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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Ternak Jurnal Ilmu Nutrisi dan Teknologi Pakan
Fitri, Ainissya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Veterinary

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Daya Cerna dan Karakteristik Fermentasi Rumen dengan Penambahan Asam Amino Terenkapsulasi secara In Vitro: In Vitro Digestibility and Rumen Fermentation Characteristics with the Addition of Encapsulated Amino Acid Talapessy, Cornelia; Rahayuningsih, Mulyorini; Fidriyanto, Rusli; Fitri, Ainissya; Ridwan, Roni
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 22 No. 2 (2024): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.22.2.85-90

Abstract

Amino acid protection is one of the efforts to improve livestock feed quality. Lysine is one of the limiting amino acids, which is easily degraded in the rumen, and thus requires a protection. This study aimed to determine the effect of protected lysine on cattle's digestive system and ruminal ecology in vitro. This study used encapsulation using xanthan gum and tannin (2% w/v) to protect lysine. The treatments consisted of P0 = unprotected amino acids; P1 = amino acids + 2 g xanthan gum; P2 = amino acids + 2 g tannin; P3 = amino acids + 2 g xanthan gum + 2 g tannin. Parameters observed included nutrient content, chemical activity, degradability of organic matter (OM) and crude protein (CP), and rumen fermentability (pH, N-NH3, VFA, gas kinetics, and CH4). A combination of xanthan gum and tannin coatings (P3) resulted in a higher effect (p<0.05) on rumen fermentation compared to others. The Undegraded DM and CP values in the rumen were 48% and 32%, respectively, and in the pepsin-HCl were 15% and 89%. All treatments did not change the rumen fermentability. In conclusion, protected amino acids has a effect on the cattle digestion system without disturbing the rumen ecology. Combining xanthan gum and tannin coatings provides the best results than using a single type of coating. Key words: amino acids, encapsulation, rumen fermentation, tannin, xanthan gum
Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different Diets Diana, Milsa Solva; Fitri, Ainissya; Anja, Meryandini; Ridwan, Roni
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.80-88

Abstract

The Borneo earless monitor lizard (Lanthanotus borneensis) is one of the endemic animals of Indonesia. In 2019, the IUCN listed L. borneensis as an ‘endangered’ species. The declining population of the L. borneensis is a major concern for conservation efforts. One of the efforts to conserve endangered wild animals is to know the gut microbiome profile and the relationship between the host and the bacterial community in the body, which is associated with an influence on health. This research aims to determine the gut microbiome profile of animals with metagenomic analysis using Next-Generation Sequencing (NGS). This research was carried out by providing three feed types: earthworms, shrimp, and fish. Sample feces was collected by cloacal swabs and the DNA was sequenced using the Illumina NGS technique in the V4 16S rRNA region to examine the gut microbiome. The results of the metagenomic analysis showed differences in bacterial abundance in each feed treatment. The L. borneensis treated with earthworms and fish were dominated at the phylum level by Proteobacteria and Bacteriodota, while in shrimp, the phyla were Proteobacteria and Firmicutes. Index Shanon, Simpson, and the rarefaction curve also showed the highest bacterial diversity found in the fish treatment. This study can be used as a reference in designing suitable feed formulations for effective captive breeding for the conservation of L. borneensis
ABUNDANCE OF Succinivibrionaceae BACTERIA IN THE FECES OF CALVES FED TANNINS, AMINO ACIDS, AND THEIR COMBINATION Sitanggang, Fajar Edy Maretno; Fitri, Ainissya; Permana, Idat Galih; Christi, Raden Febrianto
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 25, No 3 (2025)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v25i3.65207

Abstract

The purpose of this study was to determine how common Succinivibrionaceae bacteria are in calf feces. Four female calves, with an average age of 6.48 ± 0.51 months, participated in a Latin square trial featuring four dietary treatments: control (C), tannin (T), amino acid (AA), and a tannin-amino acid mixture (TM). Duration of each treatment was 14 days whereby faecal samples were that were harvested on day 14. The treatment trial for 14 days included the collection of faecal samples on day 14 for the extraction of DNA to conduct Next-Generation Sequencing (NGS) analysis. Results showed that the Succinivibrionaceae population grew extensively when treated with T and AA and TM compared with C while AA was most effective. Analysis of bacteria detected five genera and five species. The bacterial genus Ruminobacter dominated in the C (53%) and AA (62%) and TM (58%) treatment groups while Succinivibrio dominated the T treatment group (78%). The species Ruminobacter amylophilus dominated treatments C, AA, and TM but the species Succinivibrio dextrinsolvens dominated treatment T. The results infer that the addition of tannins or amino acids or their combination to the gut environment may help in reducing methane emissions through an alteration in the microbiome composition.
Co-Authors Anja, Meryandini Christi, Raden Febrianto Diana, Milsa Solva Fidriyanto, Rusli Idat Galih Permana Mulyorini Rahayuningsih RONI RIDWAN Sitanggang, Fajar Edy Maretno Talapessy, Cornelia