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All Journal Majalah Kedokteran Bandung Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Teknologi Informasi dan Ilmu Komputer Jurnal Riset Kimia Global Medical and Health Communication Chimica et Natura Acta Jurnal Endurance: Kajian Ilmiah Problema Kesehatan Jurnal Abdimas Kartika Wijayakusuma The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy

Hesti Lina Wiraswati

Universitas Padjadjaran

Author-ID : 986636

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Meningkatkan Keterampilan Kader Kesehatan dalam Melakukan Pemeriksaan Payudara Sendiri (SADARI) Lina Wiraswati, Hesti; Ekawardhani, Savira; Windria, Sarasati; Faridah, Lia
Jurnal Endurance Vol 4, No 2 (2019): Jurnal Endurance : Kajian Ilmiah Problema Kesehatan
Publisher : Lembaga Layanan Pendidikan Tinggi (LLDIKTI) Wilayah X

Breast cancer is one of the most common cancer types suffered by women in the world. In 2018, the International Agency for Research on Cancer (IARC) estimated number of cancer cases in Indonesia was 42.1 per 100000 females, while the death rate was 17 per 100000 females. These conditions are worrisome and call for serious handling. For this purpose, the effectiveness of patient treatment and life quality must be improved. Therefore, this study aims to improve the knowledge and skills of breast cancer early detection. The method used is health training through audio-visual devices and demonstration. Qualitative and quantitative analysis was carried out using paired t-test. The sampling data was acquired by purposive sampling method using a questionnaire. The result showed that first, majority of health cadres (97%) had less knowledge about BSE, before the training was carrying out. Second, lack of knowledge about BSE apparently did not prevent health cadres from doing BSE (28%). Third, BSE training improves the skills of health cadres with an average increase of 31%. Fourth, the training has also increased the knowledge of all cadres about BSE with an average increase of 29%. In addition, all cadres stated that they would disseminate their BSE knowledge and skills to their families and others. Kanker payudara merupakan salah satu jenis kanker yang paling banyak di derita oleh wanita di dunia. International Agency for Reseach on Cancer (IARC) pada tahun 2018 memperkirakan insiden kanker payudara di Indonesia sebesar 42.1 per 100 ribu wanita, sedangkan angka kematiannya sebesar 17 per 100 ribu penduduk. Situasi seperti ini sangat mengkhawatirkan dan perlu penanganan serius dari berbagai pihak. Dengan demikian penelitian ini bertujuan untuk meningkatkan keterampilan kader kesehatan dalam melakukan pemeriksaan payudara sendiri (SADARI). Metode yang dilakukan adalah dengan melakukan pelatihan melalui perangkat audio visual dan alat peraga. Analisis kualitatif dan kuantitatif dilakukan menggunakan uji t-test berpasangan. Pengambilan sampel dilakukan secara purposive sampling dengan cara penetapan besar sampel berdasarkan jumlah kebutuhan minimal dengan menggunakan kuesioner. Hasil penelitian ini menunjukkan bahwa pertama, mayoritas kader kesehatan memiliki pengetahuan kurang tentang SADARI (97%). Kedua, pengetahuan yang kurang tentang SADARI rupanya tidak menghalangi kader kesehatan untuk melakukan SADARI (28%). Ketiga, pelatihan SADARI meningkatkan keterampilan kader kesehatan dengan rata-rata peningkatan 31%. Keempat, pelatihan SADARI juga meningkatkan pengetahuan kader dengan rata-rata peningkatan 29%. Disamping itu, semua kader menyatakan bahwa mereka akan menyebarkan pengetahuan dan keterampilan SADARI yang mereka miliki kepada keluarga dan orang lain

PENINGKATAN PENGETAHUAN KANKER PAYUDARA PADA MASYARAKAT DESA RANCAMANYAR KABUPATEN BANDUNG Hesti Lina Wiraswati; Savira Ekawardhani; Sarasati Windria; Lia Faridah; Syafrizal Saragih; Muhammad Saifuddin B. S. Arif; Meri Alex Sandra; Astri Gloria Larwuy; Dini Oktaviani; Yasfira Aradella; Salma Nur Raidah
Dharmakarya Vol 7, No 4 (2018): Desember
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/dharmakarya.v7i4.19051

