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Effects of Nitrification Inhibitors on Mineral Nitrogen Dynamics in Agriculture Soils Tindaon, Ferisman; Benckiser, Gero; Ottow, Johannes Carl Gottlieb
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Experiments were conducted under laboratory conditions to elucidate the effect of three nitrification inhibitors viz, 3.4dime-thylpyrazo-lephosphate (DMPP), 4-Chlormethylpyrazole (ClMP) and dicyandiamide (DCD) on mineral nitrogen dynamics of (NH4)2SO4 in soil incubated at 25oC in soils. The quantitative determination of ammonium, nitrite and nitrate were carried out spectrophotometrically, while potential denitrify-cation capacity (PDC) was measured gas chromatographically. DMPP, ClMP and DCD were used on recommended rates of 90kg N ha-1 corres-ponding to 0.36µg DMPP; 0.25µg ClMP and 10µg DCD g-1 dry soil. In all treatments, the influence of 1, 10, 50, 100, 250 and 500 times of the recommended-concentrations were examined. Results suggested that DMPP, ClMP and DCD applied at rates generally recommended for agricultural use may not be effective to inhibit nitrification. Thus even at the highest tested NIs-concentrations, nitrate and nitrite formation still occurred. Application of high concentrations of these chemicals up to 180µg DMPP, 125µg ClMP and 2500µg DCD were needed for inhibiting nitrification completely. The three NIs began to inhibit PDC at 10 to 50 times recommended concentration and were more effective in sandy than in loamy or clay soils. ClMP influenced PDC at much lower concentration as DMPP or DCD.   Keywords: nitrification inhibitors, mineral nitrogen dynamics
Effects of Nitrification Inhibitors on Mineral Nitrogen Dynamics in Agriculture Soils Tindaon, Ferisman; Benckiser, Gero; Ottow, Johannes Carl Gottlieb
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.86

Abstract

Experiments were conducted under laboratory conditions to elucidate the effect of three nitrification inhibitors viz, 3.4dime-thylpyrazo-lephosphate (DMPP), 4-Chlormethylpyrazole (ClMP) and dicyandiamide (DCD) on mineral nitrogen dynamics of (NH4)2SO4 in soil incubated at 25oC in soils. The quantitative determination of ammonium, nitrite and nitrate were carried out spectrophotometrically, while potential denitrify-cation capacity (PDC) was measured gas chromatographically. DMPP, ClMP and DCD were used on recommended rates of 90kg N ha-1 corres-ponding to 0.36µg DMPP; 0.25µg ClMP and 10µg DCD g-1 dry soil. In all treatments, the influence of 1, 10, 50, 100, 250 and 500 times of the recommended-concentrations were examined. Results suggested that DMPP, ClMP and DCD applied at rates generally recommended for agricultural use may not be effective to inhibit nitrification. Thus even at the highest tested NIs-concentrations, nitrate and nitrite formation still occurred. Application of high concentrations of these chemicals up to 180µg DMPP, 125µg ClMP and 2500µg DCD were needed for inhibiting nitrification completely. The three NIs began to inhibit PDC at 10 to 50 times recommended concentration and were more effective in sandy than in loamy or clay soils. ClMP influenced PDC at much lower concentration as DMPP or DCD.   Keywords: nitrification inhibitors, mineral nitrogen dynamics
PENGARUH INHIBITOR NITRIFIKASI TERHADAP AKTIVITAS DENITRIFIKASI OLEH AZOSPIRILLUM BRASILENSE DAN SINORHIZOBIUM SP Ferisman Tindaon -; Tualar Simarmata -
Bionatura Vol 13, No 3 (2011): Bionatura Nopember 2011
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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Abstract

