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Kontribusi Budidaya Keramba Jaring Apung terhadap Peningkatan Faktor Pembatas Fosfor dan Nitrogen di Ekosistem Waduk Pulau Jawa Prasetya, Indra Sindhu; Nugrayani, Dewi; Safitri, Ayu Putri; Abrilia, Maulita
MAIYAH Vol 2 No 4 (2023): Maiyah : Vol. 2 No. 4 Desember 2023
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.maiyah.2023.2.4.9956

Floating net cage (KJA) cultivation activities have had a significant impact on the community, especially in terms of providing food, increasing employment opportunities and economic growth in the surrounding area. However, we need to realize that KJA has negative impacts that can affect water quality and community activities. Article reviews aims to determine the contribution of phosphorus and nitrogen as limiting factors in reservoir ecosystems due to floating net cages and identify methods that can reduce their negative impacts. KJA activities cause an increase in excessive accumulation of phosphorus and nitrogen in reservoir waters, which comes from food waste and fish metabolism. In addition, the KJA population that fills the reservoir's capacity provides a significant contribution to this increase. The impact of this accumulation is an increased potential for eutrophication, which can damage aquatic ecosystems by causing excessive growth of algae and air plants and decreasing water quality. This is a serious problem that can endanger fisheries cultivation in reservoir waters. Therefore, there are various strategies that can be implemented. This includes implementing stricter regulations regarding KJA practices, educational efforts for cultivators, as well as anti-collaboration between stakeholders (pentahelix). Therefore, the use of cleaning organisms and the use of innovative technology can also be an effective solution in preserving the aquatic environment.

Performa Pertumbuhan Nilem (Osteochilus vittatus) yang Diberi Pakan dengan Suplementasi Garam Syakuri, Hamdan; Pramono, Taufik Budhi; Ekasanti, Anandita; Nugrayani, Dewi; Listiowati, Emyliana
Sainteks Vol 21, No 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v21i1.21069

Suplementasi garam dalam pakan ikan mempunyai peluang meningkatkan efisiensi produksi perikanan budidaya melalui peningkatan pertumbuhan ikan. Tujuan penelitian ini adalah mengkaji performa pertumbuhan Nilem (Osteochilus vittatus) yang diberi pakan dengan suplemen garam. Penelitian eksperimen dilaksanakan menggunakan rancangan acak lengkap, terdiri atas lima perlakuan dan enam ulangan. Perlakuan berupa suplementasi garam dengan dosis 0% (kontrol), 1%, 2%, 3%, dan 4%. Benih ikan (2,3 ± 0,16 g) dipelihara dalam sistem resirkulasi menggunakan wadah berisi 40 liter air dengan kepadatan 15 ekor per wadah dan diberi pakan uji selama 30 hari. Pertumbuhan ikan dan kualitas air diamati secara periodik. Hasil penelitian menunjukkan kelompok ikan yang diberi suplementasi 3% garam memiliki pertumbuhan yang tidak signifikan lebih baik berdasarkan pertambahan berat, relative growth rate (RGR) dan specific growth rate (SGR). Ikan perlakuan 2% garam menunjukkan faktor kondisi yang cenderung lebih tinggi dibandingkan ikan perlakuan lain, namun tidak signifikan secara statistik. Suplementasi garam tidak meningkatkan pertumbuhan ikan secara nyata namun berpotensi dapat membuat produksi budidaya Nilem menjadi lebih efisien.

