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All Journal Jurnal Ilmu Dasar Biology, Medicine, & Natural Product Chemistry Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) BIOSFER : Jurnal Biologi dan Pendidikan Biologi Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Abdi Insani Journal of Tropical Ethnobiology Soeropati: Journal of Community Service Jurnal Penelitian Sains dan Pendidikan (JPSP) Rumphius Pattimura Biological Journal Proceeding of International Conference on Biology Education, Natural Science, and Technology
Putri, Intan Kurnia
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Antibacterial Bioactivity Test of Bilimbi Fruit Ethanol Extract (Averrhoa bilimbi Linn). Against Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus Anindita, Reza; Nathalia, Dede Dwi; Perwitasari, Melania; Putri, Intan Kurnia; Beandrade, Maya Uzia; Harahap, Nofria Rizki Amalia
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.173-182

Abstract

One of the causes of acnes is the bacteria Propionibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus. An alternative solution to this problem is to develop natural medicines from plants, one of which is the star fruit (Averrhoa bilimbi Linn). The purpose of this study was to test the bioactivity of star fruit against the growth of P. acnes, S. epidermidis, and S. aureus. This research method used an experimental research design with the treatment of A. bilimbi fruit ethanol extract concentrations of 50%, 60%, 70%, 80%, and 90%, chloramphenicol antibiotics as control (+), and sterile distilled water as control (-). All treatments were given to P. acnes, S. epidermidis, and S. aureus bacteria using the Kirby Baeur (disc diffusion) method. The results of this study were the effectiveness of the bioactivity compound of the ethanolic extract of A. bilimbi fruit seen on P. acnes and S. epidermidis bacteria with concentrations of 70%, 80%, and 90%. Thus, it can be concluded that A. bilimbi fruit has the potential to be developed as a natural medicine for acne caused by P. acnes and S. epidermidis.
Anti-Bacterial Activity of Etanolic Extract and Essential Oil of Basil (Ocimum sanctum) on Growth Staphylococcus aureus Salmonella thypii and Eschericia coli Perwitasari, Melania; Anindita, Reza; Beandrade, Maya Uzia; Nathalia, Dede Dwi; Hasmar, Wahyu Nuraini; Putri, Intan Kurnia
Jurnal ILMU DASAR Vol 24 No 2 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i2.31367

