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All Journal Microbiology Indonesia Jurnal Tumbuhan Obat Indonesia Pharmaciana: Jurnal Kefarmasian Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Pharmascience Pharmasipha Media Farmasi PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Ilmu Kefarmasian Indonesia Jurnal Ilmiah Cendekia Eksakta Jurnal Ilmiah Wahana Pendidikan Jurnal Farmasi Sains dan Praktis Jurnal Kefarmasian Indonesia
Erika Indah Safitri
Universitas Wahid Hasyim
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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MIGRATION INHIBITION ACTIVITY BY METHANOL EXTRACT Hibiscus tiliaceus Linn. ON 4T1 BREAST CANCER Alviani, Dwi Lutvi; Safitri, Erika Indah; Hidayati, Devi Nisa
Jurnal Farmasi Sains dan Praktis Vol 10 No 2 (May-August 2024)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v10i2.9393

Abstract

The prevalence of breast cancer cases in Indonesia is increasing along with the ability of cancer cells to migrate or move from the primary tumor mass and form new colonies elsewhere. The migration of cancer cells has encouraged the develop anticancer drugs from natural ingredients. Waru leaves have been shown to have cytotoxic activity. This study aims to determine the inhibition activity of migration of breast cancer cells 4T1 from methanol extract of waru leaves. Waru leaves methanol extract was obtained using the maceration method. Cytotoxic test of methanol extract of waru leaves (MEWL) was the migration test used in the scratch wound healing method at concentrations 162.5, 325, and 650 μg/mL at 0, 18, 24, and 42 hours after treatment. Analysis of IC50 using linear regression, while large areas were analyzed using Image-J software. The percentage of data closure was analyzed statistically with the Anova Repeated Measure test. All concentrations of Methanol Extract of Waru Leaves had significant inhibition of cell migration (p<0,05) compared to control at each observation time at 0, 18, 24, and 42 hours after treatment. So, MEWL is able to inhibit migration in 4T1 cells
Antihyperpigmentation Effect of The Combination of Turmeric (Curcuma domestica Val.) and Bitter Melon Leaves (Momordica Charantia L.) Ethanol Extracts on Guinea Pig Skin Fitria, Risha Fillah; Anas, Yance; Safitri, Erika Indah
Jurnal Kefarmasian Indonesia VOLUME 8, NOMOR 1, FEBRUARI 2018
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v8i1.3724

Abstract

Turmeric (T) and Bitter Melon leaves (BM) extract has been proven in decreasing melanin contents in in vitro study, but their single extracts effects were lower than the positive control. A study confirmed the combination of plants extracts had melanogenic effect better than the positive control and their single extracts. This study aimed to investigate the anti-hyperpigmentation effect of the combination of T and BM extract on guinea pig skin and compared with the positive control group. This study used a post-test control design. Twenty-five guinea pigs were divided into 5 groups. The negative control group was given by dimethyl sulfoxide; the positive control group was given by a pharma cream that consists of hydroquinone, tretinoin, and fluocinolone acetonide. The combination of extracts was given to experimental groups with doses 500 μg/mL of T and 200 μg/mL of BM; 750 μg/mL of T and 400 μg/mL of BM; 1.000 μg/mL of T and 600 μg/mL of BM, respectively. All groups exposed to UV-B light in 2 minutes/day for 2 weeks. Each experimental group was given 1 ml combination extract once a day for 2 weeks and in the last step, skin biopsies were done. The histopathological examination was conducted by staining with Fontana-Masson and Nuclear Fast Red. The average percentage of melanin area were compared in all group and analyzed with the Kruskal Wallis test followed by Mann-Whitney test with 95% of confidence level. The result showed group-2 and 3 had the better effect than pharma cream.
Cytotoxic Activity of Eugenia polyantha Wight Young Leaves Purification Extract and Fraction on T47D Hidayati, Devi Nisa; Safitri, Erika Indah; Yumni, Gharsina Ghaisani; Salsabiila, Iqna; Rahayu, Putri
Jurnal Kefarmasian Indonesia VOLUME 13, NUMBER 2, AUGUST 2023
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v13i2.6607

