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All Journal AQUATIC SCIENCE & MANAGEMENT JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX Media Akuakultur
Pangemanan, Novie Pankie Lukas
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

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Optimisation Of Papain Enzyme Use As An Effort To Increase The Hatching Degree Of Mutiara Catfish (Clarias gariepinus) Eggs Simangunsong, Martua Pinondang; Sambali, M.Sc, Hariyani; Pangkey, Henneke; Mokolensang, Jeffrie Fredrik; Pangemanan, Novie Pankie Lukas; Sumilat, Deiske Adeliene
Jurnal Ilmiah Platax Vol. 13 No. 1 (2025): ISSUE JANUARY-JUNE 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i1.61097

Abstract

This study aims to analyze the effect of commercial papain enzyme with different concentrations and duration of soaking on increasing the degree of hatching of pearl catfish (Clarias gariepinus) eggs. The study used a Randomised Group Design (RAK) with variations in papain enzyme concentration (0, 2, 4, 6, and 8 ppm) and soaking duration (5, 10, and 15 minutes). The results showed that papain enzyme concentration and soaking duration significantly affected the degree of hatching (p < 0,001). The optimal concentration of papain enzyme was 6 ppm with a soaking duration of 10 minutes, resulting in the highest hatching rate of 70,52%±2,92. The lowest egg adhesion was produced at a concentration of 8 ppm with a duration of 15 minutes (32%±4,00), while the highest degree of fertilization was directed at a concentration of 6 ppm with a duration of 10 minutes (79%±3,61). The measurement results of water quality parameters are: temperature 25,6-26,4°C, pH 7,12-7,47, and dissolved oxygen 4,06-4,41 ppm. These results indicate that the papain enzyme effectively reduces egg adhesion and increases the hatching rate of pearl catfish eggs.  Keywords: mutiara catfish; papain enzyme; hatching rate; concentration; immersion duration.   Abstrak Penelitian ini bertujuan untuk menganalisis pengaruh enzim papain komersial dengan konsentrasi dan durasi perendaman yang berbeda terhadap peningkatan derajat penetasan telur ikan lele mutiara (Clarias gariepinus). Penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan variasi konsentrasi enzim papain (0, 2, 4, 6 dan 8 ppm) dan durasi perendaman (5, 10 dan 15 menit). Hasil penelitian menunjukkan bahwa konsentrasi enzim papain dan durasi perendaman berpengaruh signifikan (p <0,001) terhadap derajat penetasan. Konsentrasi optimal enzim papain adalah 6 ppm dengan durasi perendaman 10 menit, menghasilkan derajat penetasan tertinggi sebesar 70,52%±2,92. Daya rekat telur terendah dihasilkan pada konsentrasi 8 ppm dengan durasi 15 menit (32%±4,00), sedangkan derajat pembuahan tertinggi dihasilkan pada konsentrasi 6 ppm dengan durasi 10 menit (79%±3,61). Hasil pengukuran parameter kualitas air yaitu: suhu 25,6–26,4°C, pH 7,12–7,47 dan oksigen terlarut 4,06–4,41 ppm. Hasil ini menunjukkan bahwa enzim papain efektif mengurangi daya rekat telur dan meningkatkan derajat penetasan telur ikan lele mutiara.  Kata kunci: ikan lele mutiara; enzim papain; derajat penetasan; konsentrasi; durasi perendaman
KARAKTERISTIK MORFOMETRIK DAN POLA PERTUMBUHAN IKAN NILA (Oreochromis niloticus) YANG DIBERI PAKAN MIKROKAPSUL INKLUSI BERBEDA Fintarji, Rian; Pangkey, Henneke; Mokolensang, Jeffrie Fredrik; Sambali, Hariyani; Kalesaran, Ockstan Jurike; Pangemanan, Novie Pankie Lukas; Sumilat, Deiske Adeliene
Media Akuakultur Vol 20, No 1 (2025): Juni 2025
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.20.1.2025.35-44