Meningkatnya kasus kanker dan kematian akibat kanker di Indonesia dari tahun ke tahun perlu mendapatkan perhatian serius. Faktor penting yang berkontribusi pada peningkatan kejadian ini adalah kurangnya sosialisasi tentang kanker, penyebab kanker dan aktivitas-aktivitas penurun resiko kanker, disamping kurang efektifnya pengobatan dan upaya peningkatan kualitas hidup penderita. Oleh karena itu penelitian ini bertujuan untuk meningkatkan wawasan masyarakat tentang penyakit kanker, termasuk penyebab kanker dan faktor-faktor penurun resiko kanker sehingga diharapkan akan meningkatkan kesadaran dan kewaspadaan masyarakat agar terhindar dari penyakit ini. Kegiatan yang dilakukan dalam penelitian ini adalah berupa penyuluhan yang berbasis metode pendidikan masyarakat dengan berbagai macam aktivitas diantaranya ceramah, diskusi, nonton video, dan tanya jawab berhadiah. Pre-test dan post-test digunakan sebagai alat ukur untuk melihat ketercapaian tujuan. Hasilnya pertama, terjadi peningkatan pemahaman peserta rata-rata 23%, dengan rentang pemahaman 34%-89%. Kedua, selama diskusi peserta menyatakan sikapnya untuk bergaya hidup sehat agar memperkecil resiko terkena kanker payudara. Hasil-hasil tersebut menunjukkan bahwa penyuluhan yang dilakukan mampu meningkatkan pemahaman masyarakat tentang kanker dan memunculkan kesadaran masyarakat untuk berpola hidup sehat sebagai bentuk upaya pencegahan kanker payudara.

Knowledge Level towards Breast Cancer and Breast Self-Examination among Medical Students of Indonesia Andri Rezano; Marhendra Satria Utama; Hesti Lina Wiraswati; Savira Ekawardhani; Melia Juwita Adha; Nurul Mufliha Patahuddin; Veronica Oladitha Siagian; Siti Silvia Nur Shofa Shamantri; Erlangga Ing Geni Bisma Pratama; Liana Awalia Lutfunnisa; Asep Wiryasa; Hansen Wangsa Herman
Global Medical & Health Communication (GMHC) Vol 10, No 1 (2022)
Publisher : Universitas Islam Bandung

Breast cancer is a life-threatening disease among Indonesian women. The etiology of breast cancer is still uncertain, and therefore adequate primary prevention is difficult. Early diagnosis improves cancer prognosis while also reducing medical costs, substantially reducing mortality rates. Knowledge and awareness of breast cancer risk factors and their screening may help women take preventive measures. The community service program aimed to assess the level of understanding of undergraduate medical students on breast cancer and breast self-examination (BSE). It was a one-group pretest-postest quasi-experimental study to measure the level of knowledge of 100 medical students in Indonesia from August 20th, 2020, to February 27th, 2021. The participants were asked 36 questions, which was an adaptation of previous questionnaires. A mini-lecture managed the program that focuses on risk factors and early detection. Furthermore, pretest and post-test were conducted to analyze the knowledge level before and after the dissemination. The participants included in this study were mostly 20 years and above (62%). The average recognition of breast cancer and its early detection was not high. The knowledge of breast cancer among medical students was found to be moderate. The efficacy of dissemination among medical students was apparent in knowledge change. However, good cancer awareness, especially breast cancer, needs to be established and integrated through effective cancer educational programs in the medical curricula. Hence, modification of the medical curriculum through extensive training on breast cancer preventive measures and early diagnosis is required.

Aktivitas Antioksidan dan Sitotoksik Terhadap Sel Kanker HeLa dari Ekstrak Daun Vernonia amygdalina (Asteraceae) Shabarni Gaffar; Muhammad Yudha Nugraha; Ersanda Hafiz; Hesti Lina Wiraswati; Tati Herlina
Chimica et Natura Acta Vol 10, No 1 (2022)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v10.n1.36779

