubungan antara self-efficacy dengan prestasi akademik dan alat ukur yang digunakan untuk menilai self-efficacy pada mahasiswa kedokteran gigi. Kata kunci yang digunakan dalam pencarian meliputi self-efficacy, academic performance, dental student and dentistry pada database NCBI, Sage Publication, Science Direct, PubMed, Google Scholar dan sumber terpercaya lainnya. Dari hasil penelurusan didapatkan bahwa hanya satu literatur dari Kolombia yang membahas hubungan self-efficacy dengan academic performance pada mahasiswa kedokteran gigi. Telusur literatur menunjukkan beberapa alat ukur yang dapat digunakan untuk menilai self-efficacy, yaitu General Self-Efficacy Scale, The Collage Academic Self-Efficacy Scale, The Academic Self-Efficacy, dan Collage Self-Efficacy Inventory. Alat ukur ini menjadi tolak ukur untuk mengetahui tinggi atau rendahnya self-efficacy seseorang. Mahasiswa dengan self-efficacy tinggi memiliki kemampuan dalam mengatur, melaksanakan, dan menyelesaikan masalah yang berkaitan dengan tugas belajar, serta berkeyakinan dapat menyelesaikan tugas dengan baik. Pengetahuan tentang tingkat self-efficacy mahasiswa dapat dijadikan sebagai prediktor terhadap keberhasilan akademik mahasiswa tersebut.
Comparison of Students’ Characteristics, Self-Motivation, and Readiness of Self-Directed Learning Implementation among Medical Students at Maranatha Christian University Rimonta F Gunanegara; Mardiastuti H Wahid; Indah S Widyahening
Journal of Medicine and Health Vol 1 No 5 (2017)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28932/jmh.v1i5.541

Abstract

Self-Directed Learning (SDL) is an important skill that must be achieved by medical students.The aim of this study is to identify the level of self-motivation and SDL readiness in the medicalstudents as well as to identify factors affecting SDL. This is is a mixed method research,involving first-year and clinical year medical students. A quantitative research is conducted bydistributing self-motivation (MSLQ) and SDL questionnaire (SDLRS). A total sampling isapplied to select the respondents. Furthermore, focus group discussion (FGD) on students andtutors/preceptors is carried out. Informants are chosen by purposive sampling method. Thisresearch reveals that most of medical students have a good level of self-motivation but a lowlevel of SDL readiness. Nevertheless, the mean scores of SDL readiness in both groups showsno significant differences. The research also identifies four major factors affecting the SDLreadiness, namely the students’ characteristics, learning process, the role of tutors/preceptorsand supporting facilities for learning. There is no significant difference between SDL readinessof the first-year and clinical year of medical students. Unprepared students’ characteristics,sub-optimal learning process, unsupported role of tutors/preceptors and inadequate learningresources are found to be the mayor factors influencing SDL readiness.Keywords: self-directed learning readiness; self-motivation; problem-based learning
Exploring The Self-Regulated Learning Implementation On Problem-Based Learning: A Case Study Desyani Aviciena; Diantha Soemantri; Mardiastuti Wahid; Ardi Findyartini; Estivana Felaza
Journal of Innovative and Creativity Vol. 5 No. 2 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i2.698

Abstract

Latar belakang: Seorang mahasiswa kedokteran harus memiliki kemampuan sebagai pembelajar dewasa yang mawas diri dan mampu belajar sepanjang hayat. Demi mencapai hal tersebut, salah satu strategi pembelajaran yang digunakan saat ini di pendidikan kedokteran yaitu Problem Based Learning (PBL). Pada proses PBL mahasiswa dituntut untuk dapat mengatur dirinya dalam pembelajaran atau memiliki kemampuan Self-regulated Learning (SRL) untuk mencapai tujuan pembelajaran. Tujuan: Penelitian ini bertujuan untuk mengeksplorasi implementasi SRL pada PBL dan faktor yang mempengaruhinya. Metode: Penelitian ini menggunakan metode penelitian kualitatif dengan pendekatan studi kasus. Responden dipilih menggunakan teknik maximum variation sampling. Diskusi kelompok terfokus pada mahasiswa dan staf pengajar dilaksanakan untuk mengeksplorasi implementasi SRL pada PBL. Sebagai triangulasi data dilakukan pula observasi proses PBL. Hasil: Terdapat beberapa faktor yang mempengaruhi implementasi SRL pada PBL yaitu usaha staf pengajar, institusi, mahasiswa dan karakteristiknya, serta faktor lain diantaranya adalah skenario, materi, capaian pembelajaran, keberadaan teman dalam proses PBL. Kesimpulan: SRL dapat diimplementasikan dalam pendekatan PBL melalui peran serta staf pengajar, institusi dan mahasiswa, sehingga PBL dapat dimanfaatkan untuk melatih kemampuan SRL mahasiswa agar mampu belajar sepanjang hayat.
The Characterization of Nanoemulsion Gel of Tobacco Leaves Extracts in Variation of Tween 80 and Sorbitol as Surfactant and Cosurfactant Kori Yati; Gozan, Misri; Jufri, Mahdi; Wahid, Mardiastuti
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 11 No. 1 (2026)
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/farmasains.v11i1.44053

