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DAYA HAMBAT EKSTRAK DAUN KENIKIR (Cosmos caudatus Kunth.) TERHADAP Staphylococcus aureus Leka Lutpiatina; Nur Rizqi Amaliah; Ratih Dewi Dwiyanti
Meditory : The Journal of Medical Laboratory Vol 5, No 2 (2017): meditory ,Volume 5, No 2, Desember 2017
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.129 KB) | DOI: 10.33992/m.v5i2.116

Abstract

Daun kenikir (Cosmos caudatus Kunth.) sudah lama dimanfaatkan masyarakat untuk dikonsumsi maupun pengobatan tradisional. Daun kenikir memiliki kandungan fenol, flavonoid, saponin dan tanin yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimal dan konsentrasi bunuh minimal ekstrak daun kenikir terhadap bakteri Staphylococcus aureus secara in vitro. Penelitian ini bersifat true eksperiment dengan rancangan postest only control group design. Variabel bebas dalam penelitian ini adalah konsentrasi ekstrak daun kenikir (Cosmos caudatus Kunth.).  Variabel terikat dalam penelitian ini adalah bakteri Staphylococcus aureus. Hasil penelitian Konsentrasi Hambat Minimal (KHM) menunjukkan kekeruhan pada konsentrasi 160 mg/ml, 320 mg/ml dan adanya kejernihan pada konsentrasi 340 mg/ml, 380 mg/ml dan 400 mg/ml. Hasil penelitian Konsentrasi Bunuh Minimal (KBM) diperoleh jumlah koloni pada konsentrasi 160 mg/ml sebanyak 60 CFU/ml, 320 mg/ml sebanyak 12 CFU/ml, 340 mg/ml sebanyak 3 CFU/ml, 380 mg/ml sebanyak 0 CFU/ml dan 400 mg/ml sebanyak 0 CFU/ml. Berdasarkan hasil penelitian dapat disimpulkan bahwa KHM ekstrak daun kenikir adalah 340 mg/ml dan KBM ekstrak daun kenikir adalah 380 mg/ml. Disarankan dilakukan penelitian lebih lanjut mengenai pengaruh ekstrak daun kenikir terhadap bakteri Staphylococcus aureus adalah dengan menggunakan pelarut lain.
Number of Breast Milk Bacteria in Women with Normal Labor and Caesarean Delivery During Room Temperature Storage Habibah, Siti Noor; Muhlisin, Ahmad; Lutpiatina, Leka; Insana, Aima
Tropical Health and Medical Research Vol. 6 No. 1 (2024): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v6i1.111

Abstract

The presence of microbes in each breast milk sample can be different, one of which is influenced by the method of delivery. The research aimed to analyze the effect of storage time for breast milk from mothers giving birth normally and giving birth by Caesarean on the number of bacteria at room temperature. The independent variable in this study was the storage time for breast milk from mothers giving birth normally and giving birth by Caesarean for 0, 4, 8, and 12 hours at room temperature, while the dependent variable in this study was the number of bacteria in the breast milk. The research samples were breast milk from mothers who gave birth normally and Caesarean section which was treated with storage at room temperature for 0, 4, 8, and 12 hours. The results of the research were that the average number of bacteria in breast milk from mothers giving birth normally, stored at 0, 4, 6, 8, and 12 hours respectively, was 1.33 x 104 CFU/ml; 2.18 x 104 CFU/ml; 5.04 x 106 CFU/ml; 1.68 x 107 CFU/ml. The average number of bacteria in breast milk from mothers giving birth by Caesarean storage 0, 4, 6, 8, and 12 hours respectively 1.83 x 104 CFU/ml; 1.73 x 105 CFU/ml; 1.76 x 107 CFU/ml; 6.67 x 107 CFU/ml. There is a significant difference in the number of bacteria between breast milk from mothers who gave birth normally and breast milk from mothers who gave birth by Caesarean section. Storage time has a positive effect on the number of bacteria in breast milk from mothers giving birth normally and giving birth by Caesarean. It is recommended to store breast milk at room temperature for up to 4 hours for breast milk from women giving birth normally and for breast milk from mothers giving birth by Caesarean, storage for less than 4 hours by paying attention to the cleanliness of the breast milk storage area.
Salmonella sp Contamination on Chicken Eggs and Duck Eggs at the Antasari Traditional Market, Banjarmasin, Indonesia Sa'adah, Sa'adah; Dwiyanti, Ratih Dewi; Lutpiatina, Leka
Tropical Health and Medical Research Vol. 6 No. 1 (2024): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v6i1.114