Vernonia amygdalina atau dikenal sebagai daun afrika merupakan salah satu tanaman obat yang berpotensi untuk dikembangkan sebagai nutraceutical, seperti antidiabetes, antimalaria dan antikanker. Penelitian ini bertujuan menentukan aktivitas antioksidan dan aktivitas sitotoksik ekstrak daun V. amygdalina terhadap sel kanker serviks HeLa, serta menentukan kelompok senyawa yang terdapat dalam ekstrak daun V. amygdalina dengan aktivitas sitotoksik dan antioksidan tertinggi melalui penapisan fitokimia. Metode yang digunakan pada penelitian ini adalah ekstraksi dan fraksinasi menggunakan pelarut metanol, etil asetat, dan n-hexana. Semua fraksi diuji aktivitas antioksidannya menggunakan metoda DPPH dan aktivitas sitotoksiknya terhadap sel kanker serviks HeLa menggunakan metode MTS, serta penapisan fitokimia untuk menentukan kelompok senyawa alkaloid, terpenoid, steroid, dan flavonoid. Hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan paling tinggi, IC50 ekstrak metanol, etil asetat dan n-heksana berturut-turut adalah: 25,27; 17,89; 213,4 ppm. Hasil uji aktivitas sitotoksik menunjukkan bahwa ekstrak metanol, etil asetat dan n-heksana daun V. amygdalina bersifat kurang aktif terhadap sel kanker serviks HeLa dengan IC50 berturut-turut: 519,42; 249,18 dan 367,24 ppm. Ekstrak etil asetat yang mengandung aktivitas antioksidan tertinggi mengandung kelompok senyawa flavonoid, alkaloid, steroid, dan terpenoid, sehingga berpotensi untuk dimanfaatkan sebagai agen kemopreventif berdasarkan aktivitas antioksidannya.

Physalis angulata Linn. As a Potential Liver Antifibrotic Agent In Rats. Bestari, Muhammad Begawan; Rohmawaty, Enny; Rosdianto, Aziiz Mardanarian; Usman, Hermin A.; Saragih, Winda A. M.; Zuhrotun, Ade; Hendriani, Rini; Wardhana, Yoga Windhu; Ekawardhani, Savira; Wiraswati, Hesti Lina; Agustanti, Nenny; Dewi, Sumartini; Wijaya, Muhammad Palar
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy Vol 24, No 3 (2023): VOLUME 24, NUMBER 3, December, 2023
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/2432023206

Background: No drug with a liver antifibrotic effect for treating non-alcoholic fatty liver disease (NAFLD) has been approved. Physalis angulata Linn., better known to Indonesian as ciplukan, has natural abilities in various metabolic and inflammatory diseases. This study aims to determine the effect of ethyl acetate fraction of P. angulata in the NAFLD rat model by examining alanine aminotransferase (ALT), cholesterol levels, and liver histopathological features, which are methods to evaluate the course of the disease and the potential antifibrotic effect.Method: This research is an in vivo study on male Wistar rats conducted at the Animal Laboratory, Faculty of Medicine, Universitas Padjadjaran, from September to November 2020. Rats were grouped randomly into seven groups of 5 each. The NAFLD models were created by giving a diet containing 20% margarine for four weeks. The intervention groups were given vitamin E, ethyl acetate fraction of P. angulata, and both combinations. The statistical analysis examined differences in each group based on their histopathological features, ALT, and cholesterol levels.Results: Histopathological results in the group given P. angulata at a dose of 0.32 mg resembled normal liver, and the ALT level was similar to vitamin E. The administration of P. angulata at 0.16 mg dose improved cholesterol levels.Conclusions: P. angulata ethyl acetate fraction at a dose of 0.32 mg improved the histopathological and serum ALT levels in the NAFLD rat model, which could be the basis for the mechanism of P. angulata's antifibrotic ability in NAFLD conditions.

Dysobinol Extracted from Chisocheton Macrophyllus Triggers Proliferation Inhibition, Potential Apoptosis, and Cell Cycle Arrest of He La Cancer Cell Lines Gaffar, Shabarni; Hafiz, Ersanda; Wiraswati, Hesti Lina; Ishmayana, Safri; Nurlelasari, Nurlelasari
Majalah Kedokteran Bandung Vol 56, No 1 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3249

Dysobinol is a new limonoid from C. macrophyllus seeds reported to have an anticancer activity. This study aimed to determine the cytotoxic activity of Dysobinol against HeLa cancer cell lines and evaluate its mechanism of action by determining the expression level of several carcinogenesis genes related to apoptosis and cell cycle. In this experimental study, the cytotoxic activity was determined using the MTS assay and gene expression by real-time reverse transcriptase PCR. The result shows that Dysobinol has an anticancer activity in a dose and time-dependent manner against HeLa cells and was categorized as toxic with IC50 values of 52.92, 52.70, and 14.96 μg/ml for 24, 48, and 72 hours, respectively. Dysobinol significantly increased the expression of Bax, Cas-8, and Cas-3 and decreased the expression of Cyc D1 at both doses (IC50 and 2x IC50) but only high doses (2x IC50) could affect Cas9 and NF-κB expressions, indicating that Dysobinol can induce apoptosis via the extrinsic pathway and inhibits the cell cycle through the Cyc D1 regulator. Dysobinol has the potential to be developed as a chemotherapy drug or an adjuvant agent for cervical cancer treatment.