Kajian pengaruh inhibitor nitrifikasi 3,4-dimethylpyrazolephosphat (DMPP) terhadap denitrifikasi dan produksi N2O/N2 oleh bakteri pemfiksasi nitrogen (Azospirillum brasilense Az204 and Sinorhizobium sp TNAU14) telah dilakukan dalam bentuk percobaan model pada kondisi laboratorium. Percobaan terdiri dari 3 perlakuan: kontrol: perlakuan pemberian 1% v/v C2H2 dan pemberian 1 μg DMPP mL-1 yang dikombinasikan dengan inokulasi Azospirillum brasilense atau Sinorhizobium sp. Pengukuran dilakukan terhadap konsentrasi gas yang dihasilkan yaitu N2O dan CO2, kerapatan populasi, kandungan nitrat, nitrit, dan ammonium dalam larutan media. Hasil penelitian menunjukkan bahwa produksi N2O oleh Azospirillum brasilense dengan perlakuan dan tanpa perlakuan 1 % asetylen dihasilkan masing-masing sekitar 2–18 μg N2O mL-1hari-1, dan 2–17 μg N2O mL-1 hari-1. Produksi N2O oleh Sinorhizobium sp berkisar 4–15 μg N2O mL-1 hari-1 dan 4–12 μg N2O mL-1 hari-1 yaitu dengan pemberian dan tanpa pemberian 1% asetylen. Kehilangan nitrat dalam bentuk emisi N2O melalui denitrifikasi oleh Azosprillum brasilense atau Sinorhizobium sp berkisar 70–98% dan 80–95% dan menurun hingga sekitar 50–51% akibat penurunan suplai nitrat. Perlakuan 1 μg DMPP mL-1 pada media pertumbuhan dapat menurunkan jumlah populasi kedua bakteri pemfiksasi nitrogen. Produksi CO2 oleh Azospirilum brasilense menurun secara signifikan. DMPP dapat mempengaruhi populasi mikroorganisma non target di dalam tanah.Kata kunci : Azosprillium brasilense, Sinorhizobium sp, inhibitor nitrifikasi
PENGARUH INHIBITOR NITRIFIKASI TERHADAP AKTIVITAS DENITRIFIKASI OLEH AZOSPIRILLUM BRASILENSE DAN SINORHIZOBIUM SP Ferisman Tindaon -; Tualar Simarmata -
Bionatura Vol 13, No 3 (2011): Bionatura Nopember 2011
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kajian pengaruh inhibitor nitrifikasi 3,4-dimethylpyrazolephosphat (DMPP) terhadap denitrifikasi dan produksi N2O/N2 oleh bakteri pemfiksasi nitrogen (Azospirillum brasilense Az204 and Sinorhizobium sp TNAU14) telah dilakukan dalam bentuk percobaan model pada kondisi laboratorium. Percobaan terdiri dari 3 perlakuan: kontrol: perlakuan pemberian 1% v/v C2H2 dan pemberian 1 μg DMPP mL-1 yang dikombinasikan dengan inokulasi Azospirillum brasilense atau Sinorhizobium sp. Pengukuran dilakukan terhadap konsentrasi gas yang dihasilkan yaitu N2O dan CO2, kerapatan populasi, kandungan nitrat, nitrit, dan ammonium dalam larutan media. Hasil penelitian menunjukkan bahwa produksi N2O oleh Azospirillum brasilense dengan perlakuan dan tanpa perlakuan 1 % asetylen dihasilkan masing-masing sekitar 2–18 μg N2O mL-1hari-1, dan 2–17 μg N2O mL-1 hari-1. Produksi N2O oleh Sinorhizobium sp berkisar 4–15 μg N2O mL-1 hari-1 dan 4–12 μg N2O mL-1 hari-1 yaitu dengan pemberian dan tanpa pemberian 1% asetylen. Kehilangan nitrat dalam bentuk emisi N2O melalui denitrifikasi oleh Azosprillum brasilense atau Sinorhizobium sp berkisar 70–98% dan 80–95% dan menurun hingga sekitar 50–51% akibat penurunan suplai nitrat. Perlakuan 1 μg DMPP mL-1 pada media pertumbuhan dapat menurunkan jumlah populasi kedua bakteri pemfiksasi nitrogen. Produksi CO2 oleh Azospirilum brasilense menurun secara signifikan. DMPP dapat mempengaruhi populasi mikroorganisma non target di dalam tanah.Kata kunci : Azosprillium brasilense, Sinorhizobium sp, inhibitor nitrifikasi
Side Effects of Nitrification Inhibitors on Non Target Microbial Processes in Soils Ferisman Tindaon; Gero Benckiser; Carl Gottlieb Ottow
JOURNAL OF TROPICAL SOILS Vol 16, No 1: January 2011
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2011.v16i1.7-16

Abstract

Agricultural chemicals have been used extensively in modern agriculture and toxicological studies suggest a great potential for inducing undesirable effects on non target organisms. A model experiment was conducted in order to determine side effects of three nitrification inhibitors (NIs, 3,4dimethylpyrazolephosphate = DMPP, 4-Chlormethylpyrazole phosphate = ClMPP and dicyandiamide = DCD) on non target microbial processes in soils. Side effects and dose response curve of three NIs were quantified under laboratory conditions using silty clay, loam anda sandy soils. Dehydrogenase, dimethylsulfoxide reductase as well as nitrogenase activity (NA) and potential denitrification capacity were measured as common and specific non target microbial processes. The influence of 5-1000 times the base concentration, dose response curves were examined, and no observable effect level = NOEL, as well as effective dose ED10 and ED50 (10% and 50% inhibition) were calculated. The NOEL for microbial non target processes were about 30–70 times higher than base concentration in all investigated soils. The potential denitrification capacity revealed to be the most sensitive parameter. ClMPP exhibited the strongest influence on the non target microbial processes in the three soils. The NOEL, ED10 and ED50 values were higher in clay than in loamy or sandy soil. The NIs was the most effective in sandy soils.Keywords: microbial non target processes, nitrification inhibitors, soil enzymes
Side Effects of Nitrification Inhibitors on Non Target Microbial Processes in Soils Ferisman Tindaon; Gero Benckiser; Carl Gottlieb Ottow
JOURNAL OF TROPICAL SOILS Vol 16, No 1: January 2011
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2011.v16i1.7-16

