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All Journal Universa Medicina International Journal of Cell and Biomedical Science
Mohammad Ariq Nazar
Stem Cell And Cancer Research Laboratory, Semarang, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Hepatoprotective activity of Averrhoa bilimbi L. fruit extract on carbon tetrachloride-induced acute liver faiure in Wistar rats Rachellicya Ristioni Sihaloho; Chodijah Chodijah; Titiek Sumarawati; Joko Wahyu Wibowo; Siti Thomas Zulaikhah; Agung Putra; Nurul Hidayah; Shabrina Syifa Ghaissani; Mohammad Ariq Nazar
Universa Medicina Vol. 42 No. 1 (2023)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2023.v42.61-69

Abstract

Background Acute liver failure (ALF) is a state of rapid and progressive deterioration of liver function. Continuous exposure to chemicals and viruses can increase reactive oxygen species (ROS) which leads to prolonged inflammation due to the production of tumor necrosis factor-alpha (TNF-á) thus inhibiting the production of platelet-derived growth factor (PDGF). The objective of this study was to evaluate the effect of administration of Averrhoa bilimbi L. fruit extract on PDGF levels and TNF-á levels in carbon tetrachloride (CCl4)-induced ALF rats. MethodsThis study used a post-test-only control group design involving 20 Wistar rats. They were randomized into 4 groups, namely sham, control, T1, and T2. Group T1 was exposed to CCl4 with the administration of A. bilimbi fruit extract at a dose of 500mg/kgBW, while, group T2 was exposed to CCl4 and given A. bilimbi fruit extract of 750 mg/kgBW. On the 15th day, the serum was analyzed to determine the levels of PDGF and TNF-á using ELISA. ResultsThe highest mean PDGF level in the control group was 146.60±15.36 pg/mL, while the highest mean TNF-á level in group T1 was 40.11±4.44 pg/mL. The One-way ANOVA test showed that there were significant differences in TNF-á (p=0.002) and PDGF (p=0.000) levels between the study groups. ConclusionThe administration of A.bilimbi L. fruit extract affected PDGF and TNF-á levels in CCl4-induced ALF rats. The present study revealed that A. bilimbi fruits have significant hepatoprotective activity in experimental Wistar rats.
Downregulation of IL-6 and TNF-α Expression with Mesenchymal Stem Cells Therapy in Allergic Rhinitis Rats Models Restimulia, Lia; Dewi, Dian Andriani Ratna; Nazar, Mohammad Ariq; Irawan, Risky Chandra Satria; Ghaissani, Shabrina Syifa; Haryono, Erlina
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.13

Abstract

Rhinitis is an inflammatory that characterized by nasal symptoms due to the condition of the nasal mucosa that trigerred by an interaction between environmental allergens and immunoglobulin (Ig)E. It is driven by host factors, infection, pathogens and various inflammatory pathways such as TNF-α that released in allergic responses from both mast cells and macrophages through IgE-dependent mechanisms. Secretome hypoxia mesenchymal stem cells (SH-MSCs) contain anti-inflammatory soluble molecules were able to improve the conditions of endothelial damage, inflammation and oxidative stress by decreasing levels of IL-6, TNF-α, ROS and increasing eNOS. This study will investigate the effect of SH-MSCs at a dose of 300 µL on TNF- and IL-6 expression in rat model ovalbumin-induced rhinitis in vivo. Thirty male Wistar rats were randomly divided into 3 groups: control, treatment and sham group that administrated through intramuscular injection. The results of this study found that SH-MSCs can downregulate the inflammatory cytokines TNF-α and IL-6 in rat model ovalbumin-induced rhinitis in vivo
Regulatory Effect of Secretome-Hypoxia Preconditioned Mesenchymal Stem Cells on TNF-α Level in Streptozotocin-induced Rats Amellia, Nazzala Luthfin; Antari, Arini Dewi; Nazar, Mohammad Ariq; Husain, Sofian Azalia; Arifin, Naufal Ardjivani
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.23

