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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Masyarakat As-salam
Asryadin, Asryadin
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics Social Sciences Other

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PENCEGAHAN INFEKSI MENULAR SEKSUAL MELALUI PEMBERDAYAAN DAN DETEKSI DINI PADA REMAJA KARANG TARUNA Yuniati, Nilasari Indah; Khasanah, Nur Aini Hidayah; Rakhmawati, Aprilia; Wulansari, Keke Putri; Asryadin, Asryadin
JPMA - Jurnal Pengabdian Masyarakat As-Salam Vol. 5 No. 1 (2025): Jurnal Pengabdian Masyarakat As-Salam (JPMA)
Publisher : Asosiasi Dosen Perguruan Tinggi Islam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37249/jpma.v5i1.880

Abstract

Kelompok usia remaja tergolong rentan terhadap penularan infeksi menular seksual (IMS) akibat rendahnya literasi kesehatan reproduksi, perilaku seksual beresiko, dan keterbatasan akses terhadap layanan kesehatan yang ramah remaja. Program pengabdian kepada masyarakat (PKM) ini dirancang untuk meningkatkan pemahaman dan kesadaran remaja Karang Taruna di Kelurahan Karang Sentul melalui penyuluhan dan skrining IMS, khususnya HIV dan sifilis. Metode kegiatan meliputi penyuluhan edukatif dengan pendekatan interaktif. Pelaksanaan pre-test dan post-test dilakukan untuk mengukur kenaikan tingkat pengetahuan peserta tentang IMS. Selain itu, dilakukan skrining IMS menggunakan tes cepat (rapid diagnostic test). Sebanyak 20 peserta mengikuti kegiatan ini, terdiri dari 18 laki-laki dan 2 perempuan. Data pre-test memperlihatkan bahwa 60% peserta memiliki pemahaman kurang, yang kemudian meningkat menjadi 70% dengan kategori pemahaman baik setelah penyuluhan. Dari 18 peserta yang bersedia menjalani tes skrining, seluruhnya menunjukkan hasil negatif terhadap HIV dan sifilis. Kegiatan ini membuktikan bahwa edukasi yang tepat dan layanan skrining yang mudah diakses dapat meningkatkan kesadaran remaja terhadap IMS. Diperlukan kolaborasi berkelanjutan dengan lembaga terkait guna memperluas cakupan dan dampak kegiatan serupa.
Dengue Hemorrhagic Fever in Bima City: A Study Asryadin, Asryadin; Aqwam, Adhi; Khairunnisa, Rizka; Endang, Hetti Koes; Rosita, Rosita; Annas, Fahrul; Hasan, Hasan
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.4787

Abstract

Cases of Dengue Hemorrhagic Fever (DHF) in Bima City are of concern because of its widespread spread and the number of sufferers continues to grow. The Municipal Government of Bima, West Nusa Tenggara, has established an Extraordinary Event (KLB) status for DHF. This status was assigned as a response to the high increase in DHF cases over the past two months, namely January and February 2023. Although vector control efforts have been made, DHF cases continue to fluctuate and increase significantly in early 2023. Therefore, researchers have conducted a Quick Response Analysis of DHF cases in Bima City with the aim of providing information about DHF cases and analyzing this information, as well as developing recommendations for alternative solutions in preventing an increase in DHF cases in Bima City. Through the descriptive method, it was found that the incidence of DHF in Bima City tends to increase significantly at the beginning of 2023 compared to the previous two years, with 172 positive cases and 4 of them leading to death as of February 10, 2023. One of the things of concern based on the case analysis is the understanding and attitude of the community regarding environmental sanitation. Meanwhile, solutions that can be recommended are in the form of short, medium, and long term recommendations which can be used as a reference for consideration in establishing regulations to control dengue fever
Resistance To Antiretroviral Therapy In People With HIV Asryadin, Asryadin; Yuniati, Nilasari Indah; Panjenengan, Lalu Addien Faqih; Satriana, Baiq Trisna
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.5283