CULTURE PRODUCTIVITY OF Daphnia magna FED WITH QUAIL DROPPINGS (Coturnix coturnix) Santoso, Marhaendro; Belagama, Ganal Andherpati; Nugrayani, Dewi; Pramono, Taufik Budhi
Journal of Fish Health Vol. 3 No. 1 (2023): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v3i1.2857

This research aimed to know the effect of quail manure on an abundance of Daphnia magna. The research method for D. magna was a Completely Randomized Design (CRD) with four settings, each repeated four times, namely the use of P0 (Control without fertilizer), P1 (Quail manure fertilize 1 g/L), P2 (Quail manure fertilize 3 g/L), P3 (Quail manure fertilize 5 g/L). D. magna was cultured for 15 days in a container with a volume of 3 liters. The updated parameters were abundance of D. magna, mortality of D. magna, and water quality. The results were analyzed using ANOVA analysis. The results showed that the highest abundance was in P2 (3g / L), and the lowest was in P0 (control). Water quality during the research was temperature 21-24Â°C, pH 7,5-8,4, DO 6,2-7,8 ppm, and ammonia 0-0,25 mg/L.

Penilaian Ekonomi Ekosistem Mangrove di Desa Tritih Kulon dan Karangtalun, Kabupaten Cilacap Chairummah, Annis; Wisudyanti, Dewi; Riviani, Riviani; Ekasanti, Anandita; Listiowati, Emyliana; Nugrayani, Dewi; Pelamonia, Alamanda
MAIYAH Vol 3 No 3 (2024): Maiyah : Vol. 3 No. 3 September 2024
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.maiyah.2024.3.3.13144

Mangrove forests function as a place for the survival of various marine biota such as fish, shrimp, and crabs, especially the mangrove forest area in Tritih Kulon and Karangtalun Villages, Cilacap Regency. Mangrove forests in the area also function as a place for the reproduction of living things that can support the economy of the local community. The purpose of this internship is to determine the economic potential of the mangrove ecosystem and to determine the economic impact of the existence of the mangrove ecosystem on the income of local communities in Tritih Kulon and Karangtalun Villages, Cilacap Regency. This internship was carried out by conducting interviews and distributing questionnaires to obtain data. The results of the internship showed that the economic potential of mangrove forests in the Tritih Kulon area is quite high, namely IDR 330,430,000 and IDR 53,700,000 in Karangtalun Village. The existence of mangrove forests in both locations brings economic value to the local community through tourism activities, fisheries, and home industry production. It is hoped that this research can be a reference in the development of mangrove ecosystems in the future.

Inventory of Pests and Parasites in the Cultivation of Hotate Shellfish (Patinopecten yessoensis) in Funka Bay, Hokkaido, Japan Belva Putri Merdeka, Kezia; Ekasanti, Anandita; Nugrayani, Dewi; Matsui Gyogyobu, Daisan
Journal Of Artha Biological Engineering Vol. 2 No. 1 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/0je7xm44

Hotate Shellfish Cultivation is the largest shellfish cultivation in the world, but many pests and parasites are found. This research aims to inventory the types of pests and parasites found in the cultivation of Hotate Shellfish (Patinopecten yessoensis) in Funka Bay, Hokkaido, Japan. The objects used for this research were 54 Hotate shellfish, consisting of three life stages of shellfish, namely seed shellfish, juvenile shellfish, and adult shellfish. This research was carried out using observation and random sampling methods to determine the types and percentages of pests and parasites present during the research. This research was carried out for seven months from 13 April to 6 December 2022 at the Daisan Matsui Company, Ohama, Oshamambe, Hokkaido, Japan. The results of this research show that there are four types of pests, namely Starfish, Purple Shellfish, Crustaceans, and Sea Pineapples as well as two types of parasites, namely Annelid Worms and Barnacles which attack shellfish cultivation. The percentage of shellfish at three life stages that were attacked by parasites was 75.93%. There are several factors that control the attachment of pests and parasites, including substrate availability, nutrient availability, and environmental considerations. The surfaces and nutrients provided by hotate culture facilities provide adequate conditions for pest settlement and growth.