Abstract

Background: Type 1 Diabetic Mellitus (T1DM) is a well-known autoimmune disease characterized by a specific adaptative immunity against β-cell antigens. Mesenchymal stem cells (MSC) have emerged as potential immunomodulators in a paracrine manner via their bioactive soluble molecules that involve inflammation-related diseases, including T1DM. Objective: This study aims to investigate the effect of SHMSCs on regulating TNF-α concentrations in STZ-induced rats. Materials and Methods: This study uses a post-only control group design and randomized system that was conducted from December 2022 until January 2023. To induce T1DM-like rats, an intraperitoneal injection (65 mg/kg BW) of streptozotocin (STZ) was inducted. 15 male Wistar rats were subdivided into the following groups: STZ, STZ with 0.5 cc SHMSCs (Low-dose), and STZ with 1 cc SHMSCs (High-dose). The animals received an intraperitoneal injection of SHMSCs once a week for up to 4 weeks. On day 28, the animals were terminated and ELISA measured TNF-α concentrations. Results: After SHMSCs administration, the level of TNF-α in the treated group was decreased in either low-dose or high-dose groups compared with the STZ group. Conclusion: Administration of secretome-hypoxia MSCs may regulate TNF-α concentrations in STZ-induced Rats.
Effect of Typhonium flagelliforme Extract on the Viability of Colorectal Cancer Cells HCT-116 Sadikin, Nadya Audina N; Nazar, M. Ariq; Ibrahim, Sugeng
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.46

Abstract

Background: Colorectal cancer (CRC) remains a significant global health challenge, with rising incidence rates, particularly among younger individuals [1]. Conventional treatments, including chemotherapy and radiation, often cause severe side effects, necessitating the exploration of alternative therapeutic options. Typhonium flagelliforme, a medicinal plant widely used in traditional medicine, has been investigated for its cytotoxic potential against HCT-116 colorectal cancer cells. Objective: This study assessed the cytotoxic effects of T. flagelliforme extract on HCT-116 colorectal cancer cells using the MTT assay. Methods: The cytotoxicity of Typhonium flagelliforme (T. flagelliforme ) extract on HCT-116 colorectal cancer cells was assessed using the MTT assay after 24 hours of treatment with different concentrations (20–100 µg/mL). Results: The findings revealed a dose-dependent reduction in cell viability, with an IC₅₀ value of 73.47 µg/mL, indicating moderate cytotoxic activity. Higher extract concentrations (100–60 µg/mL) significantly decreased cell viability, while the lowest concentration (20 µg/mL) showed a paradoxical increase, possibly due to a hormesis effect. Conclusion: The findings revealed a dose-dependent reduction in cell viability, with an IC₅₀ value of 73.47 µg/mL, indicating moderate cytotoxic activity.
Harnessing the Innate Effector: A Narrative Review of Advancing Strategies in CAR Engineering, Metabolic Reprogramming, and TME Resistance of Natural Killer Cells for Cancer Immunotherapy Agustina, Rita; Lestari, Endah Agustina; Sidiq, Husni Ahmad; Nazar, M. Ariq
International Journal of Cell and Biomedical Science Vol 4 No 11 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i11.70

Abstract

Natural Killer (NK) cell-based immunotherapy is rapidly emerging as a promising modality for cancer treatment. As pivotal players in the innate immune system, cells independently recognize and eliminate malignant cells without prior sensitization, offering distinct advantages over other cell-based therapies. This review highlights the current landscapes of NK cell adoptive therapy, from fundamental biology to cutting-edge clinical applications. It highlights how advancing NK cell sources, including peripheral blood, umbilical cord blood, established cell lines, and the increasingly significant induced pluripotent stem cells (iPSCs), are driving wider, more standardized therapeutic use. The multifaceted strategies employed to enhance NK cell efficacy are being explored, including advanced expansion protocols and sophisticated genetic engineering techniques such as the introduction of Chimeric Antigen Receptors (CARs) and modifications to bolster antibody-dependent cellular cytotoxicity. Additionally, it also addresses the significant hurdles that remain, primarily the immunosuppressive tumor microenvironment (TME), and discusses innovative strategies being developed to overcome these challenges. By synthesizing preclinical data and results from the latest clinical trials, this review highlights the remarkable progress and bright future of NK cell therapy as a safer, effective, and more accessible cornerstone of cancer treatment.
Co-Authors Agung Putra Agustina, Rita Amellia, Nazzala Luthfin Antari, Arini Dewi Arifin, Naufal Ardjivani Chodijah Chodijah Dewi, Dian Andriani Ratna Ghaissani, Shabrina Syifa Haryono, Erlina Husain, Sofian Azalia Ibrahim, Sugeng Irawan, Risky Chandra Satria Joko Wahyu Wibowo Lestari, Endah Agustina Nurul Hidayah Rachellicya Ristioni Sihaloho Restimulia, Lia Sadikin, Nadya Audina N Shabrina Syifa Ghaissani Sidiq, Husni Ahmad Siti Thomas Zulaikhah Titiek Sumarawati