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All Journal Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Jurnal Kedokteran Brawijaya Global Medical and Health Communication MEDIC MEdical Dedication
Humaryanto, Humaryanto
Jambi University
Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health

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COMPARISON OF THREE METHOD OF DNA EXTRACTION FOR METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) Hanina Hanina; Humaryanto Humaryanto
Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan Vol. 10 No. 4 (2022): Jambi Medical Journal: Jurnal Kedokteran dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.363 KB) | DOI: 10.22437/jmj.v10i4.21047

Abstract

Background: MRSA is a gram positive bacteria that has a peptidoglycan in the cell wall which is noteasy to lysis during the DNA extraction procces.Objectives: This study aimed to compare three DNA extraction method, using a conventional method, chelex method, and commercial kit in MRSA.Methods: This is a descriptive study with laboratory experiments. The sample was swabs of purulent wounds from inpatients in Jambi that were randomly selected. Swab sample identified as MRSA was extracted with three methods and tested using UV-Vis spectrophotometer.Results: The study results showed that the best DNA purity was found by DNA extraction usingcommercial kit (1.43), followed by chelex (1.14) and conventional (1.07) methods. Likewise, the highest concentration of DNA was DNA extraction using commercial kit (330 g/ml), followed by the chelex (78 g/ml) and conventional (54 g/ml) methods.Conclusion: A conventional method can be used as an extraction method that is easy to do and more economical. The best DNA purity and concentration was obtained in the kit extraction method, followed by chelex and conventional methods.Keywords: MRSA, extraction, conventional, chelex, kit
Co-Authors Ahmad Syauqy Anati Purwakanthi, Anati Asro Hayani Harahap Aurora Wahyu Indah Dwi Ave Olivia Rahman dewi, hasna Erny Kusdiyah Fairuz Quzwain Hanina Hanina Hanina, Hanina Huntari Harahap M. Nurhalim Shahib Miftahurrahmah Miftahurrahmah, Miftahurrahmah Nucki Nursjamsi Hidayat Patrick Patrick Patrick William Gading Putri Sari Wulandari Simanjuntak, Charles Apul Tarawifa, Susan Wahyu Indah Dewi Aurora Yoni Fuadah Syukriani