The Effects of Substitution on Fish Meal with Maggot Meal in Artificial Feedfor Eels (Anguilla bicolor) Growth and Survival Rate ahmad, ananda; Sukardi, Purnama; Nugrayani, Dewi; Trianingtyas, Laela
Journal Of Artha Biological Engineering Vol. 3 No. 1 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/fwrbjv08

Eel (Anguilla sp.) is a leading freshwater fish commodity. In the intensivesystem, high stocking density results in high production costs. Maggot meal isan alternative raw material that has complete nutritional value such as fishmeal but is cheaper. The purpose of this study was to determine theeffectiveness of substituted fish meal with maggot meal on the growth andsurvival rate (Anguilla bicolor) Elver phase. The method carried out in thisstudy used an experimental method with a completely randomized design(CRD) consisting of 4 treatments and 3 replications with a combination of fishmeal and maggot meal, P0: 100% TI and 0% TM, P1: 80% TI and 20% TM,P2: TI 60% and TM 40% and P3: TI 40% and TM 60%. The results of rearingfor 60 days showed a significant effect on absolute weight growth, specificgrowth rate, and yield, while the yield had no significant effect on absolutelength growth, feed conversion, and survival. The results of the water qualityparameters of the media are temperatures ranging from 24 °C -29,8 °C, pHranging from 5,14-7,7, DO ranging from 2,4 mg/l – 5,1 mg/l and ammoniaranging from 0 ppm – 0,01 ppm.

GROWTH PERFORMANCE AND INTESTINAL Aeromonas spp. OF BONYLIP BARB (Osteochilus vittatus) AFTER RECEIVING DIET CONTAINING SALT AND HERBAL CONCOCTION SUPPLEMENTATION Syakuri, Hamdan; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Ekasanti, Anandita; Kasprijo, Kasprijo; Kusuma, Rima Oktavia; Wijaya, Rudy; Nugrayani, Dewi; Listiowati, Emyliana; Palupi, Mustika
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.111-122

Salt and herbal supplementations in fish feed are expected to promote aquaculture productivity, including for slow-growing fish such as Bonylip barb (Osteochilus vittatus). The objective of this study was to evaluate the growth performance and occurrence of Aeromonas spp. in the intestine of Bonylip barb fed a diet supplemented with salt and herbs. This experiment was conducted using a completely randomized design with four treatments: 1) control, 2) salt 3% supplementation, 3) herbal supplementation, and 4) Salt 3% and herbal supplementation. Growth performance evaluation included weight gain, relative growth rate, and specific growth rate after 30 and 60 days of rearing. Bacterial samples were isolated on trypticase soy agar (TSA) and glutamate starch phenol red agar (GSP) media. Yellow colonies on GSP were counted and confirmed as Aeromonas spp. via molecular identification based on 16S rDNA sequence. Results showed that herbal supplementation, either alone or in combination with 3% salt, insignificantly increased the fish growth performance in the short-term (30 days), but tended to decrease it over the long-term (60 days). Salt supplementation consistently decreased fish growth performance but improved fish survival rates. Aeromonas isolates comprised more than 60 % of the culturable intestinal bacteria in all treatments. Furthermore, herbal supplementation appeared to increase the percentage of Aeromonas spp. in the intestine, with identified species including Aeromonas veronii, Aeromonas caviae, and Aeromonas jandaei.

ISOLASI, IDENTIFIKASI, DAN ANALISIS EKSPRESI GEN PENGKODE GROWTH HORMONE PADA IKAN SIDAT (Anguilla bicolor) Syakuri, Hamdan; Wibowo, Satrio Haryu; Ekasanti, Anandita; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Listiowati, Emyliana; Nugrayani, Dewi; Sukardi, Purnama
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.69-83