Abstract

Research showed the potential of tobacco (Nicotiana tobacco) leaves as an antifungal. The tobacco leaves (Nicotiana tobacco) have been studied to have antifungal activity. To facilitate the use of the extract, the tobacco leaf extract was formulated into a nanoemulsion gel using Virgin Coconut Oil (VCO) as an oil phase and Tween 80: sorbitol as a surfactant and co-surfactant.  Tobacco leaves were soaked in ethanol using the maceration method, and then the liquid was taken out with a rotavapor machine. After that, the mixture was heated in a water bath (t = 85ºC) to make it thicker. Tobacco extract was identified and formulated in a nanoemulsion gel. Nanoemulsion gel was made into 3 formulas with different concentrations of Tween 80 and sorbitol. Nanoemulsion gel was evaluated for organoleptic properties, pH, viscosity, cycling test, surface tension, Transmission Electron Microscopy (TEM), and particle size. Result of evaluation of best formula showed pH 4.52, viscosity 365-573 mPa.s, surface tension 28450±0.21 N/m, morphology spherical shape, particle size 55.1±1.3 nm, polydispersity index 0.236±0.003, and zeta potential -5.16±0.35 mV (n=3). Based on the results of the research, it can be concluded that the most stable formula of tobacco leaves nanoemulsion was formula R1, with Virgin Coconut Oil (VCO) at 6% and a surfactant-to-cosurfactant ratio of 20%:10%. Statistical analysis results for all evaluations showed that all formulas have significant differences (p ≤ 0.05).
Onikomikosis yang Disebabkan oleh Kodamaea ohmeri pada Seorang Pasien dengan Virus Imunodefisiensi Manusia (HIV) – Laporan Kasus: Laporan Kasus Nadliroh, Siti; Wahid, Mardiastuti; Sjatha, Fithriyah; Haliman, Cliff Clarence; Widaty, Sandra; Mutiara, Rizka; Pratama, Aditya Indra; Asri, Rizkia Chairani; Adawiyah, Robiatul; Kusumaningrum, Ardiana
Cermin Dunia Kedokteran Vol 53 No 07 (2026): Juli 2026 - CDK 355
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v53i07.1895

Abstract

Introduction: Onychomycosis is a fungal nail infection usually caused by dermatophytes. Kodamaea ohmeri is a rare fungus that has been reported as a pathogen capable of causing onychomycosis and systemic infections. This yeast-like fungus is often misidentified as Candida sp. A complete identification is required for accurate results. Case: A 40-year-old male with HIV presented with yellow and brittle fingernails for 2 months. KOH examination of nail scrapings was performed, followed by fungal culture. The isolate was identified in parallel using the Integral System Yeast Plus, Vitek-2, MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry), and sequencing, and the result was Kodamaea ohmeri. The patient received oral itraconazole and topical ketoconazole. Discussion: Onychomycosis in HIV patients is caused by low immunity, high viral load, and low CD4 values. In this case, K. ohmeri was successfully identified using Vitek-2, MALDI-TOF, and ITS (Internal Transcribed Spacer) area sequencing, while the Integral System Yeast Plus misidentified the isolate as Candida tropicalis. There was clinical improvement in the nail after treatment. Conclusion: Onychomycosis in HIV patients can be caused by Kodamaea ohmeri. Identification can be done with Vitek-2 and MALDI-TOF.
Probiotic and genomic evaluation of Lactiplantibacillus plantarum T31 isolated from tempoyak in mung bean substrate QONITA GINA FADHILAH; AUDREY ELIZABEHT; MELINDA FALENCIA; IMAN SANTOSO; ANDI EKO MARYANTO; NIARSI MERRY HEMELDA; MARDIASTUTI H. WAHID
Biodiversitas Journal of Biological Diversity Vol. 26 No. 9 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260916

