Aspriyanto, Didit
Department Of Dentomaxillofacial Radiology, Faculty Of Dentistry, Universitas Lambung Mangkurat, Banjarmasin, Indonesia, 70236

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THE EFFECT OF TOMAN (Channa micropeltes) FISH EXTRACT ON EPITHELIAL THICKNESS IN DIABETES MELLITUS WOUND HEALING (In Vivo Study on the back of male Wistar rat (Rattus novergicus)) Audina, Nina; Nindia Carabelly, Amy; Aspriyanto, Didit
Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

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Background: Re-epithelialization is the process of formation of new epithelium which is important in wound healing process, the faster the process then the sooner the process of wound healing. Toman fish (Channa micropeltes) is trusted as an alternative medicine to accelerate wound healing. Toman fish contains albumin which acts an antioxidant. Antioxidant can reduce Reactive Oxygen Species (ROS) which increased at state of hyperglicemia in people with diabetes mellitus (DM), Thus accelerating the process of Re epithelialization on diabetes wound. Purpose: The Purpose of this research was to prove that fish extract Toman dose 16 ml/Kg body weight effect on thickness of the epithelium of Wistar Rat Injuries Induced DM are seen on the 2, 4 and 8 day. Material and methods: This research was true experimental design with posttest-only with control design with complete random design. This research used 27 Wistar rats are divided into 9 treatment groups, Toman fish extract dose 16 mL/Kg BW, Haruan fish extract 13,54 mL/Kg BW, and BR2 feed only. The calculation of epithelial thickness was calculates using the ImageJ software application by measuring histopathological results. Research result: The result of Two way Anova test and Post Hoc LSD test showed that there was a significant difference of effect on group Toman fish extract 16 mL/Kg BW with Haruan fish extract 13,54 mL/Kg BW and BR2 feed only to increase the thickness of the wound of epithelium of Wistar rat Induced DM. Toman fish extract showed the highest mean of epithelial thickness with 2nd day (76,69 µm), 4th day (99,21 µm) and 8th day (124,23 µm). Conclusion: There was effect of Toman fish extract dose 16 mL/Kg BW on increased epithelial thickness of Wistar rats wound on induced diabetes mellitus are seen on the days 2, 4 and 8.
THE CHANGE OF SALIVARY pH AFTER RINSING WITH FUJI APPLE (Malus sylvestris) EXTRACT 100% ON CHILDREN AGED 8-10 YEARS. (Review onstudents in SDN Pengambangan 3 Banjarmasin) Dwiyanti, Dian; Aspriyanto, Didit; Erlita, Isyana
Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

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Background: The age of 8-10 is the age group with highest number of dmf-t in children. The normal salivary pH can prevent the formation of caries. Fuji apple has contents that are antibacterial such as flavonoid, catechin and tannin that reduce the production of salivary acid by Streptococcus mutanbactery. Purpose: To analyze the difference change of pH saliva before and after rinsing with Fuji apple (Malus sylvestris) extract100% and Chlorhexidine 0,2% in oral cavity of children aged 8-10 years old. Method: This study used quasi experimental method with pre-post test control group design. The samples were determined by purposive random sampling on children aged 8-10 years old in SDN Pengambangan 3 Banjarmasin with the total of 63 people. Samples were divided to 3 treatment groups which are rinsing with Fuji apple (Malus sylvestris) extract 100%, Chlorhexidine 0,2% and aquades. Result: The result of One-way Anova test showed that there was difference on the three treatment groups (p=0,0001). The result of Posthoc Bonferroni test showed that there was significant difference between Fuji apple (Malus sylvestris) extract 100%, Chlorhexidine 0,2% (p=0,016). There was significant difference between Fuji apple (Malus sylvestris) extract 100% and Aquades (p=0,0001) and between Chlorhexidine 0,2% andaquades (p=0,0001). The increase of salivary pH was found in three groups treatment. Conclusion: There is significant difference between groups that rinse with Fuji apple (Malus sylvestris) extract and Chlorhexidine 0,2% in children aged 8-10 years old.
TOTAL COLONY CHARACTERIZATION OF AEROBIC BACTERIA OF THE ORAL CAVITY ON PEATLANDS WATER AND PDAM WATER Senggara, Bayu Yordha; Tri Putri, Deby Kania; Aspriyanto, Didit
Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

