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All Journal Jurnal Analis Kesehatan Klinikal Sains (Jurnal Analis Kesehatan) JHECDs: Journal of Health Epidemiology and Communicable Diseases Borneo Journal Of Pharmascientech KESANS : International Journal of Health and Science Indonesian Community Journal
Putri Kartika Sari
Universitas Borneo Lestari, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health Social Sciences Other

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PENGARUH VARIASI LAMA PENYIMPANAN LARUTAN TURK MODIFIKASI AIR PERASAN JERUK NIPIS (Citrus Aurantifolia) TERHADAP HITUNG JUMLAH LEUKOSIT hanipa sapria; Norlaila Puteri Maulida; Ratna Amanah Sari; Putri Kartika Sari
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 1 (2024): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i1.4086

Abstract

The availability of Turk reagents for manual leukocyte count examinations, commonly performed by simple clinics or remote health centers, is often limited. This limitation can arise due to depletion or expiration of the reagents. Consequently, many researchers have sought to address this issue by developing modified Turk solutions, including those utilizing freshly squeezed lime juice, although the quality of these alternative reagents remains untested. This study aims to investigate the effects of storing modified Turk solutions with lime juice (Citrus aurantifolia) at room temperature for 1 day, 7 days, and 14 days on leukocyte count. This experimental research employed a Post Test Only Control Group Design, with variations in storage duration 1, 7, 14 days replicated 12 times and analyzed statistically. The mean leukocyte counts were 6.083 cells/mm3 at 1 day, 7.612 cells/mm3 at 7 days, and 6.304 cells/mm3 at 14 days, while the control exhibited a count of 7.675 cells/mm3. The results of the ANOVA and LSD tests indicated significant differences in the leukocyte counts between day 1 and day 14 with the control Turk, whereas day 7 did not show such differences, however, the interpretation of the leukocyte count results for the lime-modified Turk solution on days 1, 7, and 14 still indicated compliance with the reference values, i.e., 4,000-10,000 cells/mm3.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT DAUN TIGARON (Crataeva nurvala Buch. Ham) TERHADAP Staphylococcus aureus DENGAN METODE SUMURAN Putri Kartika Sari; Nanda Amelia Rahmi; Fitriyanti Fitriyanti
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5121

Abstract

Staphylococcus aureus infection is characterized by tissue damage accompanied by purulent abscesses. Antibiotics are a group of drugs using to treat and prevent bacterial infections. However, long-term use causes antibiotic resistance. Efforts made to increase antibiotic resistance include using natural ingredients as alternative treatments. Tigaron is an empirical medicinal plant whose leaves are used to treat abscesses, wounds and skin diseases. This research aims to determine the types of secondary metabolite compounds and antibacterial activity against Staphylococcus aureus from ethyl acetate extract of tigaron leaves (Crataeva nurvala Buch. Ham). This research was experimental with a post test only control group design with 7 concentration variations. Extraction made by maceration using ethyl acetate, identification of secondary metabolite using phytochemical screening, antibacterial activity tested using well diffusion method. The results showed that concentration ​​of 0.31 mg/mL, 1.55 mg/mL and 7.75 mg/mL were in medium category. Concentrations of 38.75 mg/mL and 193.75 mg/mL were in strong category. Chloramphenicol as a positive control had strongest inhibition zone diameter. The conclusion are ethyl acetate extract of tigaron leaves contain alkaloid, flavonoid and steroid which inhibiting the growth of Staphylococcus aureus, so it has the potential to be a natural antibiotic using to treat infectious diseases
Co-Authors Ainun Rizqina Aldiana Astuti Dian Nurmansyah Eka Fitri Susiani fitriyanti fitriyanti Fitriyanti, Fitriyanti hairunnisa nisa hanipa sapria Muhammad Arsyad Muhammad Arsyad Muhammad Arsyad Muhammad Fahmi Muhammad Nazarudin Muhammad Raja Perdana Nafila Nafila, Nafila Namira Al Medina Nanda Amelia Rahmi Norlaila Puteri Maulida Nurbidayah Nurbidayah Nurbidayah Puspawati Puspawati Rabiatul Munawarah Ratna Amanah Sari Rifqoh Rifqoh Riwanda Oktaviani Rizka Ayu Wahyuni Rufi'i Syahrizal Ramadhani