Abstract

Chicken eggs and duck eggs can be a source of transmission of Salmonellosis. Salmonella bacteria can penetrate the eggshell, or the egg's contents can become directly contaminated with Salmonella in the reproductive organs before the shell covers the egg. This research aims to analyze Salmonella sp in chicken and duck eggs at the Antasari Traditional Market in Banjarmasin, Indonesia. Samples were taken by purposive sampling with the criteria for cracked/broken eggs and intact eggs: 3 chicken eggs and three duck eggs from 6 traders so that the total sample was 24. For the identification of bacteria, microscopic tests and biochemical tests were used on the identification media. The results showed Salmonella sp in duck eggs and no Salmonella in chicken eggs. In all samples of chicken and duck eggs, 12.50% of Salmonella sp, 37.50% of Klebsiella sp, 12.50% of Proteus sp, and 37.50% of Pseudomonas sp were found. Suggestions for consumers are to pay more attention to the hygiene and sanitation of traders before buying eggs, choose clean eggs, store eggs in the refrigerator, and cook eggs until they are cooked.
Differences in Thawing Methods in Broiler Chicken Meat on Total Plate Count (TPC) of Bacteria Putri, Noorlaily; Lutpiatina, Leka; Nurlailah, Nurlailah; Insana, Aima
Tropical Health and Medical Research Vol. 6 No. 2 (2024): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v6i2.120

Abstract

Total Plate Count (TPC) determines the number of microorganisms, both bacteria and fungi. Thawing is a step in thawing food that was previously stored frozen. This study aims to analyze the differences in the Total Plate Count of bacteria with variations in thawing methods in broiler chicken meat. Thawing methods commonly used include thawing at room temperature, soaking in water, and leaving it in the refrigerator (chiller). The type of research is experimental design with a posttest-only control design. Repetition was carried out five times for each control group KN (negative control) and test groups R0 (fresh chicken), R1 (frozen chicken), R2 (chicken thawed at room temperature), R3 (chicken soaked in water) and R4 (chicken thawed in the chiller) then the number of bacteria that grew was calculated. The average number of R0 colony results ranged from 4.1 x 103 CFU/g, R1 8.6 x 102 CFU/g, R2 1.6 x 104 CFU/g, R3 8.9 x 103 CFU/g and R4 1.2 x 103 CFU/g. The Kruskall-Wallis test results showed an Asymp sig value of 0.000 (<0.05); it can be concluded that there are differences in TPC in broiler chicken meat with variations in thawing methods that are thawed at room temperature, soaked in water and thawing in the chiller. It is recommended to thaw frozen chicken meat using the method of leaving it in the refrigerator because it has the lowest number of germs and can inhibit the growth of germs.
Pelatihan Penurunan Tingkat Kekeruhan Air Sungai Di Desa Sungai Rangas Tengah Khair, Abdul; Noraida, Noraida; Lutpiatina, Leka
JPEMAS: Jurnal Pengabdian Kepada Masyarakat Vol. 1 No. 2 (2023): JPEMAS : Jurnal Pengabdian Kepada Masyarakat
Publisher : Yayasan Pendidikan Tanggui Baimbaian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71456/adc.v1i2.352

Abstract

Sebagian besar masyarakat di Desa Sungai Rangas Tengah memperoleh air untuk keperluan sehari-hari dari air sungai yang melintas di desa tersebut. Bila belum digunakan maka air disimpan di dalam drum atau tempat penyimpanan air lainnya. Diketahui tingkat kekeruhan air (56 NTU) melebihi persyaratan yang ditetapkan (< 25 NTU) meskipun pH masih sesuai (6,8). Ada masyarakat mengolah air sungai yang keruh menggunakan tawas, namun dosis tawas yang digunakan dan cara pengolahan yang dilakukan tidak sesuai. Hal tersebut mengakibatkan rasa air menjadi asam, sepat, bahkan terkadang air tidak berubah menjadi jernih. Kegiatan pengabdian kepada masyarakat di desa ini ditujukan untuk melatih masyarakat membuat sediaan tawas dan menurunkan tingkat kekeruhan air sungai untuk keperluannya sehari-hari. Lama waktu seluruh kegiatan adalah 6 bulan dengan melibatkan Sanitarian Puskesmas Martapura Barat. Jumlah sasaran sebanyak 50 kepala keluarga. Kegiatan utama berupa pemberian materi dan pelatihan tentang cara pengolahan air sungai menggunakan tawas. Hasil posttest dari penyuluhan dan pelatihan menunjukkan bahwa seluruh sasaran (100%) dapat menjawab pertanyaan dengan benar. Selanjutnya monitoring dan evaluasi dilakukan untuk melihat secara langsung cara penerapan pengolahan air sungai menggunakan tawas di rumah masyarakat. Diharapkan kegiatan membantu penyediaan air bersih bagi masyarakat di desa Sungai Rangas Tengah.
Antithrombotic Activity and Hemolysis Pattern of Fibrinolytic Protease-Producing Bacterial Isolates from the Coast of Tanjung Dewa, South Kalimantan Lutpiatina, Leka; Dwiyanti, Ratih Dewi; Insana, Aima; Rizal, Muhammad; Mufid, Muhammad
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.689

