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<![CDATA[Imunomodulator Ekstrak Etanol Daun Mimba (Azadirachta indica) terhadap Jumlah Sel Makrofag Peritoneal pada Mencit yang Diinduksi Vaksin BCG ]]>
<![CDATA[Abror, Yogi Khoirul]]>;
<![CDATA[Woelansari, Evy Diah]]>;
<![CDATA[Suhariyadi, Suhariyadi]]>
<![CDATA[ Jurnal Teknologi Laboratorium Vol 7 No 1 (2018): 2018 (1) ]]>
Publisher : <![CDATA[POLTEKKES KEMENKES YOGYAKARTA ]]>
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DOI: 10.29238/teknolabjournal.v7i1.110
<![CDATA[This research was conducted to determine the immunomodulatory effect of ethanol extract of neem leaves (Azadirachta indica) to the number of peritoneal macrophages in mice wich induced by BCG vaccine. Bacillus Calmette-Guerin (BCG) vaccine contained an attenuated Mycobacterium bovis. Mycobacterium bovis belongs to the Mycobacterium Tuberculosis Complex (MTC) group that has a similar phenotype characteristic with Mycobacterium tuberculosis and similar clinical manifestations of tuberculosis.The type of the research that used in this study is laboratory experimental research with Post Test Design Design Only Control Group Design. The research was conducted at the Faculty of Veterinary Medicine of Airlangga University in July 2017 using 25 male mice divided into five groups. The dosage of ethanol extract of the neem leaves given was 200 mg / Kg BW with variation for two days, four days, and six days are given.In the result of statistical data analysis using Kruskal-walis test, it is known that the significance value p = 0,03 (p <0,05), that means immunomodulatory of ethanol extract of neem leaves (Azadirachta indica) give an effect to peritoneal macrophage cell number in mice wich induced by BCG vaccine, so that neem leaves ethanol extract can be applied to tuberculosis patients.]]>
<![CDATA[ASSOCIATION BETWEEN TRIGLYCERIDE SERUM LEVELS AND GLOMERULAR FILTRATION RATE (eGFR) IN PATIENTS WITH CHRONIC RENAL FAILURE AT JEMURSARI ISLAMIC HOSPITAL SURABAYA, INDONESIA ]]>
<![CDATA[Uswatun Hasanah]]>;
<![CDATA[Suhariyadi]]>;
<![CDATA[Andreas Putro Ragil Santoso]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v2i2.1668
<![CDATA[Chronic Renal Failure (CRF) is a progressive and irreversible decrease in kidney function. One risk factor that affects the progression of CRF is dyslipidemia due to abnormalities of lipid metabolisms. Dyslipidemia is characterized by the increase level of total cholesterol, LDL cholesterol, triglycerides, and a decrease in HDL cholesterol level. eGFR is a parameter for assessing the excretion function, by calculating the amount of filtrate produced by the renal glomerulus. This study aims to determine the relationship between serum triglyceride levels with eGFR in patient suffering from CRF at Jemursari Islamic Hospital Surabaya. This type of research is descriptive experimental with cross–sectional design. The study population was the patients with CRF. The primary data in this study was the results of examination of serum triglyceride levels in patients with CRF. The secondary data (serum creatinine levels, body weight, age, and sex) was obtained from medical records of CRF patients in February 2019. As much as 12% of patients were in the 40–50 years old category, 36% of patients were 51–60 years old, 44% of patients were 61–70 years old, and 8% of patients were 71–80 years old. 72% of patients were male. The mean triglyceride level was 146.68 mg/dL and the average eGFR was 19.86 mL/min/1.73m2. The relationships between triglyceride levels and eGFR was carried out by the Spearman–rho test where r value was –0.442 and value was 0.027. It was concluded that there was a significant negative correlation between serum triglyceride levels and eGFR in patients with CRF.]]>
