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Potensi Asam Lemak Pada Minyak Kelapa Murni Dalam Menghambat Candida Albicans Secara In Vitro Novilla, Arina; Nursidika, Perdina; Resmelia, Meyli
Majalah Kedokteran Bandung Vol 48, No 4 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Kandidiasis merupakan penyakit infeksi Candida baik primer maupun sekunder. Penyebab utama kandidiasis adalah Candida albicans (C. albicans). Pengobatan kandidiasis dilakukan dengan pemberian obat anti jamur, terutama nistatin, amfoterisin-B dan azole, tetapi toksisitas dan resistensi obat ini menjadi masalah potensial. Diperlukan pemakaian obat lain yang lebih aman. Salah satunya adalah pemanfaatan bahan alami yaitu minyak kelapa murni. Tujuan penelitian ini adalah untuk mengetahui potensi asam lemak minyak kelapa murni dalam menghambat pertumbuhan C. albicans. Desain penelitian eksperimental. Penelitian menggunakan difusi Kirby Bauer untuk mendapatkan konsentrasi hambat minimum minyak kelapa murni dalam menghambat C. albicans. Penelitian dilakukan pada bulan Septemberâ€“Oktober 2010 di Laboratorium Kimia dan Mikrobiologi Sekolah Tinggi Ilmu Kesehatan Jenderal Achmad Yani Cimahi. Hasil menunjukkan asam lemak dalam fraksi n-heksan dan metanol hasil hidrolisis berpotensi dalam menghambat pertumbuhan C. albicans. Fraksi n-heksan konsentrasi minimal yang memberikan hambatan yaitu 75% dengan diameter hambatan rata-rata sebesar 3,3 mm, sebanding dengan antibiotik nistatin 195 unit. Fraksi metanol memberikan hambatan dimulai pada konsentrasi 75% dengan diameter hambatan rata-rata sebesar 4 mm, sebanding dengan antibiotik nistatin 195 unit. Hasil konsentrasi hambat minimum masing-masing asam lemak untuk fraksi metanol konsentrasi 25% dan fraksi n-heksan 100%. Simpulan, asam lemak pada minyak kelapa murni dapat menghambat C. albicans. [MKB. 2016;48(4):200â€“4]Kata kunci: asam lemak, Candida albicans, in vitro, minyak kelapa murniPotential Of Virgin Coconut Oil Fatty Acid to Inhibit the Growth of Candida Albicans In VitroAbstractCandidiasis is Candida infection both primary or secondary diseases. The main cause of candidiasis is Candida albicans (C. albicans). Candidiasis is treated by antifungal drug treatment, especially nistatin, amphotericin-B, and azole, but toxicity and drug resistance become potential problems. Therefore, other method is needed as safer treatment. One of natural resources which has antifungal is virgin coconut oil. Research design was experimental. The objective of study was determine the potential of fatty acid in virgin coconut oil to inhibit C. albicans. This study used Kirby Bauer Method to determine minimum inhibition concentration. The study was performed in September-October 2010 at Chemistry and Microbiology Laboratory School of Health Sciences Jenderal Achmad Yani Cimahi. The result showed the fatty acid of n-hexane and methanol fractions after hydrolysed were potential to inhibit the growth of C. albicans. Minimum inhibitory concentration against C. albicans was 75% with average diameter of inhibiting zone 3.3 mm was equal to nistatin 195 units. The methanol fraction inhibited C. albicans start from 75% with average diameter 4 mm or equal to nistatin 195 units. Minimum fungicidal concentration showed C. albicans inhibited by methanol fraction 25% and n-hexane fraction with concentration 100%. In conclusion, fatty acid of virgin coconut oil was able to inhibit C. albicans. [MKB. 2016;48(4):200â€“4]Key words: Candida albicans, fatty acids, in vitro, virgin coconut oil

HUBUNGAN PENGETAHUAN DENGAN PERILAKU PENCEGAHAN HIV/AIDS PADA MAHASISWA TINGKAT IV PROGRAM STUDI X FAKULTAS X UNIVERSITAS X TAHUN 2023 Santoso S. Kep., Ners M. Kep, Mochamad Budi; Novilla, Arina; Mulyono, Ilham; Juhaeriah, Juju; Kamdah, Musri
JURNAL KESEHATAN KARTIKA Vol. 19 No. 1 (2024): Jurnal Kesehatan Kartika
Publisher : Faculty of Health Science and Technology, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkkes.v19i1.292