Abstract

Antimicrobial resistance is one of the biggest health problems. Indonesia is a country with a positive high number of E. coli (71%) Extended Spectrum Beta Lactamase (ESBL) in the Asia Pacific. Many studies report that essential oils and extracts from several species of Ocimum have antimicrobial activity against gram-positive and negative bacteria. Antibacterial activity is related to active compounds contained in plants that are also affected by the place of growth, harvesting and post-harvesting. Research related to the activity of basil (Ocimum sanctum) that already exists does not provide complete and comprehensive data related to where to grow, harvest and post-harvest basil leaves. Basil leaves that have been set parameters for growth, harvest and post-harvest processes are extracted using maceration method with 70% etanol solvent and distilled to obtain essential oils (EO). Etanolic extract (EE) and EO were tested for their activity against Escherichia coli, Staphylococcus aureus and Salmonella thypii bacteria. The results showed that the basil leaves obtained from Margahayu sub-district, East Bekasi, had a dry simplicia yield of 12.8% ± 1.5, yield of EE 16.9% ± 1.6, EO content of 0.56%. The etanolic extract of basil leaves contains alkaloids, saponins, tannins, phenolics, flavonoids, glycosides, triterpenoids and steroids. The conclusion from the results of this study is the etanol extract and essential oils of basil leaves can inhibit the growth of bacteria, both basile leaves. The inhibition zone diameter is higher in gram positive bacteria (S. aureus) than gram negative bacteria (S. thypii and E. coli).
Community Knowledge about Medicinal Plants in Samudra Jaya and Pahlawan Setia Villages, North Bekasi Anindita, Reza; Haediningrum, Syahla; Beandrade, Maya Uzia; Putri, Intan Kurnia; Nathalia, Dede Dwi; Perwitasari, Melania; Harahap, Nofria Rizki Amalia; Rahmadi, Chandra
Journal of Tropical Ethnobiology Vol. 7 No. 1 (2024): January 2024
Publisher : The Ethnobiological Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this study was to describe the knowledge and use of medicinal plants by the people of Samudra Jaya and Pahlawan Setia Villages, Tarumajaya Sub-district, North Bekasi. The research design is an exploratory survey and observation. The sample numbered 119 people, consisting of 69 people representing Pahlawan Setia Village and 50 people representing Samudra Jaya Village. The sampling technique used purposive random sampling. Variables in this study include the value of community knowledge about medicinal plants, the value of community knowledge about the use of medicinal plants, the organs of plants that are often used, the percentage of how to use medicinal plants, and the families and species of plants used as medicine. Data analysis was carried out descriptively. The results showed that the value of community knowledge about medicinal plants was 354 (classified as good), the value of community knowledge regarding the use of medicinal plants was 300.4 (classified as good), the organs of plants that were often used as medicine were leaves (95%), the percentage of how to use medicinal plants are more dominant by boiling (91%), there are 19 families with 22 species of plants used as medicine. The family includes Zingiberaceae, Polygonaceae, Liliaceae, Acanthaceae, Oxalidaceae, Talinaceae, Crassulaceae, Rubiaceae, Lamiaceae, Piperaceae, Basellaceae, Euphorbiaceae, Solanaceae, Caricaceae, Menispermaceae, Asparagaceae, Zingiberaceae, Myrtaceae, Rutaceae, Leguminosae, and Moraceae, with plant species: kunyit (Curcuma domestica), jambu biji (Psidium guajava), sirih hijau (Piper betle), mengkudu (Morinda citrifolia), lidah buaya (Aloe vera), brotowali (Tinospora crispa), sambiloto (Andrographis paniculata), saga (Abrus precatorius), jahe (Zingiber officinale), belimbing wuluh (Averrhoa bilimbi), cocor bebek (Kalanchoe pinnata), ciplukan (Physalis peruviana), jeruk nipis (Citrus aurantiifolia), jarak pagar (Jatropa curcas), kumis kucing (Orthosiphon aristatus), gondola (Basella rubhalin), pepaya (Carica papaya), ginseng jawa (Talinum paniculatum), telang (Clitoria ternatea), getih-getihan (Rivina humilis), tin (Ficus carica), and suji (Dracaena angustifolia). The study concludes that the results of survey scores regarding the knowledge and use of medicinal plants by the people of Samudra Jaya and Pahlawan Setia Villages are classified as good. The species of medicinal plants that are most widely used are the Zingiberaceae family, with local names of plant species, namely jahe and kunyit. Both plants are used by boiling to relieve menstrual pain and treat bloating and colds.
Formulation and Effectiveness Test of Red Ginger (Zingiber officinale var. Rubrum) and Starfruit (Averrhoa bilimbi L.) Combination Shampoo Against Pediculus humanus capitis Anindita, Reza; Harahap, Salwa Zhafarina; Beandrade, Maya Uzia; Putri, Intan Kurnia
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.565-573

