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Saskia L. Nasroen, Saskia L.

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Author-ID : 631530

Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Other

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Optimalization of DNA isolation from oral epithelial mucous cell with smear method Nasroen, Saskia L.; Maskoen, Ani Melani; Nurwiadh, Agus
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Deoxyribonucleic acid (DNA) is a genetic material which is found in all living organisms. On the human cell or eukaryotes cell, the DNA is found in the nucleus cell and the mitochondria. The DNA arrangement on each cell in human body is the same, that is why, for the analysis meaning, DNA can be isolated from any cell in the body. The source of DNA to be analyzed usually coming from the blood sample by an injection method, such a way resulting in pain and bringing about constraint. Therefore, a study was carried out to look for an alternative of DNA isolation. The aim of this experimental study was to get an optimal DNA isolation method by using oral mucous smear method with a purpose to get a quick and easy DNA isolation. The investigation materials were in the form of samples which were taken from the oral epithelial mucous cells out of three different subjects. The epithelial cells were obtained by the oral mucous smear method which in a variation of two, four and six times of smear applications, respectively. The DNA was then isolated using buffer extraction method. The concentrations of DNA were measured by using ultraviolet spectrophotometer at 260 nm wavelength. The results of DNA isolation were analyzed by Polymerase Chain Reaction (PCR) technique. The optimal DNA isolation could be analyzed by PCR technique. The experimental results show that from three different subjects of study, DNA can be isolated optimally by oral mucous smear method with six times of smear applications.

Optimalization of DNA isolation from oral epithelial mucous cell with smear method Nasroen, Saskia L.; Maskoen, Ani Melani; Nurwiadh, Agus
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November 2009
Publisher : Universitas Padjadjaran

Deoxyribonucleic acid (DNA) is a genetic material which is found in all living organisms. On the human cell or eukaryotes cell, the DNA is found in the nucleus cell and the mitochondria. The DNA arrangement on each cell in human body is the same, that is why, for the analysis meaning, DNA can be isolated from any cell in the body. The source of DNA to be analyzed usually coming from the blood sample by an injection method, such a way resulting in pain and bringing about constraint. Therefore, a study was carried out to look for an alternative of DNA isolation. The aim of this experimental study was to get an optimal DNA isolation method by using oral mucous smear method with a purpose to get a quick and easy DNA isolation. The investigation materials were in the form of samples which were taken from the oral epithelial mucous cells out of three different subjects. The epithelial cells were obtained by the oral mucous smear method which in a variation of two, four and six times of smear applications, respectively. The DNA was then isolated using buffer extraction method. The concentrations of DNA were measured by using ultraviolet spectrophotometer at 260 nm wavelength. The results of DNA isolation were analyzed by Polymerase Chain Reaction (PCR) technique. The optimal DNA isolation could be analyzed by PCR technique. The experimental results show that from three different subjects of study, DNA can be isolated optimally by oral mucous smear method with six times of smear applications.

Pemeriksaan, Penyuluhan Kesehatan Gigi, Sikat Gigi Bersama, dan Pemberian Flourida Topikal pada Siswa SD Binaan Puskesmas Saguling Bandung Barat Hartman, Henri; Yuslianti, Euis Reni; Arifianti, Isti; Dewi, Zwista Yulia; Nasroen, Saskia L.; Rahaju, Asih; Trisusanti, Ratna; Djohan, Frita FS.; el Fitriyah, Rhabiah; Suntana, Mutiara S.; Maskoen, Ani Melanie; Isnaeni, Rheni S.; Khaerunnisa, Rahmadaniah; Isryad, Widya; Adinugraha, Irham M.; Fathonah, Dea Isny
Jurnal Abdimas Kartika Wijayakusuma Vol 6 No 3 (2025): Jurnal Abdimas Kartika Wijayakusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v6i3.631

Kegiatan pengabdian masyarakat ini bertujuan meningkatkan pengetahuan dan praktik kesehatan gigi serta mulut pada siswa dan guru binaan Puskesmas Saguling, Kabupaten Bandung Barat. Pelaksanaan kegiatan dilakukan di SD Cikande dan SD Cibodas, dengan target sasaran sebanyak 435 siswa dan 40 guru. Tim pelaksana terdiri dari 15 dosen Fakultas Kedokteran Gigi, mahasiswa sarjana, koas, serta 15 dokter gigi Persatuan Dokter Gigi Indonesia (PDGI) Cabang Bandung Barat. Metode yang digunakan dalam kegiatan mencakup pemeriksaan kesehatan gigi dan mulut, pemeriksaan berat badan, tinggi badan, dan indeks massa tubuh, serta aplikasi fluorida topikal. Penyuluhan kesehatan diberikan melalui media edukasi berupa alat peraga boneka, flipchart, PowerPoint, dan video. Kegiatan ini diakhiri dengan kegiatan sikat gigi bersama untuk memperkuat pemahaman siswa tentang teknik menyikat gigi yang benar. Hasil dari kegiatan ini menunjukkan peningkatan pengetahuan peserta tentang pentingnya menjaga kesehatan gigi dan mulut, serta keterlibatan dalam kegiatan aplikatif seperti sikat gigi bersama dan penggunaan fluorida topikal.

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