Abstract

Breast cancer is a cancer that ranks the highest incidence in Indonesia. One of the plants that can be used for this treatment is a bay leaf (Eugenia polyantha Wight). Purification techniques can optimize the extraction of flavonoid compounds to create larger concentrations of these substances. Old bay leaves' ethyl acetate fraction has lethal effects on T47D cancer cells. In this work, the amounts of flavonoids and the cytotoxic activity of young bay leaves' pure extract and ethyl acetate fraction against T47D cells are to be determined. By using the maceration process, two phases of methanol extraction from young bay leaves were produced. In the first stage, ethyl acetate was used to partially purify the material, and in the second stage, n-hexane, ethyl acetate, and water. Cytotoxic tests of purified extract and ethyl acetate fraction of young bay leaves were carried out at 200; 350; 500; 750; and 1000 µg/mL. Cytotoxicity test using the MTT assay method. IC50 value analysis using linear regression. Determination of total flavonoid content using a UV-Visible spectrophotometer with a comparator compound, quercetin. The results showed that the purified extract of young bay leaves and the ethyl acetate fraction of methanol extract of young bay leaves had cytotoxic activity with IC50 values of 570.57 ± 30.72 µg/mL and  588.45 ± 6.90 µg/mL, respectively. Total flavonoid levels were 9.80 ± 0.05 mgQE/g and 5.99 ± 0.06 mgQE/g.The purified extract and ethyl acetate fraction on young bay leaf extract (Eugenia polyantha Wight) had cytotoxic activity on T47D cells and the highest levels of flavonoids in the purified extract.
ANTIBACTERIAL AND ANTIACNE ACTIVITY OF n-HEXANE FRACTION OF ALFALFA HERB (Medicago sativa L.) ETHANOL EXTRACT IN VITRO AND ON RABBIT (Oryctolagus cuniculus) Fithria, Risha Fillah; Safitri, Erika Indah; Khasanyzulaikhah, Kharista; Nurazisa, Nurazisa
CENDEKIA EKSAKTA Vol. 8 No. 1 (2023)
Publisher : Universitas Wahid Hasyim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31942/ce.v8i1.8257

Abstract

Propionibacterium acnes (P. acnes) can cause inflammation of the polysebaceous glands and lead to acne. Flavonoids in alfalfa herb are thought to have antibacterial and antiinflammatory properties. Flavonoids can be absorbed into n-hexane solvents. This study aims to determine antibacterial activity of n-hexane fraction of alfalfa herb ethanol extract (nHFAH) against P. acnes in vitro, to calculate the total flavonoid levels in the fraction, and to determine antiacne activity of the fraction on rabbit. This study is an experimental study with post-test control group design. Extraction of alfalfa herb was done by maceration method using 70% ethanol and fractionated with n-hexane. Antibacterial activity test was carried out using disk diffusion with 5 series of concentrations (15%, 20%, 25%, 30% dan 35%), positive control (clindamycin 2 µg/disk) and negative control (DMSO 100%). Diameters of the inhibition area were analyzed statistically using a Kruskal Wallis test followed by Mann Withney test with 95% of confidence level. Rabbit back skin was shaved on 5 different areas (3x3 cm2/area). Induced with 0.2 mL of P. acnes suspension (3x109 CFU/mL) intradermal. Acne formed (24 hours) on all areas, then were treated with T1 (clindamycin 2%), T2 (DMSO 100%), T3 (nHFAH 20%), T4 (nHFAH 25%), T5 (nHFAH 30%). Erythema diameter reduction on day 9 was analyzed using one-way anova, continued by post hoc tukey with 95% of confidence level. Total nHFAH flavonoid levels were tested by colorimetric method using UVVis spectrophotometer at λ 429.5 nm. The results showed that nHFAH has antibacterial activity against P. acnes with an average value of diameter of inhibition area for each concentration were 7.30; 7.49; 7.75; 7.92 and 8.24 mm. nHFAH exhibits antiacne activity with the percentage for each concentration were 67.01%; 77.84% and 85.84% respectively. Total nHFAH flavonoid levels were 6.09 mgQE/ gram.Keywords: Alfafa herb, n-Hexane fraction, Propionibacterium acnes.
Hubungan Usia, Pekerjaan dan Pendidikan Pasien Terhadap Tingkat Pengetahuan DAGUSIBU di Puskesmas Wilayah Lampung Tengah Sitepu, Debora Egyita; Primadiamanti, Annisa; Safitri, Erika Indah
Jurnal Ilmiah Wahana Pendidikan Vol 10 No 6 (2024): Jurnal Ilmiah Wahana Pendidikan
Publisher : Peneliti.net