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Background: South Kalimantan peatland water is approximately 8000 km2. Barito Kuala has 40,858 hectares or 90% of the area classified as peatland water. Society uses peatlands water with acidic pH(3.5-4.5) for everyday purposes including rinsing and brushing teeth. The acidic nature of peatland water can affect the growth of bacterial colonies in the oral cavity so that the acidic conditions of the oral cavity increases and the effect on the occurrence of dental caries. In addition PDAM water is water from river water that is processed through several stages to become clean water after filtration and disinfection stages to eliminate microorganisms. Objective: this study aims to determine the characterization of the number of colonies of aerobic bacteria of the oral cavity on peatland water and PDAM water. Methods: This study used quasi experimental method with post test only control group design. The study sample consisted of 30 respondents. Research materials were 4ml of a result of the salivary gargle on peatland water and PDAM water then the number of aerobic bacteria colonies were calculated by the method of TPC (Total Plate Count) and characterization of aerobic bacteria was done by using catalase test, test MSA and coagulase test. Results: This study showed that there were 1653 CFU/ml of aerobic aerobic colony, whereas in water of PDAM there were 1264 CFU/ml of aerobic colonies. In the characterization tests of aerobic bacteria colonies showed that there were Staphylococcus aureus and Staphylococcus epidermidis bacteria on gargle water of peatland or PDAM water. Independent t-test results showed significant differences in aerobic bacterial colonies in peatland water and PDAM water (p = 0.001)(p = 0.002)(p <0.05). Conclusion: Based on this study it can be concluded that the number of Staphylococcus aureus and Staphylococcus epidermidis found in the oral cavity of children saliva who gargled with peatland water are higher than the number of Stapylococcus aureus and Staphylococcus epidermidis found in the saliva of children who gargle with PDAM water.
THE COMPARISON OF PLAQUE INDEX BEFORE AND AFTER RINSING WITH FUJI APPLE (Malus sylvestris) EXTRACT0 100% AND CHLORHEXIDINE 0,2% IN CHILDREN AGED 8-10 YEARS Ihsan, Muhammad Fauzan; Aspriyanto, Didit; Erlita, Isyana
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5371

Abstract

Background:The mixed dentition occurs in children aged 8-10 years, the change from deciduous teeth to permanent teeth. The children at this age is susceptible to oral disease because they favor to consume cariogenic foods that can cause the buildup of plaque on the teeth. Plaque is a gray-ish yellow substance that is firmly attached to the tooth surface. Plaque can be controlled with chemical treatment such as using a mouthwash. Chlorhexidine 0,2% is the most potential antimicrobial mouthwash in reducing plaque but chlorhexidine 0,2% has side effects to the oral cavity so herbal ingredients begin to be an alternative choice. Fuji applet (Malus sylvestris) contains flavonoids and tannins that are antibacterial to inhibit plaque growth. Purpose: To know the comparison of plaque index before and after rinsing with Fuji apple(Malus slyvestris) extract 100% solution and chlorhexidine 0,2% in children aged 8-10 years old.Method: This study used quasi experimental with pre-posttest with control group design The total sample was 62 people, consisted of 2 groups. Results: In the study, there was a greater decrease in plaque index score in rinsing group with Fuji apple(Malus slyvestri) extract 100% than with chlorhexidine 0.2%. The plaque index score decreased in the treatment group by 4.7% and in the control group only 3.1%. Conclusions: There are differences in plaque index scores before and after rinsing with Fuji apple(Malus sylvestris) extract 100% with chlorhexidine 0,2% in children aged 8-10 years old.
THE EFFECTS OF PANORAMIC RADIOGRAPHY ONGINGIVAL CREVICULAR FLUIDVOLUME IN WISTAR RATS WITH PERIODONTITIS Ramadhanty, Aina; Aspriyanto, Didit; Oktiani, Beta Widya
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10632

Abstract

ABSTRACTBackground: Panoramic radiography is one of the most commonly used radiographic diagnostic tools. Panoramic radiography exposure can increase the GCF flow because of increasing vascular permeability.GCF flow can also increase in periodontitis conditions. Objective: The purpose of this study was to analyze the effects of 1, 2, and 3 times exposure of panoramic radiography to the volume of GCF in wistar rats with periodontitis. Method: This research was true experimental with post test onlycontrol group design. Thesampling technique used simple random samplingthat obtained 25 wistar rats comprising of 5 wistars from each group in which the GCF samples of each group was collected from. Results: The average number of GCF inhealthy rats groupthat was not exposed to panoramic radiographywas 0.114 µL, the periodontitis rats group that was not exposed to panoramic radiography was 0.246 µL, the periodontitis rats group that was exposed1 time was 0.286 µL, the group of periodontitis rats exposed 2 times was 0.294 µL, and periodontitis group of rats exposed 3 times was 0.374 µL. The results of the One-Way Anova and Post Hoc Bonferroni tests stated that there is a significant increase in the volume of GCF between the control group and all other treatment groups, as well as between the non-exposed periodontitis group and the periodontitis group with 3 times exposure. Conclusion: Panoramic radiography can causeincrease the volume of GCF in wistar rats with periodontitis. Keywords: Gingival Crevicular Fluid,panoramic, periodontitis, wistar rats
ANTIBACTERIAL EFFECTIVENESS OF KECAPI SENTUL EXTRACT (SANDORICUM KOETJAPE MERR.) AGAINST STREPTOCOCCUS MUTANS Adhimas Rilo Pambudi; Yusrinie Wasiaturrahmah; Didit Aspriyanto
ODONTO : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.2.1-10

