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All Journal International Journal of Public Health Science (IJPHS) Jurnal Teknologi Laboratorium Jurnal Penelitian Kesehatan Suara Forikes THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang Jaringan Laboratorium Medis Medica Arteriana (Med-Art) CENDEKIA : Jurnal Ilmu Pengetahuan Devotion: Journal of Research and Community Service IBTIDA : Jurnal Kajian Pendidikan Dasar HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Sewagati Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Pengabdian Kesehatan Beguai Jejama Frontiers in Community Service and Empowerment Health Dynamics
Suhariyadi Suhariyadi
Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Imunomodulator Ekstrak Etanol Daun Mimba (Azadirachta indica) terhadap Jumlah Sel Makrofag Peritoneal pada Mencit yang Diinduksi Vaksin BCG Abror, Yogi Khoirul; Woelansari, Evy Diah; Suhariyadi, Suhariyadi
Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.953 KB) | DOI: 10.29238/teknolabjournal.v7i1.110

Abstract

This research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p <0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients.
Korelasi Kadar High Sensitivity C-Reactive Protein (hs-CRP) Dan Kolesterol Low Density Lipoprotein (LDL) Pada Perokok Aktif Dengan Aktivitas Fisik Aliya Ayu Rizqiyah; Evy Diah Woelansari; Suhariyadi Suhariyadi
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14363

Abstract

Jombang Regency was the 10th most prevalent smoker in East Java in 2018 and experienced an increase in the prevalence of smokers by 0.1% from 2013. The high number of smokers raises a concern. Smoking may lead to an increase of hs-CRP and LDL cholesterol levels, where the increase in these two parameters is closely related to the formation of atherosclerotic plaques, as a cause of cardiovascular disease. Physical activity of each individual is something that needs to be known because it affects the levels of hs-CRP and LDL cholesterol. This study aims to analyze the correlation between hs-CRP and LDL cholesterol in active smokers with physical activity. This study used an observational analytic study with a cross-sectional design. This research was conducted in March-April 2022 with a total sample of 32 active smokers who work as pedicab drivers at Pondok Pesantren 'DU', Jombang Regency. The hs-CRP examination was carried out using the Latex Turbidimetric Assay method, while the LDL cholesterol examination was using the Homogeneous Enzymatic Colorimetric Assay method. Based on the statistical analysis of the Spearman correlation, the results obtained p>0.05 (p = 0.687, r = 0.074), which means that there is no correlation between levels of hs-CRP and LDL cholesterol in active smokers with physical activity.
PERBEDAAN INDEKS ERITROSIT SEBELUM DAN SESUDAH PEMBERIAN SUPLEMEN Spirulina platensis PADA PENDERITA DIABETES MELITUS TIPE 2 DI PUSKESMAS WARU SIDOARJO Salsabiil Rochman Alifiah; Suhariyadi Suhariyadi; Woelansari Evy Diah
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.735 KB)

Abstract

Diabetes mellitus type 2 is a metabolic disease that occurs due to abnormalities in insulin secretion, insulin action or both. One of the most common disorders in the body system is the red blood cell index consisting of MCV, MCH and MCHC. Spirulina platensis is a blue-green microalgae that contains phycocyanin so that it can increase hemoglobin levels, erythrocytes and blood profile in the bone marrow. The purpose of this study was to determine the difference before and after giving Spirulina platensis supplementation for 10 days on the erythrocyte index in patients with type 2 diabetes mellitus. This type of research was one group pretest-posttest. This research was conducted at the Waru Community Health Center, Sidoarjo Regency from December 2020 to May 2021. The results showed that the mean value of MCV decreased before: 80.19 fl and after: 80.1 fl. Meanwhile, the average value of MCH and MCHC increased, namely MCH before: 27.57 pg and after: 27.68 pg, then MCHC before: 34.33 g / dL and after: 34.51 g / dL. In the third statistical test, the significance value (p-value), namely the MCV (0.863), MCH (0.773) and MCHC (0.627) shows that (p> 0.05), it can be concluded that there is no significant difference before and after supplementation. Spirulina platensis on the erythrocyte index (MCV, MCH and MCHC) in people with type 2 diabetes mellitus.
PENGARUH PAPARAN Monosodium Glutamat (MSG) TERHADAP VIABILITAS SEL MONOSIT Fortuna Aliza Dewi; Suhariyadi Suhariyadi; Woelansari Evy Diah; Purwati Purwati
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The consumption rate of Monosodium Glutamate (MSG) in Indonesia has increased every year. Uncontrolled use of MSG in Indonesia for a long period of time can cause toxic effects on the body. The free glutamate content produced by MSG can affect the work of the immune system, especially in the innate immune system and cause oxidative stress. To determine the effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells. This study is a laboratory experimental in vitro with a post test only control group design. A total of 10cc of peripheral venous blood was isolated using the ficoll gradient centrifugation method. The results of monocyte cell isolates were exposed to Monosodium Glutamate (MSG) according to groups. Group I: negative control, group II: monocyte cells + MSG 3%, group III: monocyte cells + MSG 6%, group IV: monocyte cells + MSG 9%. Subsequently incubated for 24 hours at 37 °C in 5% CO2. Then the viability test was carried out using trypan blue staining. Monocyte cell viability calculations were carried out under an inverted microscope with a magnification of 400x per 100 cells. The data obtained were analyzed statistically using the one-way Anova test followed by the LSD test. The average viability in each group was obtained as follows, monocyte cell viability in the control group was 63%, group II was 47%, group III was 45% and group IV was 35%. There is an effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells with the most significant effect being the 9% MSG concentration with an average viability of 35%.
Pemeriksaan Status Gizi dan Kadar Asam Urat Lansia di Posyandu Abiyoso Polkesbaya Evy Diah Woelansari; Juliana Christyaningsih; Suhariyadi Suhariyadi; Lully Hanni Endarini; Museyaroh Museyaroh; Diah Titik Mutiarawati; Wisnu Istanto; Suliati Suliati; Anik Handayati; Sri Sulami Endah Astuti; Pestariati Pestariati; Retno Sasongkowati; Mamik Mamik; Sri Utami; Minarti Minarti; Luthfi Rusyadi; Liliek Soetjiatie
Jurnal Pengabdian Kesehatan Beguai Jejama Vol 4, No 2 (2023): Jurnal Pengabdian Kesehatan Beguai Jejama Volume 4 Nomor 2 Agustus 2023
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jpk.v4i2.13417

Abstract

Proses penuaan dapat memengaruhi kesehatan fisik lansia dan meningkatkan risiko berbagai gangguan kesehatan, termasuk hipertensi dan asam urat. Kegiatan ini berlangsung pada tanggal 14 Juni 2023 bertempat di Balai RW 2 Kelurahan Kertajaya, Surabaya dan dihadiri 59 lansia yang berdomisili di RW 1 dan RW 2 Kelurahan Kertajaya Kecamatan Gubeng Surabaya. Jenis kegiatan yang dilaksanakan adalah pengukuran status gizi, asam urat dan senam hipertensi. Hasil pemeriksaan mengungkapkan 57,6% lansia memiliki kadar asam urat yang tinggi dan mayoritas memiliki kelebihan berat badan, sehingga lansia perlu mengendalikan tekanan darah dengan pengobatan yang diresepkan oleh dokter, perubahan gaya hidup seperti diet rendah garam, olahraga teratur, dan menghindari stres.
The Effectiveness of Moringa Leaves in Controlling Blood Sugar Levels in Patients With Type 2 Diabetes Mellitus in Prolanis, Sampang District, Madura Anik Handayati; Pestariati Pestariati; Suhariyadi Suhariyadi
Frontiers in Community Service and Empowerment Vol. 1 No. 2 (2022): June
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v1i2.6

Abstract

Diabetes mellitus (DM) is a disease that leads to an increase in blood glucose levels, which in turn can lead to chronic diseases. The potential group to be socialized is the Prolanis group at Sreseh Health Centre, Sampang Madura. Moringa is a tree that grows very commonly in the Madura region, including in Sreseh sub-district. Moringa contains active ingredients that can lower blood glucose levels. People do not avail of the benefits of Moringa leaves  (Moringa aleivera), which are widely available in the region. The purpose of this community service activity is so that the community can be more optimal in controlling blood sugar levels, especially the use of Moringa leaf extract in lowering blood sugar levels. The method of conducting the activities is a group-based method that is carried out comprehensively, namely conducting regular and continuous counselling and consultations for Prolanis members. The activities of the community service teams are carried out in a measurable way and a monitoring and evaluation process is carried out to measure the achievement of the objectives and results. The results of the PKM activities have been identified: Community service activities (PKM) that have been well implemented, starting with survey and coordination activities, initial assessment activities, PKM implementation activities, and evaluation of the results achieved. The community, in this case, the Prolanis members with DM type 2, participated with great enthusiasm in each meeting/phase of the activities. The evaluation of the results of the activities showed good results: The number of subjects with type 2 diabetes whose blood glucose levels were uncontrolled decreased by 61.54%.
Correlation Of Neutrophil Lymphocyte Ratio (NLR) Value With Incidence Of Tuberculosis Disease In Patients With Diabetes Mellitus Istiqomah Istiqomah; Suhariyadi Suhariyadi; Evy Diah Woelansari
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.332

Abstract

Diabetes mellitus is a non-communicable disease (PTM) that is chronic and can weaken the immune system so that sufferers are 3 times more likely to suffer from pulmonary TB. In chronic infection there is continuous inflammation which is characterized by the presence of neutrophils themselves and will be followed by low lymphocyte values due to immune system pathways that do not work perfectly. The purpose of this study was to determine the correlation between the Neutrophil Lymphocyte Ratio (NLR) and the incidence of Tuberculosis in Diabetes Mellitus patients at the Jorongan Health Center, Probolinggo Regency. This research is a correlative analytic study with a cross sectional approach. A total of 30 samples of diabetes mellitus patients at the Jorongan Health Center from January to April 2023. The NLR examination method was with a hematology analyzer and tuberculosis patients were examined using Molecular Rapid Test. The statistical test uses Spearman's nonparametric test. The results showed that there was a correlation between NLR values and the incidence of tuberculosis in diabetes mellitus patients at the Jorongan Health Center, p = 0.003 and r = 0.521. Further research needs to be carried out involving larger samples and other laboratory tests related to the immune system in patients with diabetes mellitus.
Upaya Peningkatan Keaktifan Belajar Siswa Kelas 4 Tema 8 Menggunakan Model Student Team Achievement Division Suhariyadi, Suhariyadi; Eko, Sugeng; Suyoto, Suyoto
IBTIDA-Jurnal Kajian Pendidikan Dasar Vol 3 No 1 (2023)
Publisher : Program Studi Pendidikan Guru Madrasah Ibtidaiyyah (PGMI) IAINU Kebeumen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33507/ibtida.v3i1.1092

Abstract

This study aims to determine whether the STAD model can increase student activeness. This study used Classroom Action Research (PTK) which consisted of two cycles. Each cycle consists of two meetings. The subjects in the study were grade IV students of SDN Karanggedang totaling 11 students consisting of 9 boys and 2 girls. The data collection methods used were interviews, observation and documentation. STAD (Student Team Achievement Division) model". with stages 1) Conveying goals and motivation, 2) Group division, 3) Learning activities in teams, 4) Individual and group rewards, 5) Evaluation and conclusion. The application of the STAD (student team achievement division) model is very suitable to be applied in elementary schools. Based on observations about student activeness using the STAD (student team achievement division) model in the pre-cycle, the average percentage was 48.5%. Cycle I get a percentage of 57.9% and cycle II gets a percentage of 81.75%, so the STAD (student team achievement division) model is very well used to increase student activeness thus the research hypothesis is accepted. Based on observations from the implementation of cycle I learning gets an average percentage of 72.9%. In cycle II got an average score of 90.5%.
PERBEDAAN INDEKS ERITROSIT SEBELUM DAN SESUDAH PEMBERIAN SUPLEMEN Spirulina platensis PADA PENDERITA DIABETES MELITUS TIPE 2 DI PUSKESMAS WARU SIDOARJO Alifiah, Salsabiil Rochman; Suhariyadi, Suhariyadi; Evy Diah , Woelansari
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes mellitus type 2 is a metabolic disease that occurs due to abnormalities in insulin secretion, insulin action or both. One of the most common disorders in the body system is the red blood cell index consisting of MCV, MCH and MCHC. Spirulina platensis is a blue-green microalgae that contains phycocyanin so that it can increase hemoglobin levels, erythrocytes and blood profile in the bone marrow. The purpose of this study was to determine the difference before and after giving Spirulina platensis supplementation for 10 days on the erythrocyte index in patients with type 2 diabetes mellitus. This type of research was one group pretest-posttest. This research was conducted at the Waru Community Health Center, Sidoarjo Regency from December 2020 to May 2021. The results showed that the mean value of MCV decreased before: 80.19 fl and after: 80.1 fl. Meanwhile, the average value of MCH and MCHC increased, namely MCH before: 27.57 pg and after: 27.68 pg, then MCHC before: 34.33 g / dL and after: 34.51 g / dL. In the third statistical test, the significance value (p-value), namely the MCV (0.863), MCH (0.773) and MCHC (0.627) shows that (p> 0.05), it can be concluded that there is no significant difference before and after supplementation. Spirulina platensis on the erythrocyte index (MCV, MCH and MCHC) in people with type 2 diabetes mellitus.
The effectiveness of using ID broth in identifying the outer membrane protein of Salmonella typhi Pestariati, Pestariati; Suhariyadi, Suhariyadi
International Journal of Public Health Science (IJPHS) Vol 14, No 1: March 2025
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v14i1.23912

Abstract

Typhoid fever is an infection that affects the digestive system. It spreads through contaminated food and drinks due to the Salmonella bacteria. One way to develop immunity against Salmonella typhi is by using outer membrane protein (OMP), which activates the cellular immune system. This research aimed to determine the effectiveness of using ID Broth to identify OMP Salmonella typhi. The study was conducted experimentally at the Institute of Tropical Disease from April 2023 to May 2023. For the study, we obtained three samples of Salmonella typhi isolated from East Java, and each sample was replicated three times. We isolated the bacteria and extracted the OMP to measure its levels and perform electrophoresis with SDS-PAGE. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a standard protein analysis method. To address sample loading challenges due to stacking gel transparency, an acidic dye was added to improve visibility without affecting gel performance. In this study nutrient agar from MacConkey medium and ID broth were used as variables. We cultured Salmonella typhi and extracted OMP using the sonication technique. We measured protein levels through the nanodrop method. Salmonella typhi from ID broth produced higher protein levels than Salmonella typhi cultured from MacConkey Medium. It affected the identification of OMP using SDS-PAGE. Lower protein levels lead to fewer protein molecules in the same band zone, causing reduced visibility and readability of the protein bands. The ID broth stabilizes the bacteria's condition before being grown on nutrient agar media.
Co-Authors ., Pestariati Abror, Yogi Khoirul Alifiah, Salsabiil Rochman Aliya Ayu Rizqiyah Andreas Putro Ragil Santoso, Andreas Putro Ragil Anggraini, Anita Dwi Ariyani, Ahna Fitri Arta, Shela Yuni Asryadin Asryadin Cheppy Juwita Ayuningrum Diah Titik Mutiarawati Eko, Sugeng Endah Astuti, Sri Sulami Endarini, Lully Hanni Evy Diah Woelansari Fortuna Aliza Dewi Handayati, Anik Istanto, Wisnu istiqomah istiqomah Istiqomah Istiqomah Juliana Christyaningsih Kartika Rahayuningsih, Christ Krihariyani, Dwi Kumahartati, Intan Liliek Soetjiatie Luthfi Rusyadi Mamik Mamik Mamik Mamik Minarti Minarti Minarti Minarti Museyaroh, Museyaroh Mutiarawati, Diah Titik Nilasari Indah Yuniati Niswata, Navila Arfianti Novikasari, Caturizkyana Nur Alizah Octarina, Nofita Purwati Purwati Rahayuningsih, Christ. Kartika Rahma Yuliana, Putri Retno Sasongkowati Rizqiyah, Aliya Ayu Salsabiil Rochman Alifiah Sasongkowati, Retno Siti Helmyati Sri Utami Suliati Suliati Suliati Suliati Syamsul Arifin Uswatun Hasanah Widiastutik, Dyah Wisnu Istanto