Abstract

Produksi perikanan budidaya dapat ditingkatkan melalui penyediaan pakan yang sesuai dengan kebutuhan ikan, mencakup aspek jenis, ukuran, maupun jumlahnya. Pakan mikrokapsul adalah jenis pakan buatan berukuran sangat kecil (mikron) yang dirancang khusus untuk memenuhi kebutuhan nutrisi ikan pada tahap larva dan benih.Tujuan penelitian ini adalah untuk menganalisis karakteristik morfometrik dan pola pertumbuhan ikan yang diberi pakan mikrokapsul inklusi berbeda. Penelitian ini menggunakan 1.080 ekor benih ikan nila merah berukuran 1-3 cm pada wadah akuarium. Akuarium yang digunakan berukuran 60x40x40 cm yang kemudian diisi air sebanyak 72 L dan ditebar sebanyak 1 ekor L-1. Penelitian ini didesain dengan menggunakan rancangan acak lengkap dengan lima perlakuan dengan masing-masing tiga ulangan. Perlakuan satu yaitu pemberian pakan komersil (kontrol), perlakuan dua yaitu pemberian pakan mikrokapsul tepung Tubifex sp., perlakuan tiga yaitu pemberian pakan mikrokapsul tepung Maggot, perlakuan empat yaitu pemberian pakan mikrokapsul tepung Spirulina sp., dan perlakuan lima yaitu pemberian pakan mikrokapsul tepung Chlorella sp.. Hasil penelitian menunjukkan bahwa karakteristik morfometrik ikan nila berbeda pada setiap perlakuan. Hasil uji regresi linear sederhana terhadap parameter pola pertumbuhan ikan nila pada semua perlakuan bersifat alometrik negatif, yakni pertumbuhan panjang lebih cepat daripada pertumbuhan beratnya. Hasil pengukuran kualitas air pada semua perlakuan menunjukkan hasil yang sesuai dengan baku mutu untuk pemeliharaan ikan nila dengan kisaran nilai suhu berkisar 25,1-29,6 o C, oksigen terlarut berkisar 5,1-6,8 mg L-1, pH berkisar 7,42-8,14, dan amonia berkisar 0,01-0,17 mg L-1.Aquaculture production can be increased by providing feed that is appropriate to the need of fish, both in terms of type, size, and quantity. Microcapsule feed is a type of artificial feed with very small sizes (microns) specifically designed to fulfil the nutritional need at the larval and fry fish stages. This study aimed to analyze the morphometric characteristics and growth patterns of fed microcapsules with different inclusions. The study was conducted using 1.080 tilapia with size of 1-3 cm in aquariums. The aquarium used was 60x40x40 cm and was then filled with 72 L of water with a stocking density of 1 fish L-1. This study used a completely randomized design with five treatments and three replications each. Treatment one is the provision of commercial feed (control), treatment two is the provision of microcapsule feed with Tubifex sp flour, treatment three is the provision of microcapsule feed with Maggot flour, treatment four the provision of microcapsule feed with Spirulina sp flour, and treatment five is the provision of microcapsule feed with Chlorella sp flour. The results of the study showed that the morphometric characteristics of tilapia were different in each treatment. The results of the simple linear regression test on the parameters of tilapia growth patterns in all treatments were negative allometric, namely the growth in length was faster than the growth in weight. The results of water quality measurements in all treatments showed results that were in accordance with quality standards for tilapia with a temperature range of 25,1-29,6 oC, dissolved oxygen range of 5,1-6,8 mg L-1, pH range of 7,42-8,14, and ammonia range of 0,01-0,17 mg L-1.
The Effectiveness of Apu Wood Plants (Pistia stratiotes) in Reducing Detergents in Water Suwardy, Moch; Sambali, Hariyani; Rumampuk, Natalie Detty C.; Pangemanan, Novie Pankie Lukas; Sumilat, Deiske A.; Singkoh, Marina Flora Oktavine
Jurnal Ilmiah PLATAX Vol. 13 No. 2 (2025): ISSUE JULY-DECEMBER 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i2.65931

