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Effect of Oven Drying Temperature on The Tanin Content of Bungur (Lagerstroemia speciosa Auct. non (L.) Pers) Leaves Upa, Nurul Fatmawati Pua; Kolobani, Matias Nataniel; Wardani, Nimas Prita R.K; Kale, Maria Laurenci Fanny Permata; Tunggal, Deif
Golden Ratio of Data in Summary Vol. 4 No. 1 (2024): November - April
Publisher : Manunggal Halim Jaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52970/grdis.v4i1.1001

A study was conducted on the effect of oven drying temperature on tannin content of bungur leaves (Lagerstroemia speciosa Auct. non (L.) Pers). Bungur leaves were picked, dry sorted, washed, wet sorted, and drained then oven dried at 30oC, 60(o)C, 90oC respectively. After drying, the samples were then measured for moisture content and then made into bungur leaf powder. Bungur leaf powder was then extracted by modified maceration using aquadem solvent. The extract obtained was tested qualitatively and quantitatively. Qualitative test results showed the presence of tannins. Quantitative tests using permanganometry, obtained tannin levels at drying temperatures of 30oC, 60oC, and 90oC were 7.27% ± 0.1429, 15.26% ± 1.0610, and 9.92% ± 0.7156, respectively. The highest tannin content was obtained at 60oC drying temperature.

Pengaruh Emulgator Polisorbat 60–Sorbitan 60 Terhadap Kestabilan Fisik Krim Pemutih Ekstrak Metanol Rimpang Lakka-Lakka (Curculigo orchioides Gaertn.). Kolobani, Matias Nataniel; Pua Upa, Nurul Fatmawati; Purnasari, Cahyani; Wardani, Nimas Prita Rahajengningtyas Kusuma; Kale, Maria Laurenci Fanny Permata
Archives Pharmacia Vol 7, No 1 (2025): ARCHIVES PHARMACIA
Publisher : Lembaga Penerbitan Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/ap.v7i1.8899

Mekanisme kerja bahan pemutih salah satunya adalah menghambat aktivitas enzim tyrosinase. Ekstrak metanol rimpang Lakka-Lakka (Curculigo orchioides Gaertn.) telah diteliti dapat menghambat aktivitas enzim tyrosinase dengan nilai IC50 10,2 bpj. Oleh karena itu dapat diformulasi menjadi krim pemutih yang stabil secara fisika kimia dan aman untuk digunakan. Penelitian ini bertujuan untuk mendapatkan formulasi krim pemutih tipe a/m dari ekstrak metanol rimpang Lakka-Lakka (Curculigo orchioides Gaertn.) yang stabil secara fisik dengan emulgator Polisorbat 60-Sorbitan 60.Pada penelitian ini rimpang Lakka-Lakka dimaserasi dengan menggunakan penyari metanol kemudian diuapkan hingga diperoleh ekstrak kental, kemudian dibuat sediaan krim pemutih tipe a/m dengan menggunakan emulgator Polisorbat 60–Sorbitan 60 dengan konsentrasi 1%, 2% dan 4%. Pengujian kestabilan fisik krim meliputi pengukuran viskositas, tetesan terdispersi, pengukuran pH, dan inversi fase sebelum dan setelah kondisi penyimpanan dipercepat selama 12 jam secara bergantian pada suhu 5oC dan 35oC sebanyak 10 siklus.Pengujian kestabilan fisik memperlihatkan krim yang menggunakan emulgator Polisorbat 60-Sorbitan 60 pada konsentrasi 1%, 2% dan 4 % stabil secara fisik karena viskositas, tetes terdispersi, pH tidak mengalami perubahan yang signifikan dan krim tidak mengalami inversi fase sebelum dan setelah penyimpanan dipercepat. Formula krim yang paling stabil yaitu krim dengan emulgator Polisorbat 60-Sorbitan 60 konsentrasi 4% karena berdasarkan hasil uji viskositas krim ini paling kental, dimana semakin kental suatu krim maka semakin mampu mencegah terjadinya penggabungan tetesan terdispersi dan sedimentasi atau kriming yang menghasilkan terjadi pemisahan fase.

Effect of Oven Drying Temperature on The Tanin Content of Bungur (Lagerstroemia speciosa Auct. non (L.) Pers) Leaves Upa, Nurul Fatmawati Pua; Kolobani, Matias Nataniel; Wardani, Nimas Prita R.K; Kale, Maria Laurenci Fanny Permata; Tunggal, Deif
Golden Ratio of Data in Summary Vol. 4 No. 1 (2024): November - April
Publisher : Manunggal Halim Jaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52970/grdis.v4i1.1001

A study was conducted on the effect of oven drying temperature on tannin content of bungur leaves (Lagerstroemia speciosa Auct. non (L.) Pers). Bungur leaves were picked, dry sorted, washed, wet sorted, and drained then oven dried at 30oC, 60(o)C, 90oC respectively. After drying, the samples were then measured for moisture content and then made into bungur leaf powder. Bungur leaf powder was then extracted by modified maceration using aquadem solvent. The extract obtained was tested qualitatively and quantitatively. Qualitative test results showed the presence of tannins. Quantitative tests using permanganometry, obtained tannin levels at drying temperatures of 30oC, 60oC, and 90oC were 7.27% ± 0.1429, 15.26% ± 1.0610, and 9.92% ± 0.7156, respectively. The highest tannin content was obtained at 60oC drying temperature.