<![CDATA[Korelasi Kadar High Sensitivity C-Reactive Protein (hs-CRP) Dan Kolesterol Low Density Lipoprotein (LDL) Pada Perokok Aktif Dengan Aktivitas Fisik ]]>
<![CDATA[Aliya Ayu Rizqiyah]]>;
<![CDATA[Evy Diah Woelansari]]>;
<![CDATA[Suhariyadi Suhariyadi]]>
<![CDATA[ THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surabaya ]]>
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DOI: 10.30651/jmlt.v6i1.14363
<![CDATA[Jombang Regency was the 10th most prevalent smoker in East Java in 2018 and experienced an increase in the prevalence of smokers by 0.1% from 2013. The high number of smokers raises a concern. Smoking may lead to an increase of hs-CRP and LDL cholesterol levels, where the increase in these two parameters is closely related to the formation of atherosclerotic plaques, as a cause of cardiovascular disease. Physical activity of each individual is something that needs to be known because it affects the levels of hs-CRP and LDL cholesterol. This study aims to analyze the correlation between hs-CRP and LDL cholesterol in active smokers with physical activity. This study used an observational analytic study with a cross-sectional design. This research was conducted in March-April 2022 with a total sample of 32 active smokers who work as pedicab drivers at Pondok Pesantren 'DU', Jombang Regency. The hs-CRP examination was carried out using the Latex Turbidimetric Assay method, while the LDL cholesterol examination was using the Homogeneous Enzymatic Colorimetric Assay method. Based on the statistical analysis of the Spearman correlation, the results obtained p>0.05 (p = 0.687, r = 0.074), which means that there is no correlation between levels of hs-CRP and LDL cholesterol in active smokers with physical activity.]]>
<![CDATA[Correlation Of Neutrophil Lymphocyte Ratio (NLR) Value With Incidence Of Tuberculosis Disease In Patients With Diabetes Mellitus ]]>
<![CDATA[Istiqomah Istiqomah]]>;
<![CDATA[Suhariyadi Suhariyadi]]>;
<![CDATA[Evy Diah Woelansari]]>
<![CDATA[ Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS) ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Mataram ]]>
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DOI: 10.32807/jambs.v11i1.332
<![CDATA[Diabetes mellitus is a non-communicable disease (PTM) that is chronic and can weaken the immune system so that sufferers are 3 times more likely to suffer from pulmonary TB. In chronic infection there is continuous inflammation which is characterized by the presence of neutrophils themselves and will be followed by low lymphocyte values due to immune system pathways that do not work perfectly. The purpose of this study was to determine the correlation between the Neutrophil Lymphocyte Ratio (NLR) and the incidence of Tuberculosis in Diabetes Mellitus patients at the Jorongan Health Center, Probolinggo Regency. This research is a correlative analytic study with a cross sectional approach. A total of 30 samples of diabetes mellitus patients at the Jorongan Health Center from January to April 2023. The NLR examination method was with a hematology analyzer and tuberculosis patients were examined using Molecular Rapid Test. The statistical test uses Spearman's nonparametric test. The results showed that there was a correlation between NLR values and the incidence of tuberculosis in diabetes mellitus patients at the Jorongan Health Center, p = 0.003 and r = 0.521. Further research needs to be carried out involving larger samples and other laboratory tests related to the immune system in patients with diabetes mellitus.]]>
<![CDATA[Upaya Peningkatan Keaktifan Belajar Siswa Kelas 4 Tema 8 Menggunakan Model Student Team Achievement Division ]]>
<![CDATA[Suhariyadi, Suhariyadi]]>;
<![CDATA[Eko, Sugeng]]>;
<![CDATA[Suyoto, Suyoto]]>
<![CDATA[ IBTIDA-Jurnal Kajian Pendidikan Dasar Vol 3 No 1 (2023) ]]>
Publisher : <![CDATA[Program Studi Pendidikan Guru Madrasah Ibtidaiyyah (PGMI) IAINU Kebeumen ]]>