Abstract

This study aims to (1) evaluate the concentration of detergents in the water of the Dayanan River, (2) analyze the ability of Pistia stratiotes to reduce detergents, and (3) evaluate the effect of phytoremediation media on the physiological response of carp (Cyprinus carpio). The study used a Complete Random Design (RAL) with a plant contact time treatment of 3 and 6 days on two types of media (river water and well water + 10 mg/L detergent), as well as three replicas. Detergent analysis was carried out using the SNI 06-6989.51-2005 spectrophotometry method. The results showed that the highest concentration of detergent in the Dayanan River was found in the middle (0.0497 mg/L). Pistia stratiotes effectively reduced detergent significantly (p = 0.025) with optimal time on the third day. The fish response showed an increase in the frequency of operculum opening on the third day (p = 0.004) and no mortality was found for 48 hours. These results prove that Pistia stratiotes is effectively used as a natural biofilter to reduce the detergent content in waters. Keywords: detergent, Pistia stratiotes, phytoremediation, common carp (Cyprinus carpio), Dayanan River Abstrak Penelitian ini bertujuan untuk (1) mengevaluasi konsentrasi detergen dalam air Sungai Dayanan, (2) menganalisis kemampuan Pistia stratiotes dalam mereduksi detergen, dan (3) mengevaluasi efek media hasil fitoremediasi terhadap respon fisiologis ikan mas (Cyprinus carpio). Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan waktu kontak tanaman 3 dan 6 hari pada dua jenis media (air sungai dan air sumur + detergen 10 mg/L), serta tiga ulangan. Analisis detergen dilakukan menggunakan metode spektrofotometri SNI 06-6989.51-2005. Hasil penelitian menunjukkan bahwa konsentrasi detergen tertinggi di Sungai Dayanan terdapat pada bagian tengah (0,0497 mg/L). Pistia stratiotes efektif mereduksi detergen secara signifikan (p = 0,025) dengan waktu optimal pada hari ketiga. Respon ikan menunjukkan peningkatan frekuensi bukaan operkulum pada hari ketiga (p = 0,004) dan tidak ditemukan mortalitas selama 48 jam. Hasil ini membuktikan bahwa Pistia stratiotes efektif digunakan sebagai biofilter alami untuk menurunkan kandungan detergen di perairan. Kata kunci: detergen, Pistia stratiotes, fitoremediasi, ikan mas (Cyprinus carpio), Sungai Dayanan
Proximate composition of tilapia feed formulated with varying levels of moringa (Moringa oleifera) leaf flour Talunga, Junianto; Kreckhoff, Reni Lucia; Lintang, Rosita A. J.; Sambali, Hariyani; Pangemanan, Novie Pankie Lukas; Salaki, Christina Leta; Sumilat, Deiske Adeliene
AQUATIC SCIENCE & MANAGEMENT Vol. 13 No. 2 (2025): OCTOBER
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.v13i2.64291

Abstract

This study evaluated the effect of incorporating moringa leaf flour (Moringa oleifera) at varying concentrations into feed formulations on the proximate composition of tilapia (Oreochromis niloticus) feed. Five experimental treatments were tested, with moringa leaf flour inclusion levels of 0% (control), 10%, 20%, 30%, and 40%. A proximate analysis was conducted to determine moisture, ash, fat, protein, and crude fiber content. The results indicated that only the control feed (0%) and the feed containing 10% moringa leaf flour met the required protein standards for formulated feed, at 30% and 25%, respectively. Feeds with higher inclusion levels had protein content below the minimum threshold. The lowest crude fiber content (14.98%) was recorded in the 10% treatment, which was lower than that of the control. Ash, fat, and moisture contents remained relatively stable across all treatments and within acceptable ranges for fish feed. The formulation with 10% moringa leaf flour provided the optimal nutritional profile, delivering adequate protein (25%) and the lowest crude fiber (14.98%), thereby meeting nutritional requirements without compromising digestibility. Based on proximate analysis, moringa leaf flour shows potential as an efficient alternative feed ingredient for tilapia aquaculture.
The Potential of Moringa oleifera Leaves as a Natural Anti-Stress Ingredient in Fish Feed Based on Qualitative Phytochemical Screening Talunga, Junianto; Kreckhoff, Reni Lucia; Lintang, Rosita A. J.; Sambali, Hariyani; Pangemanan, Novie Pankie Lukas; Sumilat, Deiske Adeliene; Salaki, Christina Leta
JURNAL PESISIR DAN LAUT TROPIS Vol. 13 No. 3 (2025): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.13.3b.2025.65055