Abstract

Cardiovascular disease is a leading cause of death worldwide, with thrombosis playing a key role in the pathological process. Conventional antithrombotic therapy often causes side effects, necessitating alternatives based on biological resources. Marine microorganisms are known to produce fibrinolytic protease enzymes that can degrade fibrin and prevent blood clot formation. This study aimed to evaluate the differences in antithrombotic activity and hemolysis patterns of fibrinolytic protease-producing bacterial isolates obtained from the coast of Tanjung Dewa Beach, South Kalimantan. A total of 15 isolates were obtained from seawater, beach sand, mollusks, crabs, and barnacles, and then subjected to colony morphology identification, Gram staining, and spore observation. Proteolytic activity testing using Skim Milk Agar media showed that all isolates had proteolytic activity with varying indices, with PSR1 showing the highest index (3.4). Furthermore, fibrinolytic testing using the fibrin plate assay method showed that 10 isolates were capable of degrading fibrin, with AL8 and SP2 showing the highest fibrinolytic indices (3.12 and 3.11, respectively). Antithrombotic testing using the clot lysis method revealed that AL7 and SP1 exhibited the highest lysis percentages (82.05% and 88.88%, respectively). Anticoagulant activity, as determined by the Lee-White method, showed that SP2 significantly prolonged the coagulation time (142 seconds, 49.65%). Hemolysis pattern testing revealed variations in activity, with AL8 and PSR1 classified as gamma-hemolytic and therefore potentially safer. Statistical analysis showed no significant differences between isolates in terms of antithrombotic activity, anticoagulant activity, or hemolysis pattern (p > 0.05). These findings suggest that coastal bacterial isolates from Tanjung Dewa Beach, particularly AL8 and SP2, have the potential to serve as safe, natural antithrombotic agents, supporting the development of biomedical therapies for cardiovascular disease.
The Value of the TB IgG/IgM Rapid Test and Erythrocyte Sedimentation Rate in Relation to Xpert MTB/RIF Tests in the Diagnosis of Pulmonary Tuberculosis Sari, Endah Permata; Lutpiatina, Leka; Insana, Aima
Tropical Health and Medical Research Vol. 8 No. 1 (2026): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tuberculosis (TB) remains a global health problem, requiring healthcare workers to have accurate and effective diagnostic methods. The Xpert MTB/RIF-based Molecular Rapid Test is recommended as the standard for TB diagnosis, but in clinical practice, healthcare workers still frequently use the TB IgG/IgM Rapid Test and the Erythrocyte Sedimentation Rate (ESR) as complementary tests. This study aimed to determine the relationship between Xpert MTB/RIF test results and the TB IgG/IgM Rapid Test and ESR in pulmonary tuberculosis patients at Pertamina Hospital, Balikpapan. This study used a retrospective, observational analytical study design utilizing secondary data from laboratory tests from January–December 2024. Researchers analyzed data from 250 patients who met the inclusion criteria. They analyzed the relationship between variables using the Spearman correlation test. The results showed that 16.4% of patients had MTB detected on the Xpert MTB/RIF test. Statistical analysis showed a significant positive correlation between Xpert MTB/RIF results and the TB IgG/IgM Rapid Test, with a moderate correlation strength (r = 0.562; p < 0.001). Furthermore, the analysis also showed a significant correlation between Xpert MTB/RIF results and ESR (r = 0.183; p = 0.004), but with a very weak correlation strength. The majority of patients with MTB detected by Xpert MTB/RIF had positive TB IgG/IgM Rapid Test results and abnormal ESR values. This study shows that the TB IgG/IgM Rapid Test and ESR are associated with Xpert MTB/RIF results. However, both tests have limited specificity and can therefore only be used as supporting tests and cannot replace molecular testing in the diagnosis of pulmonary tuberculosis.
The Role of Biological and Environmental Factors in Infant Pneumonia: Findings from the 2023 Indonesian Health Survey Lutpiatina, Leka; Dwiyanti, Ratih Dewi; Insana, Aima
Tropical Health and Medical Research Vol. 8 No. 1 (2026): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v8i1.150

