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All Journal HAYATI Journal of Biosciences Science and Technology Indonesia
Hadiprasetyo, Dhanar Septyawan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Environmental Science Materials Science & Nanotechnology Physics

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Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells Gondokesumo, Marisca Evalina; Novilla, Arina; Prahastuti, Sijani; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Hadiprasetyo, Dhanar Septyawan; Surakusumah, Wahyu
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.228-237

Abstract

Leukemia, characterized by abnormal leukocyte proliferation, ranks ninth in Indonesia as the most common cancer. While treatments such as chemotherapy and radiation effectively target cancer cells, they also risk damaging healthy blood cells. This has spurred interest in exploring low-toxicity herbal compounds as potential therapies, with mangosteen peel emerging as a widely researched option. Nanotechnology, which has the potential to enhance the bioavailability of herbal compounds, is also a focus of extensive research. This study objective was to assess the impact of Mangosteen Peel Nanocrystal (MPN) on HL-60 leukemia cells by analyzing various parameters, including cytotoxicity, reactive oxygen species (ROS) levels, senescence, and gene expression changes. MPN was prepared with high-speed milling and characterized using particle size analyzers, microscopy, and stability assessments. HL-60 cells were cultured and subjected to MPN treatment. Cytotoxicity was evaluated using WST-8 assays, ROS levels were assessed using flow cytometry, and senescence analyses using Senescence-Associated b-Galactosidase Staining. AKT and FLT-1 gene expression were determined via qRT-PCR. MPN has been successfully characterized as a nanoparticle based on size, stability, and morphology. MPN has an impact on leukemia cells by increasing cytotoxicity, decreasing ROS levels, inducing senescence, and modulating AKT and FLT-1 gene expressions. The findings suggest potential implications for MPN in targeting leukemia cells. The study sheds light on the promising effects of MPN in leukemia cell models, indicating its potential applications in targeting cancer cells, inducing senescence, decreasing ROS levels, and modulating gene expressions related to cell survival and proliferation.
The Antiaging Potential of Serum Formulations from Centella asiatica, Curcuma longa, Aloe vera, Rosa centifolia, and Salmon DNA on Injured Human Fibroblast Cells Girsang, Ermi; Wargasetia, Teresa Liliana; Rahmat, Deni; Gondokesumo, Marisca Evalina; Harjanti, Mathelda Weni; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Saufa, Zahra Qisthi; Takasenserang, Oktaviana; Hadiprasetyo, Dhanar Septyawan
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.623-631

Abstract

External aging factors such as UV exposure, pollution, and lifestyle choices contribute to skin aging, resulting in deep wrinkles, fine lines, and rough skin, which can lower self-confidence. Plant extracts have been widely studied for their antiaging potential, while Salmon DNA has shown promise in stimulating collagen production. This study explores the formulation of a serum combining Centella asiatica, Curcuma longa, Aloe vera, Rosa centifolia, and Salmon DNA for its antiaging effects on injured human fibroblast cells. The serum was formulated using extracts from C. asiatica, C. longa, A. vera, R. centifolia, and Salmon DNA. Antioxidant activity was evaluated with the DPPH method, cytotoxicity using the WST-8 assay, and gene expression through qRT-PCR for COL1A1, TGF-β1, HYAL-1, and FGF-2. The serum exhibited weak antioxidant activity (IC50 = 373.33 µg/ml) and reduced cell viability at high concentrations. Gene expression analysis revealed increased expression of COL1A1, TGF-β1, and FGF-2, along with reduced HYAL-1 expression in injured BJ cells. The formulated serum shows potential as an antiaging agent, promoting collagen production and reducing hyaluronidase activity.
Potential of Bitter Melon (Momordica charantia L.) Extract for Chronic Kidney Disease Based on In Vitro Study via TGF/SMADs Signaling, Antioxidant, Antiinflammation, Apoptosis Inducer Activities Prahastuti, Sijani; Rahardja, Fanny; Wargasetia, Teresa Liliana; Zahiroh, Fadhilah Haifa; Sabrina, Adilah Hafizha Nur; Kusuma, Hanna Sari Widya; Azis, Rizal; Hadiprasetyo, Dhanar Septyawan; Ningrum, Siti Ratu Rahayu; Widowati, Wahyu; Sarwono, Sylvie
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.538-551

