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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana SIMBIOSIS Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) The Indonesian Biomedical Journal Sains Natural: Journal of Biology and Chemistry Jurnal Widya Biologi Tropical Plantation Journal Forest and Nature

I GUSTI AYU MANIK ERMAYANTI

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 260506

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Efektivitas Ekstrak Hipofisa Ayam Broiler (Gallus sp.) Terhadap Kinerja Reproduksi Dan Kelulushidupan Larva Ikan Lele Sangkuriang (Clarias sp.) Adevalentin Lesik; Iriani setyawati; NI Gusti Ayu Manik Ermayanti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p05

The pituitary gland of broiler chickens (Gallus sp.) is empirically known to have gonadotropins to accelerate ovulation in fish. This research was conducted to determine the reproductive performance and survival of sangkuriang catfish (Clarias sp.) Larvae of adult male and female mature gonads by 20 pairs. This study used a Completely Randomized Design consisting of 4 treatments and 5 replications to obtain 20 experimental units. Doses of pituitary extract used is without injection of pituitary chicken pituitary extract (P0), injection of pituitary chicken pituitary extract with a dose of 600 mg/kgbb (P1), 900 mg/kgbb (P2) and 1200 mg/kgbb sangkuriang catfish (P3). Intramuscular injection of extracts in the ventral dorsal fin with a volume of 0.5 mL/kgbb. Descriptive data analysis for gonad maturity level, ovulation response, egg performance. Statistical analysis of data for morphometric aspects using the Wilcoxon test, for latency time, relative fecundity, fertilization success, hatching success, and larval survival aspects using One Way Anova using SPSS version 25. The results of statistical analysis showed significant differences in reproductive performance (relative latency and fecundity time), successful fertilization and successful hatching of eggs, but there was no significant difference in survival (number of live larvae, length and weight) larvae of sangkuriang catfish (Clarias sp.). Broiler chicken pituitary extract (Gallus sp.) can improve reproductive performance, fertilization and hatching success but does not affect egg performance and survival of sangkuriang catfish (Clarias sp.) larvae. Keyword: catfish, pituitary extract, reproduction, survival

Gambaran Histologi Lambung Dan Duodenum Mencit (Mus musculus L.) Jantan Yang Diberi Ekstrak Daun Kersen (Muntingia calabura L.) Setelah Diinduksi Monosodium Glutamat (MSG) Ni Wayan Adya Puttry Purna Yogini; Ngurah Intan Wiratmini; Ni Gusti Ayu Manik Ermayanti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p02

The antioxidant contained in kersen (Muntingia calabura L.) leaves has been known to be able to prevent animal tissue damages. Monosodium glutamate (MSG) is a food additive substance associated with histological damage of duodenum as well as increases secretion of HCl in stomach if consumed in excess amount. This study aimed to investigate the histological features of stomach and duodenum of monosodium glutamate-induced mice (M. musculus) after treated with kersen leaves extract, as well as establishing its optimum dose. This study used a Completely Randomized Design, with 25 male mice assigned to 5 treatment groups: 2 control groups i.e. negative control (K-) given aquades and positive control (K+) given 0.7mg/body weight MSG; and 3 intervention groups which were given kersen leaves extract as much as 0.075 mg/g bw (P1), 0.15 mg/g bw (P2) and 0.3 mg/g bw (P3). Both kersen leaves extract and MSG was administered orally once daily for 28 days. The parameters observed were histological features of stomach features (erosion of villi and inflammatory cells infiltration) and duodenum (erosion of villi, necrosis, and inflammatory cell infiltration). The results showed that all parameters observed in the aforementioned organs were significantly different (P<0.05) in intervention groups (P1, P2, dan P3) compared with positive control (K+). It is concluded that administration of kersen leaves extract improved the damages of stomach, duodenum, and liver of MSG-induced mice, and was best achieved at the dose of 0.3 mg/g bw.