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DOI: 10.33507/ibtida.v3i1.1092
<![CDATA[This study aims to determine whether the STAD model can increase student activeness. This study used Classroom Action Research (PTK) which consisted of two cycles. Each cycle consists of two meetings. The subjects in the study were grade IV students of SDN Karanggedang totaling 11 students consisting of 9 boys and 2 girls. The data collection methods used were interviews, observation and documentation. STAD (Student Team Achievement Division) model". with stages 1) Conveying goals and motivation, 2) Group division, 3) Learning activities in teams, 4) Individual and group rewards, 5) Evaluation and conclusion. The application of the STAD (student team achievement division) model is very suitable to be applied in elementary schools. Based on observations about student activeness using the STAD (student team achievement division) model in the pre-cycle, the average percentage was 48.5%. Cycle I get a percentage of 57.9% and cycle II gets a percentage of 81.75%, so the STAD (student team achievement division) model is very well used to increase student activeness thus the research hypothesis is accepted. Based on observations from the implementation of cycle I learning gets an average percentage of 72.9%. In cycle II got an average score of 90.5%.]]>
<![CDATA[PERBEDAAN INDEKS ERITROSIT SEBELUM DAN SESUDAH PEMBERIAN SUPLEMEN Spirulina platensis PADA PENDERITA DIABETES MELITUS TIPE 2 DI PUSKESMAS WARU SIDOARJO ]]>
<![CDATA[Alifiah, Salsabiil Rochman]]>;
<![CDATA[Suhariyadi, Suhariyadi]]>;
<![CDATA[Evy Diah , Woelansari]]>
<![CDATA[ Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains ]]>
Publisher : <![CDATA[Politeknik Kesehatan Kementerian Kesehatan Surabaya ]]>
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<![CDATA[Diabetes mellitus type 2 is a metabolic disease that occurs due to abnormalities in insulin secretion, insulin action or both. One of the most common disorders in the body system is the red blood cell index consisting of MCV, MCH and MCHC. Spirulina platensis is a blue-green microalgae that contains phycocyanin so that it can increase hemoglobin levels, erythrocytes and blood profile in the bone marrow. The purpose of this study was to determine the difference before and after giving Spirulina platensis supplementation for 10 days on the erythrocyte index in patients with type 2 diabetes mellitus. This type of research was one group pretest-posttest. This research was conducted at the Waru Community Health Center, Sidoarjo Regency from December 2020 to May 2021. The results showed that the mean value of MCV decreased before: 80.19 fl and after: 80.1 fl. Meanwhile, the average value of MCH and MCHC increased, namely MCH before: 27.57 pg and after: 27.68 pg, then MCHC before: 34.33 g / dL and after: 34.51 g / dL. In the third statistical test, the significance value (p-value), namely the MCV (0.863), MCH (0.773) and MCHC (0.627) shows that (p> 0.05), it can be concluded that there is no significant difference before and after supplementation. Spirulina platensis on the erythrocyte index (MCV, MCH and MCHC) in people with type 2 diabetes mellitus.]]>
<![CDATA[The effectiveness of using ID broth in identifying the outer membrane protein of Salmonella typhi ]]>
<![CDATA[Pestariati, Pestariati]]>;
<![CDATA[Suhariyadi, Suhariyadi]]>
<![CDATA[ International Journal of Public Health Science (IJPHS) Vol 14, No 1: March 2025 ]]>
Publisher : <![CDATA[Intelektual Pustaka Media Utama ]]>
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DOI: 10.11591/ijphs.v14i1.23912