Abstract

Chronic kidney disease (CKD) is a physiological abnormality in the kidneys whose prevalence is expected to continue to increase. On the other hand, Bitter melon (Momordica charantia L.) is known to have the potential to manage CKD. This study explores the compound content of M. charantia ethanol extract (MCEE) and its potential for CKD based on in vitro assays. To model chronic kidney disease (CKD), SV40 MES-13 (mouse glomerular mesangial) cells were exposed for 3 days to 20 mM glucose. After glucose induction, the cells were subjected with different concentrations of MCEE (Momordica charantia L. ethanolic extract). The chemical profile of MCEE was analyzed using LC/MS-MS. Cell viability was examined through the WST assay, while intracellular ROS and apoptosis levels were measured by flowcytometry. Colorimetry was used to analyze SOD, MDA, and CAT levels. ELISA was used to analyze inflammatory proteins (TGF-β 1, IL-6, TNF-α, IL-1β ) levels. Meanwhile, the relative gene expression of SMAD-2, SMAD-3, SMAD-4, SMAD-7 was examined through qRT-PCR. The results exhibited that MCEE contains cucurbitane p-coumaric, ferulic acid, caffeic acid, gallic acid, chlorogenic acid, and epicatechin. MCEE was also known to be non-toxic to SV40 MES-13 cells. In addition, MCEE reduced intracellular ROS levels, MDA, necrosis levels, and inflammatory proteins, while also regulating SMAD-2, SMAD-3, and SMAD-4 gene expression. MCEE increased levels of CAT, and SOD, and regulated SMAD-7 gene expression in the CKD cells model. The most effective MCEE is MCEE 50 μg/mL. MCEE demonstrated potential as a CKD treatment based on in vitro studies through TGF/SMADs signaling activity, antioxidant, anti-inflammatory, and apoptosis inducer.
The Potential of Ethanol Extract of Pasak Bumi Roots (Eurycoma longifolia Jack) as an Anti-Prostate Cancer In Vitro Against PC-3 Cells Kania, Nia; Rahman, Eka Yudha; Priyandoko, Didik; Sabrina, Adilah Hafizha Nur; Widowati, Wahyu; Azis, Rizal; Annaba, Aziz; Hadiprasetyo, Dhanar Septyawan; Alexandro, Garry
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.452-466

Abstract

The prevalence of prostate cancer cases in men is expected to continue increase. In 2040, it is estimated that there will be 2.293.818 new cases and a 1.05% increase in the death rate due to prostate cancer. Eurycoma longifolia Jack roots extract (ELE) has potential as an alternative treatment. This study aims to analyze ELE potential as an anti-prostate cancer agent through in silico assay and in vitro assays on the prostate cancer cell line (PC-3). ELE compounds were docked against Casp-3, Casp-8, HAX-1, p27, and PTEN. In vitro assays on PC-3 cells were used, namely cell viability (WST-8), ROS levels; cell cycle; and cell apoptosis (flow cytometry), PC-3 cell senescence (μ-Galactosidase staining), Casp-3; Casp-8; HAX-1; p27; and PTEN gene expression (qRT-PCR). All proteins target were successfully docked with ELE compounds and presented binding interactions. ELE is known to reduce viability, intracellular ROS levels, live cells, necrosis, and reduce HAX-1 gene expression, and inhibit the cell cycle G0/G1 phase. ELE can also increase inhibition, senescence, late and early apoptosis, and Casp-3, Casp-8, p27, and PTEN gene expression. ELE 100 μg/mL is the most effective concentration. ELE has potential as an anti-prostate cancer agent through apoptosis, cell cycle, and antioxidant pathways
Antimicrobial and Antioxidant Activities of Black Cumin Seed (Nigella sativa) Ethanol Extract Reni Yuslianti, Euis; Susanto, Agus; Sutjiatmo, Afifah Bambang; Widowati, Wahyu; Ayuni, Vini; Hadiprasetyo, Dhanar Septyawan
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1273-1282

Abstract

Oral health faces significant challenges due to increasing dental infections by pathogenic microbes such as Porphyromonas gingivalis, Streptococcus mutans, and Candida albicans. Topical antioxidants in toothpaste, gel, and mouthwash are often used to treat dental diseases. There is a growing interest in finding natural alternatives for oral hygiene without side effects compared to conventional toothpaste formulations. Nigella sativa (black cumin) has various pharmaceutical efficacy, making it a valuable plant-based source medicinal compound. This study evaluated black cumin ethanol extract's (BCSE) antimicrobial and antioxidant activities. Antibacterial effectiveness was evaluated through Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and disc diffusion assays against P. gingivalis, S. mutans, and C. albicans. Antioxidant activity was reported based on DPPH scavenging, ABTS reduction, H2O2 scavenging, and NO scavenging assays. Black cumin seed ethanolic extract (BCSE) showed antibacterial activity of 4.49 mm, 4.33 mm, and 7.12 mm for P. gingivalis, S. mutans, and C. albicans, respectively, with zones of inhibition increasing in a concentration-dependent manner (p<0.05). MIC and MBC evaluations also revealed that MIC for S. mutans was achieved at 12.5% BCSE concentration, while P. gingivalis and C. albicans required 25%, and MBC reached 100%. The IC50 for DPPH, ABTS, NO, and H2O2 were 25.41, 69.93, 87.18, and 95.16 μg/ml, respectively. The antioxidant activity increased in a concentration-dependent manner, especially at 100 µg/ml of BCSE. Based on these results, BCSE could be an optional ingredient in toothpaste.
Co-Authors Afifah Bambang Sutjiatmo, Afifah Bambang Agus Susanto Alexandro, Garry Annaba, Aziz Ayuni, Vini Azis, Rizal Didik Priyandoko Eka Yudha Rahman Euis Reni Yuslianti, Euis Fanny Rahardja Girsang, Ermi Hanna Sari Widya Kusuma, Hanna Sari Widya Harjanti, Mathelda Weni Marisca Evalina Gondokesumo, Marisca Evalina Nia Kania Ningrum, Siti Ratu Rahayu Novilla, Arina RAHMAT, DENI Sabrina, Adilah Hafizha Nur Sarwono, Sylvie Saufa, Zahra Qisthi Sijani Prahastuti Takasenserang, Oktaviana Teresa Liliana Wargasetia Wahyu Surakusumah WAHYU WIDOWATI Zahiroh, Fadhilah Haifa