T Background: The high rate of HIV/AIDS cases in late adulthood needs to be anticipated with prevention at an earlier age, namely in late adolescence to reduce the incidence of HIV/AIDS in late adulthood and late adolescence. Objective: This research was conducted with the aim of knowing the relationship between knowledge and HIV/AIDS prevention behavior in students. Method: The Cross-sectional analytic survey design used a total sampling technique with a total sample of 146 respondents. Collecting data using a questionnaire with primary data taken directly from the respondents. Univariate and bivariate analyses were performed using the Chi-square test. Results: The results showed that 102 out of 122 (83.6%) respondents had good knowledge and good behavior. While 11 out of 24 (45.8%) respondents had less knowledge and less behavior. The results of the Chi-Square test showed that there was a relationship between knowledge and HIV/AIDS prevention behavior with a P value of 0.003 <0.05. ConclusionThe conclusion of this study is that there is a relationship between Knowledge and HIV/AIDS Prevention Behavior in students. Suggestions for institutions, It is hoped that they can make routine policies/programs regarding fostering sex education, oblige students to take part in student activities, and conduct screening for HIV/AIDS

WEBINAR PEMULASARAN JENAZAH COVID-19 DI LUAR FASILITAS PELAYANAN KESEHATAN Wasdili, Fini Ainun Qolbi; Mahargyani, Wikan -; Romlah, Sitti; Herawati, Iis; Novilla, Arina; Furqon, Ayi; Ningrum, Nining Ratna
DHARMAKARYA: Jurnal Aplikasi Ipteks untuk Masyarakat Vol 13, No 1 (2024): Maret, 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/dharmakarya.v13i1.40607

Pandemi Covid 19 di Indonesia semakin meningkat, tenaga kesehatan, masyarakat dan pengurus Dewan Kemakmuran Mesjid (DKM) berperan dalam membantu pemulasaran jenazah. Prodi Teknologi Laboratorium Medis D-3 memfasilitasi pelaksanaan webinar pemulasaran jenazah Covid-19 untuk wilayah cimahi dan Kabupaten Bandung. Tahapan pelaksanaan terdiri dari identifikasi permasalahan pandemi Covid-19, menyusun dan mengkordinasikan kegiatan webinar, penyebaran informasi pendaftaran dan kegiatan, pelaksanaan kegiatan webinar, sesi diskusi, dan evaluasi. Tingkat pengetahuan masyarakat terhadap pemulasaran jenazah Covid-19 sebanyak 43% tidak mengetahui. Berdasarkan hasil kuesioner 96% menyatakan sangat bermanfaat dan penilaian keseluruhan kegiatan adalah 75% sangat sesuai. Di akhir acara perwakilan DKM diberikan Gown untuk membantu proses pemulasaran jenazah Covid-19.

The Effect Of Giving Green Tea (Camellia Sinensis L.) on Leukocyte Counts of Smokers Novilla, Arina; Wasdili, Fini Ainun Qolbi; Suseno, Novalia Rachma
Jurnal Kesehatan Pasak Bumi Kalimantan Vol 7, No 1 (2024): JKPBK Juni 2024
Publisher : Fakultas Kedokteran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/j.kes.pasmi.kal.v7i1.12309