Abstract

This study aims to evaluate the potential of Moringa oleifera leaves as a natural anti-stress ingredient in fish feed through qualitative phytochemical screening. The extraction was conducted using maceration with 96% ethanol, followed by solvent evaporation using a rotary evaporator and phytochemical assays employing Dragendorff, Wagner, Mayer, AlCl₃, FeCl₃, concentrated H₂SO₄, and froth tests to identify major groups of bioactive metabolites. The results confirmed a very strong presence of alkaloids, along with positive reactions for flavonoids, tannins, saponins, phenolics, and triterpenoids, while steroids were not detected. This metabolite profile indicates antioxidant, immunomodulatory, antimicrobial, and anti-inflammatory activities that are relevant for reducing physiological stress in fish. These findings demonstrate that ethanol extracts of M. oleifera leaves possess practical potential as an anti-stress feed additive capable of enhancing fish resilience to biotic and abiotic stressors in intensive aquaculture. Practically, the use of M. oleifera leaves offers an accessible and economical natural ingredient for the development of functional aquafeed formulations. Keywords: antistress, aquaculture, moringa leaves, phytochemistry, bioactive compounds   Abstrak Penelitian ini bertujuan menilai potensi daun kelor (Moringa oleifera) sebagai bahan anti-stres alami dalam pakan ikan melalui skrining fitokimia kualitatif. Ekstraksi dilakukan menggunakan metode maserasi dengan pelarut etanol 96%, diikuti penguapan menggunakan rotary evaporator dan pengujian fitokimia menggunakan pereaksi Dragendorff, Wagner, Mayer, AlCl₃, FeCl₃, H₂SO₄ pekat, serta uji busa untuk mengidentifikasi golongan metabolit bioaktif. Hasil menunjukkan keberadaan alkaloid sangat kuat, serta flavonoid, tanin, saponin, fenolik, dan triterpenoid dengan reaksi positif, sementara steroid tidak terdeteksi. Profil metabolit tersebut mengindikasikan aktivitas antioksidan, imunomodulator, antimikroba, dan antiinflamasi yang relevan untuk menurunkan stres fisiologis pada ikan. Temuan ini menegaskan bahwa ekstrak etanol daun kelor memiliki potensi aplikatif sebagai aditif pakan antistres yang dapat meningkatkan ketahanan ikan terhadap stres biotik dan abiotik dalam budidaya intensif. Secara praktis, pemanfaatan daun kelor berpeluang mendukung formulasi pakan fungsional berbahan alami yang mudah diperoleh dan ekonomis bagi industri akuakultur.   Kata kunci: antistres, budidaya, daun kelor, fitokimia, senyawa bioaktif
Co-Authors Deiske Adeliene Sumilat, Deiske Adeliene Fintarji, Rian Henneke Pangkey Junianto Talunga, Junianto Kalesaran, Ockstan Jurike Kreckhoff, Reni Lucia Lintang, Rosita AJ Mokolensang, Jeffrie Fredrik Rumampuk, Natalie Detty C. Salaki, Christina Leta Sambali, Hariyani Sambali, M.Sc, Hariyani Simangunsong, Martua Pinondang Singkoh, Marina Flora Oktavine Suwardy, Moch