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All Journal CHEMICA Jurnal Teknik Kimia Jurnal Pengabdian Magister Pendidikan IPA Makara Journal of Science
Marno, Septhian
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Chemical Engineering, Chemistry & Bioengineering Education Energy

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Effect of Red and Blue Light on Lipid, Protein, Carbohydrate, and Pigment Contents of Navicula sp. Maulana, Sofyan; Nurul Hida, Aulia Setyo; Wulandari, Ismia; Mudrikah, Siti; Kurnianto, Dedy; Maghfiroh, Khusnul Qonita; Amelia, Ria; Erfianti, Tia; Putri, Renata Adaranyssa Egistha; Devi Anggraini, Irika; Marno, Septhian; Suyono, Eko Agus Agus
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

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Abstract

Light quality critically modulates the physiology of diatoms, influencing photosynthetic efficiency and metabolite synthesis. This study examines how monochromatic red (620–750 nm) or blue (450–495 nm) light affects the biochemical profiles and pigment production in Navicula sp. versus white light (control). Cultures were grown in sterile f/2 medium, and biomass was tracked via OD680. Biochemical analyses included lipids (Bligh and Dyer method), carbohydrates (phenol-sulfuric acid assay), proteins (Bradford assay), and pigments (spectrophotometric quantification). The growth dynamics were modeled using logistic and Gompertz equations. No significant differences in OD-based growth rates, carbohydrates, proteins, or lipids content were observed with red or blue light compared with the control. However, blue light elevated chlorophyll a levels 4-fold and maximized protein productivity 3-fold, whereas red light enhanced carotenoid content by 20.5-fold. The growth kinetics modeling that best matched the experimental data was the Gompertz modeling (R² > 0.649). These results demonstrate the wavelength-specific regulation of pigment and protein yields, suggesting that tailored light regimes can selectively optimize high-value metabolites in Navicula sp. This approach holds promise for biotechnology sectors prioritizing targeted compound production, such as nutraceuticals or biofuels, by leveraging spectral tuning to enhance metabolic efficiency and predicting particular growth phases using growth kinetics.
Introduksi Teknologi Budidaya Rumput Laut Sargassum sp. Untuk Produksi Bioethanol Cokrowati, Nunik; Asri, Yuliana; Lumbessy, Salnida Yuniarti; Affandi, Rangga Idris; Muahiddah, Nuri; Sukartono; Marzuki, Muhammad; Rahmadani, Thoy Batun Citra; Anggraini, Irika Devi; Marno, Septhian
Jurnal Pengabdian Magister Pendidikan IPA Vol 7 No 2 (2024): April-Juni
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v7i2.8185

Abstract

Abstract: Sargassum sp. seaweed is included in the category of brown macro algae that has not been cultivated and has not been optimally utilized. Sargassum sp. has economic value because it contains alginate and iodine which are utilized in the food, pharmaceutical, cosmetic, and textile industries. Sargassum sp. has a high carbohydrate content that can be converted into bioethanol and bioenergy. So that this potential can be developed to improve the community's economy and increase efforts to protect the environment from pollution from the use of fossil fuels. Sargassum sp. seaweed can be found in the waters of Gerupuk, Sengkol Village, Pujut District. Gerupuk is a hamlet that is included in the Mandalika Creative Economic Zone, among the community's livelihood activities is seaweed cultivation. Seaweed farmers in Gerupuk only cultivate K. alvarezii seaweed. They only know Sargassum sp. as a seaweed that grows wild and has no economic value. Sargassum sp. in Gerupuk is not utilized, so it needs to be educated on its benefits and utilization. This community service activity will be carried out in Gerupuk Hamlet, Sengkol Village, Pujut District, Central Lombok Regency. The output targets of this community service activity are appropriate technology for the cultivation of Sargassum sp. for bioethanol production, publications at national community service seminars and articles published in national community service journals indexed by sinta. The stages of implementing this activity are coordination of activities, counseling, demonstration of Sargassum sp. seaweed cultivation, mentoring, evaluation of activities, and collaboration with the Lombok Marine Aquaculture Center (BPBL). The expected results of this activity are that seaweed farmers in Gerupuk can cultivate Sargassum sp. to improve the household economy and the community economy. Keywords: Seaweed, Sargassum sp., cultivation, bioenergy, bioethanol.
Extraction of High Economic Potential of Lipids from Heterotrophic Cultivation of Indigenous Aurantiochytrium Microalgae Strain Suhendra, Suhendra; Anggraini, Lia; Nuryasari, Fazhar; Hutari, Andri; Anggraini, Irika Devi; Marno, Septhian
CHEMICA: Jurnal Teknik Kimia Vol. 10 No. 2 (2023): August 2023 [Available online since October 02, 2023]
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/chemica.v10i2.26021

Abstract

Aurantiochytrium microalgae has long been recognized as oleaginous microalgae since its capability to produce high content of lipids biomass. Produced lipids from cultivation process of Aurantiochytrium can contain high concentration of omega-3 docosahexanoic acid, hence valuable biomass from the Aurantiochytrium microalgae has the potential to be used as a source of raw materials for nutrition, cosmetics and medicines. Generally,  Aurantiochytrium microalgae can be found in mangrove ecosystems. Even Indonesia is ranked as the largest mangrove in the world, but the use of Aurantiochytrium sp microalgae is rarely explored in Indonesia. In order to optimize biomass utiisation produced the cultivation process, there shall be an optimum extraction process. Therefore, this study present the optimization of lipids from the Aurantiochytrium sp microalgae. The isolate used in this research comes from Bunaken mangrove forest, North Sulawesi, Indonesia. The cultivation process consists of three steps, namely standing culture (SC, 48 hours), pre-culture (PC, 48 hours) and standing culture (SC, 120 hours). The cultivation took pace in an erlemeyer flask using orbital shaker with an orbital speed of 220 rpm at room temperature and pressure. The average dried biomass was 9.4 g/L. In addition to cultivation process, recent paper presents also an extraction methodology using organic solvents, namely methanol, chloroform, acetone, ethyl acetate, and n-hexane. The lipids fcation can be extracted with a minimum of 7% lipid and a  maximum of 47% lipid. From this study, extraction using acetone-chloroform-ethyl acetate solvents resulted in the highest fraction of lipids from the extraction process of Aurantiochytrium biomass. Since the paper discussing the extraction process of native Indonesia strain of Aurantiochytrium microalgae has never been presented before, therefore this paper shall be valuable basis for further research in this field.
Co-Authors Amelia, Ria Anggraini, Irika Devi Asri, Yuliana Devi Anggraini, Irika Erfianti, Tia Hutari, Andri Kurnianto, Dedy Lia Anggraini Lumbessy, Salnida Yuniarti Maghfiroh, Khusnul Qonita Maulana, Sofyan Muhammad Marzuki Nunik Cokrowati Nuri Muahiddah Nurul Hida, Aulia Setyo Nuryasari, Fazhar Putri, Renata Adaranyssa Egistha Rahmadani, Thoy Batun Citra Rangga Idris Affandi siti mudrikah Suhendra Suhendra Sukartono Suyono, Eko Agus Agus Wulandari, Ismia