Background: Cigarette smoke consists of the main smoke (main stream) which contains 25% levels of hazardous materials and side streams which contain 75% levels of hazardous materials. Cigarette smoke contains more than 4000 harmful chemicals, including nicotine, tar, lead, carbon monoxide (CO) and various other free radicals which are carcinogenic. Each puff of cigarette smoke contains 1015 oxidative free radicals which are the main source of oxidative stress which has the potential as a mediator of endothelial dysfunction, increased blood thrombogeneity, and inflammatory response. Someone who smokes continuously for a long time is known to have a leukocyte count 20-25% higher than people who don't smoke. It is known that the entry of nicotine into the body causes leukocytosis due to the circulation of catecholamines due to the increase in hormones such as epinephrine and cortisol by nicotine. Objective: Seeing the leukocyte count values in smokers who consume green tea (Camellia sinensis L). Methods : This research method uses descriptive method. The research samples were taken from active smokers in the Unjani FITKES environment as many as 28 samples. Data collection was carried out by filling out questionnaires and consent forms. Then an examination of the leukocyte count was carried out including the value of eosinophils, segment neutrophils, stem neutrophils, lymphocytes and monocytes. Data analysis is reported as a percentage. Results : Based on the results of the study, the sig value for eosinophils was (0.191), segment neutrophils (0.876), stem neutrophils (0.080), lymphocytes (0.333) and monocytes were smaller (0.003). it can be concluded that green tea has an effect but not significant on the leukocyte count value to neutralize free radicals caused by smoking. Conclusion : Based on this study, it was concluded that from 28 samples of active smokers, it was found that 17 people (60.7%) had an increase in monocytes. It can be concluded that green tea has an effect, but not significantly, on the leukocyte count value to neutralize free radicals caused by smoking.

Skrining Talasemia Pada Mahasiswa TLM (D3) Fakultas Ilmu Dan Teknologi Kesehatan Universitas Jenderal Achmad Yani Cimahi: Skrining Talasemia Pada Mahasiswa TLM (D3) Fakultas Ilmu Dan Teknologi Kesehatan Universitas Jenderal Achmad Yani Cimahi Novilla, Arina; Clarisa, ⁠Tiara Nur; Furqon, Ayi; Romlah, Sitti
Jurnal Ilmiah Kesehatan Vol 16 No 2 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v16i2.2327

Menurut data Organisasi Kesehatan Dunia (WHO) pada tahun 2021, diperkirakan bahwa sekitar 20% dari seluruh populasi dunia menderita talasemia beta mayor. Di Indonesia, prevalensi talasemia berkisar antara 6-10%, dan provinsi Jawa Barat mempunyai kasus talasemia mayor tertinggi. Talasemia mayor terjadi akibat perkawinan antara individu yang membawa gen talasemia atau menderita talasemia minor, yang seringkali tanpa gejala, sehingga deteksi dini sangat penting untuk mencegah kelahiran anak dengan talasemia mayor.Penelitian ini bertujuan untuk mengetahui skrining talasemia menggunakan uji fragilitas osmotic (OTOF/One Tube Osmotic Fragility), hasil positif dilanjutkan dengan penentuan nilai indeks eritrosit, lalu dilanjutkan dengan penentuan Indeks Mentzer. Penelitian ini menggunakan metode deskriptif, subjek penelitian adalah mahasiswa TLM (D3) di Fakultas Ilmu dan Teknologi Kesehatan Universitas Jenderal Achmad Yani, Cimahi. Berdasarkan hasil penelitian dari 155 mahasiswa TLM (D3), hasil skrining talasemia minor menunjukkan bahwa 10 orang (6%) menunjukkan hasil OTOF positif. Hasil yang positif dilanjutkan pemeriksaan darah lengkap untuk menentukan Indeks Eritrosit, sebanyak 8 orang (80%) memiliki MCV <80 fL dan MCH <27 pg. Hasil MCV dan MCH rendah tersebut kemudian ditentukan nilai Indeks Mentzer dan didapatkan sebanyak 2 orang (25%) memiliki Indeks Mentzer <13. Dari penelitian ini disimpulkan bahwa terdapat 2 orang (1%) yang terindikasi menderita talasemia beta minor, dan disarankan selanjutnya untuk dilakukan pemeriksaan elektroforesis hemoglobin.

Perbandingan Kadar Hemoglobin Menggunakan POCT (Point Care Of Testing) dengan Alat Hematology Analyzer Pada Pasien Normal dan Anemia Fauzi, Anggi; Novilla, Arina; Ningrum, Nining Ratna; Herawati, Iis
Jurnal Ilmiah Kesehatan Vol 16 No 2 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v16i2.2329