Abstract

ABSTRACTBackground: One of the causing dental caries is a microorganism, namely Streptococcus mutans. Kecapi sentul leaves extract (Sandoricum koetjape Merr.) contain alkaloid, flavonoid, saponin, steroid, phenol, and triterpenoid which have antibacterial properties on the inhibition Streptococcus mutans which has the potential to prevent dental caries.Method: This research uses a laboratory experimental design with a post-test control group only design, using seven treatment groups, namely kecapi sentul leaves extract with the concentration of group 30%, 40%, 50%, 60%, 70%, Chlorhexidine gluconate 0.2% as positive control and aquadest as negative control were repeated 4 times. Result: Non parametric test Kruskal Wallis and Post Hoc Mann Whitney methods showed that each treatment group was significantly different in the diameter of the formed inhibition zone. The mean diameter of the inhibition zone with a concentration of 30% was 9.1 mm, 40% was 13.3 mm, 50% was 17.13 mm, 60% was 18.65 mm and 70% was 21.05 mm.Conclusion: Kecapi sentul leaves extract (Sandoricum koetjape Merr.) with the concentration of group 30%, 40%, 50%, 60% and 70% have antibacterial potential against the growth of Streptococcus mutans.
THE EFFECT OF RED DRAGON FRUIT PEEL EXTRACT ADMINISTRATION IN MICE (Mus musculus) ABSOLUTE MONOCYTE COUNTS IN PERIAPICAL RADIOGRAPHY EXPOSURE Nurlailatul Rahmah; Didit Aspriyanto; R. Harry Dharmawan Setyawardhana
Dentino : Jurnal Kedokteran Gigi Vol 7, No 1 (2022)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v7i1.13098

Abstract

Background: Exposure to periapical radiographic X-ray radiation can indirectly affect biological material which will trigger the formation of free radicals. An imbalance in the level of free radicals causes oxidative stress. To reduce it, additional antioxidants obtained from nature are needed. Natural antioxidants can be obtained from the peel of red dragon fruit which the function is to reduce the presence of free radicals. Objective: to analyze the effect of red dragon fruit peel extract administration in mice absolute monocyte counts in periapical radiographic exposure. Method: This study was a true experimental design with a post-test only with control group design. The research sample used 24 mice. Samples were divided into 6 groups: the control group was not given with the treatment, P1 to P4 group were given red dragon fruit peel extract at a dose of 100 mg / kg bb and exposed to radiation with different exposures, P5 group was given red dragon fruit peel extract but not exposed. P1 group was exposed to 1 dose (0.63 mGy), P2 group was exposed to 4 doses (1.66 mGy), P3 group was exposed to 8 doses (4.37 mGy), and P4 group was exposed to 12 doses (8.19 mGy). Results: This study showed that there were changes in the number of monocytes in all groups. The Games Howell test showed that the P1 group had a significant difference compared to the P4 and P5 groups. Conclusion: There is an effect of red dragon fruit peel extract administration in mice absolute monocyte counts in periapical radiographic exposure.
EFFECTS OF RED DRAGON FRUIT PEEL EXTRACT ADMINISTRATION ON NEUTROPHIL COUNTS FOLLOWING PERIAPICAL RADIOGRAPHY EXPOSURE Maydina Izzatul Yazidah; Didit Aspriyanto; Renie Kumala Dewi
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.11998

Abstract

Background: Radiation exposure from periapical imaging can cause cell damage in various tissues due to the formation of free radicals. Cell damage in the tissue can be observed from the number of neutrophils which are immune cells. Free radicals can be suppressed by administering exogenous antioxidants. One source of natural antioxidants is red dragon fruit, especially on its peel. Objective: To analyze the effect of periapical imaging x-ray radiation exposure after the administration of red dragon fruit peel extract on absolute neutrophil counts in mice. Method: This research was a true experimental study with a post-test only and control group design using 24 mice that were divided into 6 groups. The control group was not given any treatment while P1 to P5 group were given red dragon fruit peel extract at a dose of 100 mg/kg BW and exposed to radiation with different exposures. P1 group was exposed 1 time (0.63 mGy), P2 group was exposed 4 times (1.66 mGy), P3 group was exposed 8 times (4.37 mGy), P4 group was exposed 12 times (8.19 mGy), and group P5 was given no exposure. Results: There were changes in the number of neutrophils in all groups. The LSD Posthoc test results showed a significant difference between the control group (12.470 / mm3) and the P5 group (12.470 / mm3) compared to P3 group with 8 times exposure (40.348 / mm3). Conclusion: There is an effect of periapical radiographic x-ray radiation exposure by red dragon fruit peel extract on the absolute neutrophil count in mice. Keywords: Antioxidants, Neutrophils, Periapical Radiography, Radiation, Red Dragon Fruit Peel.
ANTIBACTERIAL EFFECTIVITY OF KASTURI LEAF EXTRACT (Mangifera casturi) AGAINST THE GROWTH OF Streptococcus sanguinis BACTERIA Dita Puspita Sari; Didit Aspriyanto; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8118

