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All Journal Bioscience BERKALA SAINSTEK Biosfer: Jurnal Tadris Biologi Jurnal Biologi Universitas Andalas BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Pharmaceutical And Biomedical Sciences Journal (PBSJ) JOMPA ABDI: Jurnal Pengabdian Masyarakat BIO PALEMBANICA Berita Biologi
Swandi, Monica Kharisma
Universitas Bangka Belitung
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Isolation, Characterization and Activity Test of Soil Origin Bacteria Amilage Swandi, Monica Kharisma
Biosfer: Jurnal Tadris Biologi Vol 11 No 2 (2020): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v11i2.6015

Abstract

This study aimed to obtain amylase-producing potent bacteria from soil and test the amylase activity produced. Soil samples were taken from the Biological Education and Research Forest, Andalas University. The isolation was done by using the stratified dilution technique on agar media. The screening of amylase activity employed the qualitative and quantitative tests on agar starch. From 8 isolated amylolytic bacteria, there were three isolates with amylolytic potential. The results of characterization and identification based on Bergey's Manual of Determinative Bacteriology show that isolates A4, A1, and A6 belonged to the genus Bacillus, Corynebacterium, and Klebsiella. The bacteria obtained can then be produced and optimized for the needs of industrial enzymes.
CHARACTERISTICS AND POTENTIAL OF MICROPLASTIC DEGRADING BACTERIA FROM LOCAL MICROORGANISMS OF LERI ARUK FROM BANGKA BELITUNG Desi, Desi; Syafiqo, Syafira Auliani; Olieviani, Marisa; Sari, Maya; Swandi, Monica Kharisma
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14520

Abstract

One of the efforts to reduce the impact of microplastic pollution is through local microorganisms (MOL). Practical and applicable MOL can be obtained from local materials such as leri aruk Bangka Belitung. This study aims to test the characteristics of various MOL formulations from leri aruk typical of Bangka Belitung as candidates for microplastic degradation agents and to characterize the bacteria. This study was conducted in several stages, namely sampling, making various MOL formulations, testing MOL based on physical, chemical and biological properties, isolation and characterization of microplastic degrading bacteria and testing the ability of bacteria to degrade microplastics. The results of the study obtained the best formulation results based on physical, chemical and biological properties, namely in formulation 2. The isolation results obtained 4 isolates that have the potential to degrade microplastics, namely isolates B13, B18, B19 and B20.Morphological and biochemical characterization of bacteria showed that isolates B13 and B19 were LactoBacillus, isolate B18 was Bacillus and isolate B20 was Escherichia. The consortium of 4 isolates can degrade microplastics by 7.14% for 1 week on LDPE plastic.
Karakteristik Berbagai Formulasi Mikroorganisme Lokal (MOL) dan Pengaruhnya terhadap Pertumbuhan Tanaman Kangkung Darat (Ipomoea reptans (L.) Poir) Swandi, Monica Kharisma; Jeniver, Jely; Nur Milah, Siti Ade; Safitri, Mutiara; Asyyifa, Inayah; Irawati, Irawati; Aliya, Putri; Khotimah, Khusnul; Sari, Astri Dian; Putri, Julian Eka; Sari, Nadila Puspita; Fatansyah, Firzan; Harita, Efita Karunia; Wiriyanti, Lesta Ayu; Indah Suryani, Putri Ayu
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 8 No 1 (2023): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekotonia.v8i1.4161