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.10642605

Abstract

The DAGUSIBU movement aims to realize the rational use of medicine, people must know how to obtain, use, store and dispose of drugs properly. The purpose of this study is to determine the relationship between age, education and work of patients on DAGUSIBU knowledge at the Central Lampung Regional Health Center. This study used observational analytical methods with a cross-sectional approach. The sampling technique was carried out using the quota sampling method so that samples were obtained by all patients at the Seputih Besar Health Center, Suko Binangun Health Center, Seputih Mataram Health Center, Jati Datar Health Center and Rama Indra Health Center which met the inclusion criteria and exclusion criteria as many as 220 respondents with 44 respondents per puskesmas. The results of this study obtained age characteristics, namely early adulthood (26-35 years) as many as 62 respondents (28.18%), job characteristics, namely housewives as many as 69 respondents (31.36%), educational characteristics, namely secondary education as many as 191 respondents (86.82%). The result of p value.
Uji Efektivitas Antidepresan Mencit (Mus Musculus) Galur Swiss Menggunakan Ekstrak Etanol Kulit Jeruk Sunkist (Citrus sinensis (L.) Osbeck) Nofita, Nofita; Safitri, Erika Indah; Febriasti, Nisa A
Jurnal Ilmiah Wahana Pendidikan Vol 10 No 6 (2024): Jurnal Ilmiah Wahana Pendidikan
Publisher : Peneliti.net

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.10652546

Abstract

Depression is a disorder of a person's emotional or mental state which is characterized by persistent feelings of anxiety, sadness. Orange peel contains flavonoids, tannins, polyphenols and triterpenoids which are quite effective as antidepressants by providing a relaxing function to the body. This study aims to determine the antidepressant effectiveness of ethanol extract of sunkist orange peel (Citrus sinensis (L.) Osbeck). The method used for antidepressant test is FST (Forced Swim Test) and TST (Tail Suspension Test) and observed immobility time before and after treatment. The antidepressant test used 20 mice divided into 5 treatments, namely negative control (Na-CMC 0.5%), positive control (Amitriptyline 25 mg), doses of sunkist orange peel extract 200 mg/kgBW, 300 mg/kgBW and 400 mg/kgBW given orally. Immobility time data before and after treatment were statistically analyzed with Paired T-Test. The test results stated that all doses of ethanol extract of sunkist orange peel showed antidepressant effectiveness in mice that differed not statistically significant (p>0.05).
Cytotoxic Activity and Apoptosis by Extract and Ethyl Acetate Fraction of Hibiscus tiliaceus Linn in 4T1 Cell Line Hidayati, Devi Nisa; Safitri, Erika Indah; Surayya, Anis; Alviani, Dwi Lutvi; Putri, Masita Nur Amalia
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 1, FEBRUARY 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Waru leaves are proven to have cytotoxic activity. The activity of waru leaves needs to be developed in a molecular direction to see its apoptotic activity. This study aims to determine the cytotoxic activity of methanol extract (EWL) and ethyl acetate fraction fraction (EAFWL) of Hibiscus tiliaceus Linn as well as the induction of apoptosis in 4T1 cells. The methanol extract of hibiscus leaves was obtained by the maceration method, followed by a fractionation process with n-hexane, ethyl acetate, and water as solvents. Cytotoxic test of EWL was carried out at a concentration of 31.25; 62.5; 125; 250; 500 and 1000 μg/mL while EAFWL was at a concentration of 100; 200; 400; 600 and 100 μg/mL, using MTT assay. Analysis of IC50 values using linear regression. The apoptosis induction test at IC50 and ½ IC50 concentrations using flowcytometry. Data on the percentage of apoptosis were analyzed using One Way Anova statistical analysis. The results were EWL and EAFWL cytotoxic tests against 4T1 had IC50 values of 649 μg/mL and 746 μg/mL. EWL obtained a percentage of total apoptosis at a concentration of 324.5 μg/mL of 48.76%; 649 μg/mL was 67.06% and control cells were 36.16%. EAFWL percentage of total apoptosis at a concentration of 746 μg/mL of 30.8%; 373 μg/mL was 42.13% and control cells were 9.6%. So EWL and EAFWL can induce apoptosis of 4T1 cells.
Co-Authors Alviani, Dwi Lutvi Asri Melati Dewi AULIA EVERESTINA PUJAKA Ayu Shabrina Danang Yogo Wijaya Devi Nisa Hidayati Dewi Andini Kunti Mulangsri, Dewi Andini Kunti Dewi Listianingsih Dwi Ayu Mustikaningsih Dwi Lutvi Alviani Dwi Nadya Jayanthy Eko Wahyu Saputro Febriasti, Nisa A Fera Elia Fita Fitria, Risha Fillah Gharsina Ghaisani Yumni Ibrahim Arifin Intan Pratiwi Jeni Anggraini Khasanyzulaikhah, Kharista Laili Nailul Muna Masita Nur Amalia Putri Maulita Cut Nuria Misbahul Munir Nofita, Nofita Nurazisa, Nurazisa Primadiamanti, Annisa Putri, Masita Nur Amalia Rahayu, Putri Raka Galih Pradana risha fillah fithria Risha Fillah Fitria Salsabiila, Iqna Siska Maila Sari Sitepu, Debora Egyita Sumantri Sumantri Surayya, Anis Syahrul Iqbal Setiawan Yance Anas Yunita Ayu Hapsari