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Mulyorini Rahayuningsih
Department Of Agroindustrial Technology, Faculty Of Agricultural Techonology, Bogor Agricultural University, Darmaga Campus, Bogor, 16680
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Transportation

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KARAKTERISTIK KEFIR DENGAN PENAMBAHAN PUREE UMBI GEMBILI Kartika, Kartika; Rahayuningsih, Mulyorini; Setyaningsih, Dwi
EDUFORTECH Vol 4, No 2
Publisher : Program Studi Pendidikan Teknologi Agroindustri, UPI (Universitas Pendidikan Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/edufortech.v4i2.19372

Abstract

Kefir merupakan produk susu yang beraroma asam, alkoholik, karbonat, dan dibuat melalui proses fermentasi menggunakan mikroba bakteria dan yeast. Kefir merupakan probiotik alami yang mengandung bakteri hidup yang dapat memberikan efek menyehatkan bagi tubuh manusia apabila dikonsumsi. Umbi gembili mengandung inulin yang dapat dimanfaatkan sebagai prebiotik. Tujuan dari penelitian ini adalah mengetahui pengaruh penambahan puree gembili dan waktu fermentasi pada nilai pH, total asam tertitrasi, total gula dan gula pereduksi, serta pertumbuhan bakteri asam laktat dan khamir pada kefir. Metode yang digunakan dalam penelitian ini adalah eksperimen dengan analisis data deskriptif. Hasil dari penelitian ini menunjukan bahwa penambahan puree gembili sebanyak 4% dapat menurunkan nilai pH, meningkatkan total asam tertitrasi, meningkatkan total gula dan gula pereduksi, serta meningkatkan pertumbuhan bakteri asam laktat dan khamir dibandingkan kefir kontrol. Lama waktu fermentasi kefir puree gembili terbaik adalah selama 24 jam dengan nilai pH 4,17; total asam tertitrasi 0,64%; bakteri asam laktat 8,991 log CFU/ml dan khamir 8,579 log CFU/ml.
UTILIZATION OF DIETHANOLAMIDE SURFACTANT FROM METHYL ESTERS OF PALM OIL IN HERBICIDE FORMULATION WITH ACTIVE ISOPROPYLAMINE GLYPHOSATE Rusdiana, Ika Agustin; Hambali, Erliza; Rahayuningsih, Mulyorini
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.113.44-53

Abstract

A surfactant that can be developed from palm oil is non-ionic. Diethanolamine is a nonionic surfactant based on palm oil methyl ester, which can replace the use of polyoxyethylene amine surfactant in a commercial herbicide formula that harms the environment. This research aimed to determine the physicochemical properties of diethanolamine surfactant and to study the effect of diethanolamine surfactant addition in herbicide formulation with active ingredients of isopropylamine glyphosate. This study was arranged in a complete randomized design with surfactant concentrations as treatment, consisting of 1%, 2%, 3%, 4%, 5%, 6%, 7%, 4%, 5%, 6%, 7%, 8%, and 9% (w/w). The results showed that there was a significant effect of diethanolamine surfactant addition on the characteristics of the herbicide formulation. The concentration of 5% diethanolamine surfactant had the best character in producing herbicide with the lowest surface tension and a contact angle of 30.73 dyne/cm and 11.48°. The commercial herbicide, having a surface tension of 36.27 dyne/cm and a larger contact angle of 83.03°, was used as the comparison for the formulations. The stability of the solution was up to 100% for 5 weeks at room temperature with solubility in water of 80.60% and droplet size of 7.20 ?m. 
Direct Ethanol Production from Breadfruit Starch (Artocarpus communis Forst.) by Engineered Simultaneous Saccharification and Fermentation (ESSF) using Microbes Consortium Farida, Iftachul; Syamsu, Khaswar; Rahayuningsih, Mulyorini
International Journal of Renewable Energy Development Vol 4, No 1 (2015): February 2015
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.4.1.25-31