New Custom Primers for the Detection of SARS-CoV-2 using the Singleplex rRT‒PCR SYBR Green-Based Method with the NSP10 and N genes as Targets Gaffar, Shabarni; Shabrinna, Hanif; Putri, Rafika; Wiraswati, Hesti Lina; Hartati, Yeni Wahyuni; Ishmayana, Safri; Faridah, Lia; Ekawardhani, Savira
Chimica et Natura Acta Vol 13, No 1 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n1.53493

Although COVID-19 is no longer a global health emergency, rapid, sensitive, and specific detection tests are still needed. In this study, we developed a cost-effective test, the SYBR Green-based rRT‒PCR kit, using new custom primers targeting the N and NSP10 genes of the SARS-CoV-2 virus. The specificity of the designed primers was determined through agarose gel electrophoresis. A standard curve generated from a ten-fold dilution of SARS-CoV-2 RNA was used to determine the efficiency and sensitivity of the kit. Validation of this protocol was carried out on ten clinical specimens. As expected, the results showed that the N and NSP10 gene primers produced 134 and 161 bp products, respectively. The limits of detection and limit of quantification with N gene primers were 7.74 and 23.46 copies/μL, respectively, and those with the NSP10 gene primers were 4.69 and 14.21 copies/μL, with a PCR efficiency of 102.5% and 110.6%, respectively. The validation results with clinical specimens revealed that seven samples were true-positive for COVID-19 (Ct range 15.09–21.33), and three were confirmed to be true-negative. Costs associated with COVID-19 patient testing can be anticipated to decrease with the use of custom primers for the detection of SARS-CoV-2 via the use of the singleplex rRT‒PCR mix SYBR Green.

Edukasi dan Sosialisasi Pencegahan Skabies di Pondok Pesantren Modern Al-Aqsha Kabupaten Sumedang Akbari, Anamika Lingga Anjani; Laurence, Christopher; Permana, Noah Jefferson; Faiza, Tiara; Florine, Angela Chrisabel; Permatasari, Priska Dewi; Sukamto, Charissa Otniel; Adesandika, Egidia Listi; Suharman, Khuznul Khatimah; Fernanda, Keisha Arizka; Wiraswati, Hesti Lina
Jurnal Abdimas Kartika Wijayakusuma Vol 6 No 3 (2025): Jurnal Abdimas Kartika Wijayakusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v6i3.759

Skabies merupakan penyakit yang umum ditemukan di negara beriklim tropis, khususnya Indonesia. Kementerian Kesehatan Republik Indonesia mengungkapkan bahwa prevalensi skabies bernilai sekitar 8,5-9% dan menduduki peringkat 3 dari 12 penyakit kulit yang sering terjadi di Indonesia. Salah satu lokasi yang sering dilaporkan memiliki kasus skabies dengan frekuensi cukup tinggi adalah pondok pesantren. Tingkat kepadatan, faktor pencahayaan, akses ventilasi, serta penerapan pola hidup bersih dan sehat (PHBS) menjadi faktor fundamental penyebaran skabies, termasuk di pesantren. Hal tersebut menjadi landasan untuk dilaksanakannya program pengabdian masyarakat kepada para siswa dan siswi pesantren agar dapat mengetahui langkah preventif serta kuratif terhadap skabies. Edukasi yang dilakukan berupa penyuluhan skabies terhadap siswa dan siswi Pondok Pesantren Al-Aqsha di Kabupaten Sumedang, Jawa Barat. Pengisian kuesioner juga dilakukan untuk mengetahui gambaran PHBS di lingkungan pesantren. Pengukuran pengetahuan partisipan dilakukan dengan mengadakan pre-test dan post-test yang diisi oleh 91 responden, terjadi peningkatan pengetahuan santri dari 73% menjadi 82,14%. Hal tersebut menunjukkan bahwa metode penyuluhan dapat meningkatkan pengetahuan partisipan. Selain itu, secara umum santri Ponpes Al-Aqsha memiliki perilaku yang baik dalam menjaga kebersihan diri. Harapannya, tindakan preventif serta kuratif terkait skabies dapat ditingkatkan baik di tingkat individu maupun lembaga sehingga dapat mengurangi prevalensi skabies khususnya di pondok pesantren.