Abstract

Human Immunodeficiency Virus (HIV) is a virus that damages the immune system, and has RNA genetic material which is converted by the reverse transcriptase enzyme into DNA. This research aims to determine the existence of resistance by identifying mutations related to drug resistance in HIV-1, especially in the genes encoding the PR and RT enzymes which are the targets of ARVs. This research uses the observational method. Research data analysis was carried out in the form of descriptive data analysis of the output of each formula based on the results of HIV measurements. This study contains a series of reviews that focus on the topic and incidence and possibility of HIV drug resistance (ART) in PLHIV/PLWHA. The main and important thing in detecting the possibility of resistance to ARV therapy is by examining the genotype and phenotype. Standardization between laboratories for drug resistance studies through the use of various methods, especially to identify mixed bases that cause estimated resistance mutations. Additionally, sequencing of protease, RT, and/or integrase is used to identify the clinical significance of DRM. There are two genetic mechanisms of NRTI resistance, namely: (1) discriminative mutations that activate RT to differentiate between the dideoxy-NRTI chain terminator and the cell's own dNTP; and (2) unblocking mutations that facilitate phosphorylytic excision of NRTI-triphosphate from viral DNA. Blocking mutations are also referred to as thymidine analogue mutations (TAMs).   ARV resistance can be detected by examining the virus genotype which aims to determine the occurrence of mutations in one of the virus codons compared to ARV-sensitive wild type HIV-1 and by in vitro phenotyping, where this method takes quite a long time and is usually focused on finding a regimen new drug
Clean Water Quality Study in Bima City Asryadin, Asryadin; Aqwam, Adhi; Endang, Hetti Koes; Khairunnisa, Rizka; Nurhidayah, Jumratul; Hasan, Hasan; Rahadian, Muhammad; Annas, Fahrul; Muslimin, M. Ichwanul; Annas, Haerul; Israjunna, Israjunna
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.7236

Abstract

Changes in water quality are the main focus in water management because they can have a major impact on the ecosystem. Water quality includes physical, chemical and biological conditions. Coverage of clean water and drinking water inspection data in Bima City reached 97.41%, while the feasibility percentage based on clean water and drinking water quality tests only reached 51.13%. The low percentage of appropriate clean water quality requires special attention. Based on this, researchers studied problems related to water quality, supply and level of need for clean water in Bima City. This research is an analytical observational study, and sampling was carried out using a non-random purposive sampling technique, and analyzed descriptively. Based on the results of physical water quality tests on 31 samples, the results showed that the physical quality of water met the requirements, the chemical quality of water in all measured samples met the requirements for class 1 and 2 clean water quality standards for the parameters DO, COD, total chlorine, nitrate, nitrite, and six valence chrome, while the BOD, Phosphate and Iron values did not meet the requirements (1/31 samples). The biological quality test showed that 12 samples did not meet the requirements for total coliforms (38.7%) and 3 samples showed the presence of E. coli bacteria (9.7%). On the other hand, it was recorded that 95.8% of respondents used shallow drilled wells for clean water; pump wells for 12.5% of respondents; PDAM constituted 16.6% of respondents; shallow drilled wells and PDAM 16.6%; and those using shallow drilled wells and hand pump wells 12.5%. Meanwhile, 37.5% use clean, untreated water and 62.5% use refilled drinking water.
Development of a Rapid Diagnostic Method for Simultaneous Detection of Streptococcus viridans in Cases of Heart Disease Pestariati, Pestariati; Suhariyadi, Suhariyadi; Asryadin, Asryadin; Yuniati, Nilasari Indah
Jurnal Penelitian Pendidikan IPA Vol 11 No 11 (2025): November: In Progress
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i11.12730

Abstract

Heart disease is a leading cause of death worldwide, with a complex pathophysiology often involving interactions between genetic factors, the environment, and pathogenic microorganisms. Streptococcus viridans is a clinically significant pathogenic bacterium associated with infections of the cardiovascular system, including infective endocarditis, pericarditis, and other complications. However, timely and accurate diagnosis of this bacterial infection in cases of acute heart disease is often challenging, requiring rapid and sensitive diagnostic methods. Currently, diagnostic methods for detecting Streptococcus viridans in cases of acute heart disease tend to be time-consuming; therefore, developing a rapid diagnostic method that can detect both bacteria simultaneously is crucial. The aim of this study is to develop a rapid and sensitive diagnostic method for detecting the presence of Streptococcus viridans in samples from patients with heart disease. The method used is the identification of specific genes, the design of primer sequences, and the design of probes using specific 16s rRNA genes using bioinformatics techniques. Based on the research results obtained primer pair sequences are: oligonucleotide primer forward 5’-GCGACGATACATAGCCGAC-3’; primer reverse is 3’- CGAGCCAGTCTGAAAGC-5’, while the probe sequence is 5’-GACTGAGACACGGCCCAGACTC-3’. Primer and probe pair quality tests showed very good primer and probe quality for amplification with a 120 bp amplification product. Suggestions in the research are that it is necessary to continue with qPCR optimization to determine the melting temperature which is then carried out sensitivity tests of primer pair sequences and specific 16s rRNA Streptococcus viridans gene probes.
Co-Authors ., Pestariati Annas, Fahrul Annas, Haerul Aqwam, Adhi Endang, Hetti Koes Hasan Hasan Israjunna, Israjunna Khairunnisa, Rizka Khasanah, Nur Aini Hidayah Muslimin, M. Ichwanul Nurhidayah, Jumratul Panjenengan, Lalu Addien Faqih Rahadian, Muhammad Rakhmawati, Aprilia Rosita Rosita Satriana, Baiq Trisna Suhariyadi Suhariyadi Wulansari, Keke Putri Yuniati, Nilasari Indah