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Journal : ZOO INDONESIA

EVALUASI METODE SEKSING NURI KEPALA HITAM (Lorius lory LINNAEUS, 1758) DI FASILITAS PENANGKARAN PUSAT PENELITIAN BIOLOGI Nugroho, Herjuno Ari; Zein, Moch. Syamsul Arifin
ZOO INDONESIA Vol 24, No 2 (2015): Desember 2015
Publisher : Masyarakat Zoologi Indonesia

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Abstract

Penentuan jenis kelamin pada burung monomorfik seperti Nuri Kepala Hitam (Lorius lory) kadang sulit dilakukan, meskipun hal itu penting dalam program penangkaran. Tujuan dari studi ini untuk mengevaluasi reliabilitas dan efektifitas metode seksing untuk Nuri Kepala Hitam berdasarkan pengukuran karakter morfologi (morfometri) dan identifikasi molekuler menggunakan gen CHD1 dari kromosom seks. Analisis diskriminan diterapkan pada ukuran panjang tubuh dan culmen dari 9 spesimen museum. Fungsi yang terbentuk diaplikasikan pada 6 ekor burung hidup dan mampu mengklasifikasikan dengan benar 83,33% individu (5/6). Amplifikasi gen CHD1 mampu menentukan 100% sampel (6/6) dengan jantan menunjukkan pita DNA tunggal (ZZ) dan betina menunjukkan pita DNA ganda (ZW). Berdasarkan perbandingan dan evaluasi diantara kedua metode, metode molekular lebih akurat dan aplikatif digunakan untuk determinasi seksual Nuri Kepala Hitam di Fasilitas Penangkaran, Pusat Penelitian Biologi LIPI dibandingkan dengan metode morfometri. Penelitian ini menganjurkan bahwa teknik molekuler dapat digunakan dalam determinasi kelamin burung monomorfik.
A Preliminary Study of Aphrodisiac Property from Porcupine Tail Meat Ethanol Extract in Male Mice Agusta, Andria; Farida, Wartika Rosa; Nugroho, Herjuno Ari; Wulansari, Dewi; Anita, Syahfitri
ZOO INDONESIA Vol 26, No 1 (2017): Juli 2017
Publisher : Masyarakat Zoologi Indonesia

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Abstract

This study aim to examine the aphrodisiac activity of ethanol extract from the tail meat of porcupine (Hystrix javanica F. Cuvier, 1823) that is traditionally believed by people in Java (Indonesia) could enhance male vitality and sexual perfor-mance. Twenty sexually inexperienced male mice were randomly divided into four groups and paired with artificially estrus female mice after one hour of drug an extract administration. Two doses of ethanol extract; 150 and 750 mg/kg were administered (p.o) to male mice. Sildenafil citrate was used as the positive control while 5% Tween 80 solution used as the negative control. Sexual behavior parameters such as mounting and intromission were observed for three hours of mating. Male mice treated with ethanol extract of porcupine tail meat at the dose 750 mg/kg BW showed higher mounting and intromission frequency compared to the group of ethanol extract dose 150 mg/kg. After 2 hours of admin-istration showed the highest frequency compared to all groups. However, the ethanol extract could not reduce the mount-ing and intromission latency as low as sildenafil citrated treated group. Present findings provide preliminary evidence of aphrodisiac properties from the ethanol extract of porcupine tail meat.