Article Per Year (5 Year)
p-Index From 2021 - 2026
0.983
P-Index
This Author published in this journals
All Journal Biology, Medicine, & Natural Product Chemistry Sciences of Pharmacy
Okokon, Jude Efiom
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Health Professions Medicine & Pharmacology Public Health

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Effect of Justicia insularis Leaf Extract and Fractions on Oxidative Stress Markers, Liver Function Parameters and Liver Histology of Plasmodium berghei -Infected Mice Enyiekere, Veronica James; Anagboso, Martin Osita; Ise, Uduak Peter; Essien, Grace Emmanuel; Okokon, Jude Efiom; Ebong, Nwakaego Omonigho
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.351-359

Abstract

Justicia insularis (Family-Acanthaceae) is used in Ibibio ethnomedicine to treat malaria. The leaf extract and fractions of J. insularis were investigated for antioxidative stress and hepatoprotective activities in Plasmodium berghei-infected mice. The leaf extract (100-300 mg/kg, p.o.) exerted significant (p<0.05) antimalarial activity against P. berghei infection in curative test with ethyl acetate fraction demonstrating the highest activity.  The extract/fractions treatment caused significant (p<0.05) reductions in liver enzymes (ALT, AST and ALP), total and conjugated bilirubin of the treated infected mice and also decreased significantly (p<0.05) total protein and albumin levels of the treated mice relative to control. The leaf extract and fractions further improved significantly (p<0.05) the levels of oxidative stress markers enzymes and molecules (CAT, GPx, GST, SOD) of the treated infected mice with no significant (p>0.05) effect on GSH. The MDA levels in the livers of the treated infected mice were significantly (p<0.05) reduced relative to control. Histology of liver sections revealed absence or significant reductions in pathological features in infected mice treated with leaf extract (100 mg/kg), DCM and ethyl acetate fractions compared to untreated infected mice. These results suggest that the leaf extract/fractions of Justicia insularis possess antioxidative stress and hepatoprotective potentials, which is an added advantage to its antimalarial property.
In vivo Alpha-amylase and Alpha-glucosidase Inhibitory Potentials of Panicum maximum Jacq. (Guinea grass) Leaf Extract on Wister Rats Enin, Godwin Ndarake; Okokon, Jude Efiom; David, Enobong Mfon; Emmanuel, Saviour Elisha; Ekanem, Esther Michael; Antia, Bassey Sunday
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.681-685

Abstract

Panicum maximum Jacq. (Guinea grass) a medicinal plant used traditionally in the treatment of diseases including diabetes was evaluated for its effect on alpha amylase and alpha glucosidase enzymes in vivo. The crude ethanol extracts (150, 300 and 450 mg/kg) of P. maximum were investigated using starch, sucrose, glucose and maltose as substrates and acarbose as reference drug. The leaf extract caused significant (p<0.05) reduction in blood glucose levels of the treated rats with the four substrates used. The findings show that the leaf extract of Panicum maximum has the potentials to inhibit alpha amylase and alpha glucosidase in rats.
Genotoxic and Cytotoxic Activities of Cornhusk Extract of Zea mays and Leaf Extract of Sacharum officinarum Akpan, Emem Eyo; Anagboso, Martin Osita; Johnny, Imoh Imeh; Ebong, Nwakaego Omonigho; Okokon, Jude Efiom
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.531-540

Abstract

Zea mays husk and Saccharum officinarum have been used for years in ethnomedicine for their antimalarial, anti-inflammatory, antipyretic, antidiabetic, and antiphlogistic activities. The cytotoxic and genotoxic effects of Zea mays husk and Saccharum officinarum leaf extracts on the root meristem cells of Allium cepa were investigated. Onion bulbs were exposed to 2.5 mg/ml, 5mg/ml, and 10 mg/ml concentrations of the extracts for macroscopic and microscopic analysis. Tap water was used as a negative control and Methotrexate (0.1 mg/ml) was used as a positive control. There was statistically significant (p < 0.05) inhibition of root growth depending on concentration by the extracts when compared with the negative control group. All the tested extracts were observed to have cytotoxic effects on cell division in A. cepa. The extract induced chromosomal aberrations and micronuclei (MNC) formations in A. cepa root tip cells were significant (p<0.05) when compared with the control group. The extracts treatment further induced cell death, ghost cells, cells membrane damage, and binucleated cells. The Zea mays husk extract was found to exhibit higher cytotoxic and genotoxic potential than Saccharum officinarum leaf extract. These results suggest that Zea mays husk and Saccharum officinarum leaf extracts possess cytotoxic and genotoxic effects on A. cepa.
Evaluation of Antidiabetic Activity of Croton zambesicus Root Extract: In Vivo Inhibitory Effect on Alpha Amylase and Alpha Glucosidase of Rats Okokon, Jude Efiom; Edem, Utibe Anietie; OSIGWE, Chinyelu Clementina; Uwaeme, Ugonma Florence
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.529-533

