Hary Purnamaningsih
Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan Universitas Gadjah Mada. Jl. Fauna No.2, Karangmalang, Depok, Sleman. 55281 Yogyakarta

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Journal : Jurnal Veteriner

Isolasi dan Identifikasi Microsporum canis dari Anjing Penderita Dermatofitosis di Yogyakarta (ISOLATION AND IDENTIFICATION OF Microsporum Canis FROM DERMATOPHYTOSIS DOGS IN YOGYAKARTA) Soedarmanto Indarjulianto; Yanuartono .; Hary Purnamaningsih; Puspa Wikansari; Gerson Yohanes Imanuel Sakan
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.223 KB)

Abstract

Dermatophytosis in dogs can be caused by one species of dermatophytes group called Microsporumcanis. This study aims to isolation and identification of M. canis in dogs suspected dermatophytosis inYogyakarta. Skin scrapings from 50 dogs that clinically showed lesions such as combination of alopecia,erythema, papules, pustules, scaly and crusty used in this study. Samples of skin scraping were culturedin the Sabouraud’s dextrose agar media for fungi identification macroscopically and microscopically. Theresults showed that 17 of 50 samples (34%) grown on SDA medium from 2 to 18 days after cultivation. Thecolony grew with flat topography and slightly reflexed, the surface of the colony looks like a thick fur, whitein the middle and surrounded by brownish yellow color and the edges were colorless. The opposite surfaceof the colony looks flat and slightly reflexed and orange to brown and the edges were colorless. Observationmicroscopically, the fungi showed a large macroconidia with a thick cell wall and contains 6-12 cells andoval microconidia with a small size and found in few along the hyphae. Based on the research it can beconcluded that 17 of 50 (34%) samples of dogs with dermatophytosis are Microsporum canis.
Infeksi Microsporum canis pada Kucing Penderita Dermatitis (MICROSPORUM CANIS INFECTION IN DERMATITIS CATS) Soedarmanto Indarjulianto; Yanuartono Yanuartono; Sitarina Widyarini; Slamet Raharjo; Hary Purnamaningsih; Alfarisa Nururrozi; Nurman Haribowo; Hizriah Alief Jainudin
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.373 KB) | DOI: 10.19087/jveteriner.2017.18.2.207

Abstract

Dermatitis in cats can be caused by Microsporum canis and is zoonotic. This study aims to perform clinical examination, laboratory examination, isolation, and identification of mold M. canis in cats with dermatitis. Skin scrapped from 30 cats that clinically showed lesions of dermatitis i.e. combination of alopecia, erythema, papules, pustules, scaly, and crusty were used in this study. Examination of clinical lesions and screening tests using the Wood’s lamps were performed prior to sampling. Skin scrapes samples were cultured onto Sabouraud’s dextrose agar medium, incubated at 28 ° C and colonies were identified macroscopically then stained using Lactophenol Cotton Blue for microscopic examination. Seventen of the 30 samples (56.7%) were identified as M. canis macroscopically and microscopically. The skin lesions observed in the 17 M. canis infected cats were erythema, alopecia, scaly, and crusty distributed to the ear, body, neck, back and tail of cats, respectively ABSTRAK Dermatitis pada kucing dapat disebabkan oleh Microsporum canis dan bersifat zoonotik. Penelitian ini bertujuan melakukan pemeriksaan klinis, pemeriksaan laboratoris, isolasi, dan identifikasi kapang M. canis pada kucing penderita dermatitis. Kerokan kulit dari 30 ekor kucing yang secara klinis menunjukkan lesi dermatitis berupa kombinasi dari alopesia, eritema, papula, pustula, bersisik, dan berkerak digunakan dalam penelitian ini. Pemeriksaan lesi klinis dan uji screening dengan lampu Wood’s dilakukan sebelum pengambilan sampel. Sampel kerokan kulit dikultur pada media Sabouraud’s dextrose agar, diinkubasi pada suhu 28°C dan diidentifikasi secara makroskopis. Koloni yang tumbuh diperiksa secara mikroskopis menggunakan pewarnaan Lactophenol Cotton Blue. Hasil penelitian menunjukkan 17 dari 30 sampel (56,7%) teridentifikasi M. canis secara makroskopis dan mikroskopis. Lesi kulit yang ditemukan pada 17 kucing positif terinfeksi M. canis menunjukkan adanya eritema, alopesia, bersisik, dan berkerak dengan lokasi penyebaran pada telinga, badan, leher, punggung atau ekor.