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All Journal HAYATI Journal of Biosciences AQUATIC SCIENCE & MANAGEMENT Jurnal Kesehatan Tambusai Jurnal Agroekoteknologi Terapan
Salaki, Christina Leta
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health

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Integrated DNA Barcoding and Morphometric Characterization of Palm Weevils (Rhynchophorus spp.) in North Sulawesi Balansa, Endrile Golmen; Salaki, Christina Leta; Tarore, Dantje; Mamahit, Juliet Merry Eva; Kolondam, Beivy Jonathan; Tallei, Trina Ekawati
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1260-1272

Abstract

Palm weevils (Rhynchophorus spp.) are significant pests of sago palms worldwide. Yet, the taxonomy and evolutionary lineage of these species in North Sulawesi remain unclear, likely due to geographic isolation driving genetic variation and species differentiation. This study aimed to investigate the genetic diversity and morphological differentiation of Rhynchophorus across distinct geographic regions in North Sulawesi using an integrative approach combining DNA barcoding and morphometric analysis. Morphometric traits from palm weevil specimens collected in Sangihe Island, Minahasa, and Bolaang Mongondow were measured and statistically analyzed using one-way ANOVA, MANOVA, principal component analysis (PCA), and linear discriminant analysis (LDA) to assess interpopulation morphological differences. For molecular identification, the cytochrome oxidase I (COI) gene was amplified and sequenced. Phylogenetic relationships were inferred using the maximum likelihood method, and a DNA barcode gap analysis was conducted to evaluate the separation between intra- and interspecific genetic distances. Morphometric analysis revealed significant size variations among the specimens, particularly in rostrum dimensions, with the EBBM (Bolaang Mongondow) group showing the highest values. COI-gene-based identification confirmed that all specimens were of R. vulneratus. However, phylogenetic analysis showed EBMin (Minahasa) and EBBM forming a distinct subgroup, while EBMan, EBSTS, EBTam, and EBSTU (all from Sangihe Island) clustered separately. Barcode gap analysis demonstrated a clear distinction between intra- and interspecific divergence, validating COI as a reliable marker for species delimitation. This study concludes that integrating morphometric and genetic analyses reveals geographic structuring within R. vulneratus, highlighting the effectiveness of combined methods for accurate identification and population differentiation.
ANALISIS HUBUNGAN FAKTOR RISIKO DAN DIAGNOSIS BERDASARKAN GAMBARAN HISTOPATOLOGI PADA PASIEN KANKER PAYUDARA DI RSUP PROF. DR. R. D. KANDOU MANADO Wijaya, Nur Anindhita Kurniawaty; Lampus, Harsali Fransiscus; Kepel, Billy Johnson; Bolang, Alexander Sam Leonard; Kaseke, Martha Marie; Salaki, Christina Leta
Jurnal Kesehatan Tambusai Vol. 5 No. 2 (2024): JUNI 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v5i2.30077

Abstract

Adapun tujuan dari penelitian ini adalah untuk menganalisis hubungan faktor risiko dan diagnosis berdasarkan gambaran histopatologi pada pasien kanker payudara di RSUP Prof. dr. R. D. Kandou Manado Tahun 2022-2023. Penelitian ini merupakan penelitian kuantitatif yang bersifat retrospektif analitik. Teknik pengambilan sampel adalah total sampling. Sampel penelitian sebanyak 375 pasien kanker payudara yang memiliki hasil histopatologi pada Electronic Medical Record (EMR). Teknik analisis statistik yang digunakan yaitu univariat, bivariat yaitu menggunakan Chi Square dan Fisher Exact Test serta multivariat yaitu regresi logistik berganda model prediksi.
Proximate composition of tilapia feed formulated with varying levels of moringa (Moringa oleifera) leaf flour Talunga, Junianto; Kreckhoff, Reni Lucia; Lintang, Rosita A. J.; Sambali, Hariyani; Pangemanan, Novie Pankie Lukas; Salaki, Christina Leta; Sumilat, Deiske Adeliene
AQUATIC SCIENCE & MANAGEMENT Vol. 13 No. 2 (2025): OCTOBER
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.v13i2.64291

