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All Journal Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education JFM (Jurnal Farmasi Malahayati) Jurnal Pengabdian Farmasi Malahayati (JPFM) Jurnal Ilmiah Wahana Pendidikan Publikasi Hasil Pengabdian Kepada Masyarakat.
Mastuti Widianingsih, Mastuti
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Health Professions Medicine & Pharmacology Public Health Social Sciences Other

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Phytochemical Study and Determination of Minimum Inhibitory Concentration (MIC) of Rambutan Leaf (Nephelium lappaceum L.) Extract Against Salmonella typhi and Bacillus cereus: Studi Fitokimia Dan Penentuan Kadar Hambat Minimum (KHM) Ekstrak Etanolik Daun Rambutan (Nephelium lappaceum L.) Terhadap Salmonella thypi dan Bacillus cereus Nabila, Dewi; Widianingsih, Mastuti
JRST (Jurnal Riset Sains dan Teknologi) Volume 10 No. 1, March 2026: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v10i1.28803

Abstract

Infections caused by Salmonella typhi and Bacillus cereus represent a significant public health concern, exacerbated by increasing antibiotic resistance. Rambutan (Nephelium lappaceum L.) leaf extract contains bioactive compounds with potential antibacterial activity, however its efficacy against these pathogens remains underexplored. The objective of this research to evaluate the antibacterial activity of ethanol extract of rambutan leaves against S. typhi and B. cereus by determining the Minimum Inhibitory Concentration (MIC). The research included plant identification, ethanol maceration extraction, phytochemical screening, and antibacterial assessment using the broth dilution method. The extract yielded 13.97%, and phytochemical analysis revealed the presence of flavonoids, alkaloids, tannins, saponins, terpenoids, and phenolic compounds. The MIC of the extract was 25% for both bacteria, with S. typhi exhibiting higher sensitivity. The antibacterial mechanism is presumed to involve interactions of secondary metabolites with bacterial cell membranes and nucleic acid components. These findings highlight the potential of rambutan leaf extract as a promising antimicrobial agent for further development.   ABSTRAK (Bahasa Indonesia) Infeksi akibat Salmonella typhi dan Bacillus cereus menjadi masalah kesehatan yang serius dengan meningkatnya resistensi antibiotik. Ekstrak daun rambutan mengandung senyawa bioaktif potensial antibakteri, namun efektivitasnya terhadap kedua bakteri tersebut belum banyak diteliti. Penelitian ini bertujuan mengevaluasi aktivitas antibakteri ekstrak etanol daun rambutan terhadap S. typhi dan B. cereus melalui penentuan Konsentrasi Hambat Minimum (KHM). Penelitian meliputi tahap identifikasi tanaman, ekstraksi dengan metode maserasi menggunakan etanol, screening fitokimia, dan uji aktivitas antibakteri melalui metode dilusi cair untuk menentukan Konsentrasi Hambat Minimum (KHM) ekstrak terhadap S. typhi dan B. cereus. Ekstrak daun rambutan menghasilkan rendemen 13,97%. Analisis fitokimia menunjukkan keberadaan flavonoid, alkaloid, tanin, saponin, terpenoid, dan senyawa fenolik. Hasil uji antibakteri menunjukkan KHM ekstrak terhadap S. typhi dan B. cereus masing-masing 25%, dengan S. typhi lebih sensitif. Mekanisme antibakteri diduga terkait interaksi metabolit sekunder dengan membran sel dan komponen nukleat bakteri. Temuan ini menegaskan ekstrak daun rambutan berpeluang dikembangkan lebih lanjut sebagai bahan antimikroba.
Uji Aktivitas Antibakteri Sediaan Obat Kumur Dari Enzim Bromelin Ekstrak Bonggol Nanas (Ananas comosus (L.) Merr.) TERHADAP Staphylococcus aureus Retnaningsih, Agustina; Antika, Sindi; Widianingsih, Mastuti
Jurnal Ilmiah Wahana Pendidikan Vol 12 No 5.A (2026): Jurnal Ilmiah Wahana Pendidikan
Publisher : Peneliti.net

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Abstract

Dental caries is one of the most prevalent oral diseases, can be associated with infection by Staphylococcus aureus. Pineapple stem is often considered waste, actually it contains bromelain enzyme with potential antimicrobial properties. This research aimed to utilize pineapple stem as a source of bromelain for mouthwash formulation, and to evaluate its antibacterial effect against Staphylococcus aureus. Bromelain was extracted using ammonium sulfate precipitation and incorporated into mouthwash formulation at concentrations of 2%, 4%, and 6%. The formulations demonstrated acceptable physical characteristics, including pale yellow color, pineapple mint aroma, refreshing mint taste, and viscosity and pH values within standard range, indicating good physical stability. Antibacterial testing using the paper disc diffusion method showed that none of the formulations produced inhibition zones, similar to the negative control. In contrast, the positive control (Betadine mouthwash) inhibited a 2.4 mm inhibition zone. These result indicate that bromelain extract from pineapple stem did not exhibit significant antibacterial activity against Staphylococcus aureus the tested conditions.
Co-Authors Adyana, Ketut Sudi Antika, Sindi Fauziyah, Sekar Gunawan, Rayhan Fatih Ilham Firman Ashari Laisya, Nashwa Putri Marbun, Rustian Afencius Marcelia, Selvi Muhammad Yusuf Nabila, Dewi Ningrum, Uut Astia Praseptiawan, Mugi Putri, Rahma Febrilia Retnaningsih, Agustina Revangga, Dwi Arthur Rinjani, Ripi Vania Adha Setiawan, Muhammad Arif Setyorini, Dian Catur Sianturi, Elsa Elisa Yohana Sinaga, Nydia Renli Siregar, Abu Bakar Siddiq Tutik Tutik Untoro, Meida Cahyo