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All Journal Majalah Kedokteran Bandung Jurnal Riset Kimia Chimica et Natura Acta Kimia Padjadjaran
Hafiz, Ersanda
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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Dysobinol Extracted from Chisocheton Macrophyllus Triggers Proliferation Inhibition, Potential Apoptosis, and Cell Cycle Arrest of He La Cancer Cell Lines Gaffar, Shabarni; Hafiz, Ersanda; Wiraswati, Hesti Lina; Ishmayana, Safri; Nurlelasari, Nurlelasari
Majalah Kedokteran Bandung Vol 56, No 1 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3249

Abstract

Dysobinol is a new limonoid from C. macrophyllus seeds reported to have an anticancer activity. This study aimed to determine the cytotoxic activity of Dysobinol against HeLa cancer cell lines and evaluate its mechanism of action by determining the expression level of several carcinogenesis genes related to apoptosis and cell cycle. In this experimental study, the cytotoxic activity was determined using the MTS assay and gene expression by real-time reverse transcriptase PCR. The result shows that Dysobinol has an anticancer activity in a dose and time-dependent manner against HeLa cells and was categorized as toxic with IC50 values of 52.92, 52.70, and 14.96 μg/ml for 24, 48, and 72 hours, respectively. Dysobinol significantly increased the expression of Bax, Cas-8, and Cas-3 and decreased the expression of Cyc D1 at both doses (IC50 and 2x IC50) but only high doses (2x IC50) could affect Cas9 and NF-κB expressions, indicating that Dysobinol can induce apoptosis via the extrinsic pathway and inhibits the cell cycle through the Cyc D1 regulator. Dysobinol has the potential to be developed as a chemotherapy drug or an adjuvant agent for cervical cancer treatment.
Pengaruh Penambahan Psyllium dan Susu Full Cream terhadap Kadar Air dan Abu Produk Yoghurt Safari, Agus; Devi, Puspitasari Rosiana; Horasio, Debora Tamaris; Nurrusyda, Fajriana Shafira; Anggraeni, Nenden Indrayati; Fadhlillah, Muhammad; Soedjanaatmadja, Ukun M. S.; Ishmayana, Safri; Hafiz, Ersanda
Kimia Padjadjaran Vol 1, No 2 (2023)
Publisher : Kimia Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Yogurt termasuk pangan fungsional, yaitu salah satu pangan yang tidak hanya memenuhi kebutuhan tetapi memiliki fungsi untuk kesehatan tubuh. Yogurt dapat diperoleh dari fermentasi susu oleh bakteri asam laktat yang akan menghasilkan aroma khas, tekstur semi padat dan halus, kompak serta rasa asam yang segar. Kandungan gizi pada yogurt lebih tinggi dibandingkan susu murni karena adanya penambahan zat-zat gizi hasil sintesa mikroba dan kandungan gizi dari mikroba itu sendiri seperti protein, vitamin, asam folat, riboflavin, kalsium serta fosfor. Salah satu karakteristik kualitas yogurt yang paling penting adalah tekstur dan tampak keseluruhan yang sesuai dengan tingkat sineresis yang rendah. Beberapa penelitian menunjukkan bahwa cara untuk memperbaiki keadaan fisik, tekstur, dan reologi dari yogurt adalah dengan menambahkan berbagai bahan penstabil ke dalam susu. Selain penstabil, komponen susu yang berpengaruh pada tekstur yoghurt adalah jumlah lemak yang terkandung didalamnya. Psyllium digunakan bahan penstabil dikarenakan polisakarida pada psyllium ini dapat meningkatkan konsistensi dan stabilitas sistem melalui pembentukan gel yang kuat. Kemudian untuk menambah lemak digunakan susu full cream. Kombinasi kedua variable ini dioptimasi menggunakan Response Surface Method-Central Composite Design (RSM-CCD), kemudian dianalisa kadar lemak dan kadar abunya untuk mengetahui pengaruh psyllium dan susu full cream terhadap sineresis. Melalui penelitian didapatkan hasil kadar air sebesar 70,87% dan kadar abu sebesar 3,96%.
Determination of Apoptosis Level of Hela Cells Treated with Disobinol Compound from Chisocheton Macrophyllus Plant Hafiz, Ersanda; Albayyinah, Dyandra Hera; Melati, Rima; Herlina, Tati; Permadi, Nandang; Amalia, Riezki; Nurlelasari, Nurlelasari; Gaffar, Shabarni
Jurnal Riset Kimia Vol. 16 No. 1 (2025): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v16i1.772

