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Effect of Guava Extract Administration on Megakaryocytes Amount in Mice Femur Atik, Nur; Munawir, Maqrizi D. S.; Tarawifa, Susan; Darmadji, Hartati P.
Indonesian Journal of Clinical Pharmacy Vol 6, No 2 (2017)
Publisher : Indonesian Journal of Clinical Pharmacy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1010.183 KB) | DOI: 10.15416/ijcp.2017.6.2.116

Abstract

Dengue fever is a disease spread by mosquito’s bite. Dengue fever is marked by the presence of thrombocytopenia. Traditional crops such as guava are commonly used to treat dengue fever. This research aims to know the effect of guava extract administration towards megakaryocytes amount in mice femur. The study was conducted at the Laboratory of Pharmacology and Therapy, Histology Laboratory of Faculty of Medicine at Universitas Padjadjaran, Eijkman, Bandung from September until November 2016 using laboratory experimental study design. 20 Swiss webster mice strains were divided randomly into 4 groups. Group I and II were administered quinine 2.8 mg/20 grBW/day for 14 days to decrease amount of trombocytes. Group II and III were administered guava extract 0.785 mg/20 grBW/day for 5 days. Group IV was administered aquadest for 19 days. In the 27th day, the mice left femurs were collected and made into paraffin section preparations with hematoxylin-eosin staining and then observed under microscope. Group IV had the most megakaryocytes followed by Group II, III, and I. Based on Kruskal-Wallis test, a significant difference was shown (p<0.05). Mann- Whitney test showed that there were significant differences between Group I and Group II, III, and IV. Meanwhile there was no significant difference between normal mice and extract-given mice. Guava extract is proven statistically significant to increase the megakaryocytes amount in thrombocytopenic mice without increasing number of megakaryocytes in normal mice.
Effect of Guava Extract Administration on Megakaryocytes Amount in Mice Femur Nur Atik; Maqrizi D. S. Munawir; Susan Tarawifa; Hartati P. Darmadji
Indonesian Journal of Clinical Pharmacy Vol 6, No 2 (2017)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3052.478 KB) | DOI: 10.15416/ijcp.2017.6.2.116