Abstract

Breadfruit (Artocarpus communis Forst.) is one of sources for ethanol production, which has high starch content (89%). Ethanol production from breadfruit starch was conducted by Simultaneous Saccharification and Fermentation (SSF) technology using microbes consortium. The aim of the research was to examine a method to produce ethanol by SSF technology using microbes consortium at high yield and efficiency. The main research consisted of two treatments, namely normal SSF and enginereed SSF. The results showed that normal SSF using aeration and agitation during cultivation could produce ethanol at 11.15 ± 0.18 g/L, with the yield of product (Yp/s) 0.34 g ethanol/g substrate; and yield of biomass (Yx/s) 0.29 g cell/g substrate, respectively. A better result was obtained using engineered SSF in which aeration was stopped after biomass condition has reached the end of the exponential phase. The ethanol produced was 12.75 ± 0.04 g/L, with the yields of product (Yp/s) 0.41 g ethanol/g substrate, and the yield of cell (Yx/s) 0.09 g cell/g substrate.
Penggandaan Skala Produksi Bioinsektisida Bacillus thuringiensis var. israelensis untuk Membasmi Jentik Nyamuk Aedes aegypti Mulyorini Rahayuningsih; Khaswar Syamsu; Abdul Aziz Darwis; Rini Purnawati
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.727 KB)

Abstract

The objective of this research is to study the scaling up of bionsecticide production from Bacillus thuringiensisvar. israelensisusing onggok (a cassava by-product) as a carbon source. The insecticide produced was used to eradicate Aedes aegypti larvae. The product was a crystal protein produced during bacterial sporulation. Scaling up from laboratory to pilot plant scale was done using two methods, i.e. constant agitation power per unit volume (Pg/V) and constant oxygen transfer coefficient (kLa). The results showed that yield of product per substrate (Yp/s) of Pg/V based product with the value of 3.52 ± 0.02 spora per gram substrates was higher than Yp/s of  kLa based product with the value of 2.96 spora per gram substrate.  Logarithmic value of viable spore count (log of VSC) was also higher, i.e. 7.23 ± 0.30 for Pg/V based product as compared to 7.17 ± 0.20 for kLa based product. Substrate efficiency was also higher in Pg/V based (92.47%) than kLa based (64.87%). LC50 of Pg/V based product was lower (0.49 μg/ml) meaning that it was more toxic than kLa based product (0.62 μg/ml). Amino acid content of Pg/V based product was also higher than kLa based product. Constant Pg/V method was suggested as a based on the scaling up of bioinsecticide production of B. thuringiensis israe/ensison industrial scale. Keywords: bioinsecticide, Bacillus thuringiensisvar. israelensis, kLa, Pg/V, LC50, viable spore count
MODIFIKASI PENGOLAHAN DURIAN FERMENTASI (TEMPOYAK) DAN PERBAIKAN KEMASAN UNTUK MEMPERTAHANKAN MUTU DAN MEMPERPANJANG UMUR SIMPAN Rapeka Reli, Endang Warsiki, dan Mulyorini Rahayuningsih
Jurnal Teknologi Industri Pertanian Vol. 27 No. 1 (2017): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tempoyakis one of traditional food that has short shelf life. The pasteurization and packaging improvement could be expected can maintain the quality and extend the tempoyak’s shelf life. This research used polyethylene terephtalate (PET) and polyamide as packaging materials; with modified atmosphere packaging (MAP), non MAP, vacuum and non vacuum. The parameters quality of tempoyak were observed by value of pH, sugar content, lactic acid, alcohol, lactic acid bacteria growth and total plate count. The result showed that the best quality of tempoyak was produced by product with pasteurization treatment. Lactobacillus genus with L.plantarum, L. casei, and L. fermentum were the dominant species of lactic acid bacteria. The result also showed that after the tempoyak was fermented during 84 hours, then the pasteurization should be conducted to stop the fermentation. The 84th hours was a constant phase in which that was the right time to inhibit the acid lactic bacteria growth. Pasteurized tempoyak packaged in PET with MAP technique had the longest shelf life as much as 84 days than others treatment whose had aboutonly 14 untill 56 days. The decreasing of tempoyak quality at 84 days was showed by colour change of tempoyak from yelowness to brown, pH decreased from 3.97 to 3.73, lactic acid content decreased from 1.49% to 0.99%, sugar content decreased from 16.57% to 3.28%, alcohol content of 0.32%, number of lactic acid bacteria decreased from 174.3×104cfu/g to 8.6×102 cfu/g, and total plate count from 177×104 cfu/g to 182.3×103 cfu/g.Keywords: modified atmosfir packaging, pasteurization, tempoyak, vacuum
PENGARUH KONSENTRASI ENZIM DAN WAKTU HIDROLISIS ENZIMATIS TERHADAP MUTU FRUKTO-OLIGOSAKARIDA DARI INULIN UMBI DAHLIA (Dahlia pinnata) Djumali Mangunwidjaja, Mulyorini Rahayuningsih dan Reni Suparwati (E-Jurnal Agro-Industri Indonesia)
E-jurnal Agro-Industri Indonesia Vol. 3 No. 2 (2014): E-Jurnal Agroindustri Indonesia
Publisher : E-jurnal Agro-Industri Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.323 KB)