Informasi Dini Terhadap Penyakit Kanker Payudara berbasis Telepon Pintar Wiraswati, Hesti Lina; Ekawardhani, Savira; Windria, Sarasati; Khaq, Arina Al; Nurfitri, Fauzia; Wahyuni, Dini; Rachmawati, Nadya; Baasin, Rusdi Toriq; Arimdayu, Annissa Retno; Arimdayu, Annisa Retno
Jurnal Teknologi Informasi dan Ilmu Komputer Vol 9 No 4: Agustus 2022
Publisher : Fakultas Ilmu Komputer, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25126/jtiik.2022933182

Latar Belakang: Deteksi dini kanker payudara akan memberikan tingkat kesembuhan lebih tinggi bagi penderita jika penderita datang lebih dini ketika terdapat gejala adanya kanker payudara. Namun, masyarakat Indonesia cenderung datang ke pusat kesehatan dalam kondisi kanker stadium lanjut sehingga sulit diobati. Ketersediaan layanan informasi tentang kanker payudara, dan pemeriksaan mandiri berbasis telepon pintar menjadi kebutuhan saat ini. Oleh karena itu diperlukan sosialisasi yang benar tentang kanker. Salah satu alat sosialisasi yang dianggap efektif adalah media digital. Di Indonesia, aplikasi berbasis telepon pintar sudah dibuat untuk kanker serviks, tetapi untuk kanker payudara hanya tersedia dalam bentuk hologram berisi simulasi gerakan Periksa Payudara Sendiri (SADARI). Aplikasi hologram ini belum menyertakan informasi tentang kanker payudara secara komprehensif. Tujuan: Menyediakan layanan informasi tentang kanker payudara, dan penjelasan serta simulasi bagaimana memeriksa secara mandiri yang berbasis menggunakan aplikasi berbasis telepon pintar. Metode: Metode penelitian terdiri atas desain aplikasi, pengumpulan asset sebagai sumber database, validasi aplikasi, dan uji coba aplikasi kepada 100 responden menggunakan teknik purposive sampling. Evaluasi kelayakan aplikasi dilakukan menggunakan kuesioner pre-test dan post-test. Hasil: Aplikasi berhasil dibuat dan dapat diunduh melalui google play store. Aplikasi ini membantu meningkatkan pengetahuan responden tentang kanker dari rata-rata 43,38% menjadi 77,72%. Sebesar 34.6% responden setuju dan 61.8% sangat setuju bahwa aplikasi ‘Be Care’ berguna dan bermanfaat. Kesimpulan: Aplikasi “Be Care” dinilai layak untuk digunakan dalam meningkatkan pengetahuan, kesadaran, dan keterampilan terkait deteksi dini kanker payudara. Aplikasi berbahasa Indonesia dengan status ‘free access’ ini diharapkan menjadi salah satu media efektif yang berkontribusi dalam menurunkan jumlah kasus baru dan kematian akibat kanker. AbstractBackground: An early breast cancer detection will provide a higher cure rate for sufferers if the patient arrives early when there are symptoms of breast cancer. However, Indonesians tend to come to the health centres with an advance condition, which it makes difficult to be treated. The availability of information about breast cancer and smartphone-based self-examination is a necessity at this time. Therefore, we need the correct socialization. One of the socialization tools that are considered effective is digital media. In Indonesia, smartphone-based application has been made for servical cancer, but it is only available in hologram containing of Breast Self-Examination (BSE) for breast cancer. This does not include comprehensive information about breast cancer. Objective: To provide information service about breast cancer, and an explanation also simulation of how to do BSE based on a smartphone-based app. Methods: The research consists of application design, collection of assets as a database source, application validation, and testing to 100 respondents using purposive technique sampling. The evaluation for feasibility of the app was carried out using pre-test and post-test questionnaire. Results: The app was successfully created and can be downloaded via Google Playstore. This app helps increase knowledge of the respondents about cancer from an average 43.38% to 77.22%. As much as 34.6% of respondents agree and 61.8% strongly agree that the app “Be Care” is useful. Conclusion: "Be Care" app is considered feasible to be used in increasing knowledge, awareness, and skills related to early detection of breast cancer. This Indonesian language app with “free access” is expected to be one of the effective media contributing to reducing the number of new cases and death due to cancer.

