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Hyperglicemia as Predisposition Factor of Oral Candidiasis on patient with Diabetes Mellitus Dian Nurmansyah; Elma Stasya; Dewi Ramadhani; Normaidah Normaidah; Aldiana Astuti
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.703

Abstract

Diabetes mellitus is a disease characterized by hyperglycemia or an increase in blood glucose levels. The state of hyperglycemia is one of the supporting factors for oral candidiasis infection. The purpose of this study was to determine the correlation of hyperglycemia with the incidence of Candida albicans infection in patients with Diabetes mellitus. this study was conducted on 30 respondents. Blood glucose levels and Candida albicans culture were measured using the germ tube test method. Data analysis to see the correlation using the Chi-Square test obtained Sig. (0.015 0.00.050) and it can be stated there is a correlation between variables. From the results of data analysis, it can be concluded that there is a correlation between hyperglycemia and Candida albicans infection which causes oral candidiasis in DM patients.
An overview of Microfilariae on patients who have received treatment in Batuah Village, Kotawaringin Timur District 2015 Dian Nurmansyah; Muhammad Fahmi; Rifqoh Rifqoh; Puspawati Puspawati; Putri Kartika Sari; Muhammad Arsyad; Aldiana Astuti
JHECDs: Journal of Health Epidemiology and Communicable Diseases Vol 4 No 2 (2018): JHECDs Vol. 4, No. 2, Desember 2018
Publisher : Balai Litbangkes Tanah Bumbu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.552 KB) | DOI: 10.22435/jhecds.v4i2.378

Abstract

Filariasis is a chronic infectious disease, caused by filarial worms in the lymph and lymph nodes cause lymphangitis and elephantiasis. Batuah Village is the area in Sampit with the largest percentage of microfilariae compared to the surrounding area. From 196 the number of Batuah villagers examined by the filariasis elimination officer of Kotawaringin Timur District Health Office in 2013 obtained 5 positive cases of filariasis with 2.55% microfilaria number which is dominated by filaria worm Brugia malayi. From the local health center has also conducted a mass drug treatment program with DEC medication, combined with albendazole for 12 days on 14-16 July 2014. The aim of this study was to determine the success rate of treatment conducted by the health officer against filariasis patients in the Batuah village. The method used in this microfilariae examination is a microscopic method. Capillary blood was taken on 4 filarial patients who tested positive on the previous examination who had been given DEC + Albendazole treatment, the result from 4 samples that examined 1 positive sample of 4 patients. The implementation of medication that has been done by public health office was successful because there was a decrease of microfilaria rate which was previously 2.55% to 0,51% (<1%).
IMUNOPATOLOGI DAN PERSPEKTIF LABORATORIUM KLINIK SARS - COV - 2 : A REVIEW Dian Nurmansyah; Aldiana Astuti
Klinikal Sains : Jurnal Analis Kesehatan Vol 8 No 2 (2020): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v8i2.1406

Abstract

Severe Acute Respiratory syndrome corona virus 2 ( SARS-Cov-2) is a virus from Nidoviridales family Coronaviridae that causes acute respiratory syndrome in humans. The virus discovered in wuhan, China in mid December 2019 and determined as a global pendemic by WHO The management of the patient becomes crucial in suppressing the mortality rate of patients with positive COVID-19. The actual viral infection is self-limited disease with supportive treatment. Good treatment support requires information and clarity of viral pathogenesis, laboratory results, patient clinical symptoms and in-depth analysis of virus characteristics and the body's immune response to infection
Uji Antikoagulan Alami Ekstrak Kayu Manis (Cinnamomum Burmanni) Menggunakan Metode Lee-White dan Analisis Apusan Darah Aldiana Astuti; Theresia Mooy
Jurnal Ilmu Kesehatan dan Gizi Vol. 2 No. 4 (2024): Oktober: Jurnal Imu Kesehatan dan Gizi
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jig.v2i4.3213