Abstract

ABSTRAK Frukto-oligosakarida  (FOS) merupakan prebiotik yang dapat diperoleh dengan menghidrolisis inulin. Tujuan dari penelitian ini yaitu mendapatkan dan menganalisis kadar inulin, mendapatkan enzim inulinase dan mengukur nilai aktivitas enzimnya, serta mendapatkan FOS dan karakterisasinya. Penelitian ini  dilaksanakan  dalam  dua  tahap,  yaitu  penelitian  pendahuluan  dan  penelitian  utama.  Penelitian pendahuluan meliputi analisis proksimat bahan (umbi dahlia, gembili, bonggol pisang, dan turubuk), ekstraksi tepung inulin, pengujian kadar inulin, produksi enzim inulinase kasar dan pengujian aktivitas enzimnya. Penelitian utama meliputi proses hidrolisis tepung inulin menjadi FOS menggunakan enzim inulinase komersial (dosis 1; 2; 3; 5; 7.5; dan 10 U/g) dan inulinase kasar (dosis 1; 2; dan 3 U/g) selama 24 jam serta pengujian karakteristik FOS. Hasil rendemen tepung inulin tertinggi didapatkan dari umbi dahlia sebesar 48.20% dengan kadar   inulin 80.09%. Nilai aktivitas enzim inulinase inulinase kasar yang dihasilkan sebesar 0.76 U/ml. Pada hidrolisis inulin menggunakan inulinase komersial rata-rata produk yang terbentuk sebagian besar adalah fruktosa.   Pada hidrolisis inulin menggunakan inulinase kasar rata-rata produk yang terbentuk sebagian besar adalah FOS.
Desalination Technique on Seaweeds Hydrolysate Eucheuma Cottonii for Bioethanol Production Jaini Fakhrudin; Dwi Setyaningsih; Mulyorini Rahayuningsih
International Journal of Engineering, Science and Information Technology Vol 1, No 4 (2021)
Publisher : Master Program of Information Technology, Universitas Malikussaleh, Aceh Utara, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.984 KB) | DOI: 10.52088/ijesty.v1i4.191

Abstract

The dissolved salts on Eucheuma cottonii hydrolysate interfere the growth of S. cerevisiae in the fermentation as it is considered as inhibitors. These salts are derived from biomass and formed from the chemicals used for hydrolysis processes such as H2SO4 and CaOH2. Ions and cations of the salts are potential as inhibitors such as Na+, Cl-, NH4+, SO42-. Osmotic pressure is raised due to the presence of salt. The efforts had been made to reduce salinity level through electrodialysis. The objective of this study was to eliminate dissolved salts in the hydrolysate in order to optimize the process of fermentation and increase ethanol yield. The results showed that the process of desalination by electrodialysis was able to reduce the salinity of 20% on the voltage of 5 V for 30 minutes; the voltage 9 V for 15 minutes; the voltage 12 V for 30 minutes. The lowest decline of SO42- occurred at treatment of 12 V for 45 minutes. The content of sulfate in the treatment decreased to 2.97 g/l or 46.22%. The more sulfate is reduced through the electrodialysis process, the better the fermentation process is carried out. Desalination treatment at a voltage of 5 V for 30 minutes had been the best treatment because it    produced the highest ethanol yield of 2.06%. All the voltage treatments and the length of time on the electrodialysis process had a significant influence on the levels of ethanol yield. The process of desalination by electrodialysis was able to reduce the levels of dissolved salts, then it had a significant effect on ethanol yield.
ANALISIS DAUR HIDUP (LIFE CYCLE ASSESSMENT) PENGOLAHAN KOPI BUBUK ROBUSTA SECARA BASAH DI INDUSTRI KECIL MENENGAH (IKM) BELOE KLASIK LAMPUNG Febilian Adiwinata; Suprihatin Suprihatin; Mulyorini Rahayuningsih
AGROINTEK Vol 15, No 4 (2021)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v15i4.11338