Abstract

Some of the natural ingredients that have the potential to be developed into a health product for pediculosis are red ginger (Zingiber officinale var. Rubrum) and star fruit (Averrhoa bilimbi L.). Secondary metabolite compounds in red ginger extract and star fruit that can potentially kill head lice Pediculosis humanus capitis include alkaloids, flavonoids, and saponins. The purpose of this study was to determine the physical fitness and effectiveness of the lethal power of the combination of red ginger extract and star fruit with formula 1 (F1) 1.5%, formula 2 (F2) 2%, and Formula 3 (F3) 2.5%. The tests carried out were phytochemical screening tests (alkaloids, tannins, saponins, and flavonoids), physical stability of shampoo (organoleptic, homogeneity, foam height, viscosity, and pH) for four weeks of storage, and testing the effectiveness of shampoo killing power against head lice Pediculus humanus capitis. Data analysis was carried out descriptively and comparatively using one-way ANOVA. Phytochemical screening of red ginger extract and star fruit was positive for alkaloids, tannins, saponins, and flavonoids. The organoleptic test of the shampoo on all three formulas produced a light brown colour, a distinctive aroma of lemon oil, and a thick shape. The homogeneity test showed a homogeneous shampoo preparation. F1 foam level test (7.7-11 cm), F2 (7.3-11 cm), and F3 (7.2-11 cm). F1 viscosity test (2,010-2,020 cps); F2 and F3 (2,020 cps). One-way ANOVA pH F1 test (8.54-8.77); F2 (8.01-8.70); F3 (8.61-8.77) and One-way ANOVA results for pH values obtained significant values of 0.096 > 0.05, while the killing power of aphids was 0.02 < 0.05. The three shampoo formulas of red ginger extract and star fruit showed good physical evaluation and still entered the test requirement range for four weeks of storage. Based on the results of statistical analysis, it was shown that the variation in the concentration of the formula did not cause a change in pH during four weeks of storage. The administration of a combination shampoo of red ginger extract and star fruit with a formulation of F1 1.5%, F2 2%, and F3 2.5% was able to have a natural effect on the average number of head lice deaths of Pediculus humanus capitis.
Bioprospecting Test of Piper betle Leaf Essential Oil Against Staphylococcus Aureus and Escherichia Coli-Antibiotic Resistant Anindita, Reza; Beandrade, Maya Uzia; Putri, Intan Kurnia
Biosfer : Jurnal Biologi dan Pendidikan Biologi Vol. 9 No. 2 (2024): BIOSFER: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Unpas,

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/biosfer.v9i2.19303

Abstract

One of the efforts to overcome antibiotic resistance is the bioprospection of natural materials that have the potential to be antibacterial. One of the results of distilling natural materials with antibacterial potential is betel leaf essential oil (Piper betle). This study aimed to determine the effect of essential oils on Staphylococcus aureus and Escherichia coli-resistant antibiotics. The design of this study is experimental, with independent variables in the form of essential oils with concentrations of 5%, 10%, 15%, 20%, 25%, and 100%. In contrast, the dependent variable is the diameter of the growth inhibition zone of S. aureus and E.coli bacteria. Essential oil extraction using steam distillation and chemical characterization by GC-MS test. Antibacterial testing uses the Kirb-Bauer method. GC-MS test results were dominant eugenol (3.50%), caryophyllene (3.79%), and Germacrene D (1.19%). The application of essential oils was able to inhibit the growth of S. aureus with inhibition zone diameters of 10.5 mm (moderate), 10.6 mm (moderate), 10.8 mm (moderate), 12.3 mm (strong), 14 mm (strong), and 30.2 mm (very strong), while E. coli was 5.7 mm (moderate), 7.5 mm (moderate), 10.5 mm (moderate), 12 mm (strong), and 30.03 mm (very strong). The results of the ANOVA one-way test showed a value of 11762.7, while the F table was 3.48 (Fcal > Table). This study concludes that administering essential oils with concentrations of 5%, 10%, 15%, 20%, 25%, and 100% can significantly inhibit the growth of S. aureus and E.coli bacteria.
Botanical, Pharmacognosy, and Phytochemical Studies of Sintrong Leaves (Crassocephalum crepidioides) Growing in Tanjung Setia Village, West Coast, Lampung: Kajian Botani, Farmakognosi, dan Fitokimia Daun Sintrong (Crassocephalum crepidioides) yang Tumbuh di Desa Tanjung Setia Pesisir Barat Lampung Anindita, Reza; Pratama, Gita Cahya; Putri, Intan Kurnia; Beandrade, Maya Uzia; Futihat, Chilyati Eky
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 1 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v12i1.423

