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All Journal Planta Tropika PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat BIO-EDU: Jurnal Pendidikan Biologi Tropical Plantation Journal
Handayani, Etty
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Muhammadiyah Yogyakarta, Jl. Lingkar Selatan, Kasihan, Bantul, Yogyakarta 55183, Indonesia Telp. 0274 387656
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Education Law, Crime, Criminology & Criminal Justice Public Health Social Sciences

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Journal : Tropical Plantation Journal

 1 
The Effect of Foliar Fertilizers as Substitution Media on The Growth of Chrysanthemum morifolium Shoot by In Vitro Culture Handayani, Etty
Tropical Plantation Journal Vol 4, No 1 (2025): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v4i1.50

Abstract

The production of chrysanthemum plants in large quantities can be obtained by in vitro growth of chrysanthemums. The growth media that is often used is MS media which has a relatively high price. Therefore, a substitution medium that has a relatively affordable price is needed in the form of foliar fertilizer media with coconut water and ambon bananas added. This study aims to examine the best medium that can be used for in vitro chrysanthemum growth. The research was carried out in June-September 2020 at the In Vitro Culture Laboratory, Faculty of Agriculture, University of Muhammadiyah Yogyakarta. The research was carried out by a single-factor experimental method with 7 treatments and each had 5 replicates and each replicate consisted of 3 samples arranged with a Complete Random Design (RAL). The treatment that was experimented with was the use of foliar fertilizers in the form of Growmore and Hyponex Green with the addition of coconut water and bananas. The concentrations of Growmore foliar fertilizer given were 3 g/l, 4 g/l, and 5 g/l. The concentrations of Green Hyponex foliar fertilizer given were 3 g/l, 4 g/l, and 5 g/l. The results of the research showed that foliar fertilizer added with coconut water and banana ambon could be used as a substitution medium for MS media in chrysanthemum growth in vitro. The use of Growmore 4 g/l foliar fertilizer shows the best hail against the tall growth of chrysanthemum shoots in vitro
The Effect of Sample Weigh, Incubation Duration And Isolation Methods on The Quality of Phalaeonopsis amabilis root DNA Handayani, Etty; Rineksane, Innaka Ageng; Isnawan, Bambang Heri; Setiawan, Haris Anggita; Manto, Agus
Tropical Plantation Journal Vol 4, No 2 (2025): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v4i2.63

Abstract

Orchid Ph. amabilis is one of the plants designated as "Indonesian National Flower" and designated as "Puspa Pesona Indonesia". Orchid Ph. amabilis has a diversity that is not widely known, so that it is necessary to identify it molecularly. DNA isolation is the first step in molecular testing. This study aims to determine the sample weight, incubation time and the appropriate isolation method for DNA isolation from samples of Ph.amabilis orchid roots. In this study, the DNA isolation stage used two experimental methods, namely the CTAB method (Murray and Thompson) and the mini kit. The research method used was a single factor experiment with a combination of sample weight and incubation time consisting of 6 treatments with 3 replications, so 18 experimental units were obtained. The experimental treatment used was from the roots of the orchid Ph. amabilis which has a sample weight of 0.25 g, 0.5 g, and 0.75 g and each sample weight treatment was incubated for 30 minutes and 60 minutes. In addition, DNA isolation using the kit method had a sample weight of 0.25 g, 0.5 g and 0.75 g and an incubation period of 10 minutes. The results showed that the DNA isolation of Ph. amabilis with a sample weight of 0.75g, incubation time of 30 minutes using the CTAB method showed the best treatment in producing DNA with a concentration of 384 ng/µl and a purity of 1.9. This study is expected to provide information on sample weight, incubation time and the appropriate method for DNA isolation of Ph. amabilis orchid roots.
Substitution Media by Utilizing Banana Peel for In Vitro Culture of Chrysanthemum morifolium Handayani, Etty; Rineksane, Innaka Ageng; Sari, Supriyatin Kartika; Permatasari, Fany
Tropical Plantation Journal Vol 5, No 1 (2026): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v5i1.68

Abstract

The use of organic matter in the in vitro culture medium can reduce costs for micropropagation and multiplication of Chrysanthemum in mass production. This study aims to examine the use of banana peel waste in the in vitro culture of Chrysanthemum. The research used an experimental method with single factor and eight treatments with 3 samples and 3 replications. The treatments were: (1) MS medium +  BAP 1 ppm + NAA 0.5 ppm, (2) Foliar fertilizer 3 g/L +  coconut water 150 mL/L, (3) banana endocarp puree 50 g/L, (4)  banana endocarp puree 100 g/L, (5)  banana exocarp puree 50 g/L, (6) banana exocarp puree 100 g/L, (7) banana peel puree 100 g/L, and (8) banana peel puree 100 g/L. All banana peel puree treatments were combined with foliar fertilizer 3g/L + coconut water 150 mL/L. The explant used a single nodus of Chrysanthemum with one leaf resulting from the previous in vitro culture. This study used Chrysanthemum varieties Naweswari and Tadasita from Ornamental Plants Research Center, West Java, Indonesia. The results showed that foliar fertilizer medium added by banana peel puree and coconut water has the good potential to be used as a substitution medium in the in vitro culture of Chrysanthemum varieties Naweswari and Tadasita. The banana peel puree  50 g/L in the foliar fertilizer medium shows the best growth in Chrysanthemum in vitro culture in both Naweswari and Tadasita varieties.
Co-Authors Aji, Seto Purnomo Alawiyah, Riffa Leshia Muhvi Nur Aris, Irfan Ayuningtias, Nandini Bambang Heri Isnawan Budi Kriswanto, Budi Innaka Ageng Rineksane Irsyadi, Muhammad Burhanuddin Khozin, Mohammad Nur Manto, Agus Mochammad Wildan Jadmiko Permatasari, Fany Prayoga, Mohammad Candra Rani Agustina Wulandari, Rani Agustina Ratnasari, Tri Restanto, Didik Pudji Sari, Supriyatin Kartika Setiawan, Haris Anggita