Abstract

Coumarin, a key bioactive compound in cinnamon, is known for its anticoagulant, antibiotic, and anti-carcinogenic properties. This study investigates the potential of cinnamon (Cinnamomum burmanni) extract as a natural anticoagulant using both the Lee-White method, for visual observation of clotting time, and blood smear analysis for microscopic evaluation. The research aims to assess the effectiveness of the extract in preventing blood coagulation compared to untreated blood samples. Results demonstrated that blood without any anticoagulant clotted within an average time of 5 minutes and 33 seconds. In contrast, blood treated with cinnamon extract did not exhibit coagulation, indicating a significant anticoagulant effect. Microscopic examination further supported these findings, as the blood cells in the treated samples appeared separated, with no visible aggregation or linkage between the cells. These findings suggest that cinnamon extract has strong potential as a natural anticoagulant, offering a safer and more accessible alternative to synthetic anticoagulants. Further research could explore its applications in medical treatments, particularly in conditions requiring blood thinning or clot prevention. This study highlights the potential of cinnamon as a valuable bioactive agent in the field of biomedical science, especially for anticoagulation therapies.
Penyuluhan dan Pemeriksaan Feses Pada Masyarakat Desa Bone Kecamatan Nekamese Sebagai Upaya Pencegahan Penyakit Tropis Aldiana Astuti; Wilhelmus Olin; Marni Tangkelangi; Adrianus Ola Wuan
Natural: Jurnal Pelaksanaan Pengabdian Bergerak bersama Masyarakat. Vol. 2 No. 2 (2024): May : Natural: Jurnal Pelaksanaan Pengabdian Bergerak bersama Masyarakat
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/natural.v2i2.426

Abstract

Hookworm infection remains a significant health concern, particularly in rural areas like Bone Village, Nekamese District. This community service project aimed to conduct health education and stool examinations to prevent and control tropical diseases. Stool examinations were performed on 60 samples from the Bone Village community, revealing that 2 samples tested positive for hookworm eggs, indicating the presence of hookworm infection, although the prevalence was relatively low. Following this, an interactive health education session was conducted on March 24, 2024, at the village church, involving the local community. The educational materials covered the prevention and treatment of hookworm infection, the importance of personal and environmental hygiene, and how to detect early symptoms of tropical diseases. This activity successfully raised community awareness about the importance of preventing tropical diseases and was published in the online media Kupang, reaching a broader audience. The results of the community service project demonstrated that a participatory and educational approach is effective in reducing the risk of hookworm infection and improving overall community health
OPTIMIZING MOLECULAR TECHNIQUES FOR ACCURATE EXAMINATION OF LEPTOSPIRA SPECIES: A COMPREHENSIVE PRIMER FOR RESEARCHERS Aldiana Astuti; Farida Dwi Handayani
Journal of Vocational Health Studies Vol. 8 No. 2 (2024): November 2024 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V8.I2.2024.108-115