Abstract

Dengue fever is a disease spread by mosquito’s bite. Dengue fever is marked by the presence of thrombocytopenia. Traditional crops such as guava are commonly used to treat dengue fever. This research aims to know the effect of guava extract administration towards megakaryocytes amount in mice femur. The study was conducted at the Laboratory of Pharmacology and Therapy, Histology Laboratory of Faculty of Medicine at Universitas Padjadjaran, Eijkman, Bandung from September until November 2016 using laboratory experimental study design. 20 Swiss webster mice strains were divided randomly into 4 groups. Group I and II were administered quinine 2.8 mg/20 grBW/day for 14 days to decrease amount of trombocytes. Group II and III were administered guava extract 0.785 mg/20 grBW/day for 5 days. Group IV was administered aquadest for 19 days. In the 27th day, the mice left femurs were collected and made into paraffin section preparations with hematoxylin-eosin staining and then observed under microscope. Group IV had the most megakaryocytes followed by Group II, III, and I. Based on Kruskal-Wallis test, a significant difference was shown (p<0.05). Mann-Whitney test showed that there were significant differences between Group I and Group II, III, and IV. Meanwhile there was no significant difference between normal mice and extract-given mice. Guava extract is proven statistically significant to increase the megakaryocytes amount in thrombocytopenic mice without increasing number of megakaryocytes in normal mice.Keywords: Dengue fever, guava extract, megakaryocyte, quinine Efek Pemberian Ekstrak Jambu Biji terhadap Jumlah Megakariosit pada Femur MencitAbstrakDemam berdarah merupakan penyakit yang dapat disebarkan melalui gigitan nyamuk. Demam berdarah ini ditandai dengan adanya trombositopenia. Tanaman tradisional berupa jambu biji banyak digunakan untuk mengobati demam berdarah. Penelitian ini bertujuan untuk mengetahui efek pemberian ekstrak jambu biji terhadap jumlah megakariosit pada femur mencit. Penelitian ini dilakukan di Laboratorium Farmakologi dan Terapi, Laboratorium Histologi Universitas Padjadjaran, Eijkman, Bandung dari bulan September hingga November 2016. Sebanyak 20 ekor mencit galur Swiss webster dibagi menjadi 4 kelompok. Kelompok I dan II diberikan quinine 2,8 mg/20 grBB/hari per oral selama 14 hari agar mencit trombositopenia. Kelompok II dan III diberikan ekstrak jambu biji 0,785 mg/20 grBB/hari per oral selama 5 hari. Kelompok IV diberikan aquades selama 19 hari. Hari ke 27, femur mencit kiri diambil dan dijadikan sediaan parafin dengan pewarnaan hematoxylin-eosin, kemudian diamati di bawah mikroskop. Jumlah megakariosit terbanyak adalah pada Kelompok IV diikuti dengan II, III dan I. Berdasarkan uji Kruskal-Wallis, terdapat perbedaan bermakna (p<0,05) dilanjutkan uji Mann-Whitney yaitu terdapat perbedaan bermakna antara Kelompok I dengan II, III, dan IV, sedangkan perbandingan pada kelompok mencit normal dengan kelompok yang diberi ekstrak tidak terdapat perbedaan yang bermakna. Ekstrak jambu biji dapat meningkatkan jumlah megakariosit pada mencit yang mengalami trombositopenia dan tidak menyebabkan peningkatan jumlah megakariosit secara bermakna pada mencit normal.Kata kunci: Demam berdarah, ekstrak jambu biji, megakariosit, quinine
Genetic variant of TGF-ß associated with decreased renal function in type II diabetes mellitus patient: single center pilot study in Indonesia Anggelia Puspasari; Elfiani Elfiani; Susan Tarawifa; Rina Nofri Enis; Asro Hayani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 53, No 4 (2021)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/JMedSci005304202101

Abstract

The interaction between genetic factors, blood glucose and hypertension plays a role in the onset of diabetic kidney disease (DKD) in type II diabetes mellitus (T2DM). Genetic variation of TGF-ß1 is associated with renal complication in T2DM with varying results between ethnicities. The Jambi Malay ethnic, which is the majority ethnic in Jambi Province, is an area that reports an increased prevalence of T2DM with DKD as the most frequent microvascular complications. In addition, previous study reported controlling blood glucose not associated with DKD indicating genetic may have play a role in DKD in this population. Studies related to genetic variation and decreased kidney function in T2DM patients has never been performed in this ethnic group. This study aimed to investigate the role of TGF-ß genetic variation as risk factor for decreased renal function in T2DM patients from Jambi Malay ethnicity. We conducted a cross sectional study involving 70 patients with T2DM. The inclusion criteria for renal complication based on a decrease in the glomerular filtration rate (GFR) of less than 60 mL/min/1.73. The genotyping method used was amplification refractory mutation system polymerase chain reaction (ARMS-PCR) for TGF-β1 rs1800470 T/C. Bivariate and multivariate analysis was performed to analyze phenotype and genotype association. The result of bivariate analysis showed T2DM patients with genotype CT (p:0.006; OR:0.125; 95% CI:0.027-0.575) and CC (p:0.007; OR:0.104; 95% CI:0.020-0.546) or C allele carrier (p:0.003; OR:0.117; 95% CI:0.027-0.500) had lower risk for decreased renal function than TT genotype. Multivariate analysis that included blood pressure and age variables showed the same finding for CT (p:0.007; OR:0.086; 95% CI:0.014-0.508) and CC genotype (p:0.022; OR:0.115; 95% CI:0.018-0.731). It is concluded from this study that T2DM patients with genotype CT, CC and carrier allele C have a lower risk for suffering kidney complications than genotype TT.
The Development of the Basic Obstetric Ultrasound Learning Media for Undergraduate Medical Students HERLAMBANG HERLAMBANG; AMELIA DWI FITRI; NYIMAS NATASHA AYU SHAFIRA; ELFIANI ELFIANI; ANGGELIA PUSPASARI; SUSAN TARAWIFA
Indonesian Research Journal in Education |IRJE| Vol. 4, No. 1, June 2020
Publisher : Universitas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.485 KB) | DOI: 10.22437/irje.v4i1.9423