Abstract

Local microorganisms (MOL) contain fermented solutions made from various local resources. MOL solution has the potential to be used as liquid organic fertilizer because it contains microorganisms that can increase plant growth. This study aims to determine the characteristics of MOL based on different sources of microorganisms obtained from food ingredients typical of Bangka Belitung, as well as to determine their effectiveness on the growth of Ipomoea reptans (L.) Poir. This experimental study used a two-factorial completely randomized design (CRD), namely the type of MOL (without giving MOL (M0), MOL made from Bangka shrimp paste (M1), MOL made from rusip (M2), and MOL made from kitchen waste (M3)) and MOL dose (0% (D0), 15% (D1), 25% (D2), and 50% (D3)). Each treatment was repeated 2 times with a total of 32 experimental units. The variables observed were the characteristics of MOL after 1 week of incubation and the effect of giving MOL on the growth of Ipomoea reptans (L.) Poir 14 days after planting (HST). Data were statistically analyzed using SPSS software version 25 using the analysis of variance (ANOVA) method and Duncan's multiple range test (DMRT) with a 95% confidence level. The results showed that all MOL treatments were successfully fermented with characteristics of sour aroma, liquid texture, produce CO2, acidic pH, and presence of bacteria and mold. The results of ANOVA showed that the factor of giving the type of MOL and the interaction both factor had a significant effect on all observation parameters. However, the MOL dose factor did not significantly affect all observation parameters. Treatment of interaction the type and dose of MOL M2D1 can increase the response of the number of leaves, plant wet weight, plant dry weight, shoot dry weight, and root dry weight of Ipomoea reptans (L.) Poirs. The teratment of M1D3 increased the height response of Ipomoea reptans (L.) Poirs.
ISOLASI, KARAKTERISASI, DAN POTENSI ENZIMATIS BAKTERI ASAL TANAH SAMPAH DI TEMPAT PEMBUANGAN AKHIR SEBAGAI KANDIDAT AGEN BIODEKOMPOSER Swandi, Monica Kharisma; Ropalia; Anggraeni
Berita Biologi Vol 24 No 1 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.2285

Abstract

Bakteri sebagai organisme dekomposer memiliki kemampuan mendegradasi bahan-bahan organik untuk kerberlangsungan siklus biogeokimia di alam. Berbagai bakteri pendegradasi telah dilaporkan berhasil diisolasi dari berbagai sampel, namun masih terbatas pada potensi enzimatis tertentu sehingga proses dekomposisi tidak optimal. Oleh karena itu, pentingnya penelitian ini dilakukan dengan tujuan mengisolasi, mengkarakterisasi dan mengidentifikasi isolat bakteri penghasil enzim ekstraseluler (selulase, amilase, protease, lipase), dan menganalisis potensi isolat bakteri enzimatis sebagai agen biodekomposer. Tahapan penelitian dimulai dari pengambilan sampel dan isolasi bakteri. Isolat yang diperoleh diuji potensinya sebagai biodekomposer melalui uji aktivitas enzim secara kualitatif, meliputi selulase, amilase, protease, dan lipase. Selanjutnya dilakukan penapisan isolat berdasarkan uji hemolisis. Isolat terpilih kemudian dilakukan penentuan kurva tumbuh, dikarakterisasi dan diidentifikasi. Sebanyak 22 isolat bakteri berhasil diisolasi dari TPA Parit Enam Kota Pangkalpinang. Hasil penapisan menunjukkan sebanyak 8 isolat memiliki kemampuan keempat aktivitas enzim sekaligus, dan dari 8 isolat hanya 4 isolat yang tergolong tidak patogen yaitu isolat BS 2.1, BS 2.2, BS 3.3, dan BS 3.7. Keempat isolat tersebut dapat diproduksi pada fase perbanyakan dalam rentang jam ke-4 hingga ke-18. Berdasarkan karakterisasi morfologi koloni, morfologi sel dan biokimia, isolat BS 2.1, BS 2.2, BS 3.3, dan BS 3.7 dapat diduga sebagai Pseudomonas, Streptococcus, Lactobacillus, dan Bacillus.
Co-Authors Aliya, Putri Anggraeni Asyavira, Gita Cindy Asyyifa, Inayah Desi Desi, Desi Effendi, Zahrial Fatansyah, Firzan Firsty Vanezza Gabriela Fitri Ulandari Frista Chairunnisa Harita, Efita Karunia Indah Suryani, Putri Ayu Irawati Irawati Jeniver, Jely khusnul khotimah Maya Sari Mega Febriyanti Muhammad Iqbal Nadhifah Raniah Neli Sulastri Nur Arifah Nur Azizah Nur Milah, Siti Ade Nurmiati Nurmiati Olieviani, Marisa Periadnadi Periadnadi Putri, Julian Eka Rama Pratama Ropalia, Ropalia Safitri, Mutiara Salmi Salmi Salmi, Salmi Sari, Astri Dian Sari, Diah Komala Sari, Nadila Puspita Syafiqo, Syafira Auliani Widayanti, Gusti Ayu Wiriyanti, Lesta Ayu