In-house Multiplex rRT-PCR Assay for Sars-Cov2 Detection in Indonesia using a new primer design Gaffar, Shabarni; Putri, Rafika Nanda; Shabrinna, Hanif; Supriyadi, Isma Yustifania; Wiraswati, Hesti Lina; Ekawardani, Savira; Ishmayana, Safri; Hartati, Yeni Wahyuni; Faridah, Lia
Jurnal Riset Kimia Vol. 16 No. 1 (2025): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v16i1.781

During the COVID-19 pandemic, we attempted to develop an in-house rRT-PCR kit, utilizing custom primers targeting NSP14 and RdRp, with the RPP30 gene as an internal control. This kit will support Indonesian independence in enhancing COVID-19 diagnostics. The primer and probe were designed by a bioinformatics tool, determining primer specificity and sensitivity, optimizing probe concentrations, establishing LoD (Limit of Detection), LoQ (Limit of Quantification), and rRT-PCR efficiency, multiplex testing of the rRT-PCR kit on clinical samples, and testing the kit's stability. The in-house rRT-PCR kit can detect NSP14, RdRp, and RPP30 genes. The optimal concentrations for the NSP14, RdRp, and RPP30 probes are 1 μM, 1.5 μM, and 1.5 μM, respectively. The LoD and LoQ for the NSP14 are 0.22 ng/μL and 0.67 ng/μL, and for the RdRp are 1.08 ng/μL and 3.28 ng/μL. The rRT-PCR efficiencies for the NSP14, RdRp, and RPP30 are 80.3%, 100.6%, and 106%, respectively. Detection of ten clinical samples, comprising seven true positive and three true negative samples, showed Ct (Cycle threshold) values of 28–31 for the RPP30 gene, Ct 21–27 for the RdRp gene, and Ct 30–34 for the NSP14 gene. Stability testing of the rRT-PCR kit demonstrated promising results, where the kit stored at -20°C for seven days showed almost no difference in Ct values. This in-house multiplex rRT-PCR will support Indonesian independence in enhancing COVID-19 diagnostics, providing a dependable method for detecting SARS-CoV-2 in clinical samples.

Co-Authors Ade Zuhrotun Adesandika, Egidia Listi Akbari, Anamika Lingga Anjani Andri Rezano Arimdayu, Annisa Retno Arimdayu, Annissa Retno Asep Wiryasa Astri Gloria Larwuy Baasin, Rusdi Toriq Dewi, Sumartini Dini Oktaviani Dini Wahyuni Ekawardani, Savira Enny Rohmawaty Erlangga Ing Geni Bisma Pratama Ersanda Hafiz Faiza, Tiara Fernanda, Keisha Arizka Florine, Angela Chrisabel Hafiz, Ersanda Hansen Wangsa Herman Khaq, Arina Al Laurence, Christopher Lia Faridah Liana Awalia Lutfunnisa Marhendra Satria Utama Melia Juwita Adha Meri Alex Sandra Muhammad Begawan Bestari Muhammad Saifuddin B. S. Arif Muhammad Yudha Nugraha Nenny Agustanti Nurfitri, Fauzia Nurlelasari Nurlelasari Nurul Mufliha Patahuddin Permana, Noah Jefferson Permatasari, Priska Dewi PUTRI, RAFIKA Putri, Rafika Nanda Rachmawati, Nadya Rini Hendriani Rosdianto, Aziiz Mardanarian Safri Ishmayana Salma Nur Raidah Saragih, Winda A. M. Sarasati Windria Savira Ekawardhani Shabarni Gaffar Shabarni Gaffar -, Shabarni Gaffar Shabrinna, Hanif Siti Silvia Nur Shofa Shamantri Suharman, Khuznul Khatimah Sukamto, Charissa Otniel Supriyadi, Isma Yustifania Syafrizal Saragih Tati Herlina Usman, Hermin A. Veronica Oladitha Siagian Wijaya, Muhammad Palar Yasfira Aradella Yeni Wahyuni Hartati Yoga Windhu Wardhana

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