Abstract

Diabetes mellitus is a global health challenge, necessitating the need for alternative treatments. Croton zambesicus Muell Arg. (Euphorbiaceace) a medicinal plant used traditionally in the treatment of some ailments was investigated for its inhibitory potential on alpha-amylase and alpha-glucosidase enzymes in rats. The root extract of C. zambesicus (27 - 81 mg/kg) was investigated in vivo for the inhibitory effect on alpha amylase and alpha glucosidase enzymes using starch, sucrose, and maltose as substrates. Acarbose was used as a reference drug. Blood glucose levels (BGL) of rats, post administration of the substrate and extract concurrently, were monitored over 3 hours as a parameter to measure the inhibitory potential of the extract. The root extract dose-dependently caused significant (p<0.05) reduction in blood glucose levels of treated rats with the various substrates used. The results suggest that the root extract of Croton zambesicus has the potentials to inhibit alpha amylase and alpha glucosidase in rats.
Antioxidant Properties, α-Amylase and α-Glucosidase Inhibitory Activities of Maesobotrya barteri Leaves Extracts in Rats Enin, Godwin Ndarake; Ita, Basil Nse; Thomas, Paul Sunday; Okokon, Jude Efiom; Lawson, Blessing Ofonime; Ohanaka, Chidera Getrude
Sciences of Pharmacy Volume 4 Issue 1
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0401294

Abstract

Maesobotrya barteri is widely used in Nigerian ethnomedicine to treat diabetes, arthritis, and infections. In this study, the methanol and aqueous leaf extracts' phytochemical constituents and antioxidant potentials were evaluated using standard procedures. At the same time, the enzyme inhibitory activity of methanol extract on α-amylase and α-glucosidase in rats was also investigated. The antioxidant properties of the extracts were evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing power (FRAP). Phytochemical screening of methanol and aqueous extracts revealed the presence of flavonoids, alkaloids, saponins, tannins, and cardiac glycosides. Total phenolics and flavonoids of the extracts ranged from 0.04 to 3.18 mg of GAE/g and from 27.70 to 57.70 mg of QE/g, respectively. Antioxidant analysis showed IC50 values of DPPH (192.95, 196.04, and 17.19 µg/mL) and FRAP (30.48, 37.64, and 38.15 µg/mL) for the methanolic extract, aqueous extract, and ascorbic acid, respectively. Assessment of the methanolic extract against α-amylase and α-glucosidase enzymes in rats at doses of 150, 300, and 450 mg/kg using starch, sucrose, and maltose as substrates, with acarbose as a reference drug, significantly reduced blood glucose levels (p < 0.05). These findings suggest that M. barteri leaf extract has antioxidant properties and inhibits both α-amylase and α-glucosidase enzymes in rats, likely due to the phytochemicals present in the extract.
Co-Authors Akpan, Emem Eyo Anagboso, Martin Osita Antia, Bassey Sunday David, Enobong Mfon Ebong, Nwakaego Omonigho Edem, Utibe Anietie Ekanem, Esther Michael Emmanuel, Saviour Elisha Enin, Godwin Ndarake Enyiekere, Veronica James Essien, Grace Emmanuel Ise, Uduak Peter Ita, Basil Nse Johnny, Imoh Imeh Lawson, Blessing Ofonime Ohanaka, Chidera Getrude OSIGWE, Chinyelu Clementina Thomas, Paul Sunday Uwaeme, Ugonma Florence