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All Journal Science and Technology Indonesia Martabe : Jurnal Pengabdian Kepada Masyarakat Sriwijaya Bioscientia Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Indonesian Journal of Applied Environmental Studies
Muharni, .
Department Of Biology, Faculty Of Mathematic And Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Journal : Science and Technology Indonesia

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Optimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology Hary Widjajanti; Elisa Nurnawati; Muharni; Eca Desriana Zahwa
Science and Technology Indonesia Vol. 7 No. 2 (2022): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1560.248 KB) | DOI: 10.26554/sti.2022.7.2.149-157

Abstract

Secondary metabolites extract of McB1 endophytic fungi from gelam (Melaleuca cajuputi Powell.) leaves have a high potential antibacterial activity against Escherichia coli ATCC8739 and Staphylococcus aureus ATCC6538 with flavonoids and phenol as bioactive compound. The low production of secondary metabolites extract in the cultivation stage and the high potential antibacterial activity of bioactive compounds produced by McB1 endophytic fungi require special treatment for optimize the secondary metabolites product. This is possibly achieved by optimizing the composition of the cultivation media, where various sources of carbon, nitrogen, and pH produce different amounts and classes of secondary metabolites. The objectives of the research to obtain the optimum interaction between sources of carbon, nitrogen, and pH for the production of secondary metabolite extract using Response Surface Methodology (RSM). The results showed that the highest extract (0.25 g) with the composition of sucrose as carbon source, yeast extract as nitrogen source, and pH 6. Based on the optimization of the medium with a variation of 4.5 gL-1 sucrose, 0.48 gL-1 yeast extract, and pH 6.1 yielded 0.34 g of secondary metabolites extract of McB1 endophytic fungi. The chromatogram profile of the optimized secondary metabolite extract showed the presence of flavonoids, phenols, terpenoids, and tannins.
Isolation and Molecular Identification of Direct Red 80 Synthetic Dye Degradation Bacteria from Palembang Indonesia Jumputan Cloth Industrial Waste Muharni; Elisa Nurnawati; Heni Yohandini; Hary Widjajanti
Science and Technology Indonesia Vol. 8 No. 3 (2023): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2023.8.3.429-435

Abstract

Industrial waste from Jumputan cloth production pose a significant risk to environmental safety due to their toxic synthetic dye content. Several studies have shown that the presence of bacteria in these materials plays a very important role in decolorization process of the constituent dye. Therefore, this study aims to isolate bacteria with the ability to decolorize direct red 80 from Jumputan cloth industrial waste. Characterization of isolates was carried out macroscopically, microscopically, and biochemically, followed by molecular identification using the 16S rRNA gene. Decolorization effects of the samples on red dye 80 were then assessed using a spectrophotometer at a maximum wavelength of 528 nm. The results showed that 6 bacteria isolates can degrade dye, with decolorizing power ranging from 26.33±0.94 - 73.67±0.47. The highest potential for decolorizing waste synthetic dye is seen in isolate BD 05. Phylogenetic analysis showed that there were 3 genera of bacteria among the samples obtained, namely Bacillus, Aeromonas, and Pseudomonas. These bacteria were closely related to Bacillus tropicus, Areomonas jandaei, and Pseudomonas stutzeri. Pseudomonas stutzeri (BD 05) has the highest potential in handling jumputan industrial waste.
Co-Authors Agus Purwoko Ahmad Aminudin Bama Aminudin Bama, Ahmad Arum Setiawan Arwinsyah Arwinsyah Eca Desriana Zahwa Elisa Nurnawati Endri Junaidi, Endri Erni Erni Erni Erni Fitrya . Fitrya Fitrya Hary Widjajanti Hary Widjajanti Hary Widjajanti Heni Yohandini Indra Yustian Juswardi Juswardi Juswardi Juswardi, Juswardi Laila Hanum Marcella, Maria Mega Tiara Mokhamad Yusup Nur Khakim Mustafa Kamal Nina Tanzerina Nita Aminasih Pratiwi, Putri Rizki Rosmania, Rosmania Salni Sarmayanta Sembiring Sarmayanta Sembiring Syafrina Lamin, Syafrina Triwardana, Singgih Yusup Nur Khakim, Mokhamad