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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Kesehatan Medika Saintika Biosel: Biologi Science and Education BioEksakta : Jurnal Ilmiah Biologi Unsoed
Kurniadi, Helman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary Other

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Isolasi dan Karakterisasi Bakteri Penghasil Enzim β-CGTase dari Tanah Perkebunan Ubi Jalar Kurniadi, Helman; Sagita, Desi; Hartesi, Barmi; Andriani, Lili; Dahlia, Dimbi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 3 (2024): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.3.11779

Abstract

β-CGTase (β-cyclodextrin glycosyl transferase) is an enzyme that converts starch into CD (cyclodextrin). The CD is a high-value oligosaccharide used for modifying food, textiles, chemicals, medicines, cosmetics, and biotechnology. This research aims to obtain isolates of β-CGTase producing bacteria from sweet potato plantation soil. Screening is carried out using Horikoshi media which contains the color indicators phenolphthalein and methyl orange. Bacteria producing β-CGTase will give a yellow hollow zone if they grow on this media. CGTase activity was measured using a UV-Vis spectrophotometer. Three isolates producing CGTase were obtained, coded BSP6A, BSP6B, and BSP6C, which had the characteristics of being Gram-positive, shaped like bacilli, produced spores, were motile, had a positive catalase test, and could ferment acid. These three isolates were able to produce the optimum CGTase enzyme if incubated for 18 and 24 hours at a temperature of 37°C and pH 10 and were able to convert starch into CD if incubated for 30 minutes at a temperature of 37°C and pH 7.
Isolation of b-Cyclodextrin Gycosyl Transferase (b-CGTase) Producing Bacteria from Potato Plantation Soil Kurniadi, Helman; Sagita, Desi; Hartesi, Barmi
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.193-197

Abstract

Cyclodextrin glycosyltransferase (CGTase) is an extracellular enzyme produced by several microorganisms, particularly bacteria, that converts starch into cyclodextrin (CD). CD has various applications in fields such as food, textiles, pharmaceuticals, chemistry, and biotechnology. The objective of this study was to isolate b-CGTase-producing bacteria from soil in a potato plantation located in Kerinci, and to conduct their characterization. Screening was conducted using Horikoshi agar media with phenolphthalein and methyl orange color indicators. The results indicated that one isolate, coded CK-2, produced the optimum b-CGTase enzyme when incubated for 30 hours at 37 ºC and pH 10. The CGTase enzyme converted starch to CD when incubated for 60 minutes at 37 ºC and pH 7. The CK-2 isolate was identified as monobacilli Gram-positive bacteria, having spores, positive in the catalase test, and motile. The study concluded that the bacteria producing b-CGTase enzyme are found in the soil of potato plantations in Kerinci, which is believed to originate from the genus Bacillus sp.
Implementasi Program Penanggulangan Stunting di Dinas Kesehatan Kabupaten Kerinci Kurniadi, Helman; Aghadiati, Faradina; Ardianto, Oril
Jurnal Kesehatan Medika Saintika Vol 15, No 2 (2024): Desember 2024
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v15i2.2921

Abstract

Stunting adalah masalah gizi akibat kekurangan asupan jangka panjang, terutama dari masa kehamilan hingga anak berusia 24 bulan. Penelitian ini menganalisis implementasi program penanggulangan stunting di Dinas Kesehatan Kabupaten Kerinci, menggunakan pendekatan deskriptif kualitatif dan studi kasus. Sampel penelitian dipilih secara purposive sampling, melibatkan petugas kesehatan dan masyarakat yang terkait langsung dengan program ini. Data diperoleh melalui wawancara mendalam dan dianalisis secara tematik dengan triangulasi untuk memastikan validitas. Hasil menunjukkan bahwa koordinasi antarinstansi dalam penanggulangan stunting berjalan efektif dengan dukungan komitmen yang kuat. Terdapat 724 kasus stunting pada anak usia 0-59 bulan, atau sekitar 4,24%, dengan prevalensi tertinggi di Kecamatan Kuliling Danau mencapai 11,80%. Inovasi dalam program penanggulangan stunting telah diterapkan dan mendapatkan respon positif dari masyarakat, meski partisipasi masih perlu ditingkatkan. Implementasi program telah optimal, namun terdapat beberapa tantangan, seperti rendahnya pengetahuan dan kondisi ekonomi kelompok sasaran, keterbatasan anggaran, serta kurangnya publikasi dan konvergensi lintas sektor.
Analisis Fitokimia terhadap Senyawa Aktif dalam Daun Carica Papaya sebagai Dasar Formulasi Kapsul Kurniadi, Helman; Syafitri, Ulfah Eka; Alfiana, Dessinta; Framisa, Mentari; Hairani; Heldiana, Fitria
Biosel Biology Science and Education Vol. 14 No. 1 (2025): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v14i1.9101

Abstract

Papaya leaves (Carica papaya L.) are rich in secondary metabolites with pharmacological potential as antioxidants, anti-inflammatories, and antimicrobials. This study aimed to identify bioactive compounds in papaya leaves, develop simplicia and extracts as a basis for capsule formulation, and evaluate the characteristics and stability of the resulting capsules. The methods used included simplicia preparation, extraction using maceration with 70% ethanol, phytochemical analysis using specific reagent tests, and capsule formulation with appropriate excipients. The results showed that papaya leaf simplicia had a moisture content of ≤ 5%, extraction yielded a rendement of 9.6%, and active compounds such as alkaloids, flavonoids, and polyphenols were detected. Capsule formulations containing papaya leaf extract showed good characteristics, with a dissolution time reaching 80% within 30 minutes, and good stability during storage. Papaya leaf extract can be developed as a stable, effective, and pharmaceutically standardized herbal phytopharmaceutical capsule preparation to support natural medicine. Keywords: C. papaya, Secondary Metabolites, Maceration, Herbal Capsule, Phytochemistry
Co-Authors Alfiana, Dessinta Barmi Hartesi Dahlia, Dimbi Desi Sagita Faradina Aghadiati Framisa, Mentari Hairani Heldiana, Fitria Lili Andriani Oril Ardianto Ros Sumarny Syafitri, Ulfah Eka Syamsudin Abdillah Timbul Partogi Haposan Simorangkir