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All Journal Jurnal Ilmu dan Teknologi Kesehatan Medicra (Journal of Medical Laboratory Science/Technology) Humanism : Jurnal Pengabdian Masyarakat Camellia
Ellies Tunjung Sari Maulidiyanti, Ellies Tunjung Sari
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Religion Humanities Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Journal : Medicra (Journal of Medical Laboratory Science/Technology)

 1 
The Effect of Storage Time for Pooled Sera on Freezers on the Quality of Clinical Chemical Examination: Pengaruh Lama Penyimpanan Pooled Sera pada Freezer Terhadap Mutu Pemeriksaan Kimia Klinik Maulidiyanti, Ellies Tunjung Sari; Purwaningsih, Nur Vita; Widiyastuti, Rahma; Samsudin, Rinza Rahmawati; Arimurti, Anindita Riesti Retno
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1613

Abstract

A common problem in clinical laboratories is maintaining the stability of serum analytes during sample storage. Samples are usually stored in the door (4–8°C) of the refrigerator for a short period of time or in the freezer (-20°C) for a longer period of time (pre-analytical stage) and subsequently, during post-analysis sample handling (storage time and temperature). This study aims to analyze the stability in this case is the storage time of pooled sera in the freezer at temperatures up to -18º C and in the refrigerator at temperatures up to 4°C for 3 months with the inspection parameters, namely cholesterol and triglycerides. This type of research is experimental, with a research design that is time series control group design. The sample in this study was a control serum in the form of pooled sera stored in the freezer and refrigerator which was checked for cholesterol and triglyceride levels every 2 weeks for 3 months or for 12 weeks. The results showed that the average calculation of cholesterol levels in pooled sera stored in the freezer was 142.07 mg/dL and the average cholesterol level examination stored in the refrigerator was 161.89 mg/dL then the average the examination of triglyceride levels in pooled sera stored in the freezer is 112.89 mg/dL and the average examination of triglyceride levels stored in the refrigerator is 140.46 mg/dL. Based on the analysis of the research results, it can be concluded that, there is an effect of storage time in the freezer on the stability of cholesterol levels and triglyceride levels.
Potential of Seed Powder (Tamarindus indica L.) on Cholesterol Levels: Potensi Seduhan Serbuk Biji Asam Jawa (Tamarindus indica) Terhadap Kadar Kolesterol Purwaningsih, Nur Vita; Widyastuti, Rahma; Maulidiyanti, Ellies Tunjung Sari; Saputro, Tri Ade; Rohmayani, Vella; Ainutajriani, Ainutajriani
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1713

Abstract

ABSTRACT Coronary heart disease (CHD) is the leading cause of death after stroke in all age groups at 12.9%. The main reason is the amount of cholesterol in the blood. Tamarind (Tamarindus indica) is a traditional herb that has the potential to lower cholesterol, because it contains flavonoids, saponins, foliphenols, pectin, organic acids, vitamin B and vitamin C that can lower cholesterol. The purpose of this study was to determine the effect of tamarind seed powder (Tamarindus indica) on cholesterol levels. This type of research is experimental with pre-test and post-test with control group research design. The population and sample of this study were a group of mice totaling 32 mice. The location of this research was conducted at the Animal Husbandry Center (PUSVETMA). Data collection procedures were carried out by examining the cholesterol levels of mice before and after treatment. The data obtained were followed up with normality test and independent t test. The results of the study using the unpaired t test gave the result of p=0.000 (p<0.05). The conclusion of this study is that there is an effect of seed powder (Tamarindus indica) on cholesterol levels. Keyword : Seeds Tamarindus indica L., cholesterol levels, Mice (Mus musculus)
Cellular Immune Responses and Hepatitis B Surface Antigen (HBsAg) in Alcohol-Consuming Fishermen : Respon Imun Seluler dan HBsAg pada Nelayan Pengonsumsi Alkohol Purwaningsih, Nur Vita; Saputro, Tri Ade; Azizah, Fitrotin; Maulidiyanti, Ellies Tunjung Sari; Budiman, Waras; Anggraini, Lukita
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 8 No. 2 (2025): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v8i2.1803

Abstract

Hepatitis B infection remains a global health problem with long-term complications such as cirrhosis and hepatocellular carcinoma. Alcohol consumption is known to impair immune function and exacerbate hepatitis B virus (HBV) infection. Fishermen represent a high-risk occupational group due to alcohol consumption habits and limited healthcare access. This study aimed to analyze the relationship between alcohol consumption levels and hepatitis B infection status through HBsAg testing and lymphocyte count among fishermen. This was a descriptive observational study with a cross-sectional approach involving 40 fishermen in Gresik Regency. Data were collected through alcohol consumption questionnaires and laboratory tests, including HBsAg examination using a Rapid Test and lymphocyte count using a hematology analyzer. Data were analyzed using Chi-square and ANOVA tests with a significance level of p < 0.05. A total of 30% of respondents were HBsAg reactive, with the highest proportion found in the heavy alcohol consumption group (50%). The mean lymphocyte count decreased with increasing alcohol intake: 2.580 ± 420 cells/µL in the light group, 2.120 ± 390 cells/µL in the moderate group, and 1.780 ± 360 cells/µL in the heavy group (p = 0.012). There was a significant relationship between alcohol consumption level, HBsAg status, and lymphocyte count among fishermen. High alcohol consumption may reduce cellular immunity and increase the risk of persistent hepatitis B infection. HBsAg testing and lymphocyte count can serve as early indicators for health screening in high-risk occupational groups such as fishermen. 
Co-Authors Ainutajriani Ainutajriani, Ainutajriani Andika Aliviameita Anggraini, Lukita Anindita Riesti Retno Arimurti Budiman, Waras Diah Ariana DITA ARTANTI Fitrotin Azizah, Fitrotin Hidayat, Mariza Nur Vita Purwaningsih, Nur Vita Rahma Widyastuti Rinza Rahmawati Samsudin Rohmayani, Vella Saputro, Tri Ade Widiyastuti, Rahma Yusuf, Muhammad Bilal