Abstract

Current medical education curriculum refers to the Indonesian Medical Doctor’s Standard of Competence 2012. Competencies are detailed in seven general areas of competence to be achieved by medical students. This study focused on the development of learning media in the form of guide book and video related to the basic obstetric ultrasound examination and its influence in improving student’s knowledge of basic obstetric examination skills. A quantitative method used to see the effectiveness of the learning media that we developed. Twenty-one under graduate medical students who undertook clinical rotation in the Department of Obstetrics and Gynecology participated in this study through completing the pre-post student’s questionnaires to assess the students' knowledge before and after the delivery of learning media. The collected data were analyzed with a computer program; Means and Standard Deviation were calculated as descriptive parameters. Parametric tests were used to test the hypotheses. The pre-post results were compared using the Wilcoxon statistical test. The results showed a significant difference in the score of pretest and posttest. There was an increase in the average score of pretest and posttest for students after obtaining the teaching media and clinical supervision. The value of each individual was also increased. The analysis with the Wilcoxon statistical test showed significant differences which mean that there were improvements on student’s knowledge of basic obstetric examination skills after the delivery of guide book and video. The use of teaching media in the form of basic obstetric ultrasound module and video contributed to positive results to improve the student’s knowledge.
The Role of Clinical Supervision: Teaching Basic Obstetric Ultrasound for Undergraduate Medical Students Herlambang Herlambang; Amelia Dwi Fitri; Nyimas Natasha Ayu Shafira; Anggelia Puspasari; Susan Tarawifa
Indonesian Research Journal in Education |IRJE| Vol. 4, No. 2, Dec 2020
Publisher : Universitas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.01 KB) | DOI: 10.22437/irje.v4i2.12204

Abstract

This study aimed to find the impact of clinical supervision on the achievement of medical student competence in basic obstetric ultrasound examination skills by using DOPS as the assessment method. Students’ knowledge related to basic obstetric ultrasound examination in the beginning and in the end of their 10 weeks clinical rotation in Department of Obstetrics and Gynecology were assessed by using pre-test and post-test questionnaire. By employing mixed methods, one group pre and post-test design was positioned to assess student knowledge, one group post-test design to assess student’s skills achievement and qualitative study to explore student perception about the new learning method we offered. The collected data were analyzed with computer program; means and standard deviation were calculated as descriptive parameters. Parametric test were used to test the hypothesis. The pre-post results were compared by using Wilcoxon test. The results showed a significant difference in the score of pre-test and post-test. Qualitative data showed positive perception. Clinical supervision as teaching method together with learning media in the form of module and ultrasound video gave positive result to improve achievement of student’s competence in basic obstetric ultrasound examination skills.
IDENTIFIKASI KASUS TOKSOPLASMOSIS DENGAN UJI AGLUTINASI LATEK DI PUSKESMAS TAHTUL YAMAN Humaryanto Humaryanto; Hanina Hanina; Susan Tarawifa
Medical Dedication (medic) : Jurnal Pengabdian kepada Masyarakat FKIK UNJA Vol. 3 No. 1 (2020): MEDIC: Medical Dedication
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/medicaldedication.v3i1.8579

