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Journal : Konservasi Hayati

Isolasi Fungi Endofitik Pada Organ Kulit Batang Tanaman Gambir (Uncaria guianensis (Hunter) Roxb) Habisukan, Ummi Hiras; Nisa, Khoirun; Rosa Lia; Oktariyani Putri; Bella, Eken Vegita; Tini Karisma
Konservasi Hayati Vol 19 No 2 (2023): OKTOBER
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v19i2.28332

Abstract

Gambir (Uncaria guianensis (Hunter) Roxb) is a shrub from the Rubiaceae family which has hard, twisting stems, short-stemmed leaves, light green in color, small white flowers with round cobs. Gambir is already known to have many medicinal benefits. Lots of compounds contained in gambier which are useful as antioxidants. In Indonesia it is used for betel nut besides the betel plant. Another benefit of the gambier plant is as a mixture of medicines for burns, headaches, diarrhea, dysentery, mouthwash, canker sores, and as a skin tanner to prevent decay which makes the skin look softer, more colorful and youthful. This study aims to isolate the types of endophytic fungi found on the bark of the gambier plant and to identify the types of endophytic fungi based on their macroscopic and microscopic characteristics. The research method used in this study was a sample of Gambir (U. guianensis) taken from Hamlet V, Tambang Rambang Village, Rambang Kuang Subdistrict, Ogan Ilir Regency, South Sumatra. The endophytic fungal isolation method used was gambier plant stem bark samples using PDA media. Bark taken from the main stem and branches, exfoliated less than a full circle. The results of this study succeeded in obtaining 5 isolates of endophytic fungi from the bark of gambir (U. guianensis) belonging to 4 different genera, including Trichoderma, Blastomyces, Fusarium, and Alternaria.
Identifikasi Fungi Endofit yang Diisolasi dari Tanaman Jambu Monyet (Anacardium occidentale L.) pada Wilayah Kabupaten Banyuasin Habisukan, Ummi Hiras; Musyalina, Aisyah; Oktiansyah, Rian; Laksono, Pandu Jati
Konservasi Hayati Vol 19 No 1 (2023): APRIL
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v19i1.26570

Abstract

Cashew fruit is a plant that is widely grown in eastern Indonesia, such as in NTB, Bali, NTT, South Sulawesi, and Southeast Sulawesi. The study aimed to identify the endophytic fungi found in cashew trees. The method used in this research was taking samples, namely the roots, root bark, stems, bark and leaves of the cashew plant then making instant PDA media mixed with distilled water and antibiotics with direct planting techniques on PDA in Petri dishes. Furthermore, the purification of endophytic fungi was carried out followed by macroscopic and microscopic observations by observing the types of spores, spore forms, and hyphae. The results of macroscopic and microscopic observations on samples of isolates coded KB.AO1 and D.AO1 were identified as belonging to the genus Fusarium, isolates coded KB.AO2 belonged to the genus Trichoderma, isolates coded A.AO1 belonged to the genus Colletotrichum, and isolate A.AO2 belonged to the genus Acremonium, isolate KB.AO1 belongs to the genus Cladosporium, code KB.AO2 belongs to Aspergillus, isolate code BAO1 belongs to Pythium, isolate code BAO2 belongs to Mucor, and isolate code DAO2 belongs to Nigrospora.