Abstract

Indonesia is one of the countries with the largest producer of coffee commodities globally. The high productivity of coffee is a good opportunity to establish Small and Medium Industries for coffee processing. The increase in coffee production will affect the quality of the environment due to the entire series of coffee production activities such as pollution or emissions and waste. The policies set by the government encourage industries to become environmentally friendly and sustainable industries. An assessment of the coffee processing life cycle needs to be carried out to determine how big the environmental impact is from the production process. The magnitude of the environmental impact can be calculated using the Life Cycle Assessment (LCA) method from providing raw materials to products ready for consumption by consumers resulting in environmental impacts and requiring energy that can be calculated. The purpose of this study is to analyze and evaluate the environmental impact of coffee processing production activities using the Life Cycle Assessment (LCA) method with a gate to gate scope. The stages of LCA research are setting research objectives and scope, inventory analysis, environmental impact assessment, and implementation of improvements. The environmental impact analyzed is Greenhouse Gas (GHG) emissions which are categorized as one of the causes of Global Warming Potential (GWP). The results of the LCA analysis show that for every one tonne of wet-processed coffee, 1900 MJ of energy will be used, and GHG emissions of 270.17 Kg CO2eq / day are equivalent to 1.31 CO2eq / kg of ground coffee, which is equivalent to 6,438 Kg CO2eq. / month and 77,260 Kg CO2eq/ year.
PENGOLAHAN LIMBAH CAIR PERIKANAN MENGGUNAKAN KONSORSIUMMIKROBA INDIGENOUS PROTEOLITIK DAN LIPOLITIK Devi Ambarwaty Oktavia; Djumali Mangunwidjaja; Singgih Wibowo; Titi Candra Sunarti; Mulyorini Rahayuningsih
AGROINTEK Vol 6, No 2 (2012)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v6i2.1975

Abstract

Fish processing industries produce liquid waste at different capacity in every level from production line. Because of less attention and knowledge, wastes become worse. The aim of this study was to discover potential microbial consortium that can degrade protein and fat of liquid waste from fish production processing. The consortiums formula were chosen from bacteria that  have the highest specific growth. Formulation A which degraded soluble protein than others. Formulation of B which degraded fat than others. All formulation had pH in range are 6 - 9. Liquid waste which outoclaved had degraded protein, soluble protein and pH parameters
Bioinsecticides Production by Bacillus thuringiensis subsp. aizawai using Agroindustrial by-Product in Solid Fermentation Kirana Sanggrami Sasmitaloka; Titi Candra Sunarti; Mulyorini Rahayuningsih
Jurnal Penelitian Pascapanen Pertanian Vol 13, No 1 (2016): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v13n1.2016.1-10

