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All Journal Hasanuddin Economics and Business Review Jurnal Riset Sains dan Kimia Terapan Makara Journal of Science Jurnal Kesehatan Masyarakat
Fitriyanti, Anisa
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Chemistry Economics, Econometrics & Finance Education Public Health

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Journal : Jurnal Kesehatan Masyarakat

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hpmA Gene as a Detection Method of Proteus mirabilis Bacteria using real-time Polymerase Chain Reaction Nurjayadi, Muktiningsih; Putri, Gladys Indira; Fitriyanti, Anisa; Declan, Jefferson Lynford; Novitasari; Abomoelak, Bassam
Jurnal Kesehatan Masyarakat Vol. 21 No. 2 (2025)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v21i2.16214

Abstract

Proteus mirabilis is pathogenic bacteria that can cause gastrointestinal infections, bacteremia, and Urinary Tract Infections (UTI). Therefore, it is necessary to have a fast, sensitive, specific, and accurate detection method to identify Proteus mirabilis. This study aims to determine the confirmation, specificity, and sensitivity test of the hpmA gene primer to detect Proteus mirabilis quick and precise using the real-time Polymerase Chain Reaction method. Gradient Polymerase Chain Reaction results showed the hpmA primer has an amplicons length of 195 bp and the optimum annealing temperature at 60°C. The primer pair produced Ct value of 10.40±0.18 and showed one peak in the melting curve with Tm value of 81.84°C±0.02 by real-time PCR. Furthermore, the hpmA primer was also able to distinguish target and non-target bacteria based on the difference in Ct and Tm value formed. Based on these results, the concentration of bacterial DNA that can be detected by primers reached 3.2 pg/μL, equivalent to the concentration of target bacteria that can be detected by primers is 10.24×102 CFU. In the next step, hmpA primer will be developed to detect Proteus mirabilis in artificial contaminated samples using real-time PCR.
Co-Authors Aboemolak, Bassam Abomoelak, Bassam Agus Setiawan Angelina, Helzi Azizah, Puan Aqila Berkahingrum, Ayu Chen, Shyi-Tien Dalia Sukmawati Elenshasy, Hesham Ali Enshasy, Hesham Ali El Grace, Grace Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartika, Irma Ratna Kartini Kartini Krisdawati, Ismaya Kurniadewi, Fera Muhammad Husni Muhammad Husni Muktiningsih Nurjayadi Musie, Royna Rahma NOVITASARI Putri, Gladys Indira Putri, Gusti Angieta Rahmawati, Atikah Nur Saamia, Vira Sihombing, Ananda Indah Putri Siti Fatimah Wiranatha, I Made