Abstract

Crassochepalum crepidioides plant has antibacterial and anti-wound properties. This study aims to determine the botanical profile, microscopic powder, phytochemical screening, and Thin Layer Chromatography (TLC). This study aims to determine the morphology of leaves, stems, roots, microscopic powder, phytochemical screening, and Thin Layer Chromatography (TLC) of C. crepidioides leaves from Tanjung Setia Village West Coast Lampung, Indonesia. Methods include morphological examination, microscopic powder, phytochemical screening, and TLC. The results of the botanical show oval-shaped leaves, pointed tips, pinnate veins, serrated edges, smooth leaf surface, soft wood stems, and tap roots. Microscopic observation found fragments of stomata. Positive phytochemical screening of alkaloids, flavonoids, saponins, and tannins. TLC test spot with five spots (red) and an average Rf value of 0.21-0.97. Concludes that the thin layer chromatography test is thought to produce flavonoid spots at an Rf value of 0.72-0.77.
Training on Making and Testing the Efficacy of Hand Sanitizers from Lime and Sweet Orange Essential Oils for Pharmacy Students at Al-Muhtadin Vocational School Bekasi, West Java, Indonesia Anindita, Reza; Putri, Intan Kurnia; Beandrade, Maya Uzia; Arianti, Anisa Rachmita; Futihat, Chilyati Eky
Jurnal Soeropati Vol 7 No 2: May 2025
Publisher : LPPM Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/js.v7i2.6124

Abstract

Community analysis shows that Al-Muhtadin Pharmacy Vocational School, Bekasi students are interested in making pharmaceutical products from natural ingredients. However, their desires are often hampered by the lack of knowledge regarding manufacturing pharmaceutical preparations from natural ingredients. Therefore, the solution is to hold training in hand sanitizers with natural ingredients from essential oils of lime and sweet orange peels. This Community Service activity (CSA) aims to provide training in making and testing hand sanitizers for Al-Muhtadin Pharmacy Vocational School students in Bekasi. The CSA participants numbered 62 students of Al-Muhtadin Pharmacy Vocational School, Bekasi. The activity methods include providing material on Clean and Healthy Living Behavior (CHLB) and making hand sanitizers, training in making and testing hand sanitizer products, and evaluation in the form of concept tests (pre-test and post-test) and skills tests. The paired t-test results showed that the calculated t-value was smaller than the t-table (-4.952 <-2.013), or there was a significant difference in the average value of knowledge about CHLB and making hand sanitizers between before and after being given the material. In contrast, the practical exam showed the average percentage value of participants' skills in making hand sanitizers of 90.57%, while the efficacy testing skills were 93.44%. Testing of the antibacterial efficacy of hand sanitizer products by respondents showed that they were able to inhibit and kill bacteria on the hands. This CSA concludes that all participants have learned the concept and skills regarding manufacturing and testing hand sanitizers from a combination of lime and sweet orange peel essential oils.
Botany, Pharmacognosy, and Phytochemical Study of Gymnanthemum amygdalina (Delile) Sch.Bip. Leaves from Bandung, West Java, Indonesia Anindita, Reza; Tahapary, Manuela Esterlita; Nathalia, Dede Dwi; Putri, Intan Kurnia; Beandrade, Maya Uzia
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p138-147

Abstract

Gymnanthemum amygdalina (Delile) Sch.Bip. possesses antioxidant, antibacterial, anticholesterol, anticancer, and antidiabetic properties. This study aimed to determine the morphology of leaves, stems, flowers, and roots, as well as the microscopic powder characteristics, phytochemical profile, and Thin Layer Chromatography (TLC) pattern of G. amygdalina leaves. The samples were collected from Bandung City, West Java, Indonesia. Methods included morphological examination, microscopic powder analysis, phytochemical screening, and TLC. The morphological characterization of G. amygdalina showed that the leaves measured 10–22 cm in length and 2–8 cm in width, with an oblong shape, acuminate tips, acute bases, pinnate venation, serrated margins, rough surfaces, woody stems, and a taproot system. Microscopic examination revealed fragments of trichomes. Phytochemical screening indicated the presence of alkaloids, flavonoids, saponins, tannins, and terpenoids/steroids. TLC analysis showed five red spots with an average Rf value ranging from 0.06 to 0.58. In conclusion, the morphological characteristics of G. amygdalina leaves were identified, microscopic analysis revealed trichome fragments, and phytochemical screening confirmed the presence of secondary metabolites. TLC analysis yielded Rf values between 0.06 and 0.58.
Uji Nanoemulsi Ecoenzym Terhadap Bakteri Staphylococcus aureus anindita, Reza; Nathalia, Dede Dwi; Beandrade, Maya Uzia; Putri, Intan Kurnia; Sari, Afrinia Eka; Setyodewi, Elisabeth
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.553

