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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Penelitian Pendidikan IPA (JPPIPA)
Abdul Ashari
Tadulako University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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A Study of the NGF Gene Intron in the Philippine Scrubfowl (Megapodius cumingii) from Kabetan Island, Central Sulawesi Wayan Windyawati; I Made Budiarsa; Zulaikhah Dwi Jayanti; Manap Trianto; Abdul Ashari; I Nengah Kundera
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1353-1361

Abstract

The Philippine scrubfowl (Megapodius cumingii) is a bird species that utilizes environmental heat to incubate its eggs and has a wide geographic distribution across various habitats, including coastal forests, coastal areas, and small islands. This study aimed to describe and analyze the genetic diversity and phylogenetic relationships of Megapodius cumingii based on the Nerve Growth Factor (NGF) gene. Genomic DNA was isolated from blood samples of M. cumingii using the gSYNC™ DNA Extraction Kit (Geneaid) protocol and subsequently amplified using the Polymerase Chain Reaction (PCR) method with the forward primer AllNGF5' and reverse primer AllNGF3'. Sequence data were analyzed using BLAST to determine their similarity to reference sequences available in GenBank. Genetic diversity was assessed using DnaSP v6.12.03, while phylogenetic reconstruction was performed using MEGA 11 based on the Neighbor-Joining (NJ) method with the Kimura 2-Parameter model. The results showed that the nucleotide composition consisted of Adenine (A) 29.3%, Thymine (T) 19.8%, Cytosine (C) 26.9%, and Guanine (G) 24.1%, with a G+C content (51.0%) slightly higher than the A+T content (49.0%). Genetic diversity analysis revealed a haplotype diversity value of 0.533 ± 0.172 and a nucleotide diversity value of 0.00145 ± 0.00047. Phylogenetic reconstruction produced a consistent tree topology across two outgroup models, with all M. cumingii samples forming a single clade supported by high bootstrap values (99–100%). The phylogenetic relationships among M. cumingii samples from Kabetan Island, Tolitoli, were very close, as indicated by genetic distance values ranging from 0.000 to 0.003.
Co-Authors Abd. Hakim Laenggeng Akram Akram Gamar B. N. Shamdas I Made Budiarsa I Made Budiarsa I Nengah Kundera Jihan Winarti Manap Trianto Mia Pinasti Azizah Mohammad Jamhari Mursito S. Bialangi Musdalifah Nurdin Nur Amal Nurul Afiat Pebi Angreni Rafiqa Rafiqa Rafiqah Rizka Fardha Syech Zainal Vita Indri Febriani Wayan Windyawati Windarsih, Yulia Yosphita Sevianti Zulaikhah Dwi Jayanti