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Suwitra, I Nyoman

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Author-ID : 8829442

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering

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The use of technical chlorine for seawater sterilization in the rearing of barramundi larvae Lates calcarifer Astuti, Ni Wayan Widya; Mastuti, Indah; Sedana, I Made; Sunarto, Sunarto; Mustakim, Mustakim; Suwitra, I Nyoman; Mujiono, Mujiono; Kurdi, Kurdi; Dewi, Luh Yuliani; Tantra, Kadek Mas; Katimin, Katimin; Shadiq, Ja'far; Muzaki, Ahmad
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan 2025: Special Issue ICFM
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.0.0.46920

Disease prevention is very important in the rearing of barramundi larvae, one of which is by sterilizing the seawater using technical chlorine. This study aimed to determine the effect of seawater sterlization use technical chlorine for rearing barramundi larvae on growth, survival, and water quality. The treatment in this study was different seawater used for water exchange during larval rearing. Seawater was sterilized with 25 ppm technical chlorine for 24 hours. Before use, the water was neutralized using 12.5 ppm sodium thiosulfate. As a control in this study, direct seawater or untreated sea water was used for water exchange. The study was conducted with 3 replications. The parameters observed in this study were growth, survival, virus monitoring, growth variation, and water quality. Sterilization of seawater with 25 ppm technical chlorine used for rearing larvae of barramundi has no significant effect on growth, survival, growth variation and water quality of rearing larvae. The use of filtered seawater is recommended in the sterilization of seawater for rearing larvae of barramundi. Increasing the dose of chlorine or using chlorine which has a high active substance could increase effectiveness of water sterilization.Keywords:Technical chlorineSterilizationRearing larvaeBarramundiLates calcarifer

The use of technical chlorine for seawater sterilization in the rearing of barramundi larvae Lates calcarifer Astuti, Ni Wayan Widya; Mastuti, Indah; Sedana, I Made; Sunarto, Sunarto; Mustakim, Mustakim; Suwitra, I Nyoman; Mujiono, Mujiono; Kurdi, Kurdi; Dewi, Luh Yuliani; Tantra, Kadek Mas; Katimin, Katimin; Shadiq, Ja'far; Muzaki, Ahmad
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan 2025: Special Issue ICFM
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.0.0.46920

Disease prevention is very important in the rearing of barramundi larvae, one of which is by sterilizing the seawater using technical chlorine. This study aimed to determine the effect of seawater sterlization use technical chlorine for rearing barramundi larvae on growth, survival, and water quality. The treatment in this study was different seawater used for water exchange during larval rearing. Seawater was sterilized with 25 ppm technical chlorine for 24 hours. Before use, the water was neutralized using 12.5 ppm sodium thiosulfate. As a control in this study, direct seawater or untreated sea water was used for water exchange. The study was conducted with 3 replications. The parameters observed in this study were growth, survival, virus monitoring, growth variation, and water quality. Sterilization of seawater with 25 ppm technical chlorine used for rearing larvae of barramundi has no significant effect on growth, survival, growth variation and water quality of rearing larvae. The use of filtered seawater is recommended in the sterilization of seawater for rearing larvae of barramundi. Increasing the dose of chlorine or using chlorine which has a high active substance could increase effectiveness of water sterilization.Keywords:Technical chlorineSterilizationRearing larvaeBarramundiLates calcarifer

POTENSI ANTIPARASITIK HERBAL EKSTRAK HERBAL TERHADAP LINTAH LAUT (ZEYLANICOBDELLA ARUGAMENSIS) PADA JUVENIL IKAN KERAPU HIBRIDA CANTANG (Epinephelus fuscoguttatus X E. lanceolatus) Mastuti, Indah; Sadewa, Ketut M. Arya; Ansari, Mohamad; Haryanto, Slamet; Zailani, Ahmad; Suwitra, I Nyoman; Ariani, Ni Kadek; Kurdi, Kurdi; Miniartini, Made; Mustaqim, Mustaqim; Sedana, I Made; Sunarto, Mujiono; Astuti, Ni Wayan Widya; Mahardika, Ketut
Media Akuakultur Vol 20, No 1 (2025): Juni 2025
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.20.1.2025.1-13

Lintah laut adalahektoparasit yang sangat merugikan bagbudidaya ikan kerapu di Bali Utara. Penelitian ini bertujuan menggali potensi antiparasitik jenis 76 tanaman dalam mengendalikan infeksi lintah laut (Zeylanicobdella arugamensis) pada juvenil ikan kerapu hibrida cantang (Epinephelus fuscoguttatus x E. lanceolatus). Sebanyak 76 jenis tanaman diekstrak menggunakan pelarut air laut steril untuk uji in vitro tahap 1 sebagai skrining awal, dilanjutkan dengan uji in vitro tahap 2 dan uji in vivo tahap 1 dan tahap 2. Uji in vivo dilakukan dengan merendam lintah laut dalam ektrak herbal 1000 ppm selama dua jam. Hasil uji in vitro tahap 1 mendapakan 11 tanaman berpotensi antiparasitik, yaitu lada putih, lada hitam, bunga cengkeh, lengkuas, cabai Jawa, jahe hitam, kulit buah manggis, daun sawo Manila, daun delima, daun brotowali dan batang brotowali. Berdasarkan uji tersebut dilakukan uji in vitro tahap 2 untuk mengetahui konsentrasi minimal yang melemahkan lintah laut. Uji in vitro tahap 2 menunjukkan bahwa konsentrasi minimum (500 ppm) hanya diperoleh dari tanaman lada putih. Uji in vivo dilakukan dengan menggunakan ikan kerapu cantang yang yang terinfeksi lintah laut. Uji in vivo tahap 1 menunjukkan bahwa sembilan (lada putih, lada hitam, bunga cengkeh, lengkuas, cabai Jawa, kulit buah manggis, daun sawo Manila, daun delima, daun brotowali) dari sebelas herbal tersebut belum mampu melepaskan lintah laut namun mematikan bagi ikan. Sedangkan uji in vivo tahap 2 menunjukkan sekaligus menyimpulkan bahwa ekstrak herbal, yaitu jahe hitam (Curcuma aeruginosa Roxb.) dan daun brotowali efektif (Tinospora cordifolia) dalam air laut konsentrasi 1000 ppm.Sea leeches are the major ectoparasites that infect grouper fish. This study explores the antiparasitic potential of 76 plants in controlling sea leech. The plants were extracted using sterile seawater and used in the in vitro test 1 (initial screening), followed by in vitro test 2 and in vivo test 1 and 2. The in vivo test was carried out by soaking sea leeches in 1000 ppm herbal extractsfor two hours. The results of the in vitro test 1 found that 11 plants had antiparasitic potential (white pepper, black pepper, clove flowers, galangal, Javanese chili, black ginger, mangosteen peel, Manila sapodilla leaves, pomegranate leaves, brotowali leaves and brotowali stems). Based on initial test, the in vitro test 2 was conducted to determine the minimum concentration. The in vitro test 2 showed that the minimum concentration (500 ppm) was obtained from white pepper . The in vivo test was conducted using infected fish. The in vivo test 2 showed that nine (white pepper, black pepper, clove flowers, galangal, Javanese chili, mangosteen peel, Manila sapodilla leaves, pomegranate leaves, brotowali leaves) herbals were not able to release leeches but deadly to the fish. Meanwhile, the in vivo test 2 concluded that black ginger (Curcuma aeruginosa Roxb.) and brotowali leaves (Tinospora cordifolia) in sea water at a concentration of 1000 ppm were effective in releasing leeches within 10 minutes and non toxic to fish.

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