Abstract

Infeksi Toxoplasma gondii dapat terjadi karena perolehan maupun kongenital, yang sering kali tidak menimbulkan gejala yang jelas atau sub klinis sehingga kebanyakan tidak disadari. Pada wanita yang terinfeksi Toksoplasma gondii, selama kehamilan dapat menyebabkan janin yang dikandung mengalami keguguran atau bayi lahir namun mengalami beberapa gangguan baik cacat fisik maupun nonfisik. Di Puskesmas Tahtul Yaman belum ada yang terdeteksi menderita Toksoplasmosis. Hal ini dapat dimengerti mengingat Toksoplasmosis umumnya tidak menimbulkan gejala. Dengan uji aglutinasi latek ini, diharapkan dapat diketahui angka kejadian Toksoplasmosis dan dapat ditindak lanjuti. Metode kegiatan yang dilakukan adalah berupa pemeriksaan darah terhadap 60 orang wanita usia subur dengan menggunakan uji aglutinasi latek. Hasil pemeriksaan menunjukkan mununjukkan terdapat 36 orang positif toxoplasmosis, 20 orang negatif dan 2 orang equivocal. Hasil positif toxoplasmosis paling banyak terdapat pada kelompok usia 30 sampai 39 tahun yaitu sebanyak 28 orang. Pada kelompok wanita yang sudah menikah terdapat 33 orang positif toxoplasmosis dan pada wanita yang pernah mengalami riwayat keguguran terdapat 22 orang positif toxoplasmosis
PENYULUHAN POSISI DUDUK YANG BENAR UNTUK KESEHATAN PUNGGUNG BAGI MASYARAKAT AWAM Mirna Marhami Iskandar; Fairuz Quzwain; Patrick Wiliam Gading; Susan Tarawifa
Medical Dedication (medic) : Jurnal Pengabdian kepada Masyarakat FKIK UNJA Vol. 3 No. 2 (2020): MEDIC: Medical Dedication
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/medicaldedication.v3i2.12536