Gen hormon pertumbuhan (growth hormone, GH) perlu dipelajari untuk mendukung domestikasi ikan sidat Anguilla bicolor di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi gen pengkode GH serta menganalisis ekspresinya pada sampel ikan sidat A. bicolor. Primer untuk amplifikasi gen GH ikan sidat A. bicolor didesain berdasarkan sekuen gen GH dari beberapa spesies ikan sidat lain yang tersedia di GenBank. Sampel cDNA hipofisa dan otak ikan sidat digunakan untuk amplifikasi gen GH. Hasil amplifikasi disekuensing dan hasilnya dianalisis menggunakan analisis BLAST, multiple sequences alignment, hormon signature, dan filogenetik. Analisis ekspresi gen GH dilakukan menggunakan teknik realtime PCR dengan metode delta-delta CT pada 14 sampel ikan sidat (23,1 ± 19,6 g; 24,5 ± 4,2 cm). Fragmen DNA sepanjang 486 bp berhasil diamplifikasi dan disekuensing. Sekuen gen GH A. bicolor memiliki similaritas nukleotida sebesar 98,49-99,14% jika dibandingkan dengan gen GH ikan sidat A. anguilla, A. australis, dan A. japonica. Sekuen parsial tersebut secara in silico diketahui mengkode bagian dari protein GH sepanjang 155 asam amino (aa). Sekuen asam amino protein GH A. bicolor sangat mirip dengan sekuen spesies ikan sidat lain dengan hanya tiga perbedaan asam amino dan membentuk satu percabangan pada pohon filogenetik. Tingkat ekspresi gen GH pada sampel ikan sidat memiliki variasi yang tinggi. Hasil penelitian ini dapat menjadi dasar untuk studi selanjutnya khususnya berkaitan dengan peran gen GH dalam pertumbuhan ikan sidat A. bicolor.The growth hormone (GH) gene needs to be studied to support the domestication of the Anguilla bicolor eel in Indonesia. This study aimed to isolate and identify the GH coding gene and analyze its expression in samples of the eel A. bicolor. Primers for amplification of the GH gene of A. bicolor eels were designed based on GH gene sequences from several other eel species available in GenBank. Pituitary and brain cDNA samples of eel were used for GH gene amplification. The amplification results were sequenced and the results were analyzed using BLAST analysis, multiple sequence alignment, hormone signature, and phylogenetic analysis. The GH gene expression analysis was carried out using the real-time PCR technique with the delta-delta CT method on 14 eel samples (23.1 ± 19.6 g; 24.5 ± 4.2 cm). The 486 bp DNA fragment was successfully amplified and sequenced. The GH gene sequence of A. bicolor has a nucleotide similarity of 98.49-99.14% when compared with the GH gene of other eel species A. anguilla, A. australis, and A. japonica. This partial sequence was found in silico to code 155 amino acids (aa) GH protein. The amino acid sequence of the A. bicolor GH protein is very similar to that of other eel species with only three amino acid differences and forms one branch on the phylogenetic tree. The expression level of the GH gene in eel samples had high variations. The results of this study could be a basis for further studies, especially regarding the role of the GH gene in the growth of the eel A. bicolor.

CULTURE PRODUCTIVITY OF Daphnia magna FED WITH QUAIL DROPPINGS (Coturnix coturnix) Santoso, Marhaendro; Belagama, Ganal Andherpati; Nugrayani, Dewi; Pramono, Taufik Budhi
Journal of Fish Health Vol. 3 No. 1 (2023): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v3i1.2857

This research aimed to know the effect of quail manure on an abundance of Daphnia magna. The research method for D. magna was a Completely Randomized Design (CRD) with four settings, each repeated four times, namely the use of P0 (Control without fertilizer), P1 (Quail manure fertilize 1 g/L), P2 (Quail manure fertilize 3 g/L), P3 (Quail manure fertilize 5 g/L). D. magna was cultured for 15 days in a container with a volume of 3 liters. The updated parameters were abundance of D. magna, mortality of D. magna, and water quality. The results were analyzed using ANOVA analysis. The results showed that the highest abundance was in P2 (3g / L), and the lowest was in P0 (control). Water quality during the research was temperature 21-24Â°C, pH 7,5-8,4, DO 6,2-7,8 ppm, and ammonia 0-0,25 mg/L.

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