Abstract

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.
THE CHANGE OF SALIVARY pH AFTER RINSING WITH FUJI APPLE (Malus sylvestris) EXTRACT 100% ON CHILDREN AGED 8-10 YEARS. (Review onstudents in SDN Pengambangan 3 Banjarmasin) Dian Dwiyanti; Didit Aspriyanto; Isyana Erlita
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4600

Abstract

Background: The age of 8-10 is the age group with highest number of dmf-t in children. The normal salivary pH can prevent the formation of caries. Fuji apple has contents that are antibacterial such as flavonoid, catechin and tannin that reduce the production of salivary acid by Streptococcus mutanbactery. Purpose: To analyze the difference change of pH saliva before and after rinsing with Fuji apple (Malus sylvestris) extract100% and Chlorhexidine 0,2% in oral cavity of children aged 8-10 years old. Method: This study used quasi experimental method with pre-post test control group design. The samples were determined by purposive random sampling on children aged 8-10 years old in SDN Pengambangan 3 Banjarmasin with the total of 63 people. Samples were divided to 3 treatment groups which are rinsing with Fuji apple (Malus sylvestris) extract 100%, Chlorhexidine 0,2% and aquades. Result: The result of One-way Anova test showed that there was difference on the three treatment groups (p=0,0001). The result of Posthoc Bonferroni test showed that there was significant difference between Fuji apple (Malus sylvestris) extract 100%, Chlorhexidine 0,2% (p=0,016). There was significant difference between Fuji apple (Malus sylvestris) extract 100% and Aquades (p=0,0001) and between Chlorhexidine 0,2% andaquades (p=0,0001). The increase of salivary pH was found in three groups treatment. Conclusion: There is significant difference between groups that rinse with Fuji apple (Malus sylvestris) extract and Chlorhexidine 0,2% in children aged 8-10 years old.
Co-Authors A'idah, Nurul Adhimas Rilo Pambudi Ahda Ahda Annisa Aini, Lisa Shofa’ Nur Aliffia Azizah Kawiadji Alya Royyana Amy Nindia Carabelly Arifin, Rahmad At-Thoyyar, Aila Audina, Nina Aulia Azizah Azaria, Nisa Bayu Indra Sukmana Bayu Yordha Senggara Beta Widya Oktiani Brachmedio Barito Syech Erlangga Debby Saputera, Debby Desty Ayu Dwiyanti Dewi Nurdiana Dewi Puspitasari Dewi Puspitasari Dian Dwiyanti Dita Puspita Sari Diza Afira Hutasuhut Dwi Kurniawan, Fajar Kusuma Dwiyanti, Dian Eny Febriyanti Erika Norfitriyah Firda Damayanti Fitri Siregar, Eka Dwita Natasya Helda Helda Hendrik Setia Budi I Wayan Arya Krishnawan Firdaus Ichrom Nahzi, Muhammad Yanuar Ihsan, Muhammad Fauzan Ika Kusuma Wardani Intan Nirwana, Intan Jatmiko, Habibi Naufal Khairunnisa Puspita Sari Krishnawan Firdaus, I Wayan Arya Maulana, Noval Ihza Maydina Izzatul Yazidah Milka Widya Sari Muhammad Hasanu Reksi Muhammad Yunanda Anhar Nadia Chairina Nahzi, Yanuar Ichrom Naila Khairiyah Nina Audina Nor Rahmatika, Aniqah Norfitriyah, Erika Novi Tiara Lestari Nurlailatul Rahmah Nurrahman, Tri Nurul Fathanah Putri Oktiani, Beta Widya Prilly Sonya Puteri Priyawan Rachmadi Putra Ramadhani, Krisna Erlangga Ramadhaniyah, Nur Ramadhanty, Aina Renie Kumala Dewi Rini Rahmiyati Rosdayatri, Nadhira Agnia Rosihan Adhani, Rosihan Rudie Syahrizal Akhmad Sari, Milka Widya Sarifah, Norlaila Senggara, Bayu Yordha Setyawardhana, R. Harry Dharmawan Setyawardhana, Raden Harry Dharmawan Sherli Diana Syifa Ennisa Tri Putri, Deby Kania US Ramadilla Ramadilla Utami, Juliyatin Putri Wydiamala, Erida Yusrinie Wasiaturrahmah