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All Journal Devotion: Journal of Research and Community Service Biofaal Journal Rumphius Pattimura Biological Journal
Moniharapon, Debby Dijola
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Biofaal Journal

 1 
UTILIZATION OF MALE BREADFRUIT (Artocarpus altilis) AS A BIOLARVACIDE FOR Anopheles sp. Moniharapon, Debby Dijola; Nindatu, Maria; Unitly, Adrien Jems Akiles; Sikafir, Beatrix Belina
Biofaal Journal Vol 4 No 1 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i1pp31-39

Abstract

Penelitian ini bertujuan untuk mengetahui efek biolarvasida ekstrak etanol bunga sukun jantan (Artocarpus altilis) terhadap mortalitas larva nyamuk Anopheles sp. vektor malaria. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 6 perlakuan dan 3 kali ulangan: A : Kontrol negatif (Aquades), B: Kontrol positif (Abate), C: Konsentrasi (Ekstrak etanol bunga sukun jantan) 1%, D: Konsentrasi (Ekstrak etanol bunga sukun jantan) 3%, E: Kosentrasi (Ekstrak etanol bunga sukun jantan) 6%, F: Konsentrasi (Ekstrak etanol bunga sukun jantan) 9%. Hasil yang diperoleh dianalisis dengan Analysis of Variance (ANOVA) kemudian dilanjutkan Uji Duncan pada taraf nyata α=0,05 menggunakan perangkat lunak SAS. Populasi dalam penelitian ini adalah larva Anopheles sp. yang didapat dari tempat perindukan larva nyamuk di dalam rumah (in door). Sampel dalam penelitian ini adalah 450 larva Anopheles sp. instar III pada masing-masing diletakan dalam 18 gelas plastik, yang masing-masing plastik berisi 25 ekor larva Anopheles sp. instar III. Sampel diadaptasikan selama seminggu, kemudian diberi perlakuan selama 12 jam. Hasil penelitian menunjukkan adanya efek biolarvasida Ekstrak etanol bunga sukun jantan (Artocarpus altilis) terhadap larva nyamuk Anopheles sp., konsentrasi ekstrak etanol bunga sukun jantan (Artocarpus altilis) yang efektif adalah 6%, yang mampu membunuh larva nyamuk Anopheles sp. dengan presentasi mortalitias 72% pada jam ke-12, dan 50% mortalitas (LC50) larva Anopheles sp. terjadi pada konsentrasi ekstrak etanol bunga sukun jantan konsentrasi 1.25%.
Efficacy Test of Ethanol Extract From Matoa (Pometia pinnata) Stem Bark on Aedes aegypti Larval Mortality As An Effort to Control Dengue Fever Moniharapon, Debby Dijola; Moniharapon, Mechiavel
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp1-9

Abstract

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus and transmitted primarily through the bite of the Aedes aegypti mosquito, the disease's primary vector. Controlling the population of this mosquito species is considered a strategic measure in preventing the spread of DHF. One potential biological control agent is the matoa plant (Pometia pinnata), a member of the Sapindaceae family widely distributed in tropical regions. This plant contains secondary metabolites such as flavonoids, tannins, and saponins, which exhibit natural insecticidal activity. This study aims to evaluate the efficacy of ethanol extract derived from the bark of P. pinnata against A. aegypti larvae. The extraction process was conducted using ethanol as a solvent, and the resulting extract was tested against mosquito larvae at concentrations of 25 ppm, 50 ppm, 70 ppm, and 90 ppm. Larval mortality data were analysed using Analysis of Variance (ANOVA) via SPSS software version 24.00. The results demonstrated that the ethanol extract of P. pinnata bark had a significant dose-dependent effect on larval mortality. These findings suggest the potential of the extract as a natural active ingredient in the formulation of plant-based larvicides, contributing to environmentally friendly and sustainable dengue vector control strategies.
JUMLAH SEL LEYDIG DAN SEL SERTOLI TIKUS GALUR SPARAGUE-DAWLEY TERPAPAR SOPI PASCA DITERAPI EKSTRAK ETANOL SIRIH CINA (Peperomia pellucida L.) Unitly, Adrien Jems Akiles; Killay, Amos; Moniharapon, Mechiavel; Eddy, La; Silahooy, Veince B; Huwae, Laury Marcia Ch; Moniharapon, Debby Dijola; Lakesubun, Bella Frida
Biofaal Journal Vol 5 No 1 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i1pp010-018