Di laboratorium pemeriksaan hemoglobin merupakan salah satu pemeriksaan skrining, penggunaan alat bervariasi salah satunya adalah menggunakan POCT. Alasan penggunaan alat ini karena lebih efisien dibanding hematology analyzer, seperti pengerjaannya lebih mudah karena POCT biasanya bersifat portabel, sehingga bisa digunakan di berbagai lokasi tanpa perlu membawa sampel ke laboratorium pusat sehingga POCT bisa dijadikan alternatif pada kondisi tertentu. Alat POCT biasanya lebih murah untuk dibeli awalnya karena ukurannya yang kecil dan teknologi yang lebih sederhana. Tujuan penelitian ini membandingkan kadar hemoglobin yang diukur memakai alat Point of Care Testing (POCT) dan Hematology Analyzer pada pasien dengan kondisi normal, anemia sedang, dan anemia berat. Metode yang digunakan adalah penelitian komparatif dengan desain cross sectional. Besaran sampel digunakan dalam penelitian ini adalah 40 pasien dibagi secara proporsional menjadi 20 pasien dengan kadar hemoglobin normal, 10 pasien dengan anemia sedang dan 10 pasien dengan anemia berat. Hasil penelitian rerata kadar hemoglobin kategori normal pada POCT adalah 14,37 mg/dL, sedangkan pada hematology analyzer adalah 13,95 mg/dL. Rerata kadar hemoglobin kategori anemia sedang pada POCT adalah 9,45 mg/dL, sedangkan pada hematology analyzer adalah 8,77 mg/dL. Rerata kadar hemoglobin kategori anemia berat pada POCT adalah 6,42 mg/dL, sedangkan pada hematology analyzer adalah 5,82 mg/dL. Hasil penelitian menyimpulkan bahwa hasil uji Independent t-test ketiga kategori didapat nilai p value >0,05 menunjukkan tidak ada perbedaan signifikan pengukuran kadar hemoglobin menggunakan POCT dengan hematology analyzer. Kata Kunci: Point of Care Testing, Hematology Analyzer, Anemia

Skrining Talasemia Minor Pada Pendonor di Unit Donor Darah Palang Merah Indonesia Kabupaten Majalengka Winengsih, Wiwin; Novilla, Arina; Ningrum, Nining Ratna; Khairinisa, Gina
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2330

Thalassemia is a hereditary hemolytic disease caused by a disorder in the synthesis of hemoglobin within red blood cells. Thalassemia prevention programs are beneficial for reducing or breaking the chain of thalassemia by preventing marriages between thalassemia carriers, which could result in thalassemia sufferers. One preventive measure is thalassemia screening. This study aims to identify the presence of thalassemia minor carriers among donors at the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. The study is a descriptive quantitative research with a sample size of 92 donors who visited the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. The thalassemia screening included hemoglobin tests, osmotic fragility tests, and erythrocyte indices. The results showed that all 92 donors had normal hemoglobin levels with an osmotic fragility test showing negative results using OTOF method. Therefore, further examination of the erythrocyte index was not conducted.. Based on these results, it can be concluded that there were no thalassemia minor carriers among the donors at the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. Keywords :Thalassemia, Donor,Osmotic Fragility, Hemoglobin

Anticancer Effectivity of Nanocrystals Derived from Mangosteen (Garcinia mangostana) Peel Extract on Leukemia HL-60 Cells Gondokesumo, Marisca Evalina; Novilla, Arina; Prahastuti, Sijani; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Hadiprasetyo, Dhanar Septyawan; Surakusumah, Wahyu
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.228-237

Leukemia, characterized by abnormal leukocyte proliferation, ranks ninth in Indonesia as the most common cancer. While treatments such as chemotherapy and radiation effectively target cancer cells, they also risk damaging healthy blood cells. This has spurred interest in exploring low-toxicity herbal compounds as potential therapies, with mangosteen peel emerging as a widely researched option. Nanotechnology, which has the potential to enhance the bioavailability of herbal compounds, is also a focus of extensive research. This study objective was to assess the impact of Mangosteen Peel Nanocrystal (MPN) on HL-60 leukemia cells by analyzing various parameters, including cytotoxicity, reactive oxygen species (ROS) levels, senescence, and gene expression changes. MPN was prepared with high-speed milling and characterized using particle size analyzers, microscopy, and stability assessments. HL-60 cells were cultured and subjected to MPN treatment. Cytotoxicity was evaluated using WST-8 assays, ROS levels were assessed using flow cytometry, and senescence analyses using Senescence-Associated b-Galactosidase Staining. AKT and FLT-1 gene expression were determined via qRT-PCR. MPN has been successfully characterized as a nanoparticle based on size, stability, and morphology. MPN has an impact on leukemia cells by increasing cytotoxicity, decreasing ROS levels, inducing senescence, and modulating AKT and FLT-1 gene expressions. The findings suggest potential implications for MPN in targeting leukemia cells. The study sheds light on the promising effects of MPN in leukemia cell models, indicating its potential applications in targeting cancer cells, inducing senescence, decreasing ROS levels, and modulating gene expressions related to cell survival and proliferation.