Abstract

Pneumonia continues to be a leading cause of morbidity and mortality in infants, particularly in developing countries, making risk factor analysis crucial to support evidence-based prevention efforts. This study analyzed factors associated with pneumonia in 28,610 infants aged one year and under using data from the 2023 Indonesian Health Survey to provide a national overview of the distribution of cases and their determinants. This study used a cross-sectional design and analyzed demographic variables, health characteristics, and environmental factors using descriptive analysis, chi-square tests, and odds ratios. The study results showed a pneumonia prevalence of 0.9%, with an increased risk in low birth weight infants (OR 2.425; 95% CI: 1.688–3.483) and infants with a history of diarrhea (OR 2.051; 95% CI: 1.211–3.475), while breastfeeding reduced the risk of pneumonia (OR 0.590; 95% CI: 0.447–0.778). The data also showed greater susceptibility among male infants and those living in urban areas. These findings confirm that pneumonia in infants is influenced by a combination of biological, environmental, and behavioral factors, so public health interventions need to strengthen monitoring of at-risk infants, increase the coverage and quality of breastfeeding, prevent diarrhea, and control exposure to air pollution, especially in urban areas. These findings provide an empirical basis for developing more targeted and effective infant health policies.
Monocyte, Lymphocyte, and Monocyte–Lymphocyte Ratio Responses to Treatment Phases in Pulmonary Tuberculosis Patients Restu, Nurul Hidayathul Mahpudinah; Lutpiatina, Leka; Muhlisin, Ahmad; Dwiyanti, Ratih Dewi
Tropical Health and Medical Research Vol. 8 No. 1 (2026): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v8i1.151

Abstract

Pulmonary tuberculosis is a chronic infectious disease that affects the balance of innate and adaptive immune responses, reflected in changes in hematological parameters, particularly monocyte and lymphocyte counts, and the monocyte-lymphocyte ratio (MLR). This study aimed to analyze differences in lymphocyte and monocyte counts and MLR values ??in pulmonary tuberculosis patients based on treatment phase. This cross-sectional analytical survey study involved 60 pulmonary tuberculosis patients divided into 0-month treatment groups, the intensive phase, and the continuation phase in Loa Kulu District from January to April 2025. Peripheral blood smears were stained with Giemsa to determine the percentage of lymphocytes and monocytes, and then the MLR value was calculated. Statistical analysis using one-way ANOVA showed significant differences in lymphocyte and monocyte counts between treatment groups (p<0.05). The mean lymphocyte count increased with treatment duration, while the mean monocyte count decreased, resulting in a decrease in the MLR value from 0.64 in the pre-treatment group to 0.29 in the intensive phase and 0.22 in the continuation phase. The decrease in MLR reflects an improved immune response balance and decreased systemic inflammation during tuberculosis therapy. The results of this study indicate that MLR demonstrated variation across treatment phases, suggesting its potential relevance in the clinical assessment of TB patients.
Co-Authors Abdul Khair Abdul Khair Ahmad Muhlisin Ahmad Muhlisin Annisa Nur Yulianti Annisa Rahmi Anny Thuraidah Dinna Rakhmina Elfira Maulidah Erpan Roebiakto Fadia, Fadia Farah Indah Swari Gina Alia Mawaddah Habibah, Siti Noor hana nailah Helmiah, Tini Elyn Insana, Aima jasmadi Joko Kartiko Mufid, Muhammad Muhammad Ma'ruf MUHAMMAD RIZAL Nastiti Megawati Nisa Nur Agistni Eriana Noraida Noraida Noraida Noraida, Noraida Nur Rizqi Amaliah Nurhilaliah Nurhilaliah Nurlailah Nurlailah, Nurlailah Putri, Noorlaily Raimunah Raimunah Ratih Dewi Dwiyanti Ratih Dewi Dwiyanti, Ratih Dewi Restu, Nurul Hidayathul Mahpudinah Rifqoh Rifqoh Sa'adah, Sa'adah Sari, Endah Permata Syaidatul Aslamiyah wahdah norsiah Wahidah Wahidah Widiyawati Widiyawati Yayuk Kustiningsih