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All Journal Jurnal Farmasi Sains dan Komunitas Jurnal Riset Kesehatan Poltekkes Depkes Bandung Jurnal Ilmiah Kesehatan JMLS: Journal of Medical Laboratory and Science Current Biomedicine Jurnal Kesehatan Siliwangi
Sufa, Hafizah Ilmi
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Current Biomedicine

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Confirmation of Mycobacterium tuberculosis culture results with Ziehl-Neelsen staining and MPT64 antigen test Indrasari, Witri; Kurniati, Iis; Dermawan, Asep; Sufa, Hafizah Ilmi
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.11

Abstract

Background Culture of Mycobacterium tuberculosis (MTB) using egg-based solid media like Lowenstein Jensen (LJ) is the gold standard for tuberculosis diagnosis but requires extended incubation time. Rapid diagnostic tests, such as Ziehl-Neelsen (ZN) staining and the MPT64 antigen rapid test, are essential for early detection. Objective This study aimed to evaluate the effectiveness of ZN staining and the MPT64 rapid test in detecting MTB and Mycobacterium other than tuberculosis (MOTT) during different culture times. Methods Using a cross-sectional design, 110 culture-positive samples were analyzed from Hasan Sadikin Hospital Bandung over two months. Specimens were cultured on LJ media for eight weeks, with weekly observation of colony growth. ZN staining and MPT64 tests were performed on growing colonies. Results Less than four weeks culture, 61 samples (55.5%) were culture-negative, 45 (40.9%) were positive for MOTT, and the remainder were contaminated. In more than four weeks culture, 48 samples (43.6%) were positive for MTB, 45 samples (40.9%) were positive for MOTT, and 13 (11.8%) were culture-negative, and the remaining were contaminated. ZN-positive and MPT64-negative results indicated MOTT in less than four weeks culture, while ZN-positive and MPT64-positive results indicated MTB in more than four weeks culture. Conclusion While ZN staining was positive for both MTB and MOTT colonies, the MPT64 rapid antigen test was specific for MTB, supporting its use in confirming MTB detection alongside culture methods.
Effect of solvent variation on results of antibiotic susceptibility test using the disk diffusion method against Staphylococcus aureus Habibah, Firda Nurul; Sufa, Hafizah Ilmi; Kurniati, Iis; Rismiarti, Zuri
Current Biomedicine Vol. 3 No. 2 (2025): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.2.60

Abstract

Background Mueller-Hinton agar (MHA) is widely used for disk diffusion tests to assess antibiotic susceptibility in non-fastidious bacteria. The type of water used to prepare the MHA may have affected the test outcomes. Objective This study evaluated the effect of different water types as solvents on the antibiotic susceptibility test results of tetracycline and gentamicin against Staphylococcus aureus. Methods MHA was prepared using four types of water: distilled (control), bottled, reverse osmosis (RO), and municipal tap water (PDAM). Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method, and data were analyzed using One-Way ANOVA and Tukey’s Post Hoc test. Results The mean inhibition zones for tetracycline were 23.8 ± 0.99 mm (distilled), 23.4 ± 0.37 mm (bottled), 23.0 ± 0.43 mm (RO), and 17.8 ± 1.41 mm (tap), categorized as sensitive except for tap water (intermediate). For gentamicin, the zones were 11.7 ± 0.37 mm (distilled), 12.7 ± 0.77 mm (bottled), 9.8 ± 0.18 mm (RO), and 17.6 ± 1.19 mm (tap), with the first three classified as resistant and tap water as sensitive. The tap water results were significantly different (p<0.05) from those of the other antibiotics. Conclusion The use of non-standard solvents in MHA preparation, particularly tap water, may lead to inconsistent antibiotic susceptibility results. Standardized use of distilled water is recommended to ensure test accuracy and reliability.
Co-Authors Dermawan, Asep Dicki Bakhtiar Purkon Faizah Min Fadhlillah Habibah, Firda Nurul Hotimah, Alni Khusnul Iis Kurniati Indrasari, Witri Lestari, Desi Maya Mamat Rahmat Merdekawati, Fusvita Mimin Kusmiyati, Mimin Nainggolan, Aurelya Veny Kurnia Ningsih, Padmasari Diah Ayu Kumala Nur Indra, Asep Iin Prananda, Crystian Dwi rohayati rohayati Solihat, Mohamad Firman Srinati, Ine Sulaeman Sulaeman Syafitri, Andita Izmi Yayat Sudaryat Zuri Rismiarti