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All Journal Jurnal Sain Veteriner Journal of Electronics, Electromedical Engineering, and Medical Informatics Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Kedokteran Hewan
Desem, Muhammad Ibrahim
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Jurnal Sain Veteriner

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Comparison of Morphology and Protein Profile of Acetone and PBS-Fixed Toxoplasma gondii Tachyzoites for Detection of IgG and IgM Seropositivity VALINATA, SISCA; Cahyaningsih, Umi; Nugraha, Arifin Budiman; Kurniawati, Dyah Ayu; Desem, Muhammad Ibrahim; Fong, Sulinawati
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.103155

Abstract

Toxoplasma gondii is an obligate intracellular protozoan that causes zoonotic diseases. The prevalence of toxoplasmosis in humans in Indonesia ranges from 9.7-70%. The parasite is difficult to detect in tissues; therefore, serological testing is the most common method for detecting antibodies against Toxoplasma. Antibodies are often used as the testing criteria for IgG and IgM antibodies. Previous research has shown that agglutination testing with acetone-fixed tachyzoites was only positive for acute infection. The aim of this study was to examine microscopic changes in acetone-fixed tachyzoites, determine their ability to detect IgG and IgM seropositivity, and detect specific proteins for IgG and IgM against toxoplasmosis. Tachyzoites were fixed with acetone (A) and PBS (P). After fixation, tachyzoites were prepared to observe the morphology and sonicated to obtain Soluble Toxoplasma Antigen (STA). STA was then subjected to SDS page and western blotting. The addition of aseton resulted in morphological and protein changes. Although changes occur, acetone-fixed tachyzoites can still react with IgG and IgM seropositivity. Protein bands that can be used as IgG seropositive markers in western blot testing are bands measuring 20, 24, 27, 73, and 110 kDa, and the combination of acetone fixation with anti-goat IgM conjugate will result in seropositive bands.
Co-Authors Adidharma, Peter Adiningsih, Setyo Andreas Wilson Setiawan Asmaria, Talitha Azmi, Zul Didik Tulus Subekti Fitrine Ekawasti, Fitrine Fong, Sulinawati Hawar, Ihtifazhuddin Hidayat, Yance Jacinda, Reica Diva Kurniawati, Dyah Ayu Menik Dwi Kurniatie Monica Dwi Hartanti, Monica Dwi Nona Rahmaida Puetri, Nona Rahmaida Nugraha, Arifin Budiman Poetranto, Anna Lystia Prasetya, Mustaqim Rina Isnawati, Rina Sadiah, Siti SAPUTRA, FERY SURAHMAN Umi Cahyaningsih Valinata, Sisca Yossy, Nebrisca Patriana