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All Journal HAYATI Journal of Biosciences Pharmaciana: Jurnal Kefarmasian Science and Technology Indonesia
Zahiroh, Fadhilah Haifa
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Physics

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Phytochemical constituent, α-amylase and α-glucosidase inhibitory activities of Black Soybean (Glycine soja (L.) Merr.) ethanol extract Sutjiatmo, Afifah Bambang; Narvikasari, Suci; Solihat, Ananda Khairunisa; Widowati, Wahyu; Kusuma, Hanna Sari Widya; Zahiroh, Fadhilah Haifa
Pharmaciana Vol. 14 No. 3 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i3.29919

Abstract

Diabetes is characterized as a hyperglycemic condition impacted by β-cell dysfunction and insulin deficiency. Black soybean (Glycine soja (L.) Merr.) is widely known as an origin of nutritious food that has shown activities in preventing cardiovascular disease and reducing hyperglycemia. This research aimed to evaluate the potential of black soybeans ethanol extract (BSEE) as an α-amylase and α-glucosidase activity inhibitor. Black soybean seeds were extracted using the Soxhlet method with 50% ethanol as a solvent. The extract Soybean seeds were screened for the presence of phytochemicals. Inhibitory activity of α-amylase and α-glucosidase enzymes was tested in vitro with acarbose as a control. The absorbance measurement was conducted at 565 nm and 400 nm, respectively. BSEE contained alkaloids, flavonoids, polyphenols, saponins, quinones, tannins, and terpenoids. The results indicated that BSEE exhibited a weak inhibitory effect of α-amylase enzyme activity, with an IC50 value of 360.37 ± 20.80 µg/ml, in contrast to acarbose, which showed a significantly lower IC50 of 4.02 ± 0.56 µg/ml. Meanwhile, BSEE was classified as an active inhibitor of α-glucosidase enzyme activity, presenting 25.67 ± 0.27 µg/mL IC50 value, while acarbose demonstrated 10.85 ± 0.5 µg/mL IC50 value. In conclusion, BSEE inhibits α-amylase and α-glucosidase.
Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells Gondokesumo, Marisca Evalina; Novilla, Arina; Prahastuti, Sijani; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Hadiprasetyo, Dhanar Septyawan; Surakusumah, Wahyu
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.228-237

Abstract

Leukemia, characterized by abnormal leukocyte proliferation, ranks ninth in Indonesia as the most common cancer. While treatments such as chemotherapy and radiation effectively target cancer cells, they also risk damaging healthy blood cells. This has spurred interest in exploring low-toxicity herbal compounds as potential therapies, with mangosteen peel emerging as a widely researched option. Nanotechnology, which has the potential to enhance the bioavailability of herbal compounds, is also a focus of extensive research. This study objective was to assess the impact of Mangosteen Peel Nanocrystal (MPN) on HL-60 leukemia cells by analyzing various parameters, including cytotoxicity, reactive oxygen species (ROS) levels, senescence, and gene expression changes. MPN was prepared with high-speed milling and characterized using particle size analyzers, microscopy, and stability assessments. HL-60 cells were cultured and subjected to MPN treatment. Cytotoxicity was evaluated using WST-8 assays, ROS levels were assessed using flow cytometry, and senescence analyses using Senescence-Associated b-Galactosidase Staining. AKT and FLT-1 gene expression were determined via qRT-PCR. MPN has been successfully characterized as a nanoparticle based on size, stability, and morphology. MPN has an impact on leukemia cells by increasing cytotoxicity, decreasing ROS levels, inducing senescence, and modulating AKT and FLT-1 gene expressions. The findings suggest potential implications for MPN in targeting leukemia cells. The study sheds light on the promising effects of MPN in leukemia cell models, indicating its potential applications in targeting cancer cells, inducing senescence, decreasing ROS levels, and modulating gene expressions related to cell survival and proliferation.
The Antiaging Potential of Serum Formulations from Centella asiatica, Curcuma longa, Aloe vera, Rosa centifolia, and Salmon DNA on Injured Human Fibroblast Cells Girsang, Ermi; Wargasetia, Teresa Liliana; Rahmat, Deni; Gondokesumo, Marisca Evalina; Harjanti, Mathelda Weni; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Saufa, Zahra Qisthi; Takasenserang, Oktaviana; Hadiprasetyo, Dhanar Septyawan
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.623-631