Kemitraan Masyarakat Dalam Peningkatan Mutu dan Produksi VCO Mina Malole Rote Ndao Muntasir, Muntasir; Dean, Muhajirin; Kolobani, Matias Nataniel; Purnasari, Cahyani; Upa, Fatmawati Pua
Jurnal Abdimas Multidisiplin Vol. 4 No. 1 (2025): Oktober
Publisher : Penerbit Goodwood

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35912/jamu.v4i1.5290

Purpose: This community service program is designed to increase the quantity and quality of pure coconut oil production. through a partnership with Mitra Mina Malole. Methodology/approach: A participatory approach is implemented through training on VCO fermentation technology, mentoring on the use of appropriate tools, and regular implementation evaluations. The implementation team, comprising lecturers and students, collaborates directly with the partner community to transfer technology and monitor production outcomes. Results/findings: The community service program demonstrated an increase in production capacity, from 10–13 bottles per day to 20–25 bottles in 100 mL packaging, as well as improved product quality, resulting in a more hygienic and high-quality product. The product has obtained a P-IRT label and permit, and marketing has begun to expand, contributing to increased income and welfare for the local community. Conclutions: Community partnerships through appropriate technology and training have successfully improved the quality and production capacity of Virgin Coconut Oil in Ingguinak Village, increased family income, strengthened local business institutions, and expanded market access with more competitive and hygienic products. Limitations: The limited time for implementation means that ongoing support is still very much needed. Limited access to logistics and infrastructure in remote areas means that product distribution is not yet optimal. Contribution: This Program provides tangible socio-economic impacts for coconut farming communities. This program's recommendations can serve as a reference for developing broader community service policies and practices, supporting sustainable development, and strengthening local natural resource-based community economies.

Metabolite Profile of Boiled Ndusuk Leaves (Melastoma malabathricum L.) from Manggarai Based on GC-MS Analysis Nabuasa, Bernadeta Stien Julian; Dean, Muhajirin; Kolobani, Matias Nataniel; Neolaka, Yantus Aristarkus B.
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): in Progress
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12888

Ndusuk leaves (Melastoma malabathricum L.) are traditionally used by the Manggarai community to reduce the fatty appearance of pork dishes, yet the molecular basis of this practice has not been investigated using a GC-MS-based metabolomic approach. This study compared the metabolite profiles of Ndusuk leaf decoctions prepared without and with pork. Dried leaves (35 g) were decocted in 2.5 L of water at 100°C for 30 min under two treatments: leaves only (control) and leaves with 100 g pork (sample), each performed in triplicate (n = 3). Metabolites were analyzed by GC-MS and tentatively identified using the NIST library (similarity index ≥80%). The control contained 118 tentatively identified metabolites, whereas the pork-treated extract contained 126. Boiling with pork increased cis-vaccenic acid (+4.07%) and palmitic acid (+1.92%), while decreasing melezitose (−2.80%) and glycerol (−0.73%). These findings demonstrate that cooking with pork alters the metabolite profile of Ndusuk leaf decoctions without providing evidence of direct fat reduction. The observed metabolite shifts may suggest possible phenolic–lipid interactions, antioxidant-related processes, and Maillard reaction-associated changes; however, these mechanisms require confirmation using targeted metabolomics and authentic standards. This study provides the first metabolomic evidence supporting the traditional culinary use of Ndusuk leaves and establishes a scientific basis for Manggarai pork-cooking practices.

Analysis of the GC-MS Volatile Compound Profile and Antibacterial Activity of 95% Ethanolic Extract of Siam Weed (Chromolaena odorata L.) Leaves from Kupang Regency Against Staphylococcus aureus Saingo, Mathias Endrico; Regaletha, Tadeus A. L.; Purnasari, Cahyani; Kolobani, Matias Nataniel
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): in Progress
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12895

The need for new antibacterial medicines derived from natural sources is highlighted by the dangerous bacterium Staphylococcus aureus, which causes a variety of diseases and is increasingly linked to antibiotic resistance. The leaves of Chromolaena odorata (L.) contain a variety of bioactive compounds that may have antibacterial qualities. This study aimed to characterize the metabolite profile of the 95% ethanol extract of C. odorata leaves using gas chromatography–mass spectrometry (GC-MS) and evaluate its antibacterial activity against S. aureus. This true experimental post-test-only control group study was conducted from January to May 2026 using leaves collected from Mata Air Village, Kupang Tengah District, Kupang Regency, East Nusa Tenggara, Indonesia. Antibacterial assays were performed in four replicates using the disk diffusion method at extract concentrations of 5–25%. GC-MS identified 118 compounds, with ethyl palmitate, α-linolenic acid, phytol, stigmasterol, squalene, caryophyllene oxide, and α-muurolol as dominant metabolites that may contribute to the observed antibacterial activity based on previous studies. S. aureus was inhibited by the extract in a concentration-dependent manner; inhibition zones increased from 8.03 ± 0.04 mm (5%) to 11.41 ± 0.13 mm (25%), and there were significant differences between treatments (one-way ANOVA, p < 0.001). This study shows how GC-MS metabolite profiling and antibacterial assessment of the 95% ethanol extract of C. odorata can be combined in a unique way. These results suggest that the extract has antibacterial action against S. aureus and could be a source of natural antibacterial chemicals.

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