Abstract

ABSTRAK Latar belakang. Postur tubuh yang kurang tepat dan posisi yang tidak ergonomis saat duduk dapat menyebabkan peningkatan jumlah energi, dan transfer tenaga yang tidak efisien dari otot ke jaringan rangka yang menyebabkan timbulnya kelelahan. Hal ini dapat berujung pada nyeri punggung bawah. Penderita LBP akan mengalami penurunan produktivitas kerja dan disabilitas. Oleh karena itu, perlu dilakukan edukasi kepada para masyarakat awam mengenai postur yang baik untuk mencegah terjadinya LBP. Metode. Penyuluhan diawali dengan pembuatan video edukatif mengenai posisi duduk yang baik, kemudian ditayangkan melalui webinar daring, di samping adanya materi dari narasumber ahli. Penyuluhan juga mencakup tanya-jawab dengan responden. Hasil. Didapatkan 69 orang responden di mana sebagian besarnya berusia produktif dewasa muda-dewasa yaitu 21-40 (88,39%), berjenis kelamin perempuan (69,57%), berasal dari kota Jambi (49,28%), dengan pekerjaan mahasiswa (36,23%) atau karyawan swasta (17,39%). Nyeri punggung pernah diderita oleh 92% dari responden, dan didapat pula 69% telah mengetahui bahwa postur tubuh dan posisi duduk dapat menyebabkan nyeri punggung namun baru 54% yang mengetahui ciri postur tubuh dan posisi duduk yang baik. Kesimpulan. Mayoritas masyarakat awam usia produktif pernah mengalami nyeri punggung selama hidupnya, memahami bahwa postur dan posisi duduk dapat menyebabkan nyeri punggung, namun belum semuanya mengetahui ciri postur dan posisi duduk yang baik untuk pencegahan nyeri punggung. Penyuluhan awam secara daring dapat menjadi suatu alternatif yang efektif dalam tindakan preventif bagi nyeri punggung bawah dengan cara meningkatkan pengetahuan masyarakat awam dengan suasana yang interaktif. Kata Kunci : Low back pain, nyeri punggung, posisi duduk, postur, ergonomis, penyuluhan daring
PENYULUHAN KESEHATAN MENJAGA ANAK SEHAT DAN BAHAGIA DI MASA PANDEMI COVID-19 Nindya Aryanty; Attiya Istarini; Ima Maria; Susan Tarawifa; Adelina Fitri
Medical Dedication (medic) : Jurnal Pengabdian kepada Masyarakat FKIK UNJA Vol. 4 No. 2 (2021): MEDIC. Medical dedication
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACT The COVID-19 pandemic has had an impact on learning methods, namely from face-to-face to distance learning. Digital devices can provide highly sophisticated learning opportunities for early childhood. However, in conditions of activity restrictions during the pandemic, people have a tendency to use digital devices as a means of education, entertainment, and social interaction, which means that they also increase the duration of screen time in their daily lives. According to the American Academy of Child and Adolescent Psychiatry (AACAP), the maximum screen time for toddlers is 1 hour per day. Excessive screen time in children has been shown to be associated with behavioral health problems. The level of parental knowledge regarding the allowed screen time duration is quite good. This can be seen from the survey results where, after giving the material, respondents who have children in the 3-5 year age group who know the screen time duration is less than 1 hour are around 84.7% of respondents, where before receiving the material only 63.4% of respondents . Meanwhile, for respondents who have children aged 6-12 years as much as 77.1% know screen time after getting the material, where previously only 63.9%. It can be concluded that the implementation of this counseling was able to increase the knowledge of parents about the impact of excessive use of gadgets so as to prevent the adverse effects of gadgets such as negative behavior, obesity, and internet or gadget addiction. In addition, it is necessary to conduct counseling on the adverse effects of using gadgets in the adolescent and adult age groups. Keywords: COVID-19, Child Health, Screen Time ABSTRAK Pandemi COVID-19 memiliki dampak pada metode pembelajaran yaitu dari tatap muka menjadi pembelajaran jarak jauh. Perangkat digital dapat memberikan peluang belajar yang sangat canggih bagi anak usia dini. Namun dalam kondisi pembatasan aktivitas selama pandemi, masyarakat memiliki kecenderungan untuk menggunakan perangkat digital sebagai sarana pendidikan, hiburan, dan interaksi sosial, yang artinya turut meningkatkan durasi screen time dalam keseharian. Menurut American Academy of Child and Adolescent Psychiatry (AACAP), durasi screen time pada balita maksimal 1 jam per hari. Durasi screen time yang berlebihan pada anak-anak telah diketahui memiliki asosiasi terhadap permasalahan perilaku kesehatan. Tingkat pengetahuan orang tua mengenai durasi screen time yang diperbolehkan sudah cukup baik. Ini terlihat dari hasil survey dimana, setelah pemberian materi, pada responden yang memiliki anak di kelompok usia 3-5 tahun yang mengetahui durasi screen time kurang dari 1 jam adalah sekitar 84,7% responden, dimana sebelum mendapat materi hanya 63,4% responden. Sedangkan untuk responden yang memiliki anak usia 6-12 tahun sebanyak 77,1% mengetahui screen time setelah mendapatkan materi, dimana sebelumnya hanya 63,9%. Dapat disimpulkan pelaksanaan penyuluhan ini ternyata dapat meningkatkan pengetahuan para orang tua mengenai dampak penggunaan gadget berlebihan sehingga mencegah terjadinya efek buruk gadget seperti perilaku negatif, obesitas, dan kecanduan internet atau gadget. Selain itu perlu dilakukan penyuluhan dampak buruk penggunaan gadget pada kelompok usia remaja dan dewasa. Kata Kunci: COVID-19, Kesehatan Anak, Screen Time
HUBUNGAN KADAR HBA1C DENGAN RESIKO NEFROPATI DIABETIKUM PADA PASIEN DM TIPE 2 DI RSUD H. ABDUL MANAP KOTA JAMBI Susan Tarawifa; Samuel Batara Bonar; Imanuel Sitepu
Jurnal Ilmu Kedokteran dan Kesehatan Vol 7, No 2 (2020): Volume 7 Nomor 2
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.223 KB) | DOI: 10.33024/jikk.v7i2.2755