Abstract

External aging factors such as UV exposure, pollution, and lifestyle choices contribute to skin aging, resulting in deep wrinkles, fine lines, and rough skin, which can lower self-confidence. Plant extracts have been widely studied for their antiaging potential, while Salmon DNA has shown promise in stimulating collagen production. This study explores the formulation of a serum combining Centella asiatica, Curcuma longa, Aloe vera, Rosa centifolia, and Salmon DNA for its antiaging effects on injured human fibroblast cells. The serum was formulated using extracts from C. asiatica, C. longa, A. vera, R. centifolia, and Salmon DNA. Antioxidant activity was evaluated with the DPPH method, cytotoxicity using the WST-8 assay, and gene expression through qRT-PCR for COL1A1, TGF-β1, HYAL-1, and FGF-2. The serum exhibited weak antioxidant activity (IC50 = 373.33 µg/ml) and reduced cell viability at high concentrations. Gene expression analysis revealed increased expression of COL1A1, TGF-β1, and FGF-2, along with reduced HYAL-1 expression in injured BJ cells. The formulated serum shows potential as an antiaging agent, promoting collagen production and reducing hyaluronidase activity.
Potential of Bitter Melon (Momordica charantia L.) Extract for Chronic Kidney Disease Based on In Vitro Study via TGF/SMADs Signaling, Antioxidant, Antiinflammation, Apoptosis Inducer Activities Prahastuti, Sijani; Rahardja, Fanny; Wargasetia, Teresa Liliana; Zahiroh, Fadhilah Haifa; Sabrina, Adilah Hafizha Nur; Kusuma, Hanna Sari Widya; Azis, Rizal; Hadiprasetyo, Dhanar Septyawan; Ningrum, Siti Ratu Rahayu; Widowati, Wahyu; Sarwono, Sylvie
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.538-551

Abstract

Chronic kidney disease (CKD) is a physiological abnormality in the kidneys whose prevalence is expected to continue to increase. On the other hand, Bitter melon (Momordica charantia L.) is known to have the potential to manage CKD. This study explores the compound content of M. charantia ethanol extract (MCEE) and its potential for CKD based on in vitro assays. To model chronic kidney disease (CKD), SV40 MES-13 (mouse glomerular mesangial) cells were exposed for 3 days to 20 mM glucose. After glucose induction, the cells were subjected with different concentrations of MCEE (Momordica charantia L. ethanolic extract). The chemical profile of MCEE was analyzed using LC/MS-MS. Cell viability was examined through the WST assay, while intracellular ROS and apoptosis levels were measured by flowcytometry. Colorimetry was used to analyze SOD, MDA, and CAT levels. ELISA was used to analyze inflammatory proteins (TGF-β 1, IL-6, TNF-α, IL-1β ) levels. Meanwhile, the relative gene expression of SMAD-2, SMAD-3, SMAD-4, SMAD-7 was examined through qRT-PCR. The results exhibited that MCEE contains cucurbitane p-coumaric, ferulic acid, caffeic acid, gallic acid, chlorogenic acid, and epicatechin. MCEE was also known to be non-toxic to SV40 MES-13 cells. In addition, MCEE reduced intracellular ROS levels, MDA, necrosis levels, and inflammatory proteins, while also regulating SMAD-2, SMAD-3, and SMAD-4 gene expression. MCEE increased levels of CAT, and SOD, and regulated SMAD-7 gene expression in the CKD cells model. The most effective MCEE is MCEE 50 μg/mL. MCEE demonstrated potential as a CKD treatment based on in vitro studies through TGF/SMADs signaling activity, antioxidant, anti-inflammatory, and apoptosis inducer.
Co-Authors Afifah Bambang Sutjiatmo, Afifah Bambang Azis, Rizal Fanny Rahardja Girsang, Ermi Hadiprasetyo, Dhanar Septyawan Hanna Sari Widya Kusuma, Hanna Sari Widya Harjanti, Mathelda Weni Marisca Evalina Gondokesumo, Marisca Evalina Narvikasari, Suci Ningrum, Siti Ratu Rahayu Novilla, Arina RAHMAT, DENI Sabrina, Adilah Hafizha Nur Sarwono, Sylvie Saufa, Zahra Qisthi Sijani Prahastuti Solihat, Ananda Khairunisa Takasenserang, Oktaviana Teresa Liliana Wargasetia Wahyu Surakusumah WAHYU WIDOWATI