Abstract

Background: Leptospirosis is a potentially life-threatening disease caused by bacteria of the genus Leptospira. The accurate identification and characterization of Leptospira species are critical for disease surveillance, outbreak investigation, and treatment strategies. Molecular techniques, such as Polymerase Chain Reaction (PCR) and Deoxyribonucleic acid (DNA) sequencing, have revolutionized the field of microbiology, providing rapid and accurate identification of Leptospira strains. However, optimizing these molecular techniques for accurate examination of Leptospira species can be challenging due to the genetic diversity and complexity of these bacteria. Purpose: This research aims to identify the most suitable primers for the precise identification of pathogenic Leptospira strains. Method: This research used the PCR method, using LipL32, rrs2, seqY, LipL41, IcdA, and Adk primers. A total of 17 isolates of pathogenic Leptospira bacteria were cultured from Institute of Vector Control and Reservoir Disease (IVRCD) in Salatiga, Indonesia. Result: The results of the research showed that the LipL41 and IcdA primers were found to be effective in distinguishing pathogenic strains, while the seqY, LipL32, Adk, and rrs2 primers required further refinement. The suitable Melting Temperature (TM) or annealing temperature is 58°C with 35 cycles of amplification. DNA concentration and purity had an A260/A280 ratio ranging between 1.8 and 2.8. Conclusion: LipL41 (500 bp) and IcdA (700 bp) are suitable primers for identifying pathogenic Leptospira.
Gerakan Sd Negeri Bone Hidup Sehat Bebas Diare Di Desa Bone Kecamatan Nekamese Tahun 2024 Novian A Yudhaswara; Agnes Rantesalu; Ni Ketut Yulianasari; Aldiana Astuti; Marni Tangkelangi
Sevaka : Hasil Kegiatan Layanan Masyarakat Vol. 3 No. 1 (2025): Februari : Sevaka : Hasil Kegiatan Layanan Masyarakat
Publisher : STIKES Columbia Asia Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62027/sevaka.v3i1.288

Abstract

Cleanliness of the school environment, health of the student environment in the teaching and learning process, based on the analysis of the situation above, especially the location of the school partner and conversations with the principal and teaching staff. This activity began with an interview, it was found that students did not have maximum knowledge about health, especially diarrhea, as evidenced by the pre-test interview 70% answered correctly.The expected output of this program is the awareness of school administrators, students about clean living patterns that must be applied in the school environment in order to create student welfare. So that in the long term, a clean living pattern has been embedded in all school children and from there it is hoped that it can create a school environment, especially school children who are prosperous and free from disease. In the socialization, the Team also demonstrated the implementation of a clean living pattern in the school environment such as washing hands with soap and cleaning up trash properly by SDN Bone students. At the end of the activity, a post-test was carried out with the same questions randomly, 90% of the answers were correct, the school hopes that this educational movement can increase the knowledge of teachers and students as an effort to improve student health and prevent diarrhea.
Pemeriksaan Golongan Darah pada Anak dan Pemeriksaan Gula Darah pada Kelompok Usia Lanjut di Gereja Katolik Santu Petrus Pariti Kabupaten Kupang Tahun 2024 Theresa Avila Meman; Aldiana Astuti; Muhammad Aldizar; Yoseph Maran; Mervi Berelaku
Bumi : Jurnal Hasil Kegiatan Sosialisasi Pengabdian kepada Masyarakat Vol. 2 No. 4 (2024): Bumi: Jurnal Hasil Kegiatan Sosialisasi Pengabdian kepada Masyarakat
Publisher : Asosiasi Riset Teknik Elektro dan Informatika Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/bumi.v2i4.443

Abstract

This community service activity was conducted at the Catholic Church of Santu Petrus Pariti, Kupang Regency, involving 124 participants for blood type examinations and 69 participants for blood pressure and glucose level tests. The event began with a retreat by the Medical Laboratory Technology students aimed at raising community awareness about health. A positive response was observed through the enthusiasm of the community, who actively asked questions, indicating that they gained new knowledge related to health examinations. In blood type examinations conducted at TK Santa Maria Pariti and SEKAMI, it was found that among 18 female and 22 male children, 5 participants had blood type A, 15 had blood type B, 17 had blood type O, and 1 had blood type AB. Additionally, glucose level tests revealed that 4 participants had normal glucose levels, while 65 had abnormal levels. Blood pressure tests among the elderly showed 16 with abnormal readings and 53 with normal readings. The examination results provided an initial overview of the health status of the local community, highlighting variations in blood types, glucose levels, and blood pressure conditions. This activity is expected to enhance awareness of the importance of regular health check-ups among the community.
Gambaran C-Reactive Protein Pada Pasien Tuberkulosis Paru di Puskesmas Naibonat Kabupaten Kupang Ni Ketut Yuliana Sari; Adrianus Ola Wuan; Aldiana Astuti; Novian Agni Yudhaswara; Agnes Rantesalu; Ikhsan Rasidin
Quantum Wellness : Jurnal Ilmu Kesehatan Vol. 1 No. 4 (2024): Desember: Quantum Wellness : Jurnal Ilmu Kesehatan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/quwell.v2i1.1280