Abstract

This study evaluated the effect of incorporating moringa leaf flour (Moringa oleifera) at varying concentrations into feed formulations on the proximate composition of tilapia (Oreochromis niloticus) feed. Five experimental treatments were tested, with moringa leaf flour inclusion levels of 0% (control), 10%, 20%, 30%, and 40%. A proximate analysis was conducted to determine moisture, ash, fat, protein, and crude fiber content. The results indicated that only the control feed (0%) and the feed containing 10% moringa leaf flour met the required protein standards for formulated feed, at 30% and 25%, respectively. Feeds with higher inclusion levels had protein content below the minimum threshold. The lowest crude fiber content (14.98%) was recorded in the 10% treatment, which was lower than that of the control. Ash, fat, and moisture contents remained relatively stable across all treatments and within acceptable ranges for fish feed. The formulation with 10% moringa leaf flour provided the optimal nutritional profile, delivering adequate protein (25%) and the lowest crude fiber (14.98%), thereby meeting nutritional requirements without compromising digestibility. Based on proximate analysis, moringa leaf flour shows potential as an efficient alternative feed ingredient for tilapia aquaculture.
Effectiveness of coconut water (Cocos nucifera) in feed on masculinization and growth factors of nile tilapia (Oreochromis niloticus) Sukmawati; Kalesaran, Ockstan J.; Warouw, Veibe; Mokolensang, Jeffrie F.; Paransa, Darus Sa'adah Johanis; Salaki, Christina Leta
AQUATIC SCIENCE & MANAGEMENT Vol. 13 No. 2 (2025): OCTOBER
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.v13i2.64394

Abstract

This study evaluated the effectiveness of coconut water (Cocos nucifera) applied as a spray onto feed for the masculinization and growth performance of Nile tilapia (Oreochromis niloticus). The 63-day experiment employed a completely randomized design (CRD) with five treatments of coconut water doses (0, 0.10, 0.15, 0.20, and 0.25 mL/g feed) and three replications. Measured parameters included male proportion, growth performance (absolute length and weight, specific growth rate), survival rate, and water quality. Results indicated that coconut water supplementation significantly influenced masculinization and growth. The optimal dose was 0.15 mL/g feed, which yielded the highest male proportion (63.65%)—a 13.65% increase over the control. At this dose, absolute length and weight reached 7.15 cm and 5.34 g, respectively, with a specific growth rate of 8.62%. In contrast, higher doses (0.20-0.25 mL/g) reduced both masculinization and growth performance, despite high survival rates (90-95%). Water quality parameters remained within optimal ranges throughout the trial. These findings suggest that coconut water is a viable, eco-friendly alternative to synthetic hormones for tilapia masculinization and can enhance growth when applied at the recommended dosage.
Application of Local Bacillus Thuringiensis Isolates as Biopesticides for Control of Spodoptera Frugiperda in Maize Salaki, Christina Leta; Makal, Henny V.; Paat, Frangky J.; Pamandungan, Yefta
Jurnal Agroekoteknologi Terapan (JAT) Vol. 7 No. 1 (2026): ISSUE JANUARY-JUNE 2026
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v7i1.66642

Abstract

The fall armyworm, Spodoptera frugiperda, is an invasive pest that threatens maize production and can increase dependence on synthetic insecticides, highlighting the need for locally effective biological control options. This study screened 24 local Bacillus thuringiensis isolates for insecticidal activity against third instar S. frugiperda using a maize leaf dip feeding bioassay. For initial screening, maize leaf sections were treated with 1.5 × 10⁷ spores mL⁻¹ and offered to larvae (30 larvae per isolate), with mortality recorded up to 72 h. Isolates producing at least 50 percent mortality at 72 h were advanced to pathogenicity testing. Selected isolates were evaluated at five concentrations (1.5 × 10³ to 1.5 × 10⁷ spores mL⁻¹) to estimate LC50 at 72 h, and at 1.5 × 10⁷ spores mL⁻¹ to estimate LT50; parameters were calculated by probit analysis and reported with 95 percent fiducial limits. Ten isolates met the screening criterion, and isolate ITH produced the highest mortality (93.3 percent) at 72 h. ITH also showed the greatest pathogenicity, with an LC50 of 7.5 × 10³ spores mL⁻¹ and an LT50 of 19.5 h, indicating high potency and rapid action relative to other candidates. The results demonstrate substantial variability among local Bt isolates and identify promising strains for further development. Future work should confirm isolate identity, characterize toxin profiles, optimize production and formulation, and validate efficacy and crop protection performance under semi field and field conditions for integration into maize integrated pest management. Keywords: Bacillus thuringiensis; biopesticide; fall armyworm; leaf dip bioassay; maize; Spodoptera frugiperda
Co-Authors Balansa, Endrile Golmen BEIVY JONATHAN KOLONDAM Bolang, Alexander Sam Leonard Dantje Tarore Deiske Adeliene Sumilat, Deiske Adeliene Frangky J. Paat Junianto Talunga, Junianto Kepel, Billy Johnson Kreckhoff, Reni Lucia Lampus, Harsali Fransiscus Lintang, Rosita AJ Makal, Henny V. Mamahit, Juliet Merry Eva Martha Marie Kaseke Mokolensang, Jeffrie F. Ockstan J. Kalesaran Pangemanan, Novie Pankie Lukas Paransa, Darus Sa'adah Johanis Sambali, Hariyani sukmawati TRINA EKAWATI TALLEI Veibe Warouw Wijaya, Nur Anindhita Kurniawaty Yefta Pamandungan