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All Journal Medical Laboratory Technology Journal Jaringan Laboratorium Medis Tropical Health and Medical Research Journal Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Karya Generasi Sehat
Ratih Dewi Dwiyanti, Ratih Dewi
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Journal : Medical Laboratory Technology Journal

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Formulation Test of Dracontomelon dao (Blanco) Merr. & Rofe Leaf Extract Powder as an Anti-Diarrheal Escherichia coli Method In Vivo Dwiyanti, Ratih Dewi; Nurlailah, Nurlailah; Thuraidah, Anny
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.556

Abstract

Dracontomelon dao (Blanco) Merr. & Rofe is one of the medicinal plants used in the community. This study aims to analyze the effectiveness of  Dracontomelon dao (Blanco) Merr. & Rofe extract powder as an antidiarrheal against Escherichia coli in vivo. Dracontomelon dao (Blanco) Merr. & Rofe leaf comes from Hulu Sungai Utara, South Kalimantan, Indonesia, and is macerated with 96% ethanol—Dracontomelon dao extract powder (Blanco) Merr. & Rofe is made with three parts thick extract, four parts Lactose, and four parts Starch. Experimental rat were conditioned to develop diarrhea and were treated with extract powder with varying doses per kg of body weight, namely 250 mg, 500 mg, and 750 mg. After treatment on day 6, rat recovered from diarrhea in the 750 mg dose treatment group as much as 78%, while the positive control total recovery on day 6 reached 100%. After treatment on day 6, the number of Escherichia coli colonies in rat feces decreased, namely the lowest in the 750mg dose treatment group, 276 colonies/gr. This research concludes that Dracontomelo dao (Blanco) Merr. & Rofe leaf extract powder is effective as an anti-diarrhea against Escherichia coli in experimental rat. Further research is needed on human test subjects to obtain anti-diarrhea drugs with safer and more effective formulations.
Antithrombotic Activity and Hemolysis Pattern of Fibrinolytic Protease-Producing Bacterial Isolates from the Coast of Tanjung Dewa, South Kalimantan Lutpiatina, Leka; Dwiyanti, Ratih Dewi; Insana, Aima; Rizal, Muhammad; Mufid, Muhammad
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.689

Abstract

Cardiovascular disease is a leading cause of death worldwide, with thrombosis playing a key role in the pathological process. Conventional antithrombotic therapy often causes side effects, necessitating alternatives based on biological resources. Marine microorganisms are known to produce fibrinolytic protease enzymes that can degrade fibrin and prevent blood clot formation. This study aimed to evaluate the differences in antithrombotic activity and hemolysis patterns of fibrinolytic protease-producing bacterial isolates obtained from the coast of Tanjung Dewa Beach, South Kalimantan. A total of 15 isolates were obtained from seawater, beach sand, mollusks, crabs, and barnacles, and then subjected to colony morphology identification, Gram staining, and spore observation. Proteolytic activity testing using Skim Milk Agar media showed that all isolates had proteolytic activity with varying indices, with PSR1 showing the highest index (3.4). Furthermore, fibrinolytic testing using the fibrin plate assay method showed that 10 isolates were capable of degrading fibrin, with AL8 and SP2 showing the highest fibrinolytic indices (3.12 and 3.11, respectively). Antithrombotic testing using the clot lysis method revealed that AL7 and SP1 exhibited the highest lysis percentages (82.05% and 88.88%, respectively). Anticoagulant activity, as determined by the Lee-White method, showed that SP2 significantly prolonged the coagulation time (142 seconds, 49.65%). Hemolysis pattern testing revealed variations in activity, with AL8 and PSR1 classified as gamma-hemolytic and therefore potentially safer. Statistical analysis showed no significant differences between isolates in terms of antithrombotic activity, anticoagulant activity, or hemolysis pattern (p > 0.05). These findings suggest that coastal bacterial isolates from Tanjung Dewa Beach, particularly AL8 and SP2, have the potential to serve as safe, natural antithrombotic agents, supporting the development of biomedical therapies for cardiovascular disease.
Co-Authors Ahmad Muhlisin Aisya Nahdiya Anggraini, Agnina Listya Anny Thuraidah, Anny Azmi, Dini Aulia fahani, Ayu Hafidh, Muhammad haitami haitami, haitami Insana, Aima Ipniliati, Eva leka lutpiatina, leka Mufid, Muhammad MUHAMMAD RIZAL Neni Oktiyani, Neni Nurlailah Nurlailah, Nurlailah Nurul Huda Pinto Laksono, Fransiskus Xaverius Agung Pratama, Haris Nurdin Rifqoh Rifqoh, Rifqoh Rizkiyah, Risa Sa'adah, Sa'adah Sutiany, Ayu Sya’adah, Nurul Laili Ulfah, Putriana Yuli Susanti