Skrining Talasemia Minor Pada Pendonor di Unit Donor Darah Palang Merah Indonesia Kabupaten Majalengka Winengsih, Wiwin; Novilla, Arina; Ningrum, Nining Ratna; Khairinisa, Gina
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2330

Thalassemia is a hereditary hemolytic disease caused by a disorder in the synthesis of hemoglobin within red blood cells. Thalassemia prevention programs are beneficial for reducing or breaking the chain of thalassemia by preventing marriages between thalassemia carriers, which could result in thalassemia sufferers. One preventive measure is thalassemia screening. This study aims to identify the presence of thalassemia minor carriers among donors at the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. The study is a descriptive quantitative research with a sample size of 92 donors who visited the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. The thalassemia screening included hemoglobin tests, osmotic fragility tests, and erythrocyte indices. The results showed that all 92 donors had normal hemoglobin levels with an osmotic fragility test showing negative results using OTOF method. Therefore, further examination of the erythrocyte index was not conducted.. Based on these results, it can be concluded that there were no thalassemia minor carriers among the donors at the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. Keywords :Thalassemia, Donor,Osmotic Fragility, Hemoglobin

Skrining Talasemia Pada Mahasiswa TLM (D3) Fakultas Ilmu Dan Teknologi Kesehatan Universitas Jenderal Achmad Yani Cimahi: Skrining Talasemia Pada Mahasiswa TLM (D3) Fakultas Ilmu Dan Teknologi Kesehatan Universitas Jenderal Achmad Yani Cimahi Novilla, Arina; Clarisa, ⁠Tiara Nur; Furqon, Ayi; Romlah, Sitti
Jurnal Ilmiah Kesehatan Vol 16 No 2 (2024): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v16i2.2327

Menurut data Organisasi Kesehatan Dunia (WHO) pada tahun 2021, diperkirakan bahwa sekitar 20% dari seluruh populasi dunia menderita talasemia beta mayor. Di Indonesia, prevalensi talasemia berkisar antara 6-10%, dan provinsi Jawa Barat mempunyai kasus talasemia mayor tertinggi. Talasemia mayor terjadi akibat perkawinan antara individu yang membawa gen talasemia atau menderita talasemia minor, yang seringkali tanpa gejala, sehingga deteksi dini sangat penting untuk mencegah kelahiran anak dengan talasemia mayor.Penelitian ini bertujuan untuk mengetahui skrining talasemia menggunakan uji fragilitas osmotic (OTOF/One Tube Osmotic Fragility), hasil positif dilanjutkan dengan penentuan nilai indeks eritrosit, lalu dilanjutkan dengan penentuan Indeks Mentzer. Penelitian ini menggunakan metode deskriptif, subjek penelitian adalah mahasiswa TLM (D3) di Fakultas Ilmu dan Teknologi Kesehatan Universitas Jenderal Achmad Yani, Cimahi. Berdasarkan hasil penelitian dari 155 mahasiswa TLM (D3), hasil skrining talasemia minor menunjukkan bahwa 10 orang (6%) menunjukkan hasil OTOF positif. Hasil yang positif dilanjutkan pemeriksaan darah lengkap untuk menentukan Indeks Eritrosit, sebanyak 8 orang (80%) memiliki MCV <80 fL dan MCH <27 pg. Hasil MCV dan MCH rendah tersebut kemudian ditentukan nilai Indeks Mentzer dan didapatkan sebanyak 2 orang (25%) memiliki Indeks Mentzer <13. Dari penelitian ini disimpulkan bahwa terdapat 2 orang (1%) yang terindikasi menderita talasemia beta minor, dan disarankan selanjutnya untuk dilakukan pemeriksaan elektroforesis hemoglobin.

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