Abstract

Tujuan penelitian ini untuk mengetahui jumlah sel Leydig dan sel Sertoli tikus terpapar sopi pasca diterapi ekstrak etanol sirih cina (Peperomia pellucida. L). Rancangan acak lengkap (RAL) digunakan dalam penelitian ini, dengan membagi 15 ekor tikus ke dalam 5 kelompok perlakuan yang diulang sebanyak 3 kali, yaitu kelompok 5.4 adalah 3 ekor tikus yang diberi sopi 5.4 ml/ekor/hari selama 14 hari (kontrol negatif), kelompok Vit. C 6.3 adalah 3 ekor tikus yang diberi sopi 5.4 ml/ekor/hari kemudian diberi Vitamin C 6.3 mg/ekor/hari selama 14 hari (kontrol positif), kelompok 0.71, 1.43 dan 2.86 adalah 3 ekor tikus yang diberi sopi 5.4ml/ekor/hari selama 14 hari kemudian kemudian masing-masing kelompok diberi ekstrak etanol sirih cina 0.71g/ekor/hari selama 14 hari, 1.43g/ekor/hari selama 14 hari dan 2.86g/ekor/hari selama 14 hari. Penelitian ini dilakukan di Laboratorium Zoologi dan Mikroteknik Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Pattimura Ambon mencakup persiapan hewan model, pemberian minuman sopi, ekstraksi sirih cina, pembuatan preparat histologis dan pengamatan. Hasil yang diperoleh dianalisis dengan Analysis of Variance (ANOVA) dan dilanjutkan dengan uji duncan pada taraf nyata a = 0.05 menggunakan perangkat lunak SAS dan dilanjutkan dengan uji beda nyata terkecil untuk mengetahui perbedaan perlakuan yang diberikan. Hasil penelitian menunjukan bahwa pemberian sopi 5.4 ml dapat menyebabkan penurunan jumlah sel Leydig dan sel Sertoli dan setelah diberi ekstrak etanol sirih cina, jumlah sel Leydig dan sel Sertoli mengalami peningkatan, dimana dosis ekstrak etanol sirih cina yang baik untuk sel Leydig adalah 2.86g dan untuk sel Sertoli adalah dosis 1.43g.
The Effect of Clove Leaves (Syzygium aromaticum L) on Controlling the Maize Weevil (Sitophilus zeamis) Infestation in Stored Maize Seeds Moniharapon, Debby Dijola; Tetiwar, Karina Novalin
Biofaal Journal Vol 6 No 2 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i2pp149-158

Abstract

Storage is a crucial post-harvest handling process; however, during storage, agricultural products are susceptible to various types of deterioration. Post-harvest losses reach approximately 15–20% annually, with about 0.5–2% of those losses caused by the maize weevil (Sitophilus zeamais). Clove (Syzygium aromaticum L.) is an important spice plant, with its most commercially valuable part being the flower buds, which are predominantly used in the cigarette industry (around 80–90%). Clove leaves, which produce a characteristic aroma, contain an essential oil component called eugenol, known for its fungicidal, bactericidal, nematicidal, and insecticidal properties. This study aimed to determine the effectiveness and optimal weight of clove leaves (Syzygium aromaticum L.) in controlling Sitophilus zeamais. The research employed a Completely Randomized Design (CRD) consisting of five treatments and three replications. The treatments included K1 = 0 g (control, without clove leaves); K2 = 10 g clove leaves per 100 g maize; K3 = 15 g clove leaves per 100 g maize; K4 = 20 g clove leaves per 100 g maize; and K5 = 25 g clove leaves per 100 g maize. Observations were conducted once for each replicate, resulting in three data points per treatment. The collected data were analyzed using ANOVA. The results indicated that clove leaves can be used to control maize weevil (Sitophilus zeamais M.) infestations in stored maize. A total clove leaf weight of 70 g resulted in the highest mortality rate, with 9.08 individuals, yet this treatment was still not considered effective as it did not achieve more than 80% mortality of Sitophilus zeamais
Co-Authors Adrien Jems Akiles Unitly Amos Killay Delly Wakano, Delly La Eddy Lakesubun, Bella Frida LAURY CHARA HUWAE Martha Kaihena Moniharapon, Mechiavel Nindatu, Maria Sikafir, Beatrix Belina Silahooy, Veince B Tetiwar, Karina Novalin