Abstract

The discovery of eco enzyme as a fermented liquid from organic vegetable and fruit waste has been used as an organic plant fertilizer, disinfectant, hand sanitizer, antiseptic soap, and domestic waste degradation agent. However, trials of eco enzymes as raw materials for health preparations have not yet been carried out. Seeing this, trial research is needed on the use of ecoenzyme nanoemulsion against Staphylococcus aureus bacteria. This research aims to determine the antibacterial ability of ecoenzyme nanoemulsion against S. aureus bacteria. The design of this research is experimental. The samples used were ecoenzyme nanoemulsions with Formula 1 (F1) 20%, Formula 2 (F2) 25%, and Formula 3 (F3) 30%. This research includes making ecoenzyme, making ecoenzyme nanoemulsion, testing ecoenzyme nanoparticles, and testing ecoenezyme nanoemulsion against S. aureus bacteria using the Kirby-Baeur method. The results showed that the sizes of ecoenzyme nanoparticles in F1 20%, F2 25%, and F3 30% were 38.9 nm, 65.07 nm, and 146.2 nm, respectively. The results of the antibacterial ability test of nanoemulsion eco enzyme F1 20%, F2 25%, and F3 30% against S. aureus produced an inhibitory zone 4.3 mm (resistant), 4.3 mm (resistant), and 6.7 mm (resistant). This research concludes that the eco enzyme nanoemulsion is not effective in inhibiting the growth of S. aureus bacteria.
Monitoring The Correlation of Climatics to The Airborne Bacteria at The Manggarai Station, South Jakarta, Indonesia Anindita, Reza; Rahmawati, Auliya; Inggraini, Maulin; Perwitasari, Melania; Nathalia, Dede Dwi; Beandrade, Maya Uzia; Putri, Intan Kurnia
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The highest of microorganisms suspended in the air were bacteria with a percentage of 80.8%. If the intensity of bacterial exposure occurs in large quantities, it will accumulate in the respiratory tract which has the potential to trigger allergic reactions and respiratory infections. Given the problems and impacts caused by air microorganisms on public health, it is necessary to monitor the distribution of air bacteria. The purpose of this study was to determine the relationship between temperature and humidity with the number of bacteria in the Manggarai station area. Type of research is correlation. The sampling location is Manggarai Station, East Jakarta, Indonesia. The 10 sampling points in this study include the station hall, motorbike parking, prayer rooms, train platform 1-2, train platform 2-3, train platform 3-4, and train platform 5, toilet, and underpass. The results of this study included the highest number of bacteria found in the station hall 331 colonies, the highest percentage of bacterial morphology is monobacilli was 78%, the distribution of gram positive was more than gram negative with spore bacteria being more dominant than non spore. The correlation coefficient between temperature and number of bacterial colonies (0.134) and humidity with number of bacterial colonies (0.380) showed weak positive correlation, while the correlation coefficient for light intensity with the number of bacterial colonies -0.140 (very weak negative).
Co-Authors Afrinia Ekasari Arianti, Anisa Rachmita Auliya Rahmawati, Auliya Beandrade, Maya Uzia Dwi Rini Kurnia Fitri Futihat, Chilyati Eky Haediningrum, Syahla Harahap, Nofria Rizki Amalia Harahap, Salwa Zhafarina Hasmar, Wahyu Nuraini Inggraini, Maulin Marta, Fhatya Mulyana Melania Perwitasari, Melania Nasution, Dwi Suci Ramadani Nathalia, Dede Dwi Pratama, Gita Cahya Putri, Zulgusma Aulia Rahmadi, Chandra Rianza, Alfitra Selvia, Gusti Ayu Setyodewi, Elisabeth Tahapary, Manuela Esterlita