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Pengaruh Ekstrak Daun Kopi Robusta (Coffea canephora Pierre ex A. Froehner) terhadap Kualitas Spermatozoa Mencit (Mus musculus L.) Yang Terpapar Asap Rokok. I Komang Alit Saputra; Ni Gusti Ayu Manik Ermayanti; A.A. S. A. Sukmaningsih
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.619 KB)

Abstract

This study aims to determine the effect of given robusta coffee (Coffea canephora Pierre ex A. Froehner) leaf extract on the spermatozoa quality of mice (Mus musculus L.) exposed to cigarette smoke. The experiment was using a completely randomized design (CRD) with five treatments and each consisted of six mice as replications. Each treatment was K- (without exposure to cigarette smoke), K+ (exposure to cigarette smoke + 0.5% Na-CMC), P1 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 60 mg/kgbw), P2 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 120 mg/kgbw) and P3 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 180 mg/kgbw). On the 36th day, mice were taken down by anesthesia and followed by surgery to collect spermatozoa. The parameters for this observation were the concentration, motility, viability, morphology, and integrity of the spermatozoa membrane. The data obtained were analyzed statistically with One Way Anova and further tested with DMRT. The results showed that the treatment of robusta coffee leaf exstract in mice exposed to cigarette smoke had a significant effect (P<0,05) on the quality of spermatozoa.
DIAMETER OOSIT KELINCI (Lepus sp.) SETELAH DIBERI PAKAN KOMERSIAL DISUPLEMENTASI MINYAK HATI IKAN KOD Ni Gusti Ayu Manik Ermayanti; Gusti Ayu Sugi Wahyuni; I Gusti Ayu Manik Widhyastini
JURNAL WIDYA BIOLOGI Vol 10 No 02 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.204 KB) | DOI: 10.32795/widyabiologi.v10i02.409

Abstract

Minyak hati ikan kod sebagai sumber asam lemak tidak jenuh ganda mempunyai manfaat yang sangat beragam dan kompleks, di antaranya untuk kesehatan organ reproduksi. Penelitian ini bertujuan untuk mengetahui diameter oosit kelinci setelah diberi pakan komersial disuplementasi minyak hati ikan kod. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan empat perlakuan pakan, yaitu pakan komersial tanpa suplementasi minyak hati ikan kod (P0) sebagai kontrol, pakan komersial disuplementasi minyak hati ikan kod 3% (P1), pakan komersial disuplementasi minyak hati ikan kod 4,5% (P2), dan pakan komersial disuplementasi minyak hati ikan kod 6% (P3). Masing-masing perlakuan terdiri atas sepuluh ekor kelinci sebagai ulangan dan perlakuan diberikan mulai kelinci berumur 4 bulan sampai umur 6 bulan. Akhir perlakuan, kelinci dibedah dan selanjutnya dibuat preparathistologi ovarium. Parameter yang diamati adalah diameter oosit pada folikel primer, sekunder, tersier dan folekel praovulasi. Data yang diperoleh dianalisis dengan ANOVA, jika berbeda nyata diuji lanjut dengan DMRT. Hasil penelitian menunjukkan bahwa suplementasi minyak hati ikan kod dengan taraf yang berbeda pada pakan komersial berpengaruh nyata (P<0,05) terhadap diameter oosit kelinci. Dapat disimpulkan bahwa suplementasi minyak hati ikan kod pada taraf 4,5% pada pakan komersial mampu meningkatkan diameter oosit kelinci.
Blood Glucose Level and Pancreas Histological Section of Male Rabbits after Being Fed Commercial Feed Supplemented with Cod Liver Oil Ni Gusti Ayu Manik Ermayanti; Ida Bagus Made Suaskara; I Gusti Ayu Manik Widhyastini; Iriani Setyawati
JURNAL SAINS NATURAL Vol. 13 No. 1 (2023): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v13i1.441

Abstract

Fish oil contains eicosapentaenoic acid (E.P.A.) and docosahexaenoic acid (D.H.A.) which are beneficial for heart health, controlling blood glucose, reducing atherosclerosis, and lowering the risk of cancer. This study aims to determine the effect of commercial feed supplemented with cod liver oil on blood glucose levels and pancreatic histology of male rabbits. Twenty rabbits were divided into four groups, namely commercial feed supplemented with cod liver oil 3% (P1), 4.5% (P2), 6% (P3), and commercial feed without cod liver oil supplementation (control). Each group consisted of five rabbits, and the treatment was given for four weeks. The parameters observed were blood glucose levels in the 1st, 2nd, 3rd, 4th, and 5th weeks and the histology of the male rabbits’ pancreas. Data were analyzed by Anova. The results showed that in the 1st, 2nd, and 3rd weeks there was no significant difference (P>0.05). In contrast, in the 4th  and 5th weeks, there was a significant difference (P<0.05) between the control and treatment of cod liver oil supplementation on blood glucose levels in male rabbits, and the observational results on the histology of rabbits showed normal pancreatic histology. In conclusion, cod liver oil supplementation on commercial feed controlled blood glucose levels, and there was no disturbance in the histological structure of the pancreas of male rabbits.Keywords: fatty acid, glucose, cod liver oil, pancreas, rabbit
Integration Model of Nutritional Education, Tuina Massage and Psychomotoric Training (Gina Latrik) in Efforts for Food Security and Decreasing of Stunting in Toddler at UPT Puskesmas Klungkung I Juniarsana, I Wayan; Sukraniti, Desak Putu; Suriani, Ni Luh; Ermayanti, Ni Gusti Ayu Manik
Tropical Plantation Journal Vol 3, No 1 (2024): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v3i1.37

Abstract

Stunting is still a health problem faced by Indonesian children. The incidence of stunting according to Indonesian Nutrition Status Survey (SSGI) in 2021 was 24.4% and in 2022 it’s decrease to 21.6%. There were exceeds from WHO recommended requirement 20%. Holistic efforts from the government and society were needed to accelerate stunting reduction. This research aims to produce an integrated approach model such as nutrition education, tuina massage and psychomotor training (Gina Latrik) in accelerating to decrease of stunting rates. This type of research is true experimental. Subjects were divided into two groups, ie;  “Gina Latrik B” treatment group (balanced nutrition education, massage Tuina therapy with a frequency of 3–5 times/week and psychomotor training) and control group “Gina Latrik A” (balanced nutrition education, massage therapyTuinawith a frequency of 1–2 times/week and psychomotor training). The average knowledge level of toddler mothers after given the intervention was 83.66 ± 4.04% in the control group and 87.98 ± 5.88% in treatment group and significant difference with p=0.040 (p0.05). The results of research on macronutrient intake (energy, protein) in two groups showed significant differences (p0.05) and nutrient intake in Gina Latrik B was better than Gina Latrik A. Nutritional status results with height/age shows that the Z score = 2.17 ± 0.379 (Gina latrik A) dan 2.73 ± 0.452 for Gina Latrik B) with p=0.003 (p0.05), there was a significant difference on t-independent analysis. That means the Gina Latrik B was better than Gina Latrik A. Psychomotor development shows an increase in both groups, in Gina Latrik A there is an increase in psychomotor ability in the "appropriate" category of 33,30% to 60%. Meanwhile, in the Gina Latrik B group there was a sharp increase from 39.39% to 90.91%. Based on analysist independent showed a significant difference p = 0.003 (p0.005). Based on the results it can be prove that Gina Latrik B intervention was better for decreasing of stunting compared to Gina Latrik A.