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All Journal El-Hayah : Jurnal Biologi Organisms: Journal of Biosciences
Kurniawati, Yeyen
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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Identification of Visual Character of Leaves with Sumatran Rhino's Urine in the Sumatran Rhino Sanctuary Way Kambas National Park as Potential Source of eDNA Priyambodo, Priyambodo; Putri, Chicka R.P.; Rustiati, Elly L.; Kurniawati, Yeyen; Zulkarnain, Danisworo; Pratiwi, Dian N.; Arsan, Zulfi; Giyono, Giyono; Mustikawati, Ganis; Pertiwi, Vindo R.; Sukatmoko, Sukatmoko; Srihanto, Eko A.; Saswiyanti, Enny
El-Hayah:Jurnal Biologi Vol 9, No 2 (2023): EL-HAYAH (VOL 9, NO 2 Maret 2023)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v9i2.21179

Abstract

The sumatran rhinoceros is one of the large endemic mammals to the island of Sumatra, with critically endangered status by the IUCN. Currently, the existence of the sumatran rhino is challenging to find in its natural habitat. Therefore, it is necessary to develop indirect monitoring to maintain and increase the stability of the population in nature. Monitoring through camera traps has been used in several locations. Another method is carried out by identifying traces and remains of the sumatran rhino in natural habitats, for example, through urine samples. The sumatran rhinoceros has the behavior of urinating, namely through urine spray. Urine sprayed by the sumatran rhino will hit plant parts in its habitat, including leaves. This study was aimed to identify the visual characteristics of leaves containing samples of sumatran rhino urination as a potential source of eDNA material. The results showed the leaves characteristics containing the sumatran rhino's urine on the 0, 1, and 2 days after the sumatran rhino urination. The difference in visual observations of the sumatran rhino's urine spray attached to the leaves can be seen through the change in the color of the urine fluid, which is getting more and more concentrated. Visualization of leaves containing sumatran rhino urine can be used to recommend visual leaf characteristics that can be taken as similar samples in natural habitats.
Detection of Environmental DNA from Tree Bark Associated with Rubbing Behavior of Dicerorhinus sumatrensis in Way Kambas National Park Priyambodo; Zulkarnain, Danisworo; Rustiati, Elly Lestari; Kurniawati, Yeyen; Putri, Chicka Refina Rahma; Srihanto, Eko Agus; Saswiyanti, Enny; Arsan, Zulfi; Sukatmoko; Pratiwi, Dian Neli; Permatasari, Nindy
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/zv2m2645

Abstract

The development of non-invasive methods is essential for monitoring critically endangered species such as the Dicerorhinus sumatrensis. This study aimed to evaluate the potential of tree bark associated with rubbing behavior as a source of environmental DNA (eDNA) for species detection in Way Kambas National Park. A total of 30 samples were collected from tree bark surfaces showing evidence of rubbing activity. DNA was extracted and analyzed using two approaches: simple electrophoresis and polymerase chain reaction (PCR)-based molecular methods. The results showed that the simple method failed to detect visible DNA bands, indicating low DNA concentration and degradation in environmental samples. In contrast, PCR amplification successfully detected DNA in 5 out of 30 samples, including hair-derived and epithelial residues associated with rubbing behavior.containing hair residues produced more reliable detection compared to those with only epithelial traces. These findings demonstrate that tree bark able to serve as a potential non-invasive source of eDNA, particularly when analyzed using sensitive molecular techniques. This approach provides an alternative method for detecting elusive species and contributes to the development of effective conservation strategies for the sumatran rhinoceros.
Co-Authors Arsan, Zulfi Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Giyono Giyono Mustikawati, Ganis Permatasari, Nindy Pertiwi, Vindo R. Pratiwi, Dian N. Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Putri, Chicka R.P. Putri, Chicka Refina Rahma Rustiati, Elly L. Saswiyanti, Enny Srihanto, Eko A. Sukatmoko Sukatmoko, Sukatmoko Zulkarnain, Danisworo