Handayani, Etty
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Muhammadiyah Yogyakarta, Jl. Lingkar Selatan, Kasihan, Bantul, Yogyakarta 55183, Indonesia Telp. 0274 387656

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Journal : Planta Tropika

Substitusi Medium Sintetik dengan Pupuk Daun, Air Kelapa dan Ekstrak Nabati pada Subkultur Anggrek Cattleya pastoral Innocence secara In Vitro Handayani, Etty; Isnawan, Bambang Heri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 2, No 2 (2014)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2014.031.115-124

Abstract

A research was perfomed to find the proper of coconut water and natural ekstracts combined with Hyponex medium that compared with Vacin & Went medium. This research has been done at Tissue Culture Laboratory, Agricultural Faculty, Universitas Muhammadiyah Yogyakarta. The method of this research was arranged in Randomized Complete Design with 9 treatments and 8 replications. The treatments were VW + NAA 0,5 ppm + BAP 3 ppm, green Hyponex 3 g/l, green Hyponex 3 g/l + bean sprouts 150 g/l, green Hyponex 3 g/l + tomatoes 150 ml/l, green Hyponex 3 g/l + avocado 150 g/l, red Hyponex 3 g/l, red Hyponex 3 g/l + bean sprouts 150 g/l, red Hyponex 3 g/l + tomatoes 150 ml/l, red Hyponex 3 g/l + avocado 150 g/l. The each of Hyponex mediums were given with bananas 150 g/l and coconut water 150 ml/l. The result of this research showed that Hyponex mediums combined with natural ekstract was not signicicantly with Vacin & Went medium to high and shoot growth. Red Hyponex medium 3 g/l combined bananas 150 g/l and coconut water 150 ml/l resulted the best high, leaf and shoot in tissue culture Cattleya pastoral innocence orchid.
The Effect of Benzyl Amino Purin and NaphtalenaAcetic Acid Applications on Direct Shoot Organogenesis in Porang (Amorphophallus muelleri B) Restanto, Didik Pudji; Aji, Seto Purnomo; Handayani, Etty; Ratnasari, Tri; Jadmiko, Mochammad Wildan; Prayoga, Mohammad Candra; Khozin, Mohammad Nur; Kriswanto, Budi
PLANTA TROPIKA Vol 12, No 1 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i1.18063

Abstract

Porang (Amorphophallus muelleri B.) is a tuberous plant with the potential to be the main source of carbohydrates and is rich in benefits. Porang proliferation is limited by a 6-month dormancy period per year, and generative propagation is unlikely due to the seeds being apomictic triploid. The aimed of my research to analyze the application of BAP and NAA in culture media for direct propagation of porang shoots. The explant used in this research was young leaves. The research was arranged in a completely randomized design with a combination of BAP and NAA hormones added to the MS medium. There were three BAP treatments, namely 1.0 mg/L, 2.0 mg/L, and 3.0 mg/L, while NAA treatments consisted of 2 levels, namely 2.0 mg/L and 4.0 mg/L. The addition of 1.0 mg/L BAP combined with 4.0 mg/L NAA was the best treatment that produced seven shoots with an average shoot length of 2.14 cm and root length of 3.6 cm, with the earliest bud emergence (9.7 weeks after planting).