Abstract

Hiperglikemia kronis pada diabetes mellitus tipe 2 berhubungan dengan disfungsi atau kegagalan beberapa organ tubuh seperti ginjal (nefropati diabetikum). Pengendalian hiperglikemia pada penderita DM tipe 2 sangat penting dan tidak boleh diabaikan karena berhubungan dengan morbiditas dan mortalitas penderita DM tipe 2 tersebut. HbA1c merupakan salah satu pemeriksaan status glikemik pada penderita DM tipe 2 dan pemeriksaan mikroalbuminuria berguna untuk pemantauan dini resiko nefropati diabetikum. Penelitian ini bertujuan untuk mengetahui hubungan kadar HbA1c dengan Resiko Nefropati Diabetikum pada pasien DM tipe 2 di RSUD H. Abdul Manap Kota Jambi. Penelitian ini menggunakan metode analitik cross-sectional dengan menggunakan teknik consecutive sampling sesuai dengan kriteria inklusi. Analisis data dilakukan univariat dan bivariate menggunakan uji Chi-Square. Dari hasil 30 subjek penelitian, didapatkan pasien dengan usia 56-65 sebesar 66, jenis kelamin perempuan sebesar 66,7% perempuan. Hasil kadar HbA1c tidak terkontrol didapatkan hasil 50% beresiko nefropati diabetikum dan 50% normal. Hasil kadar HbA1c yang terkontrol didapatkan hasil 62,5% normal dan 37,5% beresiko nefropati diabetikum Hasil uji statistik menunjukkan bahwa nilai p-value=0,42 yang berarti tidak adanya hubungan yang bermakna antara kadar HbA1c dengan resiko nefropati diabetikum pada pasien DM tipe 2 di RSUD H. Abdul Manap Kota Jambi.
PROFIL LUARAN MATERNAL DAN NEONATAL SERTA IDENTIFIKASI EKSPRESI GEN SFLT-1 PADA PASIEN PREEKLAMPSIA DI KOTA JAMBI Herlambang Herlambang; Susan Tarawifa; Rina Nofri Enis; Amelia Dwi Fitri; Ahmad Syauqi
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 5 No. 2 (2021): Oktober 2021
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v5i2.2532

Abstract

Pendahuluan: Preeklampsia merupakan kondisi spesifik pada kehamilan yang ditandai dengan adanya disfungsi plasenta dan respon maternal terhadap adanya inflamasi sistemik dan kerusakan endotel. Diagnosis preeklampsia ditegakkan berdasarkan adanya hipertensi dan proteinuria pada usia kehamilan diatas 20 minggu. Patogenesis preeklampsia sangat kompleks, banyak gen yang ikut terlibat dalam patogenesis preeklampsia, diantaranya adalah SFlt-1 yang disekresikan plasenta dalam keadaan hipoksia. Faktor risiko lain yang juga berpengaruh pada kejadian preeklampsia adalah usia ibu dan status gravida. Kondisi preeklampsia akan berefek kepada kondisi perkembangan janin selama kehamilan, termasuk persalinan prematuritas. Penelitian ini dilakukan untuk mengetahui profil luaran maternal dan neonatal serta ekspresi gen SFlt-1 pada pasien preeklampsia di Kota Jambi.Metode: Penelitian ini adalah penelitian deskriptif observasional menggunakan metode crossectional, yang menjadi sampel pada penelitian ini adalah ibu hamil yang memenuhi kriteria dengan cara pengambilan sampel secara purposive sampling. Ekspresi mRNA yang akan dinilai diekstrak dari jaringan plasenta pasien preeklampsia pada saat terminasi kehamilan. ekspresi gen dinilai secara kuantitatif menggunakan realtime PCR.Hasil: Untuk profil usia ibu pada pasien preeklampsia didapatkan lebih banyak ibu dengan usia berisiko tinggi (52%) dan primigravida (56%) yang mengalami preeklampsia dan, luaran neonatal didapatkan bayi dengan BBLR (28%) serta ekspresi gen sFlt-1 didapatkan ΔΔcq -2.89 pada usia kelahiran, -3.27 pada usia ibu, 0.80 pada status gravida untuk pasien preeklampsia.Kesimpulan: profil usia ibu lebih banyak ibu dengan usia risiko tinggi, kelahiran preterm dan primigravida pada pasien preeklampsia. Pada preeklampsia didapatkan luaran neonatal yaitu BBLR. Ekspresi gen sFlt-1 meningkat pada pasien preeklampsia.