Abstract

Abstract. Fadhilah QG, Elizabeht A, Falencia M, Santoso I, Maryanto AE, Hemelda NM, Wahid MH. 2025. Probiotic and genomic evaluation of Lactiplantibacillus plantarum T31 isolated from tempoyak in mung bean substrate. Biodiversitas 26: 4435-4444. Traditional fermented foods in Indonesia contain diverse beneficial microorganisms, including Lactic Acid Bacteria (LAB), which play an important role. LAB T31 has been isolated from tempoyak and evaluated for probiotic characters. In this research, the isolate was evaluated as a potential probiotic in a mung bean substrate to develop a healthy fermented drink. The result showed that LAB T31 grew well on the mung bean substrate. The population increased from 107 CFU/mL to 109 CFU/mL after 24 hours of incubation. The viability results showed that the LAB T31 population remained 10? CFU/mL after 30 days at 10°C. Cell-free filtrate of fermented mung bean substrate showed antibacterial activity against the tested bacteria. The diameter of inhibition increases from 24 hours (15-17 mm) to 48 hours (16-18 mm) of incubation. Whole genome sequences (WGS) of the isolate were performed using DNBseq technology, and the re-identification using JspeciesWS and FastANI showed that the strain belonged to Lactiplantibacillus plantarum. Analysis of the antibacterial assay test result showed that the LAB T31 genome encoded an antimicrobial peptide known as plantaricin. The hydrophobicity and auto-aggregation reached 38.86% and 36.92%, respectively, after 24 hours of incubation. Functional annotation reported adhesin genes, and the isolate did not suggest hemolytic activity on blood agar. These results supported WGS analysis using KEGG since the reported genes are not related to hemolytic ability and virulence factors. Moreover, antimicrobial resistance analysis showed that the isolate had a vanY gene, with a similarity level of 33.33%. LAB T31 was a potential isolate as a probiotic agent in the mung bean substrate.
Co-Authors Agus Sjahrurachman Agustin Indrawati Akbar, Resti Rahmadika ANDI EKO MARYANTO Andi Yasmon Angga W Lokeswara, Angga W Ardi Findyartini Ardiana Kusumaningrum Ariyani Kiranasari Asri, Rizkia Chairani AUDREY ELIZABEHT Augustine Natasha Aziz, Mochamad Helmi Azmier Adib Azmier Adib Benny Logawa Betty Ernawati Chairinda Dachwan Conny Tjampakasari Desyani Aviciena Diantha Soemantri Effendi, Evita Erie Yuwita Sari Fathan, Hafizah Felaza, Estivana Fera Ibrabim Fera Ibrahim Fera lbrahim Haliman, Cliff Clarence Ibnu Agus Ariyanto Ika Ningsih IMAN SANTOSO Indah S. Widyahening Ingrid S. Surono Kori Yati Kori Yati Kori Yati Lusi Dwita Lusi Putri Dwita Luthfi Saiful Arif Mahdi Jufri MELINDA FALENCIA Misri Gozan Mochamad Helmi Aziz Mursinah Mursinah Mursinah, Mursinah Mutiara, Rizka Nadliroh, Siti Ni Made Adi Tarini Niarsi Merry Hemelda Nurul Husna Oliver E Yausep, Oliver E Pratama, Aditya Indra Pratiwi Pujilestari Sudarmono QONITA GINA FADHILAH Raksheeth Agarwal, Raksheeth Retno Asti Werdhani Retno Kadarsih Rimonta F Gunanegara Rimonta F Gunanegara, Rimonta F Rivia Gina Rahmawaty Robiatul Adawiyah Samsuridjal Djauzi Sandra Widaty Sari, Meutia Kumala Sharon Hanmy Angel, Sharon Hanmy Sitohang, Irma Bernadette S. SJATHA, FITHRIYAH Tjahjani M. Sudiro TJAHJANI MIRAWATI SUDIRO V. P. Anny Yeva Rosana Zaenab Zaenab