Abstract

Tuberculosis is a chronic infectious disease induced by the bacterium Mycobacterium tuberculosis and can attack human organs, especially the lungs. The entry of this antigen causes the release of various pro-inflammatory cytokines which cause liver cells to synthesize acute phase proteins such as C-Reactive Protein (CRP). C-Reactive Protein is a type of alpha globulin protein that appears in the blood in response to inflammation in the body. The aim of this study was to determine the description of CRP in pulmonary tuberculosis patients at the Naibonat Community Health Center. The method used is descriptive with a cross sectional study design method. This research was carried out at the Naibonat Health Center Laboratory, Kupang Regency from February to April 2024. The examination method used was the qualitative latex agglutination method. The results of CRP examination on pulmonary TB patients from a total of 23 respondents obtained reactive results for 8 samples (35%) and non-reactive results for 15 samples (65%). Based on the results of reactive CRP, the highest number was found in males, 6 people (26%), while the highest number was found in the age range 12-25 years, 4 patients (Teenagers) (17%), with the highest length of treatment at 0 -2 months (intensive stage) as many as 6 patients (35%), with the highest patient status being new TB as many as 8 patients (35%), with the highest treatment status being non-resistant as many as 6 patients (26%).
Utilizing Maize Cob Media as a Replacement for Nutrient Agar Media in the Growth of Escherichia coli and Staphylococcus aureus Bacteria Aldiana Astuti; Ni Made Susilawati; Agnes Peni
International Journal of Educational Research Vol. 1 No. 2 (2024): June: International Journal of Educational Research
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/ijer.v1i2.23

Abstract

Bacteria are microorganisms that require adequate media and nutrients to grow and develop. The media commonly used to grow bacteria is Nutrient agar media, but the price of Nutrient agar media is quite expensive. Corn cobs are lignocellulosic wastes that contain nutrients that can meet the nutritional requirements of bacterial growth media. This study aims to investigate the viability of corn cobs as a cost-effective alternative to Nutrient agar media. Specifically, we explore the variance in bacterial colony growth when utilizing corn cobs as a growth medium for Escherichia coli and Staphylococcus aureus bacteria. Employing a true experimental design with a posttest-only control structure, we analyze the treatment's effects using the One Way ANOVA test and Independent Sample T-test. These statistical methods enable us to discern differences between groups utilizing corncob media with and without added sugar.Our findings reveal noteworthy insights into bacterial colony growth. For Escherichia coli, the average number of colonies on corncob media with sugar was 222 CFU/ml, on corncob media without sugar, it was 164 CFU/ml, and on Nutrient agar media, it reached 361 CFU/ml. In the case of Staphylococcus aureus, colonies on corncob media with sugar averaged 871 CFU/ml, on corncob media without sugar, they averaged 657 CFU/ml, and on Nutrient agar media, they reached 942 CFU/ml.Statistical analysis using the One Way ANOVA test for both Escherichia coli and Staphylococcus aureus demonstrated p-values <0.05, signifying significant differences in their growth on corncob media with sugar, corncob media without sugar, and Nutrient agar media. Furthermore, the Independent Sample T-test results for both bacterial strains yielded p-values <0.05, indicating dissimilar growth patterns on corncob media with sugar compared to corncob media without sugar.In conclusion, our study underscores significant disparities in the growth of Escherichia coli and Staphylococcus aureus bacteria among Nutrient agar media, corncob media with sugar, and corncob media without sugar. Notably, we observed a marked increase in bacterial colony counts on corncob media with sugar and corncob media without sugar.