Abstract

Agricultural chemicals have been used extensively in modern agriculture and toxicological studies suggest a great potential for inducing undesirable effects on non target organisms. A model experiment was conducted in order to determine side effects of three nitrification inhibitors (NIs, 3,4dimethylpyrazolephosphate = DMPP, 4-Chlormethylpyrazole phosphate = ClMPP and dicyandiamide = DCD) on non target microbial processes in soils. Side effects and dose response curve of three NIs were quantified under laboratory conditions using silty clay, loam anda sandy soils. Dehydrogenase, dimethylsulfoxide reductase as well as nitrogenase activity (NA) and potential denitrification capacity were measured as common and specific non target microbial processes. The influence of 5-1000 times the base concentration, dose response curves were examined, and no observable effect level = NOEL, as well as effective dose ED10 and ED50 (10% and 50% inhibition) were calculated. The NOEL for microbial non target processes were about 30–70 times higher than base concentration in all investigated soils. The potential denitrification capacity revealed to be the most sensitive parameter. ClMPP exhibited the strongest influence on the non target microbial processes in the three soils. The NOEL, ED10 and ED50 values were higher in clay than in loamy or sandy soil. The NIs was the most effective in sandy soils.Keywords: microbial non target processes, nitrification inhibitors, soil enzymes
PEMBUATAN PUPUK ORGANIK CAIR (POC) DARI LIMBAH RUMAH TANGGA UNTUK TANAMAN DI PEKARANGAN Susana Tabah T.Panjaitan; Ferlist R. Siahaan; Hotden Leonardo Nainggolan; Parlindungan Lumbanraja; Ferisman Tindaon
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 5, No 2 (2022): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v5i2.530-539

Abstract

Kegiatan penyuluhan atau pengabdian kepada masyarakat ini dilaksanakan pada bulan September 2021, di Gereja HKBP Nommensen, Resort Pulu Brayan, Kecamatan Medan Timur Kota Medan. Kegiatan pengabdian ini diikuti 20 orang ibu-ibu dari komunitas parompuan (komunitas wanita Gereja) HKBP Pulu Brayan sebagai partisipan. Kegiatan pengabdian  dilakukan dengan metode; pemberdayaan partisipatif melalui penyuluhan, diskusi dan diakhiri dengan pelaksanaan evaluasi. Berdasarkan penyuluhan dapat disimpulkan terjadi peningkatan pemahaman komunitas parompuan (komunitas wanita Gereja) HKBP Pulu Brayan Resort Pulu Brayan, Kota Medan sebagai partisipan terkait dengan pembuatan pupuk organik cair dari limbah rumah tangga setelah mengikuti ceramah (penyuluhan). 
Aplikasi Pupuk Kandang dan Mikoriza terhadap Peningkatan P-tersedia, serapan P serta Pertumbuhan dan Produksi Jagung Manis (Zea mays Saccharata L.) Pada Tanah Ultisol Parlindungan Lumbanraja; Bangun Tampubolon; Samse Pandiangan; Johan Ambarita; Ferisman Tindaon
AGRIUM: Jurnal Ilmu Pertanian Vol 26, No 1 (2023)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v26i1.13806

Abstract

AbstractKondisi tanah ultisol yang kurang subur memerlukan penanganan yang kompleks agar dapat berfungsi dengan baik sebagai lahan pertanian. Upaya tersebut diharapkan mampu memberi perbaikan kesuburan tanah secara menyeluruh.  Untuk tujuan ini dilalukan suatu penelitian yang menggunakan pupuk kandang dan pupuk hayati mikoriza yang diharapakan dapat memperbaiki sifat-sifat tanah tersebut hingga diperoleh tingkat pengaruh aplikasi bahan tersebut pada tingkat yang optimal. Penelitian dilakukan dengan Rancangan Acak Kelompok, dengan dua factor.  Faktor pupuk kandang 3 taraf dan factor mikoriza 4 taraf. Setiap pengaruh parameter yang signifikan akan dilanjutkan dengan uji jarak berganda Duncan. Untuk pengamatan dilakukan pengukuran tanah tersedia fosfor, kandungan fosfor tongkol jagung, dan produksi jagung manis. Produksi jagung manis tertinggi 16,06 t/ha terjadi dengan kombinasi perlakuan pada aplikasi atau pemberian pupuk kandang setara dengan 5 t/ha dan mikoriza 3 g/lubang tanam, produksi ini lebih tinggi 3,83 t/ha setara dengan 31,31 % dibandingkan dengan kontrol. Pupuk kandang hanya meningkatkan kandungan fosfor secara nyata pada tongkol jagung. Mikoriza hanya meningkatkan ketersediaan fosfor tanah secara signifikan.