Abstract

Bacillus thuringiensis subsp. aizawai is one of well-known bioinsectiside resources, and utilized in organic farming for the replacement of chemical insecticide. The active compounds produced from fermentation process of B. thuringiensis subsp. aizawai are bacterial spores and protein crystal of δ-endotoxins, which are variously toxic to larvae of the Lepidoptera and Coleoptera. The use of bioinsecticides in Indonesia is still rarely because bioinsecticide marketed in Indonesia is still an import product so that price is relatively expensive. This problem can be overcome with producing bioinsecticide contain active B. thuringiensis using agroindustrial by product as a raw materials. This research compared the bioinsecticide production from several agroindustrial by product in solid fermentation of B. thuringiensis subsp. aizawai. This research aimed to find out the ability of B. thuringiensis subsp. aizawai using substrate combination of carbon source (cassava dregs, pulp of coffee, starch fractions of iles-iles, and sago dregs) and nitrogen source (tofu’s waste, peanut meal, palm kernel cake, and corn hominy) in bioinsecticides production using solid media cultivation. The research consisted of three stages, there were substrates characterization, microorganisms characterization, and bioinsecticides production. The main parameters to select the carbon and nitrogen sources are LC50 value and potential bioinsecticides products. The results showed that the best cultivation of B. thuringiensis subsp. aizawai contained in combination cassava dregs and palm kernel cake blends. This cultivation produced total cell counts 11.2 log CFU/g, viable spore counts 8.9 log CFU/g, LC50 0.04 μg/ ml and potential products 20000 IU/mg. Biopesticide produced can be used to kill Crocidolomia binotalis on cabbage. PRODUKSI BIOINSEKTISIDA OLEH Bacillus thuringiensis subs. aizawai PADA KULTIVASI MEDIA PADAT MENGGUNAKAN LIMBAH AGROINDUSTRI Bacillus thuringiensis subsp. aizawai merupakan salah satu bakteri yang digunakan untuk menghasilkan bioinsektisida dan digunakan pada pertanian organik untuk menggantikan pemakaian insektisida kimia. Komponen aktif yang diproduksi dari proses fermentasi B. thuringiensis subsp.aizawai adalah spora dan kristal protein δ-endotoksin, yang bersifat toksin terhadap larva Lepidoptera dan Coleoptera. Penggunaan bioinsektisida di Indonesia masih jarang karena biasanya berupa produk impor yang harganya mahal. Permasalahan ini dapat diselesaikan dengan memproduksi bioinsektisida menggunakan bahan aktif B. thuringiensis dan hasil samping agroindustri sebagai bahan baku. Penelitian ini bertujuan mengkaji kemampuan produksi B. thuringiensis subsp. aizawai pada beberapa jenis substrat sumber C (onggok, kulit kopi, fraksi pati iles-iles, dan ela sagu) dan sumber N (ampas tahu, bungkil kacang tanah, bungkil inti sawit, dan ampok jagung) dalam memproduksi bioinsektisida dari limbah agroindustri. Penelitian ini terdiri dari tiga tahap, yaitu karakterisasi substrat, karakterisasi isolat dan produksi bioinsektisida. Parameter utama untuk menetapkan sumber karbon dan nitrogen yang tepat adalah nilai LC50 dan potensi produk bioinsektisida. Hasil penelitian menunjukkan bahwa produksi bioinsektisida yang terbaik terdapat pada kultivasi B. thuringiensis subsp. aizawai menggunakan kombinasi onggok dan bungkil inti sawit. Pada kultivasi ini dihasilkan jumlah sel hidup 11.2 log CFU/g, jumlah spora 8.9 log CFU/g, nilai LC50 0.04 μg/ ml dan potensi bioinsektisida 20000 IU/mg. Bioinsektisida yang dihasilkan dapat digunakan untuk membasmi larva Croccidolomia binotalis pada tanaman kubis.
Co-Authors Abdul Aziz Darwis Ariq Rizky Fadhlurrakhman Ati Atul Quddus Atikana, Akhirta Bambang Arif Nugraha Devi Ambarwaty Oktavia Djumali Mangunwidjaja Dwi Setyaningsih Elisa Anggraeni Erliza Hambali Erliza Noor Febilian Adiwinata Febilian Adiwinata Fidriyanto, Rusli Fitri, Ainissya Iftachul Farida Ika Agustin Rusdiana Ika Agustin Rusdiana Ika Amalia Kartika Jaini Fakhrudin Jesslyn Alvina Alan Kartika Kartika Khaswar Syamsu Kiki Puspita Amalia Kirana Sanggrami Sasmitaloka Michael Silaen Mohamad Yani Muhammad Nur Faidzin Nadhifah, Hana NANIK RAHMANI Neneng Hasanah Ninda Ningtyas NISA RACHMANIA MUBARIK PUSPITA LISDIYANTI Rini Purnawati RONI RIDWAN Rosa, Mutya Rozalia Rozalia Rusdiana, Ika Agustin SHANTI RATNAKOMALA Singgih Wibowo Slamet Budijanto Suprihatin Suprihatin Suprihatin Suprihatin Talapessy, Cornelia Titi Candra Sunarti