PROFIL LIPID KELINCI (Lepus sp.) JANTAN LOKAL YANG DIBERI PAKAN KOMERSIAL DISUPLEMENTASI MINYAK HATI IKAN KOD NI GUSTI AYU MANIK ERMAYANTI; I GUSTI LANANG OKA; I GEDE MAHARDIKA; PUTU SUYADNYA
Jurnal Biologi Udayana Vol 22 No 1 (2018): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

ABSTRACT Cod liver oil as a source PUFA (Polyunsaturated Fatty Acid) has very diverse and complex benefits, including for heart health. This study aimed to improve the lipid profile of male local rabbit which was fed commercial supplementation of cod liver oil. The experimental design used was Completely Randomized Design (RAL) with four feed treatments, ie commercial feed without supplementation of cod liver oil (P0) as control, commercial feed supplemented by cod liver oil 1.5% (P1), commercial feed supplemented by oil liver of 3% cod (P2) and commercial feed supplementation of cod liver oil 4.5% (P3).Each treatment consists of eight rabbits. The variables observed were total cholesterol, triglyceride, LDL, and HDL levels. The data obtained were analyzed with ANOVA and if significantly different were tested further with DMRT. The results showed that supplementation of cod liver oil with different levels in commercial feed showed significantly different results (P <0.05) on total cholesterol, triglyceride, LDL, and HDL levels of local rabbit. It can be concluded that supplementation of cod liver oil in commercial feed to the extent 4.5% capable repair the lipid profile of male local rabbit because it can decreased total cholesterol levels, decrease triglycerides levels, decreased LDL levels, and increased HDL levels.

GANGGUAN SPERMATOGENESIS SETELAH PEMBERIAN MONOSODIUM GLUTAMAT PADA MENCIT (MUS MUSCULUS L.) A.A.SG.A SUKMANINGSIH; I GUSTI AYU MANIK ERMAYANTI; NGURAH INTAN WIRATMINI; NI WAYAN SUDATRI
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.702 KB)

This study was conducted to investigate the effect of monosodium glutamate (MSG) on spermatogenesis of male experimental mice.using 32 twelve-week old mice. They were divided randomly into 4 groups, each group consisted of 8 mice. One group was used as control, three groups were used as treatment, which received 1.5 mg/g body weight/day, 3 mg/g body weight/day and 4.5 mg/g body weight/day of MSG solution in destilled water for 35 days. The result showed that MSG treatment led to disturbance on spermatogenesis by reduction in some of the parameter studied. There was a significant (p < 0.05) decrease in the number of pachytene spermatocyte and spermatid 15, where as the decrease in spermatogonia was insignificant (p>0.05).

Histomorfometrik duodenum kelinci jantan setelah diberi pakan komersial disuplementasi minyak hati ikan kod Ni Gusti Ayu Manik Ermayanti; Ni Wayan Sudatri; Ni Nyoman Wirasiti; I Gusti Ayu Manik Widhyastini
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p10

Fish oil is a source of long-chain polyunsaturated fatty acids (LCPUFA) that can be used as supplementary feed in commercial feed to increase male rabbit productivity. PUFAs function in various physiological processes, including in the digestive system.This study aimed to determine the histomorphometrics of male rabbits duodenum after fed commercial feed supplemented by cod liver oil. The experimental design used was a Completely Randomized Design (CRD) with four feed packages, namely commercial feed without cod liver oil (P0) as a control, commercial feed supplemented with cod liver oil 3% (P1), 4.5% (P2) and 6% (P3). Each consists of five male local rabbits as replicates and given starting female rabbits 1-3 months. The parameters observed were villi height, villi width, crypt depth, and tunica mucosa thickness of the duodenum in male rabbits. The data obtained were analyzed with Anova and if significantly different were further tested with DMRT. The results showed that cod liver oil supplementation in commercial feed was significantly different (p<0.05) to the histomorphometric parameters (villi height, villi width, crypt depth, and thickness of the tunica mucosa) of the duodenum in male rabbits. Can be concluted that supplementation of cod liver oil in commercial feed can increase the histomorphometrics of male local rabbits duodenum.