Abstract

Disobinol is a limonoid compound from the seeds of the Chisocheton macrophyllus plant that has been reported to have anticancer activity against cervical cancer cell lines. Cervical cancer is a type of cancer caused by infection with Human papillomavirus (HPV) types 16 and 18, leading to the transformation of normal cervical epithelial cells into cancerous cells. Previous studies show that Disobinol has a cytotoxic effect on HeLa cells with the IC50 value 52,92 μg/mL (24 hours’ incubation). This study aims to determine the level of apoptosis in HeLa cells treated with Disobinol and observe the DNA fragmentation in HeLa cells undergoing apoptosis. The HeLa cells were treated with Disobinol at concentrations of 26,5 μg/mL (1/2 IC50), 52,3 μg/mL (IC50), and 104,6 μg/mL (2x IC50) and incubated for 24 hours. The level of apoptosis was analyzed by using flow cytometry, and the DNA fragmentation pattern was analyzed by agarose gel electrophoresis. The results showed that Disobinol induces early apoptosis in HeLa cells, although the difference in the percentage of cell death is not very significant, which were 37.05%, 37.7%, and 41.60%, respectively. The DNA fragmentation pattern in HeLa cells treated with various concentrations of Disobinol was also observed on agarose gel. Therefore, Disobinol has the potential to be used as a chemotherapeutic drug or as a lead compound for the treatment of cervical cancer.
Statistical Validation of The Microplate Reader for Antioxidant Activity Measurement using The DPPH Assay Rismawati, Rismawati; Kurnia, Dikdik; Hafiz, Ersanda; Zein, Muhammad Ihda H. L.
Chimica et Natura Acta Vol 13, No 3 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n3.61575

Abstract

Antioxidants research has been garnering increased interest among researchers, particularly in the fields of medicine and health, focusing on both natural and synthetic antioxidants. The DPPH method is the most used approach for antioxidant analysis due to its efficiency, simplicity, and accuracy. This study aims to validate the sensitivity of the microplate reader compared to UV-Vis spectrophotometer, both used for measuring absorbance in the DPPH antioxidant test. The samples used in this study include ascorbic acid, gallic acid, and quercetin standards. Statistical validation in the DPPH antioxidant test includes precision testing, T-test, and % recovery test. Based on the statistically analyzed results, the T-test values for ascorbic acid, gallic acid, and quercetin standards were 0.86, 1.52 and 0.20, respectively, all of which are less than the t-table value of 1.72. The T-test values being less than the t-table value indicates that there is no significant difference between the two methods, UV-Vis spectrophotometer and microplate reader. Meanwhile, the precision test (Horrat)r values for the two methods were 0.52, 0.33, and 0.34, respectively. These precision values (Horrat)r fall within the 0.3-1.5 range, indicating acceptable precision. Additionally, the % recovery test for gallic acid showed values in the 90-100% range, indicating that both methods possess good sensitivity. As a result, a microplate reader is equally reliable yet more practical (faster, high-throughput, smaller volumes), especially valuable for labs with limited resources.
Co-Authors Agus Safari Albayyinah, Dyandra Hera Amalia, Riezki Anggraeni, Nenden Indrayati Devi, Puspitasari Rosiana Dikdik Kurnia Fadhlillah, Muhammad Hesti Lina Wiraswati Horasio, Debora Tamaris Nurlelasari Nurlelasari Nurrusyda, Fajriana Shafira Permadi, Nandang R. Ukun M.S. Soedjanaatmadja Rima Melati Rismawati Rismawati Safri Ishmayana Shabarni Gaffar -, Shabarni Gaffar Tati Herlina Zein, Muhammad Ihda H. L.