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All Journal JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Jurnal Farmasi Sains dan Komunitas Riset Informasi Kesehatan Media Farmasi
Riesta Primaharinastiti, Riesta
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Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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A Comparative Study of Randu Honey Antimicrobial Activity from Several Regions in Java Nindya Pramesti Wardani; Primaharinastiti, Riesta; Achmad Toto Poernomo; Alfi Khatib
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 11 No. 2 (2024): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v11i22024.147-155

Abstract

Background: Randu honey is monofloral honey sourced from a type of plant nectar. The geographical location of randu (Ceiba pentandra) as the source of nectar is one factor that influences the antimicrobial activity of random honey. This research used randu honey from several regions in Java such as Sidoarjo (RSH), Pusat Perlebahan Nasional Bogor (RBH), Kediri (RKH), and Malang (RMH). Objective: To compare the antimicrobial activity of several random honeys (RSH,RBH,RKH, and RMH) against Gram-negative Escherichia coli ATCC 25922, Gram-positive Staphylococcus aureus ATCC 6538, methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33592, and Candida albicans ATCC 10231. Methods: This study used well diffusion and dilution antimicrobial test methods. The diameter of the inhibition zone formed by the well diffusion method was measured using a Vernier caliper. The diffusion method was used as a screening test before determining the quantitative minimum inhibitory concentration (MIC) using serial dilution at a ratio1 of 2 (v/v). Streptomycin and Ketoconazole were used as positive controls. Nutrient broth and Sabouraud broth were incubated at 37°C for 24 h (antibacterial tests) and 25°C for 48 h (antifungal test), respectively. Results: The well diffusion test revealed that all random honey samples could inhibit the test bacteria and fungi with the appearance of an inhibition zone. Diameter inhibition zone ranged from 14.66±0.52 mm to 27.86±0.43 mm. The MICs of RSH,RBH,RKH, and RMH ranged from 3.12% to 25% against all test bacteria and fungi. Conclusion: The results of this study showed randu honey from Bogor (RBH) has the highest antimicrobial activity based on diffusion and dilution tests.
Teknik Aplikasi Sampel Pada Pengujian Kuantitatif Kromatografi Lapis Tipis: Tinjauan Terhadap Area Dan Faktor Retensi Kusumawati, Idha; Primaharinastiti, Riesta; Rifqi Prasetyawan, Hanif
Media Farmasi Vol 20 No 2 (2024): Media Farmasi Edisi Oktober 2024
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v20i2.655

Abstract

Thin Layer Chromatography (TLC) is one of the most commonly used chromatographic techniques to identify compounds in mixtures and determine the purity of a compound. However, the crucial factor that is difficult to control, which can affect the accuracy of the results, is the stage of sample application. This study focused on investigating the effect of different techniques of sample application (manual and semi-automatic) on the area and retention factor (Rf) of the compound spot. Curcuminoid was used as a sample in this study. The manual sample application techniques used a 2μL capillary tube. The semi-automatic sample application techniques used a Camag Linomat-5 instrument with 0, 7, and 8 mm band lengths. After the development of the TLC plate using toluene: acetic acid (4:1, v/v)., the curcuminoid was separated into three spots: curcumin, demethoxycurcumin, and bisdemethoxycurcumin. A one-way ANOVA was used to analyze the spot area and Rf of the three compounds, followed by a Duncan test. The difference in sample application techniques causes differences in the size and shape of the initial sample spot, which affects the compound spot produced after developing of TLC plate. This study showed that the differences in application techniques cause significant differences in the Rf of the compound spot, but do not show significant differences in the compound spot area. Based on this research, optimizing the application techniques of samples is a critical stage and it is essential to optimize how to apply the sample on TLC plate, including the concentration, volume, shape, and size of the spot on TLC.
Antibacterial activity of Randu Honey against some bacterial pathogens using agar well diffusion method Megawati, Megawati; Yuwono, Mochammad; Primaharinastiti, Riesta
Riset Informasi Kesehatan Vol 14 No 1 (2025): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v14i1.954

Abstract

Background: Honey's antibacterial and other therapeutic qualities have long been recognized because of its bioactive components, which include hydrogen peroxide, flavonoids, and phenolics. Randu honey is a monofloral honey that is made from the nectar of Ceiba pentandra and has the ability to suppress Gram-positive as well as Gram-negative bacteria. Because antibiotic resistance is on the rise, it is crucial to investigate alternative treatments like honey. Method: This study evaluates the antibacterial activity of randu honey using the agar well diffusion method or Kirby-Bauer diffusion method against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa to determine its Minimum Inhibitory Concentration (MIC) by preparing a series of honey dilutions ranging from 100% to 10% (w/v) then incubating in an incubator at a temperature of 37°C for 24 hours to allow interaction between the test solutions and bacteria on the agar media, followed by measuring the inhibition zones formed using a caliper. Results: The antibacterial activity of randu honey increased with concentration. Staphylococcus aureus showed the highest sensitivity with an inhibition zone of 9.02 ± 0.67 mm at 100%, classified as moderate at 70%–100% concentrations (MIC 20%). Escherichia coli exhibited lower sensitivity, with inhibition zones ranging from 7.07 ± 0.56 mm at 100% to 0.18 ± 0.01 mm at 30%, categorized as moderate at 80%–100% (MIC 30%). For Pseudomonas aeruginosa, moderate activity was observed at 80%–100%, with inhibition zones up to 8.16 ± 0.13 mm at 100% (MIC 30%). Conclusion: Randu honey shows promising antibacterial activity, especially against Staphylococcus aureus, with concentration-dependent effects. Its moderate activity at higher concentrations highlights its potential as a natural antibacterial agent.
Development of Chemometric Methods for Determining Caffeine Content in Robusta Coffee as Alternative Standardization Techniques Handoyo, Theodorus Rexa; Primaharinastiti, Riesta; Yuwono, Mochammad
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 22, No 2 (2025)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.009242

Abstract

There is a necessity for standardization to uphold coffee quality due to its frequent production and consumption. High-performance liquid chromatography (HPLC) serves as the benchmark for caffeine analysis in coffee in Indonesia. However, its high cost has prompted the exploration of alternative methods. This study aims to obtain a suitable alternative method for determining the caffeine content in robusta coffee as an effort toward standardization. Employing statistical and mathematical techniques in chemistry, chemometrics emerges as a promising alternative in analyzing caffeine in coffee. The approach was integrated with an ultraviolet (UV) spectrophotometer capable of analyzing substances across wavelengths from 200 to 400 nm. From the five replications, the HPLC method showed a caffeine content of 1.435 ± 0.011% (w/w), while the spectrophotometer showed a content of 1.723 ± 0.003% (w/w). Through the partial least squares regression (PLSR) and principal component regression (PCR) methods in RStudio, the results obtained were 1.432 ± 0.003% and 1.430 ± 0.002% (w/w), respectively. These results indicate good REP values of 0.022% for PLSR and 0.155% for PCR. With these findings, it is hoped that chemometrics can become an alternative method for analyzing caffeine in coffee.
Co-Authors Achmad Toto Poernomo Alfi Khatib Idha Kusumawati Megawati - Mochammad Yuwono Nindya Pramesti Wardani Rifqi Prasetyawan, Hanif Theodorus Rexa Handoyo