VIABILITAS SPERMATOZOA PETAURUS BREVICEPS PAPUANUS T. NI MADE RAI SUARNI; I GUSTI AYU MANIK ERMAYANTI
Jurnal Biologi Udayana Vol 13 No 2 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Experiment to determine the spermatozoa viability of Petaurus berviceps papuanus T. has been performed. Observation was done under microscope and trypan blue was used as stain. Viable spermatozoa were counted from a total of 100 spermatozoa. It was found that 4,75 % of the spermazoa could be maintained for 7.5 hours in the DMEM (Dulbecco's modified Eagle's medium) at 35oC.

KUALITAS SPERMATOZOA MENCIT (MUS MUSCULUS L.) SETELAH PERLAKUAN INFUS KAYU AMARGO (QUASSIA AMARA LINN.) DAN PEMULIHANNYA NI GUSTI AYU MANIK ERMAYANTI; NI MADE RAI SUARNI
Jurnal Biologi Udayana Vol 14 No 2 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.711 KB)

Some studies have indicated that different types of plants can impair male fertility. The research aims to determine the effect of amargo wood intravenous feeding on the quality of mice sperm and its reversibility. The research design used was Random Full Factorial Design. Thirty male mice were divided into two groups of observations, i.e. group I (35 days after infusion) and group II (14 days after administration of infusion discontinued). Furthermore, each group was further subdivided into five sub-dose treatment groups, namely 0 (control), 0 (placebo), 1000, 2000, and 4000 mg/kg. Infusion was administered orally, once daily, for 35 days with a volume of 1 ml. The quality of sperm observed includes motility, viability, and morphology. The data obtained were tested by Anova. The result showed that amargo wood intravenous treatment has significant effect (P

PENGARUH PEMBERIAN EKSTRAK DAUN EKOR NAGA (Rhaphidophora pinnata, Schott) TERHADAP PERKEMBANGAN UTERUS MENCIT (Mus musculus) BETINA YANG TELAH DIOVARIEKTOMI Maria Antonia Margaretha Ferandez; Ngurah Intan Wiratmini; Ni Gusti Ayu Manik Ermayanti
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.418 KB)

Tanaman ekor naga (Rhaphidophora pinnata, Schott.) mengandung zat aktif dari golongan senyawa alkaloid, flavonoid, saponin, tanin dan triterpenoid/steroid. Zat aktif yang terkandung dalam tanaman ekor naga bersifat fitoestrogen. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak daun ekor naga terhadap perkembangan uterus mencit (Mus musculus) betina yang telah diovariektomi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari empat kelompok perlakuan dengan masing-masing enam ulangan. PerlakuanP0 sebagai kontrol diberi NaCl 0,9% dan perlakuan P1, P2 dan P3 diberi ekstrak daun ekor naga dengan dosis 50, 100 dan 150 mg/KgBB. Ekstrak daun ekor naga diberikan setiap hari secara oral selama 14 hari sebanyak 0,2 ml dengan metode gavage. Variabel yang diamati adalah bobot uterus, diameter uterus dan ketebalan endometrium. Data yang diperoleh dianalisis dengan One Way Anova dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa pemberian ekstrak daun ekor naga (R. pinnata, Schott) menyebabkan peningkatan ketebalan endometrium sebesar 38,7% dan peningkatan diameter uterus sebesar 30,3% dibanding dengan kelompok kontrol. Dosis optimal ekstrak daun ekor naga (R. pinnata, Schott) yang berpengaruh terhadap perkembangan uterus mencit adalah 100 mg/KgBB.

Pengaruh Ekstrak Daun Kopi Robusta (Coffea canephora Pierre ex A. Froehner) terhadap Kualitas Spermatozoa Mencit (Mus musculus L.) Yang Terpapar Asap Rokok. I Komang Alit Saputra; Ni Gusti Ayu Manik Ermayanti; A.A. S. A. Sukmaningsih
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.619 KB)

This study aims to determine the effect of given robusta coffee (Coffea canephora Pierre ex A. Froehner) leaf extract on the spermatozoa quality of mice (Mus musculus L.) exposed to cigarette smoke. The experiment was using a completely randomized design (CRD) with five treatments and each consisted of six mice as replications. Each treatment was K- (without exposure to cigarette smoke), K+ (exposure to cigarette smoke + 0.5% Na-CMC), P1 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 60 mg/kgbw), P2 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 120 mg/kgbw) and P3 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 180 mg/kgbw). On the 36th day, mice were taken down by anesthesia and followed by surgery to collect spermatozoa. The parameters for this observation were the concentration, motility, viability, morphology, and integrity of the spermatozoa membrane. The data obtained were analyzed statistically with One Way Anova and further tested with DMRT. The results showed that the treatment of robusta coffee leaf exstract in mice exposed to cigarette smoke had a significant effect (P<0,05) on the quality of spermatozoa.

DIFFERENCE IN SPERM QUALITY IN PATIENTS WITH TYPE 1 AND 2 DIABETES MELLITUS IN RSUD. LAMADUKELLENG, SENGKANG, SULAWESI SELATAN Andi Ratu Bulqis; Ni Gusti Ayu Manik Ermayanti; Nyoman Wirasiti
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Diabetes mellitus merupakan gangguan metabolisme yang disebabkan kurangnya hormon insulin dan menyebabkan kelainan metabolisme karbohidrat, protein dan lemak. Diabetes mellitus akan mempengaruhi beberapa kinerja organ dalam tubuh manusia. Salah satunya yaitu berpengaruh terhadap produksi kualitas sperma pada tubulus seminiferus. Tujuan dilakukan penelitian ini adalah untuk mengetahui perbedaan kualitas sperma pada pasien penderita diabetes mellitus tipe 1 dan 2 di Rumah Sakit Umum Daerah Lamadukelleng, Sengkang. Sampel diambil dari 30 laki-laki yang bersedia sebagai probandus sesuai dengan kriteria yang telah ditentukan yaitu penderita DM tipe 1 dan 2, berumur 20-45 tahun. Kemudian dimasukkan kelompok kontrol, DM 1 dan DM 2 menggunakan metode Komperatif dengan jumlah probandus masing-masing kelompok sebanyak 10 orang. Metode analisa kualitas sperma yang dilakukan di Laboratorium Andrologi, Rumah Sakit Umum Daerah Lamadukelleng, Sengkang adalah makler chamber, kemudian dilakukan pemeriksaan analisa kualitas sperma seperti pada umumnya. Data yang didapat dari hasil penelitian ini berupa data kualitatif dan kuantitatif. Data kualitatif meliputi morfologi, disajikan dalam bentuk gambar. Kemudian data kuantitatif meliputi, penghitungan konsentrasi, motilitas dan morfologi disajikan dalam bentuk tabulasi data. Setelah itu data kuantitatif dibaca dalam metode penghitungan statistik menggunakan SPSS Versi 24, dengan One Way Anova untuk melihat perbedaan antara sampel DM tipe 1 dan 2 yang berbeda signifikan (p < 0,05). Kesimpulan penelitian ini yaitu terdapat perbedaan konsentrasi, motilitas dan morfologi pada pasien penderita DM tipe 1 dan 2.

Co-Authors A.A.S. A. Sukmaningsih Adevalentin Lesik Andi Ratu Bulqis Anom Suardika Budayasa, Anak Agung Gede Raka Desak Putu Sukraniti Endang Sri Widiyanti I Gede Mahardika I Gusti Ayu Manik Widhyastini I Gusti Ayu Sugi Wahyuni I GUSTI LANANG OKA I Gusti Lanang Oka I Komang Alit Saputra I Made Jawi I Putu Suyadnya, I Putu I Wayan Juniarsana Ida Bagus Made Suaskara Iriani Setyawati IRIANI SETYAWATI Iriani Setyawati Kusuma, Anak Agung Ngurah Jaya Maria Antonia Margaretha Ferandez Messalina Lovenia Salampessy, Messalina Lovenia Ngurah Intan Wiratmini Ni Luh Suriani Ni Made Rai Suarni Ni Nyoman Ayu Dewi Ni Nyoman Wirasiti Ni Wayan Adya Puttry Purna Yogini Ni Wayan Sudatri Putu Mila Ayustina PUTU SUYADNYA Silvana, Divika Sun, H. Sunny Tang, Yen-An Wibawa, I Gusti Ngurah Agung Satria

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