<![CDATA[Typhoid fever is an infection that affects the digestive system. It spreads through contaminated food and drinks due to the Salmonella bacteria. One way to develop immunity against Salmonella typhi is by using outer membrane protein (OMP), which activates the cellular immune system. This research aimed to determine the effectiveness of using ID Broth to identify OMP Salmonella typhi. The study was conducted experimentally at the Institute of Tropical Disease from April 2023 to May 2023. For the study, we obtained three samples of Salmonella typhi isolated from East Java, and each sample was replicated three times. We isolated the bacteria and extracted the OMP to measure its levels and perform electrophoresis with SDS-PAGE. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a standard protein analysis method. To address sample loading challenges due to stacking gel transparency, an acidic dye was added to improve visibility without affecting gel performance. In this study nutrient agar from MacConkey medium and ID broth were used as variables. We cultured Salmonella typhi and extracted OMP using the sonication technique. We measured protein levels through the nanodrop method. Salmonella typhi from ID broth produced higher protein levels than Salmonella typhi cultured from MacConkey Medium. It affected the identification of OMP using SDS-PAGE. Lower protein levels lead to fewer protein molecules in the same band zone, causing reduced visibility and readability of the protein bands. The ID broth stabilizes the bacteria's condition before being grown on nutrient agar media.]]>
<![CDATA[Skrining Antibodi IgM & IgG Toksoplasmolisis pada Ibu Hamil di Puskesmas Kabupaten Sampang ]]>
<![CDATA[Novikasari, Caturizkyana]]>;
<![CDATA[Suhariyadi, Suhariyadi]]>;
<![CDATA[Woelansari, Evy Diah]]>;
<![CDATA[Museyaroh, Museyaroh]]>
<![CDATA[ JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 18 No 2 (2023): JPP (Jurnal Kesehatan Poltekkes Palembang) ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Palembang ]]>
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DOI: 10.36086/jpp.v18i2.1839
<![CDATA[Latar Belakang: Toksoplasmosis adalah kelainan yang ditimbulkan akibat terjadinya infeksi Toxoplasma gondii. Infeksi toksoplasmosis dapat terjadi secara vertikal, dari ibu pada calon bayi melalui plasenta, sehingga dapat menyebabkan keguguran, kecacatan ataupun lahir mati. Tujuan penelitian ini adalah untuk mengetahui adanya antibodi IgM & IgG toksoplasmosis pada ibu hamil di Puskesmas Torjun Kabupaten Sampang. Metode: Penelitian ini menggunakan metode immunokromatografi kualitatif dengan hasil positif/negatif. Alat pemeriksaan yang digunakan yaitu cassette rapid test Dalf Toxo IgM/IgG. Sampel pemeriksaan yang digunakan berjumlah 25 serum ibu hamil yang melakukan periksa rutin di Puskesmas Torjun. Hasil: Pemeriksaan yang telah dilakukan terhadap 25 sampel darah ibu hamil di Puskesmas Torjun diperoleh sebanyak 25 (100%) ibu hamil negatif IgM toksoplasma. Ibu hamil dengan antibodi IgG toksoplasma positif sebanyak 5 (20%). Ibu hamil dengan antibodi IgG toksoplasma negatif diperoleh sebanyak 20 (80%). Kesimpulan: Berdasarkan hasil penelitian, ibu hamil di Puskesmas Torjun Kabupaten Sampang yang memiliki antibodi IgG toksoplasma positif sebesar 20% tanpa gejala selama kehamilan.]]>
<![CDATA[The Relationship Between TSH and Indirect Bilirubin Levels in Neonates Suspected of Having Jaundice ]]>
<![CDATA[Alizah, Nur]]>;
<![CDATA[Handayati, Anik]]>;
<![CDATA[Museyaroh, Museyaroh]]>;
<![CDATA[Suhariyadi, Suhariyadi]]>
<![CDATA[ Health Dynamics Vol 1, No 10 (2024): October 2024 ]]>
Publisher : <![CDATA[Knowledge Dynamics ]]>
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DOI: 10.33846/hd11006
<![CDATA[Background: Congenital hypothyroidism (CH) is a condition of thyroid hormone deficiency that occurs at birth. The TSH (thyroid-stimulating hormone) test is crucial for diagnosing hypothyroidism. CH is known to cause prolonged unconjugated hyperbilirubinemia. Therefore, this study aimed to analyze the relationship between TSH and indirect bilirubin levels in neonates suspected of having jaundice. Methods: This is a non-experimental, retrospective study conducted at Lombok Dua Dua Lontar Mother and Child Hospital in Surabaya. The study involved data collection on neonates aged 2–7 days suspected of jaundice, whose TSH and indirect bilirubin levels were measured between November 2022 to April 2024. Results: Among 100 neonates, 62% were aged 2-4 days, while 38% were aged 5-7 days. The majority were male (56%), with female comprising 44%. Of the 100 neonates, only 1 (1%) had borderline TSH levels, while 99% had normal TSH levels. Hyperbilirubinemia was observed in 94% of the neonates, while 6% had normal indirect bilirubin levels. Statistical analysis using the Spearman correlation showed no significant link between TSH and indirect bilirubin levels (p = 0.802). Conclusions: While this study did not find a clear connection between TSH and indirect bilirubin levels in neonates suspected of having jaundice, one case of borderline TSH was identified. This neonate required referral to pediatric endocrinology, as untreated congenital hypothyroidism can lead to mental retardation. Despite limited research linking TSH and bilirubin levels in jaundiced neonates, routine screening for congenital hypothyroidism using TSH testing should be reconsidered. Future studies could benefit from focusing on specific causes of neonatal jaundice to help narrow down research questions in this area.]]>
<![CDATA[Gambaran Pemeriksaan Hepatitis B Surface Antigen (HBsAg) pada Ibu Hamil di Puskesmas Wringinanom, Gresik ]]>
<![CDATA[Niswata, Navila Arfianti]]>;
<![CDATA[Suhariyadi, Suhariyadi]]>;
<![CDATA[Anggraini, Anita Dwi]]>;
<![CDATA[Woelansari, Evy Diah]]>
<![CDATA[ Medica Arteriana (Med-Art) Vol 6, No 2 (2024): Desember 2024 ]]>
Publisher : <![CDATA[University of Muhammadiyah Semarang ]]>
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DOI: 10.26714/medart.6.2.2024.50-55
<![CDATA[Latar Belakang: Penularan virus hepatitis B (VHB) secara vertikal masih menjadi faktor utama dari penularan penyakit hepatitis B di negara dengan tingkat endemisitas infeksi tinggi termasuk Indonesia. Berdasarkan laporan Deteksi Dini Hepatitis B (DDHB) ibu hamil tahun 2022 di Provinsi Jawa Timur, prevalensi dari hepatitis B pada ibu hamil di Kabupaten Gresik adalah 3,24% yang termasuk dalam prevalensi HBsAg 5 tertinggi di Provinsi Jawa Timur. Pada tahun 2023, diketahui 22 ibu hamil dengan hasil pemeriksaan positif HBsAg di Puskesmas Wringinanom, Gresik. Skrining pada ibu hamil adalah cara terbaik untuk mencegah virus hepatitis B menyebar dari ibu ke bayi. Sehingga tujuan penelitian ini untuk mengetahui gambaran pemeriksaan HBsAg pada ibu hamil di Puskesmas Wringinanom Gresik.Metode: Penelitian ini bersifat deskriptif dengan sampel penelitian adalah data primer dari pemeriksaan sampel serum darah pasien ibu hamil dari trimester pertama, kedua, dan ketiga yang berkunjung ke Puskesmas Wringin-anom Gresik untuk melakukan pemeriksaan ANC terpadu (Antenatal Care) dan melakukan pemeriksaan HBsAg. Teknik pengambilan sampel menggunakan purposive sampling sebanyak 30 orang ibu hamil. Metode yang digunakan adalah imunokromatografi dengan strip HBsAg rapid test dengan analisa data menggunakan uji distribusi frekuensi.Hasil: Ibu hamil dengan hasil pemeriksaan positif HBsAg adalah sebanyak 3 orang (10%) dan hasil pemeriksaan negatif HBsAg adalah 27 orang (90%). Ibu hamil dengan positif HBsAg berdasarkan karakteristik ditemukan 67% pada ibu hamil dengan interval umur 26-35 tahun dan pada trimester I. Ditemukan 33% pada interval umur 36-45 tahun dan pada trimester III. Serta ditemukan 100% pada ibu multigravida.Kesimpulan:Terdapat 10% pemeriksaan HBsAg dinyatakan positif pada Ibu hamil